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Citations for Millipore Sigma :
1001 - 1050 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Glycoaldehyde phosphate barium salt was synthesized in an aqueous 150 mL reaction by partially dissolving 12 mmol (2.33 g) glycerol phosphate magnesium salt hydrate (Sigma #17766) and 11 mmol sodium meta-periodate (2.35 g) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... All membranes were incubated with the indicated primary antibodies diluted in TBS-T (20mM Tris pH 7.5, 150 mM NaCl, 0.1% Tween-20) with 5% bovine serum albumin (Sigma-Aldrich, A7906) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was injected into a ZIC-pHILIC 150 x 2.1 mm (5 um particle size) column (EMD Millipore). operated on Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed with TBS (50mM Tris pH 7.6, 150 mM NaCl) and blocked with 5% skim milk powder (Sigma Aldrich) in TBST (TBS with 0.05% Tween20 ...
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bioRxiv - Neuroscience 2024Quote: ... For the LC-MS analysis of amino acids 50 µL of the available 150 µL extract were mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.15 M KCl TRAP wash buffer (10 mM HEPES-KOH at pH 7.4, 5 mM MgCl2, 150 mM KCl, 1% NP-40, supplemented with 100 µg/mL cycloheximide [Millipore Sigma C7698-1g] in methanol ...
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bioRxiv - Neuroscience 2024Quote: Mice were terminally anesthetised with an intraperitoneal injection of sodium pentobarbitone (150 mg/kg body weight) and were transcardially perfused with 4% (w/v) paraformaldehyde (PFA; Sigma) in phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in 0.4 µm or 3.0 µm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3– 5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell insert for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... After transfer the blots were blocked in a 5 %(w/v) solution of milk powder in TBS-T (50 mM Tris, 150 mM NaCl, 0.05% Tween 20 (Sigma, P9416)) for 1h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant (5 μl) was injected onto a ZIC-pHILIC 150 × 2.1 mm (5-μm particle size) column (EMD Millipore) connected to a Thermo Vanquish ultrahigh-pressure liquid chromatography (UPLC ...
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bioRxiv - Cell Biology 2023Quote: ... the cover slips were incubated with 150 μL of primary antibody (1:50 anti-FLAG M2 antibody, Sigma-Aldrich F1804) diluted in blocking solution overnight at 4°C ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: Cell lysis was performed using RIPA lysis buffer (50 mM Tris-HCl [Carl Roth, 9090.3], pH 8, 150 mM sodium chloride, 1% IGEPAL CA-630/NP-40 [Sigma-Aldrich, 56741] ...
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bioRxiv - Neuroscience 2024Quote: MEAs and 48-well plates were coated with 400 µl and 150 µl 0.1 mg/ml poly-D-lysine (PDL) (P6407, Sigma-Aldrich), respectively in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Polar Samples were chromatographically separated by injecting a sample volume of 1 µL into a SeQuant ZIC-pHILIC Polymeric column (2.1 x 150 mm 5 mM, EMD Millipore). The flow rate was set to 150 mL/min ...
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bioRxiv - Microbiology 2023Quote: ... Fractions corresponding to ∼150 kDa were pooled and concentrated using a centrifugal filter unit with a 30 kDa molecular weight cut-off (Millipore) via centrifugation at 4000 ξ g and 4 °C for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Reh1-bound ribosomes were eluted in 100 ul of IP buffer supplemented with 0.1% Igepal and 150 ug/ml 3xFLAG peptide (Sigma Milipore) for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in 0.4 μm or 3.0 μm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3–5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell inserts as we previously described41 ...
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Cell Biology 2023Quote: ... non-fat milk dissolved in Tris-buffered saline (TBS, 50 mM Tris-HCl, 150 mM NaCl) with 0.1% (v/v) Tween-20 (Sigma-Aldrich) (0.1% TBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Colloidal probe cantilevers were immersed for 60 minutes in Tris-buffered saline (TBS; Tris, 50 mM; NaCl, 150 mM; pH 8.5) containing 4 mg mL-1 of dopamine hydrochloride (Sigma-Aldrich), rinsed in TBS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and the supernatant concentrated down to 150–200[μl for each sample using 30[kDa Amicon centrifugal filter unit (Millipore). The remaining supernatant was mixed with 13x its volume of binding buffer and DNA was purified with MinElute columns (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL of each sample was injected onto a ZIC-pHILIC 2.1 3 150 mm (5 mm particle size) column (EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Immunology 2024Quote: ... An equal volume of IgM antibody at a concentration of 150-200µg/mL was mixed with equal volume of human IgG obtained from blood (Sigma-Aldrich) at a concentration of 1mg/mL (ratio ∼1:5 ...
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bioRxiv - Systems Biology 2024Quote: ... Reporter cell lines (100-150 million cells) were transduced with packaged retrovirus in the presence of 6 µg/ml polybrene (Sigma) and at low transduction efficiency (< 20% ...
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bioRxiv - Genomics 2022Quote: MB135-iDUX4 myoblasts were treated with or without doxycycline for 14 h and then trypsinized prior to lysis on ice in 1 mL of Lysis Buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40) containing protease inhibitors (Sigma Aldrich). Lysates were precleared using Protein G Sepharose (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... contained the SurA-OmpA under these conditions and so this was concentrated and to <500 μl and loaded to a S200 30/150 SEC column (Sigma). Fractions from the peaks corresponding to SurA-OmpA complexes were pooled and concentrated.
