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Citations for Millipore Sigma :
751 - 800 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... for 1 hr at RT and subsequently incubated with primary antibodies: pan-GPR56 (clone H11) (Millipore MABN310, 1:150) and HA-Tag (3F10 ...
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bioRxiv - Microbiology 2020Quote: ... to each sample was added 150 μl TEA buffer containing 0.2 % Triton X-100 and 4 mM mPEG-Mal (10 kDa; Sigma) for a final concentration of 1 mM mPEG-Mal ...
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bioRxiv - Plant Biology 2021Quote: ... The 50 µl pre-washed with washing buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0,1% Tween-20) M2 anti-FLAG magnetic beads (Sigma) was added to supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 100 μL of buffer (50 mM Tris, 150 mM NaCl, 1mM EDTA, pH 7.4) with Flag peptide (Sigma) and rocked overnight at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Purified MAYV bands were collected and concentrated using a 150 KDa cut-off filter (Amicon Ultra-2 100Kd, Millipore). Final purified MAYV samples were stocked at −80°C until use and quantified using plaque assays ...
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bioRxiv - Microbiology 2020Quote: Biofilms were thoroughly air-dried and stained with 150 μL of 0.2% crystal violet (w/v) (Sigma-Aldrich, USA) supplemented with 1.9% ethanol (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 0.03% NP40) to a final KCl concentration of 150 mM and treated by 500 U of benzonase (Sigma, E1014) for 30 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... In both experiments 4 dpf larvae were exposed to 150 μg/ml LPS (B11.04; Sigma Aldrich, Zwijndrecht, the Netherlands) in E2 medium or control E2 medium for 30 minutes in Petri dishes (n=50 in 25 ml ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed with TNE buffer (Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA) supplemented with 1% triton X-100 (Sigma), protease inhibitors (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... which was pretreated with 0.01% poly-L-lysine (mol. wt. 150 K–300 K; Millipore Sigma, St. Louis, MO) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently scraped with ice cold RIPA buffer (50 mM Tris pH 8, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP-40; all chemicals from Sigma Aldrich) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Pathology 2022Quote: ... Samples of a minimum of 105 peripheric white blood cells or splenocytes were mixed with 150 µl of lysis solution (154 mM NH4Cl, 10 mM KHCO3 and 0.082 mM EDTA (Sigma)) for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were harvested at 1,000 g for 10 min and subsequently resuspended either in 1 ml ice cold IPP150 buffer (10 mM Tris-HCl pH 6.8, 150 mM NaCl, 0.1% IGEPAL CA-630; Sigma I8896) or in CHAPS buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... PVDF membranes were then blocked in 5% skim milk in TBS-Tween (Tris-buffered saline (50 mM Tris, 150 mM NaCl, pH = 7.6) mixed with 0.1% Tween 20 (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2020Quote: ... Cell pellets were resuspended in Buffer A (25 mM Tris-HCl pH 8.5, 150 mM KCl, 50 mM Imidazole, 10% glycerol) (Sigma) with 25 mM Magnesium chloride ...
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bioRxiv - Bioengineering 2020Quote: ... were dissolved in 150 g of 1.6M HCl solution and stirred with 8.6 g of tetraethyl orthosilicate (TEOS, 98%, Sigma-Aldrich) at 40 °C for 20 h ...
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bioRxiv - Cell Biology 2021Quote: ... remaining tissue was then minced and digested by Collagenase IV (0.1 g/100 mL, sigma) and DNase I (150 U/mL, Sigma) for 40 min at 37°C whilst being gently shaken ...
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bioRxiv - Immunology 2020Quote: ... The resulting membrane pellet was resuspended in ice cold 500 μl TNE buffer (10 mM Tris/Cl [pH 7.4], 150 mM NaCl, 5 mM EDTA, 1% Triton X-100 [Sigma] ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were washed with cold PBS and incubated for 15 minutes at 4°C with RIPA lysis buffer (30 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10% glycerol, 1% Triton X-100 (Sigma) supplemented with a 1 mM NaF ...
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bioRxiv - Biochemistry 2020Quote: ... Yta7 and mutant versions were eluted in 1 CV of lysis buffer containing 150 mM KCl with 0.5 mg/ml 3x FLAG peptide (Sigma), followed by 2 CV of lysis buffer containing 150 mM KCl with 0.25 mg/ml 3x FLAG peptide ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 20 ml Buffer A (50 mM HEPES, 150 mM NaCl, 1 mM DTT, pH 7.5) supplemented with SigmaFast protease inhibitor (Sigma). Cells were lysed by sonication and insoluble debris were removed by ultracentrifugation at 100,000g for 20 mins ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 µl of cell suspension was manually aspirated into the cell chamber pre-filled with mineral oil (Sigma-Aldrich). A fourth 2.5 ml glass syringe (SGE ...
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bioRxiv - Microbiology 2020Quote: ... Hydrophilic interaction liquid chromatography was performed at 0.2 ml/min on a ZIC-pHILIC column (2.1 mm × 150 mm, EMD Millipore) at 45 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Neurospheres from each condition were lysed in buffer containing 0.1% triton X-100 in 50 mM Tris-HCl and 150 mM NaCl and Protease Inhibitor Cocktail (Sigma). Samples were then sonicated and subsequently spun down at 13,000 RCF for 10 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl, 1 % NP-40, 0.5 % sodium deoxycholate, and 0.1 % SDS, supplemented with protease inhibitor cocktail; Sigma-Aldrich) for 30 min at 4°C and the protein content was measured by DC Protein Assay (Bio-Rad) ...
