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Citations for Millipore Sigma :
7801 - 7850 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 25μg protein lysate were resuspended in Laemmli buffer 1X (Sigma, S3401-1VL), denatured at 95°C for 5 minutes and loaded onto a polyacrylamide gel (Mini-PROTEAN(R ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was qualified by Bradford assays (Sigma-Aldrich, B6916-500 mL). 30 μg soluble proteins were resolved on a 15% sodium dodecyl sulphate poly acrylamide gel via electrophoresis (SDS-PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein pellet was washed with −20°C cold acetone (Sigma-Aldrich), vortexed ...
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bioRxiv - Microbiology 2023Quote: ... the protein-DNA complexes were immunoprecipitated with an anti-FLAG antibody (Sigma) and Protein A sepharose beads ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Separated proteins were then electroblotted onto polyvinylidene difluoride (PVDF) membranes (Millipore, USA). Following the transfer ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...
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bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504) before being scanned on a flatbed scanner (Epson V800 Photo) ...
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Protein concentration was measured using a Bradford assay (Sigma France, Lezennes, France). For SDS-PAGE analysis of purified proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore) overnight at 4°C and blocked in either 5% nonfat powdered milk or 5% bovine serum albumin in TBS with Tween 20 (pH 7.6 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... protein samples were prepared with sample buffer that contains 10% glycerol (Sigma) and 5% 2-mercaptoethanol (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore). Digested glycans were analyzed by HILIC-UPLC as before (51 ...
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bioRxiv - Microbiology 2023Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Millipore, IPVH00010). The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated to approximately 3 mg/ml by ultrafiltration (Millipore, UFC905096) at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Purified protein was then concentrated to 40mg/mL by centrifugal concentration (Millipore).
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bioRxiv - Cell Biology 2023Quote: ... and 4 g digitonin:1 g mitochondrial protein (Sigma-Aldrich, #D141-100MG). Mitochondria were incubated on ice for 20 minutes and then centrifuged at 20,000 x g for 10 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Protein bands were detected by Luminol-based enhanced chemiluminescence (EMD Millipore WBKLS0500). Immunoblots were captured on film and quantified using Image J.
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bioRxiv - Neuroscience 2022Quote: ... Proteins were visualized using chemiluminescence (Immobilon Western Chemiluminescent HRP Substrate, Millipore, #WBKLS0500) on Bio-Rad ChemiDoc (XRS+) ...
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bioRxiv - Neuroscience 2022Quote: ... and the protein concentration determined with a BCA assay (Sigma, Deisenhofen, Germany). Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... Transferring the protein from the gel to the PVDF membrane (Millipore #ISEQ00010). Membranes were blocked with 5% milk for one hour at room temperature and incubated with appropriate dilutions of primary antibody overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of 0.5 mg/mL OVA protein (Sigma) in cRPMI at 37℃ in 5% CO2 incubator for 72 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were detected with anti-Flag antibody (Sigma, F1804, monoclonal, 1:1,000) or anti-Sso antibody (rabbit antiserum ...
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bioRxiv - Cell Biology 2022Quote: ... The protein complex was concentrated using centrifuge filters (Amicon Ultracel-30K, Millipore) and subjected to SEC using a Superdex 200 column (HiLoad 16/600 Superdex 200 pg ...
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bioRxiv - Physiology 2022Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: ... and then filtered through a 0.45-μm low protein binding membrane (Millipore). HEK293T cells were seeded into 12-well plates at approximately 40-50% confluency and 50 μl filtered virus-containing supernatant was added to the 12-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... the expression of GST fusion proteins was induced with isopropylthiogalactoside (IPTG, Sigma) at 16 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were then transferred to 3 kDa protein-filter columns (Sigma-Aldrich) and centrifuged at 12,000g for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Optimal protein expression and solubility was achieved in RosettaTM(DE3) pLysS (Novagen) for wild-type His::PfPKG ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended cells were supplemented with Bug Buster protein reagent (Millipore #70584-3) before being sonicated ...
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bioRxiv - Biochemistry 2024Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Bioengineering 2024Quote: ... One hundred µL of protein samples or lysostaphin standards (SIGMA-L9043-5MG) diluted in 0.01% Tween 80 (SIGMA-P5188-100ML ...
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bioRxiv - Cancer Biology 2024Quote: ... equal amounts of protein were run and transferred onto nitrocellulose membranes (Millipore). The membranes were blocked with 5 % milk and incubated with the following antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated proteins were transferred to Immobilon FL polyvinylidene difluoride (PVDF) membrane (Millipore) for immunoblotting ...
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bioRxiv - Microbiology 2024Quote: ... RNA and proteins were extracted from the fractions using Trizol reagent (Sigma) as per manufacturer’s instruction and the RNA from the polysomal fractions (≥3 associated ribosomes ...
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bioRxiv - Bioengineering 2023Quote: ... The proteins were transferred to polyvinylidene fluoride (PVDF) membrane (Millipore, Cat# ISEQ00010), and blocked with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... DNA/protein complexes were then precipitated with anti-CTCF antibody (EMD Millipore) or rabbit IgG conjugated with protein A and G beads ...
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bioRxiv - Biochemistry 2023Quote: ... The pooled protein was concentrated using a centrifugal concentrator (Millipore, 100 kDa) prior to injection onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... A mixture consisting of rCas9NLS protein (0.66 mg/ml, Sigma-Aldrich CAS9PL), Phenol red (0.05% ...
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bioRxiv - Cancer Biology 2024Quote: ... as per manufacturer instructions and 0-5µg/mL OVA protein (Millipore Sigma) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
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bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with Immobolin Forte Western HRP substrate (Sigma, WBLUF0100) and imaged with a ChemiDoc Imaging System (BioRad) ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, #WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
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bioRxiv - Pathology 2024Quote: ... and the blotted protein bands were revealed by the ECL system (Millipore).
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bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
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bioRxiv - Microbiology 2024Quote: ... The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich) ...