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Citations for Millipore Sigma :
651 - 700 of 1367 citations for pMHC1 150 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... in the positive mode and SeQuant ZIC-pHILIC column (150 mm×2.1 mm, 5 μm, Merck Millipore, 150460) in the negative mode ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... The stimuli were applied by placing 150 µL of water or odorant on filter paper (Sigma-Aldrich, #WHA10347509) in 35 mm petri dishes ...
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bioRxiv - Neuroscience 2023Quote: ... ZooPharm) and 150 uL local anesthetic under the scalp (0.25 bupivacaine hydrochloride saline solution, Sigma-Aldrich B5274-1G). Eyes were covered with Vaseline ...
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bioRxiv - Plant Biology 2023Quote: ... in wash buffer (20 mM Tris–HCl at pH 7.5, 150 mM NaCl, 5mM MgCl2, 1 mM DTT, 0.5% Triton x-100, proteinase inhibitor cocktail; Sigma). RNA was extracted from the bead-bound AGO1 with TRIzol reagent (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... Tissues were collected and ground in liquid nitrogen before resuspension in 5 ml of extraction buffer (20 mM Tris–HCl at pH 7.5, 150 mM NaCl, 5mM MgCl2, 1 mM DTT, 0.5% NP40, proteinase inhibitor cocktail; Sigma). The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche ...
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bioRxiv - Genetics 2023Quote: ... 150 x 4.6 mm with a 20 x 2.1 mm ZIC-pHILIC guard column (both Merck Millipore, Australia) (25 °C) ...
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bioRxiv - Immunology 2023Quote: ... baits were incubated with 75 μg nuclear extract in PBB buffer (150 mM NaCl, 50 mM Tris–HCl pH 7.5, 5 mM MgCl2, 0.5% Igepal CA-630 (Sigma) and 1 mM DTT ...
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bioRxiv - Plant Biology 2022Quote: ... water was replaced by 150 μL of assay solution containing 2 μg/mL horseradish peroxidase (Sigma-Aldrich, Germany) and 100 μM luminol (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Molecular Biology 2024Quote: ... and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer, pH 7.5, 150 mM KCl) that was supplemented with 0.05 % Tween-20 (Sigma) for use in SPR analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... and buffer exchanged at the same time into binding buffer (20 mM phosphate buffer, pH 7.5, 150 mM KCl) that was supplemented with 0.05 % Tween-20 (Sigma) for use in SPR analysis.
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bioRxiv - Neuroscience 2023Quote: ... in the positive mode and SeQuant ZIC-pHILIC column (150 mm × 2.1 mm, 5 μm, Merck Millipore, 150460) in the negative mode ...
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bioRxiv - Molecular Biology 2024Quote: ... (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 1 mM EDTA, 1 mM EGTA, 0.1% SDS, Sigma protease inhibitors P8340 1:500 ...
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bioRxiv - Molecular Biology 2024Quote: ... For liquid chromatography a SeQuant ZIC-pHILIC 5 μm Polymeric column 150 * 2.1 mm (Merck Millipore, Darmstadt, Germany) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were resuspended in 35 µL of TBS buffer (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) supplemented with 0.5 µg/µL FLAG peptide (Sigma) and incubated at 4 °C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... male and female mice were treated with tamoxifen (i.p., 150 mg/kg, Sigma Aldrich; solubilized in safflower oil) at two time points 48 hr apart ...
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bioRxiv - Microbiology 2024Quote: ... and lysed in 1 mL cold Wash Buffer (50 mM Tris HCL pH 7.5, 150 mM NaCl, 1 mM EDTA) plus 0.5% IGEPAL (Sigma), Complete protease (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 150 mM NaCl, 0.1 % SDS, 0.5 % Deoxycholic acid, 1 % Igepal and 1% protease inhibitor cocktail; Sigma Mammalian Cell lysis kit ...
