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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Immunology 2024Quote: ... 1:1000 mouse anti-6 × His (Sigma, St Louis, MO, USA) or 1:1000 mouse anti-GST (TransGen Biotech ...
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bioRxiv - Cancer Biology 2020Quote: ... and zebrafish tumors were mechanically homogenized in RIPA lysis buffer containing 1:100 protease inhibitors (P8340, Sigma-Aldrich) and 20 μM N-ethylmaleimide (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... Zebrafish aged 8-9 months (adult) or 1 month (juvenile) were culled in high dose tricaine (Sigma Aldrich), immersed for 5 min in 1% Virkon (3S Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... zebrafish embryos were treated with 180 µM of 1-phenyl 2-thiourea (PTU) (Sigma-Aldrich, 103-65-5), to prevent pigmentation [25] ...
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bioRxiv - Biophysics 2024Quote: Staged zebrafish larvae at 56 or 72 hpf were anesthetized and then euthanized with 1% MS-222 (Sigma). Then ...
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bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Molecular Biology 2024Quote: ... The expressed protein was confirmed by Western blot analysis using anti-FLAG-tag antibodies from Sigma. The centromeric repeat pAL1 was amplified from Arabidopsis Col-0 genomic DNA using IRD700 labeled and unlabeled oligos ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2024Quote: ... 1% P/S and 1% GlutaMax and 2 μM Cytosine beta-D-arabinofuranoside (AraC; Sigma, #C1768-100MG) to inhibit glial cell growth ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-histidine tag 1:200× (Sigma-Aldrich, St. Louis, MO) and HRP-conjugated goat anti-mouse IgG 1:1,000× (Seracare Life Sci. ...
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bioRxiv - Microbiology 2023Quote: ... rabbit polyclonal anti-FLAG-tag (#F7425, Sigma-Aldrich, dilution 1:1,000), rabbit polyclonal anti-EGFP (#2555 ...
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bioRxiv - Cell Biology 2024Quote: ... His-3C was depleted on the His-Select Affinity matrix (Sigma-Aldrich) prior to Size Exclusion Chromatography on a HiLoad 16/60 Superdex200 column (Cytiva ...
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bioRxiv - Cell Biology 2020Quote: ... and beta-actin (catalog #A5441) (Sigma-Aldrich, St. Louis, MO, USA; dilution 1:10,000). Signals were revealed using peroxidase-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... both at 1:1000 and anti-beta actin mouse monoclonal antibody (Sigma-Aldrich; RRID:AB_476697) at 1:10,000 dilution.
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bioRxiv - Cell Biology 2021Quote: ... and beta-actin (1:10,000; A5441) were purchased from Sigma Aldrich (Sant Luis, USA). Secondary antibodies coupled to horseradish peroxidase used for immunoblotting were obtained from Sigma (A4914 Goat anti-Rabbit IgG).
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bioRxiv - Cell Biology 2021Quote: ... anti-alpha 2 integrin (RRID:AB_11212896) and anti-beta 1 integrin (RRID:AB_91150) all from Millipore (MilliporeSigma ...
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bioRxiv - Neuroscience 2024Quote: ... Anti-Beta-Actin conjugated with HRP 1:25,000 (Sigma-Aldrich, A3854 (clone AC-15), anti-Sox2 (Millipore Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-beta Actin (Clone C4) (1:10.000, MAB1501, Millipore, Burlington, Massachusetts, USA). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... Transcription inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) powder (Millipore Sigma, D1916-50MG) was dissolved in DMSO for a final stock concentration of 100mM ...
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bioRxiv - Biophysics 2024Quote: ... 1.2 M betaine) and solubilised with 1% (w/v) n-Decyl-Beta-Maltoside (Sigma) at 4°C in the dark with constant stirring for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... suis antibody (1:100) and a mouse anti-tubulin beta III antibody (Merck Millipore, Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... transgenic zebrafish were anaesthetised with 0.01% Tricaine (Sigma, catalogue number E10521) and embedded in 1% low-melting agarose (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... zebrafish were dechorionated using 2 mg/ml pronase (Millipore Sigma, #10165921001) in PTU fish water for injection into the caudal vein of the zebrafish to deliver cells directly into the circulation.
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bioRxiv - Genetics 2024Quote: ... zebrafish larvae were suspended in 3% Methyl Cellulose (Sigma Aldrich #M0387) solution.
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bioRxiv - Biochemistry 2020Quote: ... HIS (Sigma-Aldrich H3667), purified full-length Vn (1 μM in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Anti-His (Millipore, USA), anti-GST (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... or anti-His (Novagen) antibodies to detect Ub conjugates.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies.
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bioRxiv - Plant Biology 2020Quote: ... and anti-His (Sigma) antibodies at a 1:2000 dilution ...
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bioRxiv - Plant Biology 2022Quote: ... anti-His (Sigma H1029), anti-GFP (Agrisera ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Cell Biology 2023Quote: ... and the His-labelled prey proteins were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen-Millipore, 70954) and incubated in liquid cultures ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...
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bioRxiv - Microbiology 2022Quote: ... 500ng of the spike protein was treated with 100ng recombinant human cathepsin-L (Sigma-Aldrich) in sodium acetate buffer (pH 5.5 ...
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bioRxiv - Plant Biology 2020Quote: ... The expression of recombinant protein was induced by 0.2% (w/v) L-Arabinose (Sigma Aldrich). The expressed protein was purified as His-tagged fusion protein using Ni-NTA agarose beads (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... The detection of recombinant proteins was analyzed by immunoblot using an anti-myc antibody (Sigma) for SyNOS and a specific OtNOS antibody generated by GenScript Company.
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bioRxiv - Neuroscience 2021Quote: ... The recombinant proteins were purified with anti-FLAG agarose affinity gel (A2220; Sigma-Aldrich Japan), eluted with FLAG peptide (A3290 ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins (PRMT5:MEP50 complex (SRP0145-25470) and EZH2 (SRP0379) were purchased from Sigma-Aldrich USA ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified recombinant proteins were analyzed by SDS-PAGE and concentrated using stirred ultrafiltration cells (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant proteins EZH2 (SRP0379) and PRMT5:MEP50 complex (SRP0145-25470 were purchased from Sigma-Aldrich United states ...
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bioRxiv - Biochemistry 2024Quote: All recombinant proteins were expressed as GST fusions using BL21(DE3) Escherichia coli (Sigma-Aldrich) and purified using glutathione-agarose beads ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was cleaved from the GST tag by incubating the beads with human thrombin (Sigma-Aldrich) in the presence of 50 µM CaCl2 overnight at 4 °C with rocking ...
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bioRxiv - Molecular Biology 2024Quote: ... The FLAG-tag containing proteins were visualised using an Anti-FLAG M2-mcAb-HRP antibody (Sigma Aldrich).