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Citations for Millipore Sigma :
1001 - 1050 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Samples were loaded to have roughly similar band intensity for the band of interest to enable better estimation of relative protein size (recombinant pro-conA: 0.75 μg, conA (Sigma): 1.5 μg ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was used as the host for expressing recombinant proteins encoded in the pET30a vector (Novagen, USA). Appropriate antibiotics were added when needed at the following concentrations ...
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bioRxiv - Immunology 2020Quote: DHFR activity of the recombinant β-lactamase-DHFR fusion protein was evaluated using the DHFR Assay Kit (Sigma-Aldrich), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... imidazole 5 mM and recombinant RAD51-proteins were purified on a NiNTA resin (#P6611, Sigma, St Louis, MO, USA) using the washing buffer ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Cell Biology 2024Quote: Two Guinea pigs were each immunized with 0.2 mg of purified recombinant protein mixed with equal volume of Freund’s Complete Adjuvant (Sigma F5581), followed by two boosts of 0.1 mg antigen mixed with equal volume of Freund’s Incomplete Adjuvant (Sigma F5506 ...
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bioRxiv - Immunology 2020Quote: ... 1 × AA and 50 U/ml IL-2 (Sigma-Aldrich; MO, USA), were treated in triplicate respectively with D+T NP or D+T solution ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-TMIGD1 constructs in pFlag-CMV-1 (N-terminal Flag tag, Sigma-Aldrich, Munich, Germany): hTMIGD1 without signal peptide (AA 30-262 ...
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bioRxiv - Bioengineering 2021Quote: ... The coding sequences for the His6-Tag were amplified with the pCOLADUETTM-1 (Merck/Millipore) plasmid backbone ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary anti-Flag tag antibody was obtained from Sigma-Aldrich (F7425-.2MG, WB 1:2000). Primary antibodies against viral proteins were obtained from ...
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bioRxiv - Biochemistry 2023Quote: ... full-length REC114 and mutants were cloned as Strep-tag fusions into pRSFDuet-1 (Novagen). MEI41-43 and its mutants were cloned as His-MBP fusions in pETM41 ...
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bioRxiv - Genetics 2024Quote: ... Western blots were reacted with monoclonal anti-S-tag (1:5,000; SAB2702227, Sigma-Aldrich®) as primary antibody and with polyclonal anti-mouse IgG peroxidase (1:5,000 ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were mixed with protein A/G agarose beads (Pierce, Waltham, MA, USA) conjugated to antibodies against FLAG-tag (Sigma-Aldrich) or Exo70 (Santa Cruz ...
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bioRxiv - Plant Biology 2023Quote: The sequences encoding mature proteins were cloned into vector pET-15b with an N-terminal His6 tag sequence (Novagen, Darmstadt, Germany) (primer sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies include: anti-V5 Tag (Invitrogen, ref R96025, 1:500, anti-HA (Sigma-Aldrich, ref H6908, 1:500), anti-Akt (pan ...
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bioRxiv - Cell Biology 2023Quote: ... anti-HA tag (CST, 2367, 1:1000 for WB) and anti-β-actin (Sigma, 2228, 1:2000 for WB) antibodies followed by incubation with HRP-conjugated secondary antibodies (CST ...
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bioRxiv - Developmental Biology 2021Quote: ... 4% PFA fixed zebrafish embryos were embedded in 6% low-melting agarose (LMA, Sigma-Aldrich) dissolved in 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: Zebrafish were sacrificed by lethal dose of 1g/L of MS-222 (Sigma, MO, USA) and each tissue (gonads ...
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bioRxiv - Cell Biology 2022Quote: Zebrafish were sacrificed by lethal dose of 1g/L of MS-222 (Sigma, MO, USA), fixed for 72 hr in 10% neutral buffered formalin and decalcified in 0.5M EDTA for 48 hr at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Sequences were PCR amplified from zebrafish genomic DNA using the primers in SupplementaryTable16 (Sigma-Aldrich), with the proof-reading iMax ™ II DNA polymerase (INtRON Biotechnology ...
