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Citations for Millipore Sigma :
8151 - 8200 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tag of the eluted protein was cleaved by thrombin (Sigma), incubated at 4 °C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated to >20 mg/mL on a Centricon device (Millipore).
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bioRxiv - Biochemistry 2023Quote: SYPRO Orange Protein Gel Stain (Cat#S5692, Sigma-Aldrich, St. Louis, USA) were diluted 1:2500 into the assay buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... proteins entrapped in gel bands were reduced with 10 mM dithiothreitol (Sigma) at 60°C for one hour and then alkylated with 50 mM iodoacetamide (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... The eluted protein was concentrated with a 100kDa-cutoff spin concentrator (Millipore), after which 1:10 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... Protein synthesis was inhibited by adding cycloheximide (CHX) (Sigma, catalog number: 01810) at a final concentration of 100 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on a MeOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by semidry blotting for 1 h at 10V ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentration was quantified by Coomassie staining using BSA standards (Sigma P0914).
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bioRxiv - Genomics 2023Quote: ... protein stability was assessed by adding 5 mg/ml cycloheximide (Sigma-Aldrich) to the cell growth medium for the indicated times prior to harvest ...
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bioRxiv - Genetics 2022Quote: ... Eluted proteins were loaded on the centrifugal filter unit (Millipore-Sigma, UFC8030) and centrifuged at 3,500 g 4°C for 20 minutes to eliminate any remaining maltose from the elute ...
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bioRxiv - Biochemistry 2022Quote: ... protein expression was induced with 1 μg/mL doxycycline hyclate (Sigma Aldrich), and the culture was placed in a shaking incubator maintaining 37°C and a 5% CO2 atmosphere ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was transferred to a low background fluorescence PVDF membrane (Millipore Sigma) and the membrane was blocked in 5% nonfat milk in 1x TBST ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were transferred on an EtOH-activated polyvinylidene fluoride (PVDF) membrane (Millipore) by wet blotting overnight at 40 mA and 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were directly labeled using alkaline phosphatase-coupled streptavidin (Sigma-Aldrich). The blots were developed in a solution of nitroblue tetrazolium chloride (NBT ...
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bioRxiv - Cell Biology 2022Quote: ... The protein bands were visualized by an ECL detection system (EMD Millipore).
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... Cell lysates were incubated with Protein G sepharose beads (Sigma-Aldrich P3296) with anti-RpoC antibody (Biolegend clone NT73 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then filtered through a 10kDa protein filter (MRCPRT010, Sigma Aldrich) via centrifugation at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... The dialyzed protein was concentrated using a 30 kDa MWCO concentrator (Millipore) and was further purified by size-exclusion chromatography on a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Genetics 2023Quote: ... the Cas9 protein was expressed in BL21 Rosetta Escherichia coli strain (Novagen) in magic medium at 37 °C for 12 h followed by 24 h at 18 °C ...
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bioRxiv - Cell Biology 2023Quote: ... protein inhibitor cocktail (1:100) and Benzonase nuclease (Novagen, 70746, 1:100) in PBS ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Immunology 2023Quote: ... proteins were transferred to a hydrophilic PVDF membrane (Imobilon E, Millipore-Sigma). Membranes were blocked with TBS containing 1 % casein and 2 % BSA followed by incubation with primary antibodies (survivin 1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich). Prior to their use ...
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bioRxiv - Systems Biology 2023Quote: ... We then transferred proteins to a low background PVDF membrane (Millipore, IPFL00010) for 15h at 15V using the TRIS-Glycine buffer (48 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... proteins were electrotransferred to a polyvinylidene difluoride membrane (Millipore, Burlington, MA, USA) using a semi-dry transfer blotter (Atto) ...
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bioRxiv - Cell Biology 2023Quote: ... Bound proteins were eluted in Flag peptide (Sigma, F4799, 150 ng/μl) for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: Pure proteins were concentrated using 10 kDa Amicon Ultra membrane concentrators (Millipore), flash-frozen in liquid nitrogen and stored at −80℃.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using the Bicinchoninic acid assay (BCA, Sigma-Aldrich). Proteins were then denaturated with Laemmli Buffer (Tris pH 6.8 (200mM) ...
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bioRxiv - Neuroscience 2023Quote: ... transferrin and transthyretin) using the ProteoPrep 20 plasma protein immunodepletion kit (Sigma), by performing two immunodepletion cycles ...
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bioRxiv - Biochemistry 2023Quote: ... Both the proteins were concentrated in a 50,000 MWCO Amicon Concentrator (Millipore) to <1 mL and injected onto a Superdex™ 200 10/300 GL Increase size-exclusion column (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... total protein was visualized using PonceauS staining (Sigma-Aldrich, St. Louis, MO), blocked using 5% non-fat milk powder in tris-buffered saline with tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Eluted protein was then concentrated using 3K-50K MWCO Amicon concentrators (Millipore). All proteins were aliquoted in PCR tubes ...
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bioRxiv - Microbiology 2023Quote: ... 50 µg protein per sample were reduced with 10 mM DTT (Sigma) for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Millipore, Billerica, USA), which was then blocked with 5% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were precipitated with 1:4 trichloroacetic acid solution (TCA, Sigma Aldrich) and washed with acetone ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were to the following proteins: Rat anti-MBP (Millipore, MAB386), Rabbit anti-SMI32 (Abcam ...
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bioRxiv - Cancer Biology 2024Quote: mTOR kinase domain protein was purchased from Sigma-Aldrich (Cat # 14-770). ETV7 protein (Cat # TP307742 ...
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bioRxiv - Physiology 2024Quote: ... Proteins were then transferred to PVDF membranes (Millipore, Burlington, MA, USA, #IPFL00010) in NuPAGE transfer buffer at 20V for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were then transferred onto Immobilon-P PVDF membranes (#IPVH00010; Millipore) for immunoblotting ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Plant Biology 2024Quote: ... MAPK6 and MKP2 protein were detected with anti-AtMPK3 antibody (Sigma M8318), anti-AtMPK6 antibody (Sigma M7104 ...
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bioRxiv - Microbiology 2023Quote: ... Following a reduction of proteins with 10 mM DTT (Sigma-Aldrich, Germany) in 100 mM TEAB at 57 °C for 30 min and dehydration of gel pieces ...
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bioRxiv - Molecular Biology 2023Quote: ... Transferred proteins were stained in Ponceau S (0.1% Ponceau S (Sigma-Aldrich), 5% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... The elution containing native DnaN protein was concentrated by ultrafiltration (Millipore, UFC901096) and maintained in 20 mM Tris-HCl ...