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Citations for Millipore Sigma :
8251 - 8300 of 10000+ citations for Rat Epithelial membrane protein 1 EPN3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... using a Milliplex Rat Gut Hormone Panel 96-well plate assay as described by the manufacturer (#RMHMAG-84K-06; EMD Millipore Corporation, Billerica, MA). Data was collected with MAGPIX with xPONENT software (Luminex ...
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bioRxiv - Neuroscience 2021Quote: ... corresponding to the external Ig-like domain) diluted in sterile PBS and emulsified in an equal volume of incomplete Freund’s adjuvant (IFA, Sigma; total volume 100µl per rat). IFA control rats were immunised with PBS emulsified in an equal volume of IFA ...
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bioRxiv - Neuroscience 2020Quote: ... Cells (primary culture of rat ENS) and tissues from mice (hippocampus, ileum, proximal and distal colon) were lysed in RIPA lysis buffer (Merck Millipore, Fontenay sous Bois, France) containing phosphatase inhibitor cocktail II (Roche ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST before incubating overnight at 4°C in primary antibody solution (4% NGS in TBST) with rat anti-GFAP (Millipore Cat# 345860-100UG, RRID:AB_211868), rabbit anti-GFAP (1:2000 ...
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bioRxiv - Physiology 2020Quote: ... The hydrolyzed TMOS (100 μl) was added to 900 μl of 10 μM of pooled siRNA against rat FL2 (siRNA from Sigma-Aldrich (St Louis, MO): SASI_Rn02 00314854 ...
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bioRxiv - Physiology 2021Quote: ... the experimental rats (body mass 171 to 203 g) were injected subcutaneously with 60 mg monocrotaline in saline/kg body mass (Sigma-Aldrich, St Louis, MO) to induce pulmonary hypertension ...
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bioRxiv - Bioengineering 2022Quote: ... As previously described,29 male Sprague Dawley (SD) rats (200-250g) were injected with monocrotaline (MCT) (60 mg/kg, Sigma Aldrich, Oakville, ON, Canada) by intra peritoneal (i.p. ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were loaded on an 8% Tris/glycine gel and subjected to Western blot analysis using different primary antibodies (anti-c-myc (A-14, sc-769, Santa Cruz, Nunningen, Switzerland and HA antibody, rat, 3F10, Sigma-Aldrich, Munich, Germany).
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bioRxiv - Neuroscience 2022Quote: Primary rat glial cells (astrocytes and microglia) and neuroblastoma cells were fixed for 15 min in 4% paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and washed with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Four to five-week-old donor rats were superovulated by intraperitoneal injection of pregnant mare serum gonadotropin (30 units, G4877, Sigma-Aldrich, St. Louis, MO), followed by an intraperitoneal injection of human chorionic gonadotropin (30 units ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Plasma and dorsal hippocampal CA1 cytokines were assessed using a MILLIPLEX® Rat Cytokine/Chemokine Magnetic Bead Panel (Millipore Sigma Cat. No. RECYTMAG-65K) customized for detection of pro-inflammatory (TNF-α ...
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bioRxiv - Microbiology 2023Quote: ... All well plates and culture flasks were coated prior to seeding with 7 µg/mL rat tail tendon collagen I (Sigma-Aldrich, St. Louis, MO) and rinsed with PBS 3 times ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mL/well cell-virus suspension was seeded into 50 µg/mL rat tail collagen type I (Sigma Aldrich, St. Louise, MO USA) coated 6-well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... 167 mM NaCl) and precleared for 2 h with 250 μL of previously blocked 50% protein-A and protein-G beads (Sigma P-7786 and P-3296 respectively). Blocking was achieved by incubating the beads with 0.5 mg/ml of Ultrapure BSA and 0.2 mg/mL of salmon sperm DNA for 4 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... For p65 IP the lysates were then incubated with 2 μg of antibody CST ref #6956 or CST ref# 8242) at 4 °C for 2 hrs before adding 20 μL of slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose) for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1 x 106 stable cells using the GenElute mammalian genomic DNA kit (G1N350, Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... A tertiary streptavidin-HRP antibody (a part of the Mouse ExtrAvidin® Peroxidase Staining Kit from Sigma at 1:1000) was added for 2 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... the MILLIPLEX® MAP 6-plex Bcl-2 Family Apoptosis Panel 1 Magnetic Bead Kit (Millipore: Cat. No. 48-682MAG) was utilized ...
