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Citations for Millipore Sigma :
601 - 650 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Lipids were profiled using an Acentis Express C18 150 x 2.1 m column (Millipore Sigma 53825-U) and the ID-X Tribrid mass spectrometer ...
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bioRxiv - Genomics 2024Quote: ... and 300µL of acid washed glass beads suspended in dH2O (150-212µm, Sigma Aldrich cat# G1145-10G), 350µL Acid Phenol:chloroform (5:1 ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed twice with PBS and lysed in RIPA buffer (150 mM Tris-HCl [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2024Quote: ... Analytes were separated using a SeQuant ZIC cHILIC 3μm 100Å 150 x 2.1 mm column (EMD Millipore) installed in an Ultimate 3000 RS UHPLC system (Dionex ...
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bioRxiv - Immunology 2024Quote: ... 150 μL of BrightGlo- TritonX solution (90% BrightGlo [Promega, PRE2620] + 1% Triton X [Sigma, T9284]+ 9% DPBS) was applied on top of each gel ...
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bioRxiv - Neuroscience 2024Quote: ... A Sequant ZIC-pHILIC column (2.1 mm i.d. × 150 mm, particle size of 5 µm, Millipore Sigma) was used for separation of metabolites ...
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bioRxiv - Bioengineering 2024Quote: ... were pre-coated with 150 μL 1 mg/mL Fibronectin + 4850 μL PBS (Sigma Aldrich #F1141-5MG) 2 hours prior to cell thawing ...
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bioRxiv - Neuroscience 2024Quote: ... Purified PAK6 was then eluted with in Buffer B supplemented with 150 µg/ml 3xFlag peptides (Sigma).
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bioRxiv - Synthetic Biology 2024Quote: ... 150 mM NaCl) using Amicon Ultra-15 centrifugal units (50 kDa/10 kDa respectively, Millipore Sigma; UFC9010). IgG/Fab protein was characterized for purity by SDS-PAGE with concentrations being determined by spectrophotometry (280 nm).
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bioRxiv - Neuroscience 2024Quote: ... A Sequant ZIC-pHILIC column (2.1 mm i.d. × 150 mm, particle size of 5 µm, Millipore Sigma) was used for separation of metabolites ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Discovery HS F5 column (2.1 mm i.d × 150 mm, 3 μm particle size, Sigma-Aldrich) coupled to a Q Exactive instrument (PFPP-LC/MS) ...
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bioRxiv - Cell Biology 2024Quote: ... lysed and resuspended in 150 μL of TRAPeze® 1X CHAPS Lysis Buffer (Chemicon®, Merck Millipore). Protein concentration of the samples was determined using the CB-X Protein assay (G-Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then blocked with 4i blocking solution (1% BSA + 150 mM maleimide (Sigma-Aldrich Cat# 129585) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... The SRB dye was dissolved using 150 μl of a 10 mM Tris base solution (Sigma, T1503), and absorbances were measured at 564 nm using a microplate reader (Synergy H1 Hybrid reader ...
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bioRxiv - Microbiology 2022Quote: ... lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1 mM EDTA; Millipore) supplemented with 1× protease inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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bioRxiv - Microbiology 2021Quote: ... plates were processed by transferring 150 ul of eluate from each well to a 0.2um filtration plate (Millipore, Multiscreen HTS GV 0.22um Filter Plate MSGVS22 ...
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bioRxiv - Genomics 2020Quote: ... After addition of 150 μl of Papain-Mix (30 U/ml Papain (Sigma Aldrich Chemie GmbH Munich, Germany), 0.24 mg/ml Cysteine (Sigma Aldrich Chemie GmbH Munich ...
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bioRxiv - Molecular Biology 2021Quote: ... The first immunoprecipitation was carried out at 150 mM KCl with 300 μL anti-FLAG resin (Sigma A2220) for 4 h at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.5, 150 mM NaCl, 1mM EDTA, 0.1% Nonidet P-40, 10% glycerol, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Plant Biology 2020Quote: ... tissue was ground in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 1% Plant Proteinase Inhibitor Cocktail [Sigma] ...
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bioRxiv - Developmental Biology 2020Quote: ... 50,000 cell aliquots were pelleted at 600xg in a swinging bucket rotor centrifuge and washed twice with 200 μL CUT&Tag wash buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine (Sigma); 1x Protease inhibitor cocktail (Roche)) ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 10 mM MgCl2, 150 mM KCl, 1% NP-40 [Sigma I8896-50ML], 0.5 mM DTT [Sigma, 646563] ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Bioengineering 2020Quote: ... We used the following primary and secondary antibodies and dilutions: mouse anti-α-tubulin 1:150 (Sigma-Aldrich), goat anti-collagen IV 1:50 (Southern Biotech) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed by scraping in a total volume of 80 µL of lysis buffer (containing 50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) and processed for immunoblotting as described in this manuscript under the PUM1 validation subchapter ...
