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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for Recombinant Zebrafish IL 1 beta Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: The wild-type and stat3 mutant zebrafish was euthanized with tricaine methanesulfonate (MS222, Sigma) and fixed using 2.5% glutaraldehyde (diluted in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... All zebrafish embryos utilized for microscopy were supplemented with 0.003% PTU (Sigma-Aldrich, P7629) in E3 medium (5 mM NaCl ...
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bioRxiv - Immunology 2023Quote: Zebrafish embryos at 24 hpf were treated with 5 μM 4-OHT (H7904, Sigma) for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Zebrafish Gpx4 activity was measured of the supernatant according to manufacturer’s protocol (Millipore Sigma). Total protein was measured using DC protein assay (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... zebrafish embryos were anesthetized with egg water/0.16 mg/ml tricaine (Sigma-Aldrich, A5040) and embedded in 0.7% low melting agarose ...
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bioRxiv - Genetics 2024Quote: ... 20 healthy and fecund AB male zebrafish were exposed to ENU (Millipore-Sigma, N3385) prepared at 3.3 mM in 10mM sodium phosphate buffer pH 6.5 in system water for 1 hour in a dark ...
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bioRxiv - Genetics 2024Quote: ... 20 healthy and fecund AB male zebrafish were exposed to ENU (Millipore-Sigma, N3385) prepared at 3.3 mM in 10mM sodium phosphate buffer pH 6.5 in system water for 1 hour in a dark ...
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bioRxiv - Cancer Biology 2024Quote: ... HA-tag was stained using either rabbit and HA-tag from Sigma-Aldrich (catalogue no. H6908) or mouse anti-HA-Tag (6E2 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... HA-tag (Sigma-Aldrich# H-9658), FLAG (Sigma-Aldrich#) ...
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bioRxiv - Developmental Biology 2020Quote: The Apop-Tag Fluorescein Kit (Millipore) was used for TUNEL staining according to manufacturer’s instruction with minor modifications ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (HA.C5, Millipore 05-904) or FLAG-tag (M2 ...
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bioRxiv - Cell Biology 2021Quote: ... or FLAG-tag (M2, Sigma, F3165) at 4°C ...
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bioRxiv - Physiology 2021Quote: ... the FLAG tag (F1804, Sigma-Aldrich), and Fam64a ...
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bioRxiv - Plant Biology 2021Quote: ... HA- or Myc-tags (Sigma-Aldrich) followed by staining with secondary antibodies coupled to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-HA tag (Clone 6E2, Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... anti-Myc Tag (Millipore, 05-724), anti-mouse-HRP (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... FLAG-tag (clone M2, SIGMA-ALDRICH), FLAG-tag (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... myc-tag and GAPDH (Sigma-Aldrich); S6K1 (GenScript) ...
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bioRxiv - Cell Biology 2022Quote: ... FLAG eptitope tag (M2, Sigma, #F3165), GFP (JL-8 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-S-Tag (EMD Millipore); Mouse anti-GAPDH (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG epitope tag (L00018; Sigma), Rabbit polyclonal anti-SARS-CoV-2 nucleoprotein N protein (40068-RP01 ...
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bioRxiv - Cancer Biology 2023Quote: ... FLAG tag (Sigma-Aldrich, cat F3165), β-actin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... HA tag (HRP coupled, Sigma; H6533), β-actin (HRP coupled ...
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bioRxiv - Microbiology 2024Quote: ... HA-tag (Sigma-Aldrich, Cat.#H6908), L1CAM (Santa Cruz ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Cell Biology 2021Quote: ... and a western blot was performed using anti-HIS (Sigma H-1029, mouse, 1:3000) and anti-HA (Biolegend MMS-101R or ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was incubated for 1 hour with HIS-Select® Nickel Affinity Gel (Sigma), 300 μL of gel per 500 mL of His6-SUMO-AGARP expression cell culture ...
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bioRxiv - Microbiology 2020Quote: ... Purified proteins were mixed as indicated in the figure and rotated with either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The unbound portion of this capture step was kept as HIS-free eGFP protein and conjugates and concentrated once on a 10K Amicon column (Millipore Sigma) by spinning for 6-8 minutes at 6000x g ...
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Microbiology 2024Quote: ... CrsL-His proteins were loaded in duplicate and resolved on SDS-PAGE gels and blotted onto Amersham Protran Nitrocellulose Membrane (Sigma-Aldrich). The membranes were incubated with decreasing concentrations of guanidine-HCl (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... mAb anti-α-tubulin (T6199; IF: 1/4000) and pAb anti-Flag-tag (F7425; IF: 1/500, WB: 1/1000) were from Sigma-Aldrich, and a pAb anti-GFP (632592 ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...
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bioRxiv - Neuroscience 2022Quote: ... a second set of tissue from each rat was incubated in a cocktail of rabbit anti-cFos (1:1000) and a mouse monoclonal antiserum against dopamine beta-hydroxylase (DbH; 1:5000; Millipore, MAB308; AB_2245740), followed by a cocktail of Cy3-conjugated donkey anti-rabbit IgG (red ...
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bioRxiv - Microbiology 2024Quote: ... zebrafish larvae were injected at 2 dpf with a 1:5 dilution of LC in PBS + 2% phenol red (Millipore Sigma, Cat # P0290) in the caudal vein ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Antibodies were used at a dilution of 1:5000 for anti-beta-tubulin (T8328-200UL, Sigma-Aldrich, mouse), 1:25000 for anti-GAPDH (G8795 ...
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bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
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bioRxiv - Biophysics 2020Quote: The recombinant human Mdm2 (1-125 aa) were cloned into pET11a vector (Novagen) and expressed in E ...
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bioRxiv - Cancer Biology 2020Quote: ... JSH23 (Cat# 481408) and Recombinant human IGF-1 (Cat# GF138) purchased from Millipore; JQ1 (Cat# M2167 ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10 ng/ml of human rIGF1 (recombinant Insulin growth factor-1) (Sigma, USA), 10 ng/ml amphotericin B ...
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bioRxiv - Bioengineering 2024Quote: ... Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma), prepared according to manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Approximately 5 mg of extract containing the corresponding recombinant protein was incubated with 25 µl of glutathione sepharose (SIGMA) and subsequently mixed with yeast extracts expressing Sen1 TAP-Nter (typically 0.5 mg of extract per binding reaction) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...