Labshake search
Citations for Millipore Sigma :
7701 - 7750 of 10000+ citations for Recombinant Mouse TNFRSF9 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich) and purified with a Superdex 200 pg (Cytiva ...
-
bioRxiv - Neuroscience 2020Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
-
bioRxiv - Microbiology 2021Quote: ... or thrombin protease (10 U/mg of fusion protein, Sigma-Aldrich, Germany). Importin β1 ...
-
bioRxiv - Microbiology 2021Quote: Immunoprecipitation was conducted using PureProteomeTM Protein A/G Mix Magnetic Beads (Millipore). Briefly ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
-
bioRxiv - Developmental Biology 2022Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
-
bioRxiv - Genomics 2022Quote: Fusion protein overexpression was induced by treatment with doxycycline (dox) (Sigma Aldrich) at a concentration of 1µg/mL for the specified amount of time ...
-
bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
-
bioRxiv - Neuroscience 2022Quote: ... protein content was additionally measured using the Bradford assay (Sigma-Aldrich, USA). Bovine serum albumin dissolved in 1xPBS was used for the standard curve ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... mice received either 5 nmol Endo Grade® Ovalbumin protein (Sigma-Aldrich) alone ...
-
bioRxiv - Immunology 2022Quote: ... proteins were transferred to a methanol-activated PVDF-FL membrane (Millipore Sigma) in NuPAGE transfer buffer (Invitrogen ...
-
bioRxiv - Bioengineering 2022Quote: ... and fibrinogen from bovine plasma (Type I-S, ≥75% clottable protein, Sigma). A 6-8 wt% gelatin stock solution was made by dissolving lyophilized gelatin in calcium and magnesium-free phosphate-buffered saline (10 mM PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto methanol-activated Immobilon-P PVDF membranes (Merk Millipore) using a Mini-Transblot Cell (Bio-Rad) ...
-
bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated using Amicon concentrators (100-kDa cutoff; Millipore), aliquoted ...
-
bioRxiv - Biochemistry 2020Quote: ... The purified proteins were concentrated using Amicon concentrators (100-kDa cutoff; Millipore), aliquoted ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The culture supernatant was loaded onto a protein A-sepharose column (Millipore), and the bound antibody was eluted by the addition of 0.2 M glycine–HCl (pH 2.7) ...
-
bioRxiv - Microbiology 2020Quote: ... Complexes were pulled down using Dynabeads Protein A/G (Sigma-Aldrich, 14321D) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein was then transferred to a 0.45um Immobilon-P PVDF membrane (Millipore). After blocking for 1 hr in 5% nonfat dried milk in TBST ...
-
bioRxiv - Molecular Biology 2021Quote: ... The protein activity was measured by an ATPase activity kit (Sigma-Aldrich) as described by the manufacturer.
-
bioRxiv - Molecular Biology 2020Quote: ... TAP tagged proteins was captured using IgG sepharose fast flow beads (Sigma) and proceeded as described (33) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The separated proteins were transferred to a PVDF membrane (Millipore, 0.2 μm). The membrane was blocked in 5% nonfat dry milk (Biofroxx ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were detected with antibodies of γ-H2AX (Cat# 05-636, Millipore) and β-actin (Cat# 4967 ...
-
bioRxiv - Neuroscience 2020Quote: ... anti-MBP (Myelin Basic Protein, rat polyclonal, 1:1000 Millipore MAB 386) or antimouse IgG (Biotin-SP AffiniPure ...
-
bioRxiv - Cell Biology 2021Quote: ... the proteins were electroeluted from gels onto Immobilon-F PVDF membranes (Millipore) at 100 mA for 16-18 hr at 4°C under wet blotting conditions (10 mM 3-[Cyclohexylamino]-1-propanesulfonic acid pH 11.0 ...
-
bioRxiv - Physiology 2021Quote: ... muscle whole-cell protein lysates were diluted in MPER buffer (Sigma-Aldrich) supplemented with protease and phosphatase inhibitor cocktails ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... BAL cytokines were profiled using a pre-mixed MILLIPLEX protein immunoassay (Millipore) that was read on a Bio-Plex 200 multiplex suspension array system (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... after which the proteins were transferred to Immobilon P membranes (Merck Millipore). After blocking the membrane with 5% skimmed milk in TBS-T buffer solution for 1 h at RT or overnight at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... purified Rv1019 protein (200μg) was emulsified in TitreMax Gold adjuvant (Sigma-Aldrich) and used to immunize two one-year old male rabbits by subcutaneous injection ...
-
bioRxiv - Microbiology 2020Quote: ... Fractions containing the desired protein were concentrated (Amicon Ultracel 100 K, Millipore) and further purified by size-exclusion chromatography (SEC ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were transferred onto an Immobilon P membrane (Millipore, Burlington, Massachusetts, USA) using TRANS-BLOT SD SEMI DRY TRANSFER CELL (Bio-Rad ...
-
bioRxiv - Microbiology 2021Quote: ... The LAMP1 protein was detected with rabbit anti-LAMP1 (Sigma, Cat# SAB3500285) and horseradish peroxidase-conjugated mouse anti-rabbit (Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... the protein was overexpressed in Rosetta (DE3) pLysS E.coli cells (Millipore Sigma) and grown in Terrific Broth (TB ...
-
bioRxiv - Developmental Biology 2020Quote: ... We used 10µl G-protein coupled Sepharose beads per IP (SIGMA, P3296). Anti-Myc IPs were performed with 10µl Anti-Myc-Agarose bead (SIGMA ...
-
bioRxiv - Microbiology 2020Quote: ... and the resulting protein was concentrated by Amicon Ultra centrifugal filter (Millipore). The sample was diluted to 2.5 μg/ml and analyzed on a glycan 100 binding array provided by Creative Biochip ...
-
bioRxiv - Pathology 2020Quote: Dissolve proteins and FITCs in FluoroTag™ FITC Conjugation Kit(Sigma-Aldrich)kit buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The IMAC fractions containing the protein were concentrated by ultrafiltration (Amicon, Millipore) and then injected onto a Superdex 200 10/300 FPLC column (GE Healthcare ...
-
bioRxiv - Physiology 2020Quote: ... and protein bands were detected using Ponceau S staining solution (Sigma, P7170).
-
bioRxiv - Biochemistry 2020Quote: ... 2.5 µl 5000x SPYRO Orange protein gel stain (Sigma-Aldrich catalog #S5692) to 1977.5 µl of buffer (20 mM MOPS ...
-
bioRxiv - Biochemistry 2021Quote: The solubilized protein was then loaded on a DEAE CL-6B (Sigma) column ...
-
bioRxiv - Biochemistry 2021Quote: ... The protein was applied to column packed with ATP-agarose (Sigma Aldrich) and the column was washed with buffer A (25 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were transferred to low fluorescence PVDF membrane (Millpore Sigma cat. # IPFL00010). REVERT total protein stain was used to detect and quantify transferred proteins ...
-
bioRxiv - Biochemistry 2021Quote: ... and equal amount of protein was subjected to benzonase nuclease (Millipore Sigma) treatment ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
-
Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... The separated proteins were transferred onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) using phosphate-based transfer buffer (10 mM sodium phosphate monobasic ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
-
bioRxiv - Physiology 2021Quote: ... The protein peak was collected and 20μM of iodoacetamide (Sigma-Aldrich; I1149) was added to the solution ...
-
bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...