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bioRxiv - Immunology 2023Quote: ... Cells were seeded at 8-8.5×106 cells in 75 cm² or 16.5×106 cells in 150 cm² culture flasks (TPP) coated with 0.25% fibronectin from human plasma (Sigma) and 1% extracellular matrix from Engelbreth-Holm-Swarm murine sarcoma (Sigma ...
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bioRxiv - Genetics 2023Quote: ... Nuclear extracts were incubated overnight at 4°C with 150 µl anti-FLAG M2 magnetic beads (M8823, Sigma) that were pre-washed with 1 mL TEGN buffer (20 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Beads-bound cells were resuspended in 100 μl of antibody buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.06% Digitonin (Sigma-Aldrich), 2 mM EDTA ...
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bioRxiv - Microbiology 2022Quote: ... 150 μM (2-(2-nitro-1H-imidazol-1-yl)-N-(2,2,3,3,3-pentafluoropropyl) acetamide (EF5 compound; Sigma-Aldrich, EF5014), 0.5 μM MitoSOX™ fluorescent probe (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed with PBS (150 µl/well) twice and incubated with DAPI nuclear stain (Sigma Aldrich, #D9542) at RT for 5 mins (50 µl/well) ...
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bioRxiv - Immunology 2022Quote: ... 10-week-old male mice were administered two doses of streptozotocin (STZ) (Sigma, 150 mg/kg body weight) in 50mM sodium citrate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... 1.5 mg protein lysate was incubated with 150 μl bead bed of pre-equilibrated hemin-agarose (Sigma, #H6390) for SILAC “heavy” group and the same bead bed volume of pre-equilibrated Sepharose (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were again washed twice with PBS and then stained with 150 nM Nile Red (Sigma-Aldrich, 72485) in PBS for 15 minutes at RT ...
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bioRxiv - Plant Biology 2022Quote: ... Transparent leaf segments were washed three times in 150 mM K2HPO4 (pH 9.6) and stained with 0.02 % aniline blue (cat. no: B8563-500ML; Sigma) in 150 mM K2HPO4 (pH 9.6) ...
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bioRxiv - Molecular Biology 2023Quote: ... MSK-QRESS-KIT] and 1μL was injected into a ZIC-pHILIC 150 × 2.1mm (5μm particle size) column (EMD Millipore) operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... in wash buffer (20 mM Tris–HCl at pH 7.5, 150 mM NaCl, 5mM MgCl2, 1 mM DTT, 0.5% Triton x-100, proteinase inhibitor cocktail; Sigma). RNA was extracted from the bead-bound EVs with TRIzol reagent (Invitrogen) ...
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bioRxiv - Plant Biology 2023Quote: ... Tissues were collected and ground in liquid nitrogen before resuspension in 5 ml of extraction buffer (20 mM Tris–HCl at pH 7.5, 150 mM NaCl, 5mM MgCl2, 1 mM DTT, 0.5% NP40, proteinase inhibitor cocktail; Sigma). The extract was then precleared by incubation with 50 ul of Protein A-agarose beads (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... After washing twice with 1 mL of wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine (Sigma) and protease inhibitors) ...
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bioRxiv - Biochemistry 2023Quote: ... Washed platelets were obtained by addition of 150 μL of citrate-dextrose solution (C3821, Sigma-Aldrich, Dorset, UK) and 5 μL of prostaglandin E1 (P5515 ...
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bioRxiv - Neuroscience 2023Quote: A localized chronic inflammatory pain state was established by injecting 150 μL of 50% complete Freund’s adjuvant (Sigma) in 0.9% saline subcutaneously into the intraplantar surface of the left hindpaw ...
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bioRxiv - Cancer Biology 2022Quote: ... Coverslips were first incubated with anti-pSR (1:150) and anti-CLK2 (1:500, Sigma Aldrich, HPA055366-100UL) antibodies for 1 hr ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei from 5x105 cells were isolated with wash buffer (20 mM HEPES-KOH, pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1x protease inhibitor cocktails from Sigma), captured with Concanavalin A conjugated paramagnetic beads (Bangs Laboratory Inc. ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking in TBS-T (50 mM Tris-HCl, 150 mM NaCl, pH 7.5, 0.1 % Tween-20 (Sigma)) supplemented with 5 % non-fat dry milk ...
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bioRxiv - Cancer Biology 2023Quote: ... A 5 µL aliquot of each sample was injected onto a ZIC-pHILIC column (150 mm × 2.1 mm i.d. 5 μm, Millipore) fitted with a guard column (20 mm × 2.1 mm i.d ...
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bioRxiv - Cancer Biology 2023Quote: ... A hydrophilic interaction chromatography method with a ZIC-pHILIC column (150 x 2.1 mm, 5 µm; EMD Millipore) was used for compound separation at 35 °C with a flow rate of 0.3 mL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads-bound cells were resuspended in 100 µl of antibody buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 0.06% Digitonin (Sigma-Aldrich), 2 mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... 150 mM NaCl, 0.5 mM Spermidine, 0.02% digitonin, 2 mM EDTA and 1x protease inhibitor cocktails from Sigma) overnight ...
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bioRxiv - Microbiology 2024Quote: ... beads were resuspended in 35 µL TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.5) with 0.5 µg/µL FLAG peptide (Sigma) and incubated at room temperature for 15 min at 900 rpm ...
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bioRxiv - Immunology 2024Quote: ... We chromatographically separated samples by injecting a sample volume of 1µL into a SeQuant ZIC-pHILIC Polymeric column (150 x 2.1mm 5µM, EMD Millipore). We set flow rates to 150µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or analysed through cation exchange chromatography using a PRP-X200 column (150 × 4.6 mm, 10 μm particle size; Hamilton, US) and 2.5mM pyridine (99.8%, Sigma-Aldrich) as mobile phase and the pH adjusted to 2.5-2.7 with the help of formic acid ...
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bioRxiv - Developmental Biology 2024Quote: ... Additional treatment with sonication (Branson SFX 150 Digitaler Sonifier, Emerson, MO, USA) and/or chymotrypsin/chitinase (Sigma-Aldrich) was included in some experiments ...
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bioRxiv - Microbiology 2024Quote: ... and Gray’s medium in the basal compartment was supplemented with 150 ng/mL retinoic acid (#R2625, Sigma-Aldrich). Cells were cultured at ALI for a minimum of 28 days prior to use ...
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bioRxiv - Microbiology 2024Quote: ... The patterned substrates were subsequently incubated with 150 μl of fibronectin (10 μg/ml, Sigma Aldrich, 341631, Austria) in PBS or vitronectin (3.3 µl/ml ...
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bioRxiv - Biochemistry 2024Quote: ... protein lysates from CHO cells were prepared by lysing cells in RIPA buffer (10 mmol/L Tris-HCl [pH 7.4], 150 mmol/L NaCl, 0.5% sodium deoxycholate, 0.1% SDS, and 1X cOmplete protease inhibitor cocktail [Sigma Aldrich]) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 150 mM NaCl) containing a protease and phosphatase inhibitor cocktail (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail, Millipore Sigma, #COEDTAF-RO ...
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bioRxiv - Molecular Biology 2024Quote: ... All immunoblotting was performed in 5% milk powder or 5% BSA in Tris-Buffered Saline (TBS; 20 mM Tris, pH 7.4, 150 mM NaCl)+ 0.1% Tween-20 (Sigma). Washes were done in TBS + 0.1% Tween-20 (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... The resuspension buffer was removed using a magnetic stand and beads were resuspended in 100 μl antibody buffer (20 mM HEPES, 150 mM NaCl, 0.5 mM spermidine, 0.01% digitonin (cat. 300410, Millipore), 2 mM EDTA (cat ...