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bioRxiv - Neuroscience 2021Quote: ... cells were scraped with 100 µL lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) supplemented with EDTA-FREE cOmplete Mini protease inhibitor cocktail (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Soils were amended with 150 µl of either 13C7-labeled p-hydroxybenzoic acid (99% atom 13C; Sigma-Aldrich) or unlabeled control (‘12C’ ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) or treated with 150 µg/mL of rifampicin (USBiological) and 45 µg/mL of cephalexin (Sigma) for at least 3 hours for replication run-out experiments ...
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bioRxiv - Microbiology 2021Quote: ... The reactions were stopped by adding to 5 μl of reaction 5 μl of Stop Solution (Tris HCl pH 7.5 100 mM, EDTA 12.5 mM, NaCl 150 mM, SDS 1%, Proteinase K (Sigma) 2 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 ng dsRNA/well was transfected into cells using 0.6 μL X-tremeGENE HP DNA Transfection Reagent (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was washed and resuspended in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween 20 (Sigma)) and pelleted again by centrifugation as before ...
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bioRxiv - Microbiology 2021Quote: Cellular proteins were extracted with a whole-cell extract buffer (50 mM Tris [pH 7.5], 150 mM NaCl, 0.5% Triton X-100, 10% glycerol, 1 mM EDTA, protease inhibitor cocktail [Sigma]). Cells lysates were resolved on 4–12% Bis-Tris NuPAGE gels (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... Polar metabolites were separated on a ZIC-pHILIC 150 × 2.1 mm (5-μm particle size) column (EMD Millipore) connected to a Thermo Vanquish ultrahigh-pressure liquid chromatography (UPLC ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was blocked with 3% bovine serum albumin in TBS-T buffer (50 mM Tris pH 7.5, 150 mM NaCl, 0.1% Tween-20) and incubated with anti-FLAG (Sigma, St ...
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bioRxiv - Bioengineering 2022Quote: Cells were washed once with PBS and lysed in cold RIPA lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1x protease inhibitor cocktail [Sigma]). Lysate was sonicated (Qsonica Q800 Sonicator ...
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bioRxiv - Biochemistry 2022Quote: ... Frozen cells suspended in ice-cold extraction buffer were disrupted with glass beads (diameter 150-212 μm, Sigma) via vortexing 30 s/5 cycles/each with 1-min break on ice ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... Cells were then lysed with ice-cold lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 0.4% NP-40 (Sigma), 5% glycerol ...
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bioRxiv - Immunology 2020Quote: ... Primary antibody in 250 μl of cold antibody buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 2 mM EDTA, 0.1% digitonin, and protease inhibitor cocktails from Sigma) was added to the tubes and rotated at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... 2×105 sorted cells were washed twice with 1 ml of cold wash buffer (20 mM HEPES-NaOH pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and protease inhibitor cocktails from Sigma) in 1.5ml tubes ...
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bioRxiv - Cancer Biology 2020Quote: ... Enzymatic removal of GCX components was achieved using hyaluronidase (H3631, Sigma Aldrich, used at 150 units mL-1), chondroitinase (C3667 ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was eluted using Buffer A with 150 mM NaCl and 200 μg/ml 3XFLAG peptide (Sigma). Peak fractions were pooled ...
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bioRxiv - Pathology 2021Quote: ... 150 µL ethanol (99.6%) and 25 µL silica-suspension (1g/ml silicon dioxide, Sigma-Aldrich S5631, pH 2) were added ...
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bioRxiv - Genetics 2021Quote: ... Drosophila S2R+ (CVCL_Z831, DGRC Cat # 150), S2 DGRC (CVCL_TZ72, DGRC Cat # 6) cells were grown in M3 media (Sigma) containing Bactopetone ...
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bioRxiv - Microbiology 2020Quote: ... Excess dansyl chloride was cleared by incubating the reaction with 100 μL 150 mg/mL proline (Sigma Aldrich). Dansylated polyamines were extracted with 50 μL toluene (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: Cells expressing GRAMD1C-EGFP or GRAMD1C(ΔGRAM)-EGFP were lysed with NP-40 lysis buffer (150 mM, 1.0% NP-40, 50 mM Tris-HCl pH 8.0) supplemented with PhosStop phosphatase inhibitor (Sigma) and complete protease inhibitor cocktail (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... were incubated at 4°C in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 1mM EDTA, 1 mM EGTA, 0.1% SDS, Sigma protease inhibitors 1:500 ...
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bioRxiv - Immunology 2020Quote: Whole Cell lysates were prepared with the RIPA buffer (50 mM Tris [pH 7.4], 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1mM EDTA with protease inhibitor cocktail [Sigma]), isolated from uninfected ...
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bioRxiv - Immunology 2020Quote: Cells were lysed in RIPA buffer (50 mM Tris.HCl, pH7.4; 150 mM NaCl; 1% NP-40 (Sigma-Aldrich), 0.5% Deoxycholate ...
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bioRxiv - Immunology 2021Quote: Lipids were dissolved by sonication in TBS (10 mM Tris pH 8.0, 150 mM NaCl) containing 0.05 % (v/v) tyloxapol (Sigma) and stored at −20°C prior to use ...