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bioRxiv - Pathology 2019Quote: ... treated with opsonized RBCs (1:10) and intracellular ROS was determined with a fluorometric intracellular ROS kit (Sigma-Aldrich, MAK142). Stained cells were visualized and captured using Leica DMI6000B with Cy5 filter set ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Plant Biology 2021Quote: The coding sequences of most of the 44 non-pseudogene AtUMAMIT were amplified by PCR (oligonucleotide sequence is provided in Supplemental Table 1) by using the KOD Hot Start DNA Polymerase kit (Novagen) from a mix of reverse-transcribed RNA extracted from seedlings and inflorescences ...
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bioRxiv - Genomics 2020Quote: CTCF Chromatin immunoprecipitation (ChIP) was performed on 1 × 107 Ter119+ erythroid cells using a ChIP Assay Kit (Millipore: 17-295) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1×105 cells were used to measure NADPH and NADP+ levels using a NADP+/NADPH colorimetric quantitation kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... or doxycycline-treated (Dox) (1 µg/ml for 6 days) AW13516-shNPM1-luc+ cells using the GenElute Mammalian Total RNA Miniprep Kit (Sigma) followed by On-Column DNase I Digestion as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... referred to here as hCMEC were obtained from Millipore and were maintained in an EndoGRO-MV complete medium kit supplemented with 1 ng/ml fibroblast growth factor-2 (FGF-2; Millipore) (81–84) ...
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Neuroscience 2019Quote: The anterior half of cortices from P0 mice were dissociated then cross-linked with 1% formaldehyde and processed using EZ-ChIP kit (Millipore). Chromatin fragments bound by endogenous mouse SOX5 were pulled down by anti-SOX5 (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined by LV Lentiviral Titer kit (MoBiTec) and viruses where used at a Multiplicity of infection of 1 – 5 using polybrene (H9268, Sigma) at a concentration of 2 μg/ml.
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bioRxiv - Bioengineering 2022Quote: ... These oligonucleotides were used to create a phage library representing all oligopeptides using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol (Harhala et al. ...
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bioRxiv - Pathology 2022Quote: ... The incubation was followed by three 5 min washes and incubation with PLA probes anti-goat PLUS (DUO92003) and anti-rabbit MINUS (DUO92005) for 1 h at 370C as recommended in the Duolink PLA kit (Sigma). After three washes and cells were incubated with the hybridization solution containing DNA ligase for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μM of carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone (FCCP) (Seahorse XF Cell Energy Phenotype Test Kit from Agilent or Sigma Aldrich), and 1 μM of antimycin A and rotenone (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... The abundance of cytokines was determined using a MILLIPLEX Mouse 32-Plex Cytokine Panel 1 kit (Millipore # MCYTMAG-70K-PX32) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2019Quote: ... Expressed proteins were detected by immunoblotting with anti-FLAG (Sigma, #F1804), anti-cMyc (Fujifilm Wako ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-protein complexes were eluted using Flag peptide (Millipore, Cat # F3290) at 150 µg/ml by incubating the beads at room temperature for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... FN3 proteins were expressed in Rosetta DE3 competent cells (EMD Millipore), lysed by sonication ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were resuspended in 100 mM TEAB buffer (triethylammonium bicarbonate, Sigma) and then digested by trypsin (Pierce MS grade ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were detected using either Luminata Crescendo Western HRP Substrate (Millipore) or ECL Western Blotting Analysis System (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... and included protein phosphatase inhibitor cocktails I and III (Sigma-Aldrich) in cell extracts ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration in the supernatant was measured using Bradford Reagent (Sigma), and equal amounts of protein were resolved by SDS-PAGE.
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bioRxiv - Genetics 2021Quote: ... The expressed proteins were partially purified on Q sepharose (Sigma q1126) and the fractions containing the protein of interest were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were reduced with 1.5 µmol of DTT (Sigma-Aldrich, Canada) for 20 minutes at 70 degrees Celsius ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells where then lysed with BugBuster protein extraction reagent (Novagen) for 30 min at room temperature ...
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bioRxiv - Immunology 2020Quote: A commercial protein-A affinity chromatography called PURE-1A (Sigma-Aldrich.) was used to purify anti-anti-SpA antibodies (Ab-3 ...
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bioRxiv - Developmental Biology 2020Quote: ... and protein concentration was determined using the Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× SDS Loading Dye (100 mM Tris pH 6.8 ...
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bioRxiv - Immunology 2022Quote: Protein lysate was extracted using CelLytic B Cell Lysis Reagent (Sigma) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... AID-tagged proteins were conditionally depleted using 250mM auxin (Sigma, I2886) stock dissolved in ethanol at a final concentration of 0.5mM ...
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bioRxiv - Molecular Biology 2021Quote: ... 40 µl of pre-washed protein A beads per sample (Sigma, equilibrated to lysis buffer as mentioned in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were transferred to Immobilon FL (EMD Millipore Corp IPFL00010) membrane in transfer buffer containing 10% methanol and 0.2% SDS ...