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bioRxiv - Cell Biology 2022Quote: ... completed with 10% heat-inactivated FBS (Thermo Cat. 16000044) in five 150 mm tissue culture dishes (Millipore Sigma Cat. CLS430599) and cultured for three days at 370C in humidified tissue culture incubator with 5% CO2 supply to reach 80% confluency ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with 150 μl of 1% trifluoroacetic acid (TFA) and desalted on poly(styrenedivinylbenzene)-reversed phase support (SDB-RPS) micro-columns (Sigma) as described previously(Humphrey et al. ...
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bioRxiv - Genetics 2022Quote: In vitro differentiated 18 d erythroid cells stained with Hoechst 33342 were resuspended with 150 μl DPBS for analysis with Imagestream X Mark II (Merck Millipore). Well-focused Hoechst-negative single cells were gated for circularity analysis with IDEAS software ...
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bioRxiv - Plant Biology 2022Quote: ... frozen mature tissue was ground into a powder in liquid nitrogen and suspended with extraction buffer (EB: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 × cOmplete™ ULTRA protease inhibitors (Sigma), 1 mM PMSF ...
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bioRxiv - Microbiology 2022Quote: ... series of 6 samples were prepared by pipetting 200 μL of inactivated bacterial cell suspension prepared into 1.5 mL Eppendorf tubes filled with approximately 70 μL of 150-212 μm glass beads (Sigma-Aldrich). 50 μL of a trypsin solution (Porcine pancreas grade ...
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bioRxiv - Neuroscience 2022Quote: ... 150 mM Tris-HCL (pH 8.3) and 150 mM NaCl supplemented with a protease inhibitor cocktail (catalog number 11836170001, Sigma-Aldrich) and phosSTOP phosphatase inhibitor cocktail (catalog number 4906845001 ...
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bioRxiv - Neuroscience 2023Quote: ... Ltk-/- and DKO mice were labeled in vivo by intraperitoneal injections with BrdU (150 µg/g body weight, Sigma-Aldrich,). In some experiments PPP (20 mg/kg body weight ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted by mixing 150-200 mg of plant tissue ground under liquid nitrogen with 1 ml of TRI reagent (Sigma). 200 μl of chloroform was added and after vortexing ...
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bioRxiv - Physiology 2023Quote: ... Oxygen levels were recorded for 3 min intervals at baseline (5 mM glutamate/2.5 mM malate), maximal capacity synthesis ATP (150 μM, ADP) and non-ATP-associated (1 μM Oligomycin, 75351, Sigma-Aldrich) conditions ...
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bioRxiv - Biochemistry 2022Quote: ... 150 mM potassium acetate and 5 mM magnesium acetate) plus a cocktail of protease inhibitors (Sigma-Aldrich, St. Louis, MO). Cells were homogenized by 10 passages through a 22G needle ...
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bioRxiv - Cancer Biology 2023Quote: Polar metabolites were separated on a SeQuant® ZIC®-pHILIC 5µm polymer (150 mm × 2.1 mm) column (EMD Millipore) connected to a Thermo Vanquish ultrahigh-pressure liquid chromatography coupled to a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl,1 mM EDTA and NP40 1% v/v) supplemented with protease inhibitor cocktail l% v/v (Sigma) and quantified by Bradford assay ...
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bioRxiv - Cell Biology 2023Quote: ... cells were scraped into ice-cold RIPA buffer (150 mM NaCl, 50 mM Tris–HCl, pH 7.4, 1 mM EDTA, 1% Triton X-100 (Sigma, T8787), 0.1% SDS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... MSK-QRESS-KIT] and 1 uL was injected into a ZIC-pHILIC 150 x 2.1 mm (5 um particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested with 800µl NP-40 buffer (20mM Tris pH=8, 150-200mM NaCl, 0.5mM EDTA, 1% NP-40, 1mM DTT, 5% glycerol and protease inhibitor cocktail; Sigma-Aldrich). Protease inhibitor cocktail (Bimake ...
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bioRxiv - Molecular Biology 2023Quote: A frozen untreated cell pellet (150 mg wet cell mass) was resuspended in fresh PBS to 150 mg/ml with 0.3 mg/ml lysozyme (Sigma Aldrich) and incubated for 30 min at 37°C in a water bath ...
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bioRxiv - Immunology 2022Quote: K18-hACE2 mice were treated by oral gavage with nirmatrelvir at 150 mg/kg or vehicle (0,5% Methylcellulose (Methocel A4M, Sigma #94378), 2% Tween80 (Sigma #8170611000 ...
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bioRxiv - Developmental Biology 2023Quote: ... 150 μL of cell suspension was manually aspirated into the cell loading tip pre-filled with mineral oil (Sigma-Aldrich). Detailed fabrication protocol of cell delivery and droplet collection tips is provided in our recent article37.
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bioRxiv - Bioengineering 2023Quote: ... Protein-containing fractions were pooled and buffer-exchanged against HBS-E (10 mM HEPES at pH 7, 150 mM NaCl, 2 mM EDTA) by ultrafiltration using Amicon Ultra devices (Millipore).
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bioRxiv - Bioengineering 2023Quote: ... and fibrin support materials were prepared with FITC-labeled diffusants (either 1 µg/mL diazide-PEG-FITC, 10 µg/mL of 10, 20, 40, 60, 150, 250 kDa FITC-dextran, Sigma). 150 µL of the diffusant-laden support materials were loaded into a clear bottom ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... each whole brain was placed in a microtube containing 150 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma Aldrich) per brain ...