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Citations for Millipore Sigma :
601 - 650 of 7689 citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... except that each virus was added to SVOG3e/rtTA cells and selected with 0.5 μg/ml Puromycin (Sigma P8833) and 0.6 mg/ml Geneticin to create polyclonal stable cell lines SVOG3e/rtTA/V5 (V5 control cell line) ...
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bioRxiv - Cancer Biology 2020Quote: ... Viral mixes were created with an aliquot of virus into complete media and polybrene at 8μg/ml (Sigma-Aldrich) to have a target multiplicity of infection (MOI ...
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bioRxiv - Immunology 2019Quote: Media was recovered approximately 48-hr post-transfection and cell-free virus stocks were generated by filtering the media through 0.45 µM filters (Millipore). The TZM-bl indicator cell line was used to quantify the amount of infectious virus(Derdeyn et al. ...
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bioRxiv - Biochemistry 2019Quote: ... First-strand cDNA was synthesized from 2 µg of total RNA using Moloney Murine Leukemia Virus Reverse Transcriptase (Sigma) and oligo dT (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... The activated cells were added to the virus-bound plates after removing dead cells using Histopaque-1119 (Sigma-Aldrich), and centrifuged at 3200 × g for 90 min at 32°C ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lentivirally transduced by spinfection with centrifugation of cells with virus in the presence of 8μg/ml polybrene (Millipore) at 1,000xg for two hrs ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were seeded onto glass-bottom dishes and transduced with virus containing pEGFP_EB3 (gift from S. Godinho) Cells were treated with Eg5 (Kif11) inhibitor monastrol (67 μM, Sigma) for 2 hr ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RAMP1 expression achieved through transduction of virus containing RAMP1 MISSION TRC3 Open Reading Frame (ORF) plasmid (pLX_304) (Sigma, US) into RAMP2 KO-HUVECs ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... virus was mixed with 0.36 million cells in growth media supplemented with 8 μg/mL Polybrene (Sigma-Aldrich, H9268) and incubated overnight ...
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bioRxiv - Immunology 2021Quote: ... The virus particles in the supernatant were concentrated to 100μl using Amicon® Ultra-15 Centrifugal Filters (Sigma-Aldrich Chemicals Private Limited ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Microbiology 2021Quote: ... cell monolayers were washed twice with PBS before adding virus inoculum in DMEM supplemented with 1μg/ml of trypsin TPCK (Sigma) and no FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were transduced with optimal volume of lentiviral virus at 50% confluence in MV2 medium and 8μg/ml Polybrene (Sigma). After 24 h ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
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bioRxiv - Immunology 2023Quote: ... Next the pseudotyped virus-containing culture medium was diluted 50% in culture medium supplemented with 8μgml−1 polybrene (Sigma) and immediately applied onto MutuDC1 cells ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transduced with the respective virus particles in an equal ratio using 5 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552, Sigma-Aldrich, Darmstadt, Germany) based on the p24 antigen concentration in the supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Virus-containing supernatants were harvested after 48 h transfection and filtered by syringe through a 0.45 μM filter (Millipore) to eliminate cell contaminates ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transduced with the virus for 48 h in the presence of 4 µg/ml polybrene (Sigma H9268).
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bioRxiv - Microbiology 2023Quote: ... neutralized whole-virus samples were incubated in D2O buffer (99% deuterium oxide, Sigma 151882, supplemented with 120 mM NaCl). 40 μL D2O buffer was added to every 10 μL of neutralized virus (final D2O concentration 78% ...
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bioRxiv - Cancer Biology 2023Quote: ... subconfluent cultures were incubated with virus-containing supernatants in the presence of 4µg/ml polybrene (Sigma-Aldrich , TR-1003) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Microbiology 2023Quote: ... The medium for the culture of influenza A virus in MDCK cells (virus medium) was prepared by supplementing Minimal Essential Medium with 4.3% bovine serum albumin (BSA; Sigma) and Normocin.
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bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
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bioRxiv - Physiology 2024Quote: ... C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore) as shown previously40 ...
