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Citations for Millipore Sigma :
7951 - 8000 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 20 µl Magna ChIP™ Protein A Magnetic Beads (Sigma, 16-661) and antibodies (1 µg per 1 million-cell chromatin ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of protein samples were supplemented with β-mercaptoethanol (Sigma; M6250) and diluted in 1X Laemmli Sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Eluted protein was concentrated using spin concentrators (Millipore Amicon Ultra-15 -3K) to ∼1.5-2 mL and subsequently dialyzed in one liter of storage buffer (150 mM NaCl ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were blocked with 5 % skimmed milk in TTBS buffer (10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... MBP fusion proteins were expressed in Escherichia coli Rosetta (DE3) pLysS (Novagen) and purified using Amylose Resin (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... The digested protein samples were then concentrated with an Amicon Ultra (Millipore) concentrator (50 kDa MWCO ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein was transferred to 0.45μm PVDF membranes (Millipore Sigma, Cat#IPVH00010) with transfer buffer (formula ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were transferred onto a 0.45-μm polyvinylidene difluoride membrane (Millipore). Next ...
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bioRxiv - Cell Biology 2024Quote: Cellular protein was collected in RIPA buffer (R0278, Millipore Sigma, Burlington, MA) containing ReadyShield® Protease and Phosphatase Inhibitor Cocktail (PPC- 2020 ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... 50 µL of agarose beads coupled to protein A (Millipore 16–157) or G (Millipore 16–201 ...
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bioRxiv - Immunology 2024Quote: ... were coupled to 30 μl Protein G Sepharose™ beads (#P3296, Millipore) by 2 h incubation at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to polyvinylidene difluoride (PVDF) membranes (Immobilon-P, Millipore, IPVH100010). Membranes were blocked for at least 1 h with 5% non-fat dry milk and 3% BSA in TBS-T (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Biochemistry 2024Quote: ... Protein-containing factions were concentrated with Amicon Ultra Filter Devices (Millipore, USA) 10 kDa cutoff ...
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bioRxiv - Biochemistry 2024Quote: ... The separated proteins were then transferred to a PVDF membrane (Millipore, IPVH00010) and immunodetection was carried out using antibodies against the target proteins ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using anti-FLAG (Sigma-Aldrich, ST. Louis, MO, USA) and HRP-labeled anti-mouse IgG (Cytiva ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were then blocked and probed with anti-Drp1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Exogenous protein expression was induced 24h after transfection by adding Doxycycline (Sigma) to the media at a concentration of 50 ng/ml and induction was performed for 24h ...
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bioRxiv - Genetics 2024Quote: ... Protein was transferred to a methanol-treated polyvinylidene difluoride membrane (Millipore Sigma) using a Mini Trans Blot Electrophoretic Transfer Cell (Biorad ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using a BCA assay kit (#QPBCA, Sigma-Aldrich). Protein samples were mixed with DTT (#D9779-10G ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations in the collected fractions were measured using Bradford reagent (Sigma).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Proteins in the SDS gels were transferred to PVDF (Cat# IPVH00010, Millipore) membrane using an electroblotting apparatus (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... Eluted protein was concentrated using Amicon Ultra 10K spin columns (Sigma-Aldrich) and dialyzed against hSSB storage buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Bioengineering 2024Quote: ... the protein was concentrated using Amicon centrifugal filters (Millipore, Burlington, MA, USA) with a molecular weight cut off of 10 kDa.
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bioRxiv - Cell Biology 2024Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Pathology 2024Quote: ... Proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck Millipore), blocked with 5% non-fat dry milk ...
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bioRxiv - Plant Biology 2024Quote: ... After transferring proteins from gels to polyvinylidene fluoride (PVDF) membranes (Merck-Millipore), membranes were probed with horseradish peroxidase (HRP)-conjugated FLAG antibody (A5892 ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein within gel was transferred to 0.45 μm-PVDF membrane (Millipore) at a constant 300mA current for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein expression was induced by adding 0.6mM – 0.8mM of IPTG (Sigma) to the cell culture and shaking at 18°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... 15 ug of total proteins were reduced with 5 mM dithiothreitol (Sigma) for 1 h at room temperature and alkylated with 10 mM iodoacetamide (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... the protein was incubated with 2 units of bovine thrombin (Sigma Aldrich) in the same dialysis buffer as above ...
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bioRxiv - Microbiology 2024Quote: ... in the presence of protein A-sepharose beads (1/20 µl, Sigma). Immune complexes eluted were separated on 4-20% SDS PAGE gels (mini protean TGX ...
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bioRxiv - Microbiology 2024Quote: ... and then the proteins were transferred to PVDF membranes (Millipore, MA, USA) using a semi-dry transmembrane apparatus ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated by using Amicon Ultra 50K device (Merck Millipore) and stored in elution Buffer II (20 mM Tris-HCl [pH 8] ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and the separated proteins were transferred onto nitrocellulose membranes (NC, Millipore, USA) in ice-water ...
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bioRxiv - Neuroscience 2024Quote: ... or the Coomassie Protein Assay reagent (Sigma-Aldrich, St Louis, MO, USA). Quantification of Aβ1-40 and Aβ1-42 in soluble and insoluble fractions was performed using the EZHS-SET ELISA kit ...
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bioRxiv - Neuroscience 2024Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were detected using chemiluminescence and Immobilon Western HRP Substrat (Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Synthetic Biology 2024Quote: ... Proteins including ovalbumin (OVA) and OVA-AF488 were purchased from Sigma (#A2512) and ThermoFisher (O34781 ...
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bioRxiv - Immunology 2024Quote: ... The purified protein was concentrated by an Amicon spin filter (Millipore Sigma) and filtered by a 0.22 mm centrifuge Tube Filter (Costar) ...
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bioRxiv - Immunology 2024Quote: ... protein detection was carried out with antibodies against MAFB (HPA005653, Sigma Aldrich), total GSK3α (#4337 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Millipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... Proteins from gels were transferred on to polyvinylidene difluoride membrane (Millipore, #IPVH00010) at 30 V overnight ...
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bioRxiv - Cell Biology 2024Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in PBS and incubated at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in each sample was measured using BCA assay (Sigma-Aldrich). 30µg of each sample were then prepared using the iST-NHS kit (Preomics) ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... was dissolved into 5 mL of BugBuster™ protein extraction reagent (Sigma). Using a sterile loop ...
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bioRxiv - Cell Biology 2024Quote: ... Ice-cold protein extraction lysis buffer was composed of RIPA (Sigma, R0278), Protease inhibitor cocktail (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... and proteins were concentrated using an Amicon ® Ultra Centrifugal Filter (Millipore) with a 30 kDa cutoff ...