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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... Milliplex magnetic bead panel assays (CCL2, CXCL10, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-6, and IL-10; Millipore Sigma, Burlington, MA) were used as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Immunology 2024Quote: ... expanded with recombinant human IL-2 (1,000 U/mL; Proleukin) in the presence of rapamycin (50 nmol/L; Sigma-Aldrich). After 2 days ...
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bioRxiv - Immunology 2020Quote: ... The bacterial culture was then induced to produce the recombinant protein by the addition of 0.5 M isopropyl β-d-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich) and was allowed to grow for a further 3 h ...
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bioRxiv - Genetics 2020Quote: Adult zebrafish were euthanized using tricaine (MS-222, Sigma-Aldrich). Tissue was fixed in 4% PFA prior to microCT analysis ...
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bioRxiv - Developmental Biology 2020Quote: ... zebrafish were euthanized in 0.2% Tricaine (MS-222, Sigma E10521) solution ...
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bioRxiv - Developmental Biology 2022Quote: Zebrafish embryos and larvae were treated with phenylthiourea (PTU; Sigma) from 24 hpf onwards in order to induce hypothyroid conditions (Elsalini and Rohr ...
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bioRxiv - Developmental Biology 2022Quote: ... Zebrafish were sacrificed by immersing in 0.02% MS222 (Sigma, E10505) until no opercular movement ...
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bioRxiv - Neuroscience 2020Quote: ... zebrafish embryos were terminally anaesthetised in MS222 tricaine (Sigma-Aldrich) and incubated ...
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bioRxiv - Immunology 2022Quote: ... Frozen zebrafish adult brains were lysed in RIPA buffer (Sigma), 1mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Developmental Biology 2021Quote: ... Zebrafish were anesthetized using 0.0168% buffered tricaine (MS-222, Sigma). AB strains of zebrafish were used as wild-type for this study ...
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bioRxiv - Genetics 2020Quote: The zebrafish were euthanized in tricaine (MS-222, Sigma-Aldrich) and fixed for 3 days in 4% paraformaldehyde at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... zebrafish larvae were anaesthetised with 0.017% MS-222 (Sigma-Aldrich). For total fluorescence acquisition ...
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bioRxiv - Microbiology 2020Quote: Zebrafish were euthanized with tricaine (MS-222) (Sigma-Aldrich #E10521) at 0.3 mg/mL for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... zebrafish were euthanized with tricaine (MS-222, Sigma-Aldrich E10521) at 0.3 mg/mL ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish embryos were treated with 10 μM DMH1 (Sigma Aldrich) or 100 μM cyclopamine (Cayman Chemical ...
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bioRxiv - Cancer Biology 2022Quote: ... fixed zebrafish larvae firstly treated with acetone (650501, Sigma-Aldrich) at −20 °C for 7 min ...
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bioRxiv - Genetics 2022Quote: ... stained zebrafish larvae were anesthetized in 0.01% MS-222 (Sigma) and mounted into borosilicate glass capillaries using 0.75% low melt-agarose (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Zebrafish embryos were fixed overnight in 4% PFA (47608, Sigma) in PBS (EC-A500 ...
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bioRxiv - Microbiology 2023Quote: Zebrafish embryos were anesthetized using 0.04% Tricaine (Sigma-Aldrich, E10521) for experimental procedures ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Zebrafish embryos were homogenised by addition of Trizol (Sigma, USA) and passed through a QIAshredder column (Qiagen ...
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bioRxiv - Developmental Biology 2024Quote: Adult zebrafish were euthanized by immersion in 0.16% tricaine (Sigma) and hearts dissected as described53 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were diluted to 1:500 for α-Beta Tubulin (Millipore, ab9354) and α-TOMM20 (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-beta-actin antibody (A2228) purchased from Sigma-Aldrich (1:5000 dilution) were used in this study.
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bioRxiv - Molecular Biology 2020Quote: ... The antibodies used for western blot were beta-Actin (Sigma A5441, 1:2000), Cas9 (Cell Signaling 1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... The density of signal was normalized to beta-tubulin levels (1:50,000, Sigma). Translin antibody was produced (New England Peptide ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-beta-actin antibody (A2228, WB: 1:10,000) was purchased from Sigma-Aldrich. Anti-p62/SQSTM1 (PM045 ...
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bioRxiv - Neuroscience 2022Quote: ... and beta-actin (1:5000; mouse-monocolonal, Sigma Aldrich catalog #A5316 clone AC74), and a goat anti-mouse IgG secondary antibody (1:5000 ...
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bioRxiv - Microbiology 2024Quote: ... beta-tubulin I and II (cilia; 1/800; T8535, Sigma-Aldrich, MO, USA), CC16 (Club cell ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and and CD44 (SAB1405590) (mouse) at 1:400 dilution for zebrafish tissue and 1:50 dilution for human tissue (Sigma Aldrich). The following secondary antibodies were used ...
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bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Immunology 2024Quote: ... The recombinant proteins were then purified using Bugbuster reagent and His·Bind purification kit (Novagen) following the manufacturer’s user protocol ...
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bioRxiv - Microbiology 2024Quote: ... recombinant CA protein (3 mg/mL) with 500 μM or 10 mM IP6 (Sigma), 50 mM MES ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant proteins were produced from E.coli BL21(DE3) Rosetta bacteria (Sigma Aldrich, cat. 71403). Bacterial cells were grown in an LB medium for 16 hours at 25°C ...
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bioRxiv - Genetics 2024Quote: ... and 1000 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107)) supplemented with 1uM PD0325901 and 3uM of CHIR99021 (Selleck Chemicals ...
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bioRxiv - Microbiology 2022Quote: ... SasG-His was then re-purified using HIS-Select resin (Sigma) and eluted with bind buffer containing increasing concentrations of imidazole ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...