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Citations for Millipore Sigma :
701 - 750 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The patterned substrates were subsequently incubated with 150 μl of fibronectin (10 μg/ml, Sigma Aldrich, 341631, Austria) in PBS or vitronectin (3.3 µl/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Physiology 2024Quote: ... The samples were separated on a Discovery HS F5-3 column (2.1 mm I.D. x 150 mm L, 3μm particle, Sigma-Aldrich) using a step gradient of mobile phase A (0.1% formate ...
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bioRxiv - Microbiology 2024Quote: ... and Gray’s medium in the basal compartment was supplemented with 150 ng/mL retinoic acid (#R2625, Sigma-Aldrich). Cells were cultured at ALI for a minimum of 28 days prior to use ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The beads were washed twice with IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween 20 [Sigma]) by pelleting them on a magnetic rack and removing the supernatant ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The pellet was then washed and resuspended in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween 20 [Sigma]) and pelleted again as before ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were dissolved in 150 µl lysis buffer consisting of 1% (w/v) sodium deoxycholate (SDC, Sigma), 10 mM Dithiothreitol (DTT ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 150 mM NaCl and 5% (vol/vol) glycerol supplemented with 1mM Tris 2-carboxyethyl phosphine hydrochloride (TCEP, Sigma). Elution fractions were collected and evaluated by SDS-PAGE and relevant fractions with purified proteins were concentrated as appropriate for further studies.
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bioRxiv - Plant Biology 2020Quote: ... The sample was resuspended in ∼ 200 µl of ice cold extraction buffer (25 mM Tris pH 7.5, 150 mM NaCl, 1 % SDS, 10 mM DTT, protease inhibitor cocktail (P9599, Sigma)) ...
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bioRxiv - Cancer Biology 2021Quote: ... Pellets were thawed on ice and lysed in NP40 lysis buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 1% NP40 substitute, 1/250 mammalian protease inhibitor cocktail (Sigma)) ...
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bioRxiv - Cell Biology 2020Quote: HeLa cells were lysed on ice for 30 min in a HEPES buffer (150 mM NaCl, 50 mM HEPES pH 7.0, 1% IGEPAL CA-630, 1 mM nicotinamide (Sigma), 5 mM tris(2-carboxyethyl)phosphine hydrochloride (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were harvested with 500 µl of NET buffer (150 mM NaCl, 50 mM Tris pH7.5, 1 mM EDTA and protease inhibitor cocktail from Sigma). Cells were kept on ice for 15 minutes and lysed by 20 times up and down pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... 150 mM NaCl, 2 mM EGTA, 2 mM MgCl2, 0.5% NP-40, 1mM DTT, 1U/ml Benzonase from Novagen/EMD Millipore ...
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bioRxiv - Evolutionary Biology 2021Quote: ... which was pretreated with 0.01% poly-L-lysine (mol. wt. 150 K–300 K; Millipore Sigma, St. Louis, MO) at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested in TENT buffer (50 mM Tris-HCl pH 8.0, 2 mM EDTA pH 8.0, 150 mM NaCl, 1% Triton X-100; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl TUBEs beads/IP were washed twice with TBST (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween 20; Sigma-Aldrich) complemented with 1xPIC (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... Aliquots of stock solutions were complexed with fatty-acid–free BSA (10% solution in 150 mM NaCl; Sigma-Aldrich) by stirring for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by three washes in low-salt buffer (20 mM HEPES KOH, pH 7.4, 10 mM MgCl2, 150 mM KCl, 1% NP-40 [Sigma I8896-50ML] ...
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bioRxiv - Microbiology 2020Quote: ... BBMV were centrifuged and resuspended in binding buffer (20 mM Tris, 150 mM NaCl, 1 mM MnCl2, pH 7.4, 0,04% Blocking reagent from Sigma Aldrich). Competition binding experiments were conducted by incubating 1.4 μg of BBMV protein with 0.65 nM 125I-Vip3Aa in a final volume of 0.1 ml of binding buffer for 90 min at 25°C in the presence of increasing amounts of unlabeled Vip3Aa ...
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bioRxiv - Plant Biology 2020Quote: ... and ground in liquid N2 in 2 volumes of extraction buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 10% glycerol, 0.1% Nonidet P-40 and 1x protease inhibitor cocktail; Sigma-Aldrich) and centrifuged for 12,000 g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins were buffer exchanged into TBS (20 mM Tris-HCl, pH 7.0 and 150 mM NaCl) by Amicon Ultra-4 Centrifugal Filter Units (Millipore). The final concentrations of the proteins were determined by the BCA method.
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bioRxiv - Biophysics 2022Quote: ... Cell pellets resuspended in Lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1 mM PMSF, and EDTA-free SigmaFast protease tablets (Sigma)) were sonicated ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed with modified RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1mM EDTA, 1% NP-40, 0.25% deoxycholic acid; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was discarded and the membrane fractions were homogenized in 20 mL solubilization buffer (25 mM HEPES pH 7.5, 150 mM NaCl, 5% v/v glycerol) with cocktail inhibitors (Sigma) per initial liter of culture volume ...
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bioRxiv - Bioengineering 2021Quote: ... treatments were removed and either replaced with regular culture media or luciferin-containing media (150 μg/mL) (Sigma, L9504) for evaluation of luminescence by IVIS imaging.