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bioRxiv - Plant Biology 2020Quote: ... Leaf tissue was ground in extraction buffer (150 mM NaCl, 50 mM Tris [pH 7.5], 0.2 % Nonidet P-40 [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2021Quote: ... The MIDAS screening pools were prepared in LC-MS grade 150 mM ammonium acetate pH 7.4 (Sigma-Aldrich 73594) and pH-adjusted with ammonium hydroxide (Sigma-Aldrich 338818) ...
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bioRxiv - Neuroscience 2021Quote: ... AAV vectors were produced by seeding HEK293T cells (4.5×106 cells per dish) on 90-150 Ø15cm tissue culture dishes (Sigma). Two days later ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM DTT), then incubated with cleavage buffer (20 mM Tris-HCl [pH 8.0], 150 mM NaCl) containing thrombin (Sigma) overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 10% glycerol), then incubated with cleavage buffer (20 mM Tris-HCl [pH 8.0], 150 mM NaCl) containing thrombin (Sigma) overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of TBS buffer (20 mM Tris.HCl pH 8.0, 150 mM NaCl) containing 25 mM sodium cholate (Sigma-Aldrich) was added to the dried lipid film ...
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bioRxiv - Neuroscience 2021Quote: ... 3X antibody stock solutions were prepared containing 0.075 ng/μl 2B7-Tb and 0.75 ng/μl MW1-D2 in TR-FRET antibody buffer (10mM Tris pH 7.4, 150 mM NaCl, 0.25% Igepal, 0.1% Bovine Serum Albumine (BSA, Sigma, #A7030-500G)) ...
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bioRxiv - Physiology 2021Quote: ... resuspended in buffer S (50-mM Tris, pH 8, 150-mM NaCl, 25-mM imidazole, 0.5% CHAPS, and 0.25% Tween 20; Sigma-Aldrich) and lysed with a sonicator ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:200-30000 in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.05% Tween 20 (Sigma, P9416)) plus 5% dry milk (Apex ...
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bioRxiv - Biophysics 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Cell Biology 2021Quote: ... cells were harvested in IP buffer (0.5% Triton X-100, 50 mM Tris-HCl pH 7.5, 150 mM NaCl, protease inhibitor cocktail (Sigma-Aldrich)) and the lysates were cleared by centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... 37°C pre-culture was undertaken before platting 150 ul of pre-culture on LB/Chloramphenicol (Chlo – Sigma Aldrich)/Kanamycin (Kan - Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Purified Vag8 was concentrated and the buffer was exchanged to buffer F (50 mM Tris-HCl pH 8.0, 150 mM NaCl) by ultrafiltration (Amicon Ultra, Merck-Millipore).
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bioRxiv - Microbiology 2020Quote: ... seawater samples spanning six major depths (10, 100, 120, 135, 150, and 200 m) were collected and filtered onto 0.22 μm Sterivex (Millipore) filters ...
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bioRxiv - Molecular Biology 2020Quote: ... were lysed with RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% sodium deoxycholate, 1% Triton X-100, 0.1% SDS, 0.1% P8340 Sigma protease inhibitor). After incubation for 10 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 1 mL of cultures were centrifuged and the cell pellet suspended in 150 μL of Bug Buster (EMD Millipore), supplemented with lysozyme and Benzonase® nuclease as described above ...
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bioRxiv - Genomics 2021Quote: ... The cell pellet was washed twice with 1 ml Wash Buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine (Sigma-Aldrich), 1× Protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2021Quote: 293T cells expressing GST-tagged constructs were lysed on ice in Pulldown Lysis buffer (150 mM NaCl, 50 mM Tris-Cl pH 7.4, 1% NP-40) supplemented with protease inhibitor (HALT, Sigma) cocktail ...
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bioRxiv - Cancer Biology 2020Quote: ... metabolites were resuspended in 100 μL LC-MS grade water and run on a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm, Millipore-Sigma) on a Dionex UltiMate 3000 UPLC using a 0 to 80% gradient of 20 mM ammonium carbonate and 0.1% ammonium hydroxide ...
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bioRxiv - Cell Biology 2020Quote: ... Cell pellets were lysed in NP-40 lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.5% (v/v) IGEPAL CA-630 (Sigma-Aldrich), 1 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... were used for LC-MS analysis along with SeQuant ZIC-HILIC column (150 mm×2.1 mm, 3.5 μm, Merck Millipore, 150442) in the positive mode and SeQuant ZIC-pHILIC column (150 mm×2.1 mm ...
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Sample pellets were diluted in 150-300 μl of 1× phosphate-buffered saline with protease inhibitor cocktail (Sigma-Aldrich) and sonicated ...
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bioRxiv - Molecular Biology 2023Quote: ... to 100 μl of cell lysates which were diluted in 0.25% triton lysis buffer (150 mM NaCl, 10 mM HEPES, pH 7.5, 0.25% Triton X-100, phosphatase inhibitor (Sigma, P5726) and protease inhibitor cocktail (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... 150 µL of culture were added to each well and covered with 50 µL of mineral oil (Sigma-Aldrich) to prevent evaporation ...