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bioRxiv - Neuroscience 2020Quote: ... Zebrafish older than 5 dpf were deeply anesthetized using 0.2% tricaine methanesulfonate (MS222; Sigma-Aldrich) diluted in fish water ...
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bioRxiv - Genetics 2020Quote: ... Morpholino injected zebrafish embryos were placed in fish water containing 1x PTU (Sigma; Cat# P7629) at twenty-four hours post fertilization ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish larvae were anaesthetised in PBS containing 0.02 % aminobenzoic-acid-ethyl methyl-ester (MS222, Sigma), as described (Tsarouchas et al. ...
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bioRxiv - Immunology 2021Quote: ... The zebrafish embryos were maintained in egg water containing 0.2 mM N-phenylthiourea (PTU; Sigma) to prevent pigment formation at 28 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and zebrafish larvae were treated with chloroquine diphosphate (3 mM, Sigma-Aldrich [catalogue number C6628]), two commonly utilised inhibitors of autophagy [32] ...
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bioRxiv - Cancer Biology 2021Quote: ... TO(krasG12V) zebrafish larvae (21) were treated with 20 μg/ml doxycycline (Sigma, Cat#D9891) at 2 dpf in egg water with 0.003% 1-Phenyl-2-thiourea (PTU ...
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bioRxiv - Genetics 2020Quote: ... zebrafish larvae at 6 or 9 dpf were exposed to calcein (2g/L) (Sigma-Aldrich) in water for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... zebrafish larvae were either untreated or treated with 2,4,6- Trinitrobenzenesulfonic acid (TNBS; Sigma Aldrich P2297) from day 3 post-fertilization until 120 hpf ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... zebrafish were washed with egg water and then treated with 7.5 mM metronidazole (MTZ) (Sigma) prepared in egg water or egg water alone as described previously [22] ...
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bioRxiv - Neuroscience 2022Quote: ... 7-8dpf zebrafish larvae were anesthetized by briefly immersing them in 0.017% MS222 (Sigma-Aldrich) and embedded dorsal side up in a drop of 2% low gelling temperature agarose (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: The Zebrafish larvae were mounted on a microscope slide 2% low-melting-point agarose (Sigma) and covered with coverslip ...
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bioRxiv - Cell Biology 2024Quote: 9-month-old fish zebrafish were sacrificed in 1g/L of MS-222 (Sigma Aldrich), and the skin was collected in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Hepatocyte injury was performed by incubating the zebrafish larvae with 10 mM acetaminophen (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Developmental Biology 2024Quote: ... the zebrafish were anesthetized with 0.04% Tricaine (ethyl 3-aminobenzoate methanesulfonate salt, #A5040, Sigma, USA) in system water and given i.p ...
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bioRxiv - Neuroscience 2024Quote: ... Zebrafish larvae at 6dpf were anesthetized using 0.3mM Pancuronium bromide (Sigma-Aldrich, Oakville, ON, Canada) for 2-3 min until the touch response stopped ...
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bioRxiv - Neuroscience 2024Quote: ... zebrafish larvae were embedded in 1.5% w/v low gelling temperature agarose (Sigma-Aldrich, A9414), placed on a microscope slide oriented dorsal side up and submerged in fish water ...
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2021Quote: ... and anti-His HRP conjugated (Sigma-Aldrich) antibodies ...
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bioRxiv - Microbiology 2021Quote: ... HIS-Select® Nickel Affinity resin (Sigma) and Streptactin column material (IBA GmbH ...
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bioRxiv - Plant Biology 2020Quote: ... and purified on His•Bind resin (Novagen) in the presence of 8 M urea according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... then detected with anti-His antibody (Sigma). S protein expression was detected with anti-SARS-CoV-2 S protein S2 subunit mouse mAb unconjugated (GeneTex) ...
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bioRxiv - Immunology 2020Quote: ... with added 10% HI FBS (Sigma-Aldrich) and 1% Pen/Strep (Lonza) ...
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Biochemistry 2022Quote: ... anti-His (Millipore: 05-949, clone HIS.H8) or Goat anti-Mouse IgG (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... L-histidine (L-his, Sigma-Aldrich #H8125), L-ornithine (L-orn ...