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bioRxiv - Bioengineering 2020Quote: ... THP-1 cells were differentiated into macrophage-like cells with 150 nM phorbol 12-myristate 13-acetate (PMA) (Sigma) before the experiment.
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bioRxiv - Neuroscience 2021Quote: ... MultiBrain® cryosections were prepared with 30μ thickness for free-floating immunolabeling with anti-HA (3F10, 1:150, Sigma) and counter stained with hematoxylin/eosin ...
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bioRxiv - Cell Biology 2021Quote: ... Hydrophilic interaction chromatography (HILIC) on a SeQuant® ZIC®-pHILIC 150 × 2.1 mm column (Millipore Sigma, Cat# 1504600001) coupled with a guard 20 × 2.1 mm (Millipore Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were eluted twice for 30 minutes each at 58°C with 150 mM dithiothreitol (DTT; D0632, Sigma-Aldrich), 1% SDS ...
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bioRxiv - Plant Biology 2021Quote: ... then transferred to Petri dishes containing different solutions and kept in same cabinet: 150 μM methyl jasmonate (Sigma-Aldrich), 500 μM salicylic acid ...
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bioRxiv - Biochemistry 2020Quote: ... The plate was finally washed twice in 150 μL HBS-T and once in 150 μL HBS before adding 60 μL 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Sigma #T0440). After developing signal for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then pelleted and resuspended in 100–200 µl of lysis buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5 % IGEPAL CA-630 (Sigma-Aldrich), 1 tablet/50 ml cOmplete protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2020Quote: ... Transformants were selected in medium lacking uridine or uracil or in the presence of 150 μg/ml hygromycin B (Sangon) or 0.1 μg/ml pyrithiamine (Sigma). For the recycling usage of the selectable marker pyr4 ...
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bioRxiv - Plant Biology 2020Quote: ... The pellet was washed and resuspended in 1 ml of IP buffer (10% glycerol, 25 mM Tris pH 7.5, 1 mM EDTA, 150 mM NaCl, 0.1% Tween® 20 (Sigma)) and pelleted again by centrifugation as before ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted using 1.6 ml of extraction/washing buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Triton, 0.2% NP-40, 6 mM BME and 1x protease inhibitor cocktail [SIGMA, USA]) during 30 minutes at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... cells were washed in ice-cold PBS and lysed in lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% sodium deoxycholate (Millipore Sigma), 10 mM beta-glycerophosphate (Millipore Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... The column was rinsed 4 times with 200 μL TBS (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1x plant protease inhibitor cocktail (Sigma), 20 μM PuGNAc ...
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bioRxiv - Plant Biology 2021Quote: ... followed by total protein isolation with extraction buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% [v/v] NP-40 and 1:200 protease inhibitor cocktail from Sigma). Co-IP was carried out the same as for co-IP with protoplasts except that GFP-Trap agarose beads (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... The mobile phase buffer consisted of 150 mM AMAC in water and filtered using a 0.22 µm disposable membrane cartridge (Millipore) before use ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was blocked overnight at 4°C in TBS-T (20 mM Tris, 150 mM NaCl, pH 8.0, 0.1% Tween 20 (Sigma, P9416) plus 5% dry milk (Apex ...
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bioRxiv - Microbiology 2021Quote: ... and applying lysis buffer (20 mM Tris/HCl, pH 7.5, 150 mM NaCl, and 1% Nonidet P-40 (all from Sigma)) supplemented with EDTA-free protease inhibitor mixture cocktail (Roche) ...
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bioRxiv - Systems Biology 2020Quote: ... The equivalent of fifty OD600 of cells were resuspended in ice cold yeast lysis buffer (10 mM Tris-HCl pH 7.4, 150 mM NaCl, 0.5 mM EDTA (Millipore Sigma), 1% triton X-100 (Millipore Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... MSCs were resuspended in 150 μl saline and encapsulation occurred by mixing the MSCs with 1.7% sodium alginate (Sigma) in saline (107 cells/ml) ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were resolved on the Discovery HS F5-3 column (2.1 mmI.D. x 150 mmL, 3 μm particle, Sigma-Aldrich), using a step gradient with mobile phase A (0.1% formate ...
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bioRxiv - Cell Biology 2021Quote: ... for phosphorylated proteins immunoblots in Tris-buffered saline (TBS) (50 mM Tris, 150 mM NaCl, pH = 7.6) containing 0.1% Tween-20 (Sigma-Aldrich) (TBS-T ...
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bioRxiv - Cell Biology 2022Quote: ... Membrane was blocked for 30 min in TBS-T (20 mM Tris pH 8.0, 150 mM NaCl, 0.1% Tween 20 (Sigma, P9416) containing 5% milk (Genesee ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: ... were used at 14,000 x g and 4 °C to exchange purified protein samples to 150 mM ammonium acetate (99.99 %; Sigma-Aldrich), pH 7.2 ...
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bioRxiv - Plant Biology 2022Quote: ... The pellets were washed with cytosol extraction buffer for three times and resuspended in membrane extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0.5% NP40 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves samples were homogenized in liquid nitrogen and incubated with cytosol extraction buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 2 mM DTT, protease inhibitor cocktail (Sigma), 0% NP-40 (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were thereafter washed three times with 1 ml wash buffer (50 mM Tris/HCl, pH 7.5, 150 mM NaCl and 0.5 % Digitonin (Sigma-Aldrich) or 0.5% NP-40 (Sigma-Aldrich ...