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Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cell culture by RIPA lysis buffer (Sigma Aldrich) with protease and phosphatase inhibitor cocktail and sample reducing agent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-antibody binding was visualized using Immobilon Forte Western HRP Substrate (Millipore) on the LAS-3000 imaging system (FUJIFILM ...
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bioRxiv - Bioengineering 2021Quote: ... filtered through a Millex-HV 0.45-μm low protein-binding membrane (Millipore), and aliquoted ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted from transfected meningeal cells using RIPA buffer (R0278, Sigma) and run on Western blot at 20ug per well ...
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bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Biochemistry 2020Quote: ... The partially purified protein was concentrated (10 kDa cut-off, EMD Millipore) and exchanged into 100 mM potassium phosphate buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was loaded onto a 0.45 μm nitrocellulose filter (HA Millipore), and the filters were washed with 3 ml 30 mM imidazole (pH 7.1) ...
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bioRxiv - Biophysics 2021Quote: Routine chemicals used for protein expression and purifications were purchased from Sigma, Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was recovered and proteins were quantified with Bradford assay (Sigma Aldrich) and samples were stored at -80°C until further used ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred to an Immunobilon-FL Transfer Membrane (Millipore, Bedford, MA) at 110 volts for 50 min in ice cold transfer buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Transferred protein quantities were detected with Ponseau S solution (Sigma-Aldrich, USA). Transferred proteins were blocked with 1% BSA (KPL Detector™ Block ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and protein was eluted with 100 µg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression and purification and enzyme assays were obtained from Sigma-Aldrich, TCI chemicals ...
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bioRxiv - Biochemistry 2021Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37°C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was incubated on ice with dried cardiolipin (Sigma-Aldrich, USA) to a final lipid concentration of 0.1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Protein Kinase A Catalytic Subunit from bovine heart (PKA, EC 2.7.11.11, Sigma) was reconstituted in bi-distilled water containing 6 mg/ml DTT at a concentration of 50 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized protein was then loaded onto 2 mL M2 affinity matrix (Sigma) previously equilibrated with wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... The resolved proteins were then electrically transferred to a PVDF membrane (Millipore). Immunoblotting was probed with indicated antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were preparared using RIPA lysis buffer (Sigma, ST. Louis, USA) supplemented with protease inhibitor cocktail (Complete ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of the isolated proteins was determined using Bradford reagent (Sigma). 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were immobilized on to a reinforced nitrocellulose membrane (Millipore Sigma) with 1× NuPage transfer buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2020Quote: ... Protein G sepharose was used for anti-SOCS1 (Millipore, 04-002, RRID:AB_612104) IP ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma Alrich). Protein lysates (50 μg ...
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bioRxiv - Neuroscience 2021Quote: Proteins from spinal cord tissue were extracted with RIPA buffer (Sigma Aldrich) containing a cocktail of protease inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein was added to 7.8 nmol nitrocefin (Sigma) and absorbance at 495 nm measured kinetically for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: Proteins were purified using HisSelect Sepharose beads (Sigma-Aldrich, St. Louis, USA) and the in vitro kinase reactions were carried out as described earlier (Huesmann et al. ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... The target proteins were collected and desalted using Amicon (10kDa cut) (Millipore) before subjecting to the HiTrap Q column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto a polyvinylidene difluoride membrane (#IPVH00010; Millipore, Bedford, MA). The membrane was blocked and probed with primary and secondary antibodies sequentially ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and the resolved proteins were transferred onto PVDF membranes (Immobilon-P, Millipore) using a semi-dry blotting assembly (Amersham Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein extracts supplemented with β-mercaptoethanol (750 mM final, Sigma-Aldrich) were analysed by western blot ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Cell Biology 2020Quote: ... Then pre-processed protein A agarose (Catalog No. P3476, Sigma, MO, USA) was added to mixture ...
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bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
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bioRxiv - Biophysics 2021Quote: ... Crosslinked proteins were precipitated with 20% (w/v) trichloroacetic acid (TCA, Sigma) on ice for 90 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were visualized by gel staining with Coomassie Brilliant Blue (Sigma Aldrich).
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bioRxiv - Cancer Biology 2021Quote: ... Antibody-reactive proteins were detected by western chemiluminescent HRP substrate (ECL) (Millipore). Images were acquired by FluorChem FC3 image analyzer (Molecular Devices).
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked with 5% skim milk in TBST (10 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was filtered with a 0.22 μm pore size filter (Millipore) prior to size-exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were probed with anti-thymidylate synthase antibody (Millipore, Cat:MAB4130, 1:500), anti-CDK6 antibody (Cell Signalling ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were detected with Luminata Forte Western HRP Substrate (EMD Millipore) or SuperSignal™ West Atto Ultimate Sensitivity Substrate (A38555 ...