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bioRxiv - Genetics 2023Quote: ... RPE-1 cells were transduced with lentivirus at an MOI of 0.3-0.5 (usually between 50-100 µL of virus/well) and 10 µg/mL Polybrene (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Genetics 2021Quote: ... cells tested for several strains of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Molecular Biology 2021Quote: Promastigotes of Leishmania braziliensis strain M2903 (MHOM/BR/75/M2903) were cultured in M199 medium (Sigma–Aldrich) supplemented with 10% heat-inactivated fetal bovine serum as previously described (33) ...
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bioRxiv - Physiology 2020Quote: ... coli strain and RNA expression was induced by 1 mM isopropyl-β-d-thiogalactopyranoside (IPTG, Sigma-Aldrich). We used a slightly modified version of the established RNAi-by-feeding protocol [81] ...
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bioRxiv - Biophysics 2019Quote: ... or with 75 μg/mL lipopolysaccharide (LPS) from Pseudomonas aeruginosa (serotype 10.22, strain: ATCC 27316, Sigma-Aldrich) and incubated for 3 hours (37 °C ...
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bioRxiv - Microbiology 2019Quote: ... For each strain 60 µl lysate were incubated with 6 µl amyloglucosidase (200 U/ml, Sigma-Aldrich) (quantification of glucose stored as glycogen and free glucose ...
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bioRxiv - Neuroscience 2022Quote: CMTI-2 mouse embryonic stem cell line (derived from murine strain C57BL/6J, normal male genotype, Millipore) and their derivatives were used for all experiments ...
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bioRxiv - Immunology 2022Quote: Pa (M57-15 strain) was cultured on plates with LB broth with agar (#L7025-500TAB, Sigma-Aldrich) overnight at 37 °C from frozen stock ...
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bioRxiv - Plant Biology 2022Quote: ... Botrytis strain Bo5.10 was cultivated on commercial medium of potato dextrose agar (PDA; P2182, Sigma-Aldrich, USA). Botrytis plates were kept in dark at room temperature and transferred into 4°C when conidia were produced.
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bioRxiv - Microbiology 2022Quote: ... All bacterial strains were stored at - 80°C for long term storage in 25% sterile glycerol (Sigma). All cloning and strains were performed in dh10b genotype cells (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Procyclic forms (PCF; strain 427) were cultured in modified SDM-79 with 10 % heat-inactivated FCS (Sigma) at 27°C ...
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bioRxiv - Microbiology 2022Quote: ... the evolved strains were stored at -80°C in 1 ml of Phosphate Buffered Saline (PBS, Sigma) by adding 200 μL of 80% glycerol.
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bioRxiv - Cell Biology 2019Quote: ... yeast strains were grown in 80 μL of SD complete media supplemented with myo-inositol (Sigma-Aldrich) at 100 μg/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... Auxotrophic strains were supplemented with the required molecules at the following concentrations: 0.02 g.L−1 histidine (Sigma), 0.05 g.L−1 lysine (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... deletant strains were supplemented with geneticin (G-418 disulfate salt solution, Sigma-Aldrich, St. Louis, MO, USA) at a final concentration of 200 μg/mL.
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bioRxiv - Bioengineering 2020Quote: ... we cultured Escherichia coli strain JM109 in 250-mL 2× Yeast extract Tryptone growth medium (Sigma Aldrich) with 5 mM MgCl2 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Each replicate of the SSD1-HTP strain was filtered rapidly through 0.45µM nitrocellulose membrane filters (Millipore, HAWP09000) to collect the cells ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains expressing either RFP or GFP were separately cultured in Luria-Bertani (LB) medium (Sigma Aldrich) containing 1 µL/mL of chloramphenicol to retain the recombinant plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... baltica strain #1432 was grown at room temperature (RT) on agar plates (12 g sea salt (Sigma), 1 g yeast extract (Helicon) ...
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bioRxiv - Microbiology 2020Quote: ... All bacterial strains were stored at −80°C for long-term storage in 15% sterile glycerol (Sigma). The genotypes of E ...