Labshake search
Citations for Millipore Sigma :
8051 - 8100 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... we used the protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich, Deisenhofen, Germany). CHX inhibits eEF2-mediated translocation in eukaryotic ribosomes (Schneider-Poetsch et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... and fusion proteins purified according to manufacturer’s instructions (GE Healthcare or Novagen). Protein-protein binding buffer contained ...
-
bioRxiv - Neuroscience 2022Quote: ... RRID:AB_839504) and myelin basic protein (MBP; rat anti-MBP; #MAB386, Millipore; RRID:AB_94975).
-
bioRxiv - Neuroscience 2022Quote: Total protein concentrations were measured using the Pierce BCA method (Sigma, USA). Equivalent amounts of protein (40 mg per lane ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated using SDS-PAGE and transferred to PVDF membrane (Millipore). Immunoblotting was achieved using the indicated antibodies listed in the supplemental table 3 ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein bands were revealed with enhanced chemiluminescence reagent (211595, Millipore, Immobilon Crescendo) and images captured with a KwikQuant Imager (Kindle Biosciences) ...
-
bioRxiv - Neuroscience 2023Quote: ... Anti-Myelin Basic Protein antibody [12] (MBP) (Millipore; rat monoclonal, 1:500), Mouse anti-A2B5 (Invitrogen ...
-
bioRxiv - Physiology 2023Quote: ... Oxygen consumption rates were calculated and normalized for protein content by Novagen BSA assay (71285-3 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Eluted proteins were desalted using either Amicon Ultra centrifugation filters (Merc Millipore) or Zeba Spin desalting columns (Thermo Fisher scientific ...
-
bioRxiv - Immunology 2022Quote: ... samples were incubated in the presence of Protein A Sepharose beads (Millipore) for 1-2h ...
-
bioRxiv - Cell Biology 2024Quote: ... Protein bands were detected by Luminol-based enhanced chemiluminescence (EMD Millipore WBKLS0500). Immunoblots were captured on film and quantified using Image J.
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were visualized using chemiluminescence (Immobilon Western Chemiluminescent HRP Substrate, Millipore, #WBKLS0500) on Bio-Rad ChemiDoc (XRS+) ...
-
bioRxiv - Neuroscience 2022Quote: ... and the protein concentration determined with a BCA assay (Sigma, Deisenhofen, Germany). Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare ...
-
bioRxiv - Neuroscience 2022Quote: ... Transferring the protein from the gel to the PVDF membrane (Millipore #ISEQ00010). Membranes were blocked with 5% milk for one hour at room temperature and incubated with appropriate dilutions of primary antibody overnight at 4 °C ...
-
bioRxiv - Immunology 2022Quote: ... in the presence or absence of 0.5 mg/mL OVA protein (Sigma) in cRPMI at 37℃ in 5% CO2 incubator for 72 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein complex was concentrated using centrifuge filters (Amicon Ultracel-30K, Millipore) and subjected to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
-
bioRxiv - Physiology 2022Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
-
bioRxiv - Bioengineering 2022Quote: ... and then filtered through a 0.45-μm low protein binding membrane (Millipore). HEK293T cells were seeded into 12-well plates at approximately 40-50% confluency and 50 μl filtered virus-containing supernatant was added to the 12-well plates ...
-
bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
-
bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
-
bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
-
bioRxiv - Microbiology 2024Quote: ... Optimal protein expression and solubility was achieved in RosettaTM(DE3) pLysS (Novagen) for wild-type His::PfPKG ...
-
bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
-
bioRxiv - Biochemistry 2024Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
-
bioRxiv - Bioengineering 2024Quote: ... One hundred µL of protein samples or lysostaphin standards (SIGMA-L9043-5MG) diluted in 0.01% Tween 80 (SIGMA-P5188-100ML ...
-
bioRxiv - Cancer Biology 2024Quote: ... equal amounts of protein were run and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 5 % milk and incubated with the following antibodies ...
-
bioRxiv - Molecular Biology 2024Quote: ... Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
-
bioRxiv - Microbiology 2024Quote: ... RNA and proteins were extracted from the fractions using Trizol reagent (Sigma) as per manufacturer’s instruction and the RNA from the polysomal fractions (≥3 associated ribosomes ...
-
bioRxiv - Bioengineering 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... DNA/protein complexes were then precipitated with anti-CTCF antibody (EMD Millipore) or rabbit IgG conjugated with protein A and G beads ...
-
bioRxiv - Biochemistry 2023Quote: ... The pooled protein was concentrated using a centrifugal concentrator (Millipore, 100 kDa) prior to injection onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... A mixture consisting of rCas9NLS protein (0.66 mg/ml, Sigma-Aldrich CAS9PL), Phenol red (0.05% ...
-
bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were transferred to a PVDF membrane (Millipore, 0.2 μm). The membrane was blocked in 5% nonfat dry milk (Biofroxx ...
-
bioRxiv - Cancer Biology 2024Quote: ... as per manufacturer instructions and 0-5µg/mL OVA protein (Millipore Sigma) for 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
-
bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with Immobolin Forte Western HRP substrate (Sigma, WBLUF0100) and imaged with a ChemiDoc Imaging System (BioRad) ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, #WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
-
bioRxiv - Pathology 2024Quote: ... and the blotted protein bands were revealed by the ECL system (Millipore).
-
bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
-
bioRxiv - Microbiology 2024Quote: ... The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bead-bound proteins were eluted with 200 ng/µL 3ξFLAG peptide (Sigma) by incubating at 4°C for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... and protein was visualized using Immunobilon Western Chemilumiescent HRP Substrate (Millipore, WBKLS0500) on an ImageQuant LAS 4000 (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
-
bioRxiv - Immunology 2023Quote: ... The protein in PBS was 0.22um filtered using spin filters (Millipore Sigma) prior to Size exclusion Chromatography (SEC) ...
-
bioRxiv - Cancer Biology 2024Quote: ... for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125) for LONP1 immunoprecipitation on a rotating platform at 4°C ...