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Citations for Millipore Sigma :
7601 - 7650 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 3.5 × 105 cells were seeded in 24-well plates on coverslips and treated with 10 nM PMA (Sigma) overnight to induce adherence ...
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bioRxiv - Cell Biology 2023Quote: CR expanded HBE cells were seeded on collagen I coated 24-well Transwell membranes (Corning, USA; 6.5mm 0.4µm pore polyester membrane Sigma CLS3470) at a density of 1.5×105 cells/insert ...
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bioRxiv - Bioengineering 2023Quote: ... we pre-treated 48-well plastic plates with 200 μL of 20 μg/mL human fibronectin (Milipore Sigma) for 10 minutes at 37 °C under 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... cells in 24-well plates were transfected as described above and then fixed using 4% paraformaldehyde (Sigma-Aldrich) in 1x phosphate-buffered saline (PBS 10x Powder Concentrate ...
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bioRxiv - Cell Biology 2023Quote: ... 4 x 105 cells per well were plated in media containing 100 nM GSI (Compound E; Millipore, 565790) on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were transferred to 384-well plates pre-coated in-house with anti-FLAG antibodies (Sigma-Aldrich, F1804). Lysates were incubated at 