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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and a mouse monoclonal antiserum against dopamine beta-hydroxylase (DbH; 1:5000; Millipore, MAB308; AB_2245740), followed by a cocktail of Cy3-conjugated donkey anti-rabbit Cy3 (red ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated overnight with the following antibodies: Beta-actin (1:1000; EMD Millipore, MO), Proinsulin (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were resuspended in 1% beta-Mercaptoethanol (#M3148-100ml, Sigma, part of Merck, Darmstadt, Germany)-supplemented RLT lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-beta tubulin III (TUJ1) 1:800 for IF (Sigma-Aldrich Cat# T8660, RRID:AB_477590), rabbit anti-DAGLB 1:1000 for WB and 1:800 for IF (Abcam Cat# ab191159) ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated over-night with primary antibodies: anti-beta-tubulin (Sigma, T6074, 1:1000 dilution) anti-EB3 (ATLAS anti-MAPRE3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blotted with antibodies against beta-actin for equal loading control (1:5,000, Sigma) [38].
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bioRxiv - Biochemistry 2024Quote: ... The mouse monoclonal antibody anti-beta Actin was obtained from Sigma-Aldrich (1:2000, A5316). The rabbit polyclonal anti-calnexin antibody was purchased from Enzo (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used: α-beta-III-tubulin (1:200, Sigma-Aldrich T2200), α-MAP2 (1:1.000 ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2022Quote: Fixation was performed following 24 hrs of recombinant protein treatment using 4 % PFA / 4% Sucrose (Sigma) in 1x PBS at RT for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant protein was concentrated by centrifugation in Amicon® Ultra-15-30K (Millipore Sigma, Burlington, MA), purified on protein A/G columns (NAb™ Spin Kit ...
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bioRxiv - Biochemistry 2024Quote: Recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼100 μM ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions containing recombinant protein were pooled and concentrated on a 30K Amicon Ultra Centrifugal filter (Millipore). Glycerol at 10% final was added to the sample and protein concentration was estimated using Bradford detergent compatible assay (Pierce) ...
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bioRxiv - Immunology 2024Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Immunology 2021Quote: Zebrafish larvae were anaesthetised in 250 μg/mL MS-222 (Tricaine, Sigma) and euthanised either by fixation in 4% paraformaldehyde ...
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bioRxiv - Genomics 2020Quote: ... and the zebrafish were fixed in formaldehyde 4% (#F1635-500ML, Sigma-Aldrich) for 1h at RT (8-12dpf larvae ...
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bioRxiv - Developmental Biology 2021Quote: Zebrafish embryos were embedded in 0.6% agarose low gelling temperature (A0701, Sigma) with 0.16 mg ml−1 Tricaine in E3 medium ...
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bioRxiv - Neuroscience 2021Quote: ... Zebrafish larvae 7dpf were briefly anesthetized using 0.3mM Pancuronium bromide (Sigma-Aldrich) for 2-3 min until the touch response stopped ...
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bioRxiv - Neuroscience 2020Quote: Adult zebrafish (6 months old) were anaesthetized in 0.016% tricaine (Sigma, MS222), and a spinal cord crush injury was performed according to a previously described method (Fang et al. ...
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bioRxiv - Immunology 2022Quote: ... zebrafish were anaesthetised in 0.168 mg/ml Tricaine (MS-222, Sigma-Aldrich) in E3 media and transferred onto 1% agarose in E3+methylene blue plates ...
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bioRxiv - Cancer Biology 2022Quote: ... live zebrafish embryos were embedded in 0.7% low melting agarose (Sigma-Aldrich) dissolved in 1x Ringer’s solution ...
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bioRxiv - Neuroscience 2021Quote: ... adult zebrafish were incubated in system water containing 10 mM metronidazole (Sigma) for 24 hrs in a 28 °C incubator ...
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bioRxiv - Neuroscience 2021Quote: ... Zebrafish larvae were exposed to 0.2-0.35 µM disulfiram (86720, Sigma-Aldrich) from 2 dpf to 6 dpf ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish larvae were soaked into 3 nM Chicago Sky Blue 6B (Sigma), 5 μM MPEP (Cambridge Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... Zebrafish embryos were mounted in 1.5% low-melting agarose (Sigma-Aldrich, A9045) into 35 mm glass-bottom confocal dish or glass capillary before imaging ...
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bioRxiv - Cancer Biology 2021Quote: ... zebrafish larvae were anaesthetized with benzocaine (200 mg/L; Sigma, Cat#PHR1158) and mounted in 1% agarose ...
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bioRxiv - Cell Biology 2024Quote: ... zebrafish embryos were anesthetized with 200 mg/l Tricaine (Sigma Aldrich, E10521) during imaging58 ...
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bioRxiv - Cell Biology 2024Quote: ... Zebrafish were euthanized with 1g/L of MS-222 (Sigma, MO, USA), followed by fixation in 10% neutral buffered formalin for 72 hr and decalcified in 0.5M EDTA for 48 hr at room temperature ...
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bioRxiv - Genetics 2022Quote: ... We euthanized the zebrafish juveniles with 250 mg/L tricaine (Sigma-Aldrich) in E3 medium ...
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bioRxiv - Immunology 2023Quote: ... zebrafish were anaesthetised in 0.168 mg/ml Tricaine (MS-222, Sigma-Aldrich) in E3 media and were transferred onto 1% agarose in E3+methylene blue plates ...
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bioRxiv - Developmental Biology 2023Quote: The zebrafish melanocytes were ablated with Neocuproine (NCP; Sigma-Aldrich, MO, USA), a copper chelating agent that specifically kills mature melanocytes without giving harm to mitf+ melanocyte stem/progenitor cells (O’Reilly-Pol and Johnson 2008 ...
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bioRxiv - Immunology 2023Quote: ... Zebrafish larvae were anaesthetised in 0.168mg/ml Tricaine (MS-222, Sigma-Aldrich) in E3 media ...
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bioRxiv - Cancer Biology 2024Quote: ... zebrafish larvae were anesthetized in 40 μg/mL tricaine (Sigma, #E10521-50). Anesthetized zebrafish larvae were placed randomly on the agarose gel plate ...
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bioRxiv - Cancer Biology 2024Quote: Adult zebrafish were fixed in 10% neutral buffered formalin (NBF) (Sigma, HT501128) for a minimum of ten days ...
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bioRxiv - Cell Biology 2020Quote: ... wildtype AB zebrafish embryos microinjected with pig tubulin-atto565 were mechanically dechorionated and mounted in 1% low melting-point agarose (Sigma) in E3 medium supplemented with 20% w/v Iodixanol (Sigma ...
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bioRxiv - Immunology 2020Quote: Wild-type zebrafish embryos were dechorionated at 24 h post fertilization (hpf) using digestion with 1 mg/ml pronase (Sigma) in E3 medium (83 μM NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Protein from gels were transferred onto PVDF membranes and probed with NSF (SYSY, mouse monoclonal) and beta actin (Sigma, mouse monoclonal) antibodies overnight with shaking ...
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bioRxiv - Cell Biology 2021Quote: ... The beta subunit of complex V and proteins bound to it were immunoprecipitated using an antibody to the beta subunit of ATP synthase (Millipore, MAB3494) in accordance with the Pierce™ Co-Immunoprecipitation Kit (Cat.No:26149) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 1 hr at 37°C with mouse anti-beta-Tubulin (#05-661; Millipore; 1:1500) primary antibody diluted in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted by cleavage of the GST tag by 15 units/ml TEV protease (Sigma T4455-1KU) in PBS buffer overnight at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... and mouse anti-His (Novagen).
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was determined using BCA™ protein assay (Pierce, Rockford, IL, USA) using BSA (Sigma- Aldrich, St. Louis, MO, USA) as a standard ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Microbiology 2020Quote: Antibodies against LeishIF4E2(rabbit polyclonal, 1:2,000) and against the SBP tag (Millipore, monoclonal, 1:10,000), were used to detect the endogenous and tagged LeishIF4E2 proteins ...
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bioRxiv - Genomics 2023Quote: ... Flag-Tag (F1804, 1:2,000 for Western blot, 1:200 for immunofluorescence) was from Sigma-Aldrich. Anti-mouse IgG–HRP (7076S ...