Labshake search
Citations for Millipore Sigma :
7851 - 7900 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
-
bioRxiv - Immunology 2023Quote: ... The protein in PBS was 0.22um filtered using spin filters (Millipore Sigma) prior to Size exclusion Chromatography (SEC) ...
-
bioRxiv - Cancer Biology 2024Quote: ... for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125) for LONP1 immunoprecipitation on a rotating platform at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... we precipitated the eluted proteins by adding trichloroacetic acid (#T0699, Sigma Aldrich) to 20% final concentration ...
-
bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
-
bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were to the following proteins: Rat anti-MBP (Millipore, MAB386), Rabbit anti-SMI32 (Abcam ...
-
bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA, #IPFL00010) in NuPAGE transfer buffer at 20V for 60 minutes at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
-
bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
-
bioRxiv - Plant Biology 2024Quote: ... MAPK6 and MKP2 protein were detected with anti-AtMPK3 antibody (Sigma M8318), anti-AtMPK6 antibody (Sigma M7104 ...
-
bioRxiv - Microbiology 2023Quote: ... Following a reduction of proteins with 10 mM DTT (Sigma-Aldrich, Germany) in 100 mM TEAB at 57 °C for 30 min and dehydration of gel pieces ...
-
bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...
-
bioRxiv - Microbiology 2023Quote: ... The elution containing native DnaN protein was concentrated by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2023Quote: Cells for protein extraction were lysed using RIPA lyses buffer (Sigma #R0278) containing protease and phosphatase inhibitor cocktails (cOmplete™ ...
-
bioRxiv - Microbiology 2023Quote: ... Eluted protein was concentrated using spin concentrators (Millipore Amicon Ultra-15 -3K) to ∼1.5-2 mL and subsequently dialyzed in one liter of storage buffer (150 mM NaCl ...
-
bioRxiv - Molecular Biology 2023Quote: ... protein synthesis was inhibited by blocking translation step using cycloheximide (Sigma, #C7698) and MMP activity was inhibited by ilomastat (GM6001) ...
-
bioRxiv - Molecular Biology 2023Quote: ... and protein was extracted in RIPA buffer containing protease inhibitor mixture (Sigma) and phosphatase inhibitor cocktail (Roche) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies to the following proteins were used in this study: 53BP1 (Millipore), γ-H2AX (Ser 139 ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were stained with ReadyBlue Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed on a ChemiDoc Imaging System by Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2023Quote: Total protein was extracted from cell lines using TRI Reagent (Sigma, UK). 40μg of isolated protein samples were then separated using 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
-
bioRxiv - Physiology 2023Quote: ... Final protein concentrations were calculated by Bradford assay (Sigma-Aldrich Canada, Ltd.,) according to manufacturer’s guidelines with bovine serum albumin (BioRad Laboratories ...
-
bioRxiv - Neuroscience 2023Quote: ... total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO). Chemiluminescence was measured in a ChemiDoc Imaging System (Bio-Rad ...
-
bioRxiv - Immunology 2023Quote: ... Proteins were visualized using peroxidase-coupled secondary antibodies (rabbit-POD, Sigma-Aldrich, A6154 ...
-
bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
-
bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
-
bioRxiv - Bioengineering 2024Quote: ... Protein expression was then induced by addition of 1M IPTG (Sigma-Aldrich), before harvesting of cells after 8 hours ...
-
bioRxiv - Cell Biology 2023Quote: ... and protein analysis of tau and β-III-tubulin (Sigma T-8660) by western blot as described above.
-
bioRxiv - Biochemistry 2023Quote: ... Proteins were then transferred to 0.45 µm PVDF membranes (Immobilon-P, Millipore) in a wet tank apparatus ...
-
bioRxiv - Neuroscience 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein was labeled using an atto-488-NHS ester (Sigma Aldrich 41698) and then dialyzed again to remove unconjugated dye ...
-
bioRxiv - Immunology 2023Quote: ... proteins (50 μM) and TCEP (5 mM) reducing agent (Sigma Aldrich, USA) were mixed 1 hour at RT and then left overnight (O/N ...
-
bioRxiv - Microbiology 2024Quote: ... The cleaved protein was concentrated using Amicon 10 KDa MWCO concentrators (Millipore), and further purified by size exclusion chromatography using a Superdex 200 (16/60 ...
-
bioRxiv - Microbiology 2024Quote: ... the separated proteins were transferred onto Immobilon-P PVDF membrane (Merck Millipore). The membrane was blocked with 10% skim milk in Tris Buffered Saline ...
-
bioRxiv - Immunology 2023Quote: Dry protein pellets were dissolved in 100mM TrisHCL (pH 8.5, Sigma T1503) to determine the protein concentration using the Bradford protein assay (Thermo Scientific 23200) ...
-
bioRxiv - Cell Biology 2023Quote: ... 20 µg protein per sample was reduced with 3 mM TCEP (Sigma) and alkylated with 10 mM iodoacetamide (Sigma ...
-
bioRxiv - Molecular Biology 2023Quote: ... The protein was concentrated using Amicon Ultra-4 10 kDa MWCO (Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
-
bioRxiv - Microbiology 2022Quote: The MLB1 CPNTD protein was produced in Rosetta 2 (DE3) cells (Novagen) cultured in 2×YT media with overnight expression at 18 °C induced with 0.4 mM IPTG ...
-
bioRxiv - Developmental Biology 2022Quote: ... Anti-Prosurfactant Protein C (pro-SP-C) antibody (1:4000) (Millipore, #AB3786). Antibodies were incubated in 2% powder milk in TBS pH 7.4 ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were separated using SDS-PAGE and transferred to PVDF membranes (Millipore) for Western blot analysis ...
-
bioRxiv - Plant Biology 2022Quote: ... incubated 40 min with 50 µL of magnetic protein G Dynabeads (Millipore) fused to a GFP monoclonal antibody (1/500 in PBS 0.002 % tween) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and proteins were transferred onto a PVDF membrane (Millipore, Cat. No. ISEQ00010). The membrane was blocked with 5% non-fat milk in tris buffered saline with 0.1% Tween 20 (TBS-T ...
-
bioRxiv - Plant Biology 2022Quote: ... Eluted protein was concentrated with a 30 kDa centrifugal filter unit (Millipore) and purified by size-exclusion chromatography (HiLoad 16/600 Superdex 200 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The eluted proteins were concentrated using an Amicon Ultra centrifugal device (Millipore) with 100 kDa MW cut-off ...
-
bioRxiv - Biochemistry 2022Quote: ... Solubilized protein was loaded onto 1.5 mL of M2 affinity matrix (Sigma) equilibrated with wash buffer (20 mM MES pH 6.0 ...
-
bioRxiv - Biochemistry 2022Quote: ... The concentrated protein was immobilized on cyanogen bromide activated Sepharose 4G (Sigma) according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... the reduced proteins were alkylated with 150 mM Iodoacetamide (Millipore, Bedford, USA) in darkness at room temperature for 30 min ...