Labshake search
Citations for Millipore Sigma :
8651 - 8700 of 10000+ citations for Recombinant Human Pentraxin 3 Long His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141) ...
-
bioRxiv - Cell Biology 2024Quote: ... the chips were incubated with 0.1% (m/v) gelatin combined with 20 µg/ml human plasma fibronectin (Sigma-Aldrich, 341635) in PBS at 37°C for 1 hour ...
-
bioRxiv - Immunology 2024Quote: Single cell solution was obtained from human normal donor and IPF lungs after enzymatic digestion with 10X Liberase (Sigma, 5401127001) and DNaseI (9003-98-9 ...
-
bioRxiv - Neuroscience 2024Quote: ... Levels of individual cytokines were determined using a Milliplex bead assay kit (Milliplex MAP human cytokine/chemokine magnetic bead panel, #MCYTOMAG-70K; Millipore) and involving the Luminex MAPIX system with data analysis using xPONENT 4.2 software (Luminex) ...
-
bioRxiv - Cell Biology 2024Quote: ... Terminal deoxy-nucleotidyl transferase dUTP nick end labeling (TUNEL) was performed on transduced human islets (Apoptag In situ Apoptosis Detection Kit; Millipore) as per the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2024Quote: ... Aβ and pTau levels in the organoids were quantified with Milliplex human amyloid beta and tau kit (Millipore HNABTMAG-68K) on the Luminex ...
-
bioRxiv - Cancer Biology 2024Quote: ... CWR22 (primary prostate tumour) human prostate cancer cell lines were obtained from ATCC and grown in RPMI-1640 (Sigma Aldrich) and supplemented with 1% L-Glutamine (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: Binding of cGAS and cytoplasmic mtDNA in human and mouse brain sections was determined by a PLA kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: Total protein was extracted from 20-30 mg human frontal cortex using RIPA buffer (PierceTM RIPA Buffer, ThermoScientific, 89901) supplemented with 0.05% CHAPS (Sigma, C3023), 1X complete protease inhibitor (Sigma-Aldrich ...
-
bioRxiv - Immunology 2024Quote: ... and perfusate levels of 71 analytes using two separate panels as per the manufacturer’s instructions for use: (1) the Human Cytokine 48-Plex Discovery Assay® (HD48; Millipore MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel Immunology Multiplex Assay ...
-
bioRxiv - Neuroscience 2024Quote: The cortex tissue samples from mice or human were lysed in PBS along with PDE inhibitor (IBMX) and PIC (SIGMA) with homogenizer followed by centrifugation at 12500 rpm for 20min at 4°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... under air-liquid interface conditions in Greens+ keratinocyte proliferation media (Greens media supplemented with 10 ng/mL human EGF (Sigma)) ...
-
bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
-
bioRxiv - Cell Biology 2024Quote: Cells were grown to 70-80% confluency on surfaces were coated with 10μg/ml of purified human fibronectin (SIGMA-Aldrich). Orbital shear stress was applied to the cells for 18-24 h at 37°C and 5% CO2 ...
-
bioRxiv - Microbiology 2024Quote: Human fecal samples (10-20 mg) were resuspended in supplemented Brain Heart Infusion (BHI, Sigma-Aldrich, St. Quentin Fallavier, France) as described in(Motta et al. ...
-
bioRxiv - Microbiology 2024Quote: ... albumin from chicken egg white (44 kDa) and γ-globulins from human blood (160 kDa) were purchased from Sigma-Aldrich.
-
bioRxiv - Immunology 2024Quote: BMMCs were sensitized for Ag stimulation with dinitrophenol (DNP) coupled to human serum albumin (HSA) (DNP-HSA, Sigma-Aldrich, #A6661) by pre-loading the FcεRI with 0.15 µg/ml IgE (clone SPE-7 ...
-
bioRxiv - Microbiology 2020Quote: ... The sample was diluted and purified with a 3 kDa Amicon Ultra centrifugal filter (Millipore, Temecula, CA, USA) to remove low molecular weight impurities ...
-
bioRxiv - Microbiology 2020Quote: ... plates supplemented with 3% sheep blood (iTek StPaul MN) and 20 μg/mL neomycin (Sigma St Louis MO) and then grown overnight at 37°C in a 5% CO2 humidified incubator ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were fixed in 3% formaldehyde solution (37 wt.% in H2O, 252549, Sigma-Aldrich, St. Louis, MO) with 0.1% glutaraldehyde solution (25% in H2O ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cell lines were regularly passaged every 3-4 days by dissociation with Trypsin-EDTA 0.25% (Sigma Aldrich). The cell lines were regularly subjected to monthly screening for mycoplasma infection ...
-
bioRxiv - Cancer Biology 2021Quote: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was purchased from Sigma-Aldrich (L’Isle-d’Abeau-Chesnes, France). Antibodies against phospho-IκBα Ser32 (#2859) ...
-
bioRxiv - Immunology 2021Quote: ... After 3 washes in TBST the blot was revealed using the EC™ Prime Western Blotting System (Sigma). The membrane was imaged using a Chemidoc MP imaging system from BioRad.
-
bioRxiv - Cell Biology 2019Quote: ... larvae were washed 3 times with 0.1% PBS-Tween20 and incubated in 0.1% w/v collagenase type 1A (Sigma) in PBS at 37°C for 1.5 hours ...
-
bioRxiv - Developmental Biology 2021Quote: ... Alive embryos older than 16 ss were additionally mounted with 0.016% ethyl 3-aminobenzoate methanesulfonate salt (Tricaine, Sigma) in the LMA and added to the E3 medium to prevent movement during imaging ...
-
bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
-
bioRxiv - Molecular Biology 2020Quote: ... cells were washed twice with 3% BSA in PBS 1X and stained with 5μg/mL Hoechst (Sigma Aldrich). Samples’ images were acquired and analysed by high content fluorescence microscope Operetta® (PerkinElmer ...
-
bioRxiv - Microbiology 2019Quote: Non- and PUUV-Suo infected Mygla.REC.B cells were exogenously stimulated with TLR-3 ligand polyI:C (10 µg/ml, Sigma Aldrich) or Sendai virus (multiplicity of infection 1 ...
-
bioRxiv - Molecular Biology 2021Quote: A2780-DNA329 and OVCAR-3 (ATCC) ovarian cancer cell lines were cultured in RPMI-1640 medium (R8758, Sigma). Ovarian cancer COV362 (ECACC) ...
-
bioRxiv - Cell Biology 2021Quote: ... washed 3 times in 1X PBS and incubated for 5 min in 50mM ammonium chloride (Sigma-Aldrich, 254134) before mounting on cavity blades (Marienfeld ...
-
bioRxiv - Neuroscience 2021Quote: ... 3 ml of an enzyme cocktail [1 mg/ml collagenase A (#11088793001, Sigma-Aldrich, St. Louis, MO, USA) and 0.1 mg/ml elastase (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: Prior to training rats were randomly assigned to receive either sham surgery or intracranial bilateral lesions to the region of interest under 2-3% isoflurane anaesthesia with local application of bupivacaine (2mg/kg s.c. at 0.8ml/kg; Sigma) at the incision site ...
-
bioRxiv - Neuroscience 2021Quote: ... blocking and permeabilization was performed with DPBS + 3% BSA + 0.1% Triton X-100 (Millipore Sigma cat. no. X100) for 30 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... shShank3 injected mice were randomly infused with 2µL (500nL m-1) of vehicle (∼3 % dimethyl sulfoxide (DMSO, Sigma) diluted in aCSF ...
-
bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %). The treatment was counterbalanced between trials ...
-
bioRxiv - Neuroscience 2021Quote: ... diluted in aCSF) or with 2µL Trpv4 antagonist (HC-067047, Sigma 2 µg diluted in aCSF-DMSO ∼3 %).
-
bioRxiv - Molecular Biology 2021Quote: ... Denmark) as well as the BKCa blockers paxilline (3 μmol/L with 0.03% DMSO, Sigma-Aldrich, Darmstadt, Germany) and iberiotoxin (100 nmol/L ...
-
bioRxiv - Immunology 2021Quote: ... The detached peptides were separated from HLA molecules using 3 kDa cut-off centrifugal ultrafilters (Millipore, MO, USA), and then concentrated using vacuum centrifugation.
-
bioRxiv - Molecular Biology 2019Quote: ... Expression of constructs was suppressed by addition of 0.5 µM and indole-3-acetic acid (IAA; Sigma, I5148). 2i F1 hybrid 129/Sv:Cast/Eij mESCs cells were cultured for 2 weeks on primary mEFs in 2i ES cell culture media ...
-
bioRxiv - Biochemistry 2019Quote: ... the probes were transferred into a 0.1% (v/v) solution of 3-aminopropyltrimethoxysilane (APTES) (Sigma, St. Louis, MO) in toluene for 2 h ...
-
bioRxiv - Immunology 2019Quote: ... 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Sigma-Aldrich (Saint Louis, MO, USA) and GeneJuice® Transfection Reagent (Novagen ...
-
bioRxiv - Molecular Biology 2019Quote: ... To create the Tmem98A193P allele Edit-R crRNA (sequence 5’-CCAAUCACUGUCUGCCGCUG-3’) (Dharmacon) was annealed to tracrRNA (Sigma) in IDT Duplex buffer (IDT) ...
-
bioRxiv - Bioengineering 2019Quote: ... Chips fabricated with polyester terephthalate (PET) track-etched membranes were assembled using (3-Glycidyloxypropyl) trimethoxysilane (GPTMS; Sigma, 440167) and (3-Aminopropyl ...
-
bioRxiv - Microbiology 2019Quote: ... cells were washed 3 x in ice-cold choline-buffer and lysed in 0.5% Triton X-100 (Sigma) in double-distilled water for 20 min at room temperature with gentle agitation ...
-
bioRxiv - Molecular Biology 2020Quote: ... The eluted nsp7 sample was concentrated with a 3 kDa molecular weight cut-off Centrifuge Filter (Millipore Corporation) and then size-separated by a Superdex S75 10/300 GL column in 25 mM HEPES pH 7.4 ...
-
bioRxiv - Cancer Biology 2020Quote: ... cells were split into 3 replicates containing 6.6 million cells each and treated with either DMSO (Sigma-Aldrich) or 40nM erlotinib (Selleckchem) ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sections were then stained at room temperature in 0.05 M Tris-HCl pH 7.6 buffer containing 0.5 mg/ml 3-39 diaminobenzidine (DAB, Sigma Aldrich) and 0.01% hydrogen peroxide.
-
bioRxiv - Neuroscience 2019Quote: ... Sections were washed with 1X TBS 3 × 5 minutes followed by treatment with 0.05% 3,3’-Diaminobenzidine (Sigma Aldrich) and 0.01% H2O2/1X TBS for 20 minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... The plates were washed 3 times with pre-cooled PBS prior to the addition of L-DOPA (Sigma). After incubation in L-DOPA for 1 day ...
-
bioRxiv - Biochemistry 2019Quote: ... Isolated protein was then buffer exchanged with 0.5 M Triethyl ammonium bicarbonate (pH 8.5) using a 3 kDa cutoff centrifugal filter (Amicon, Millipore). Protein was then estimated using Bradford’s reagent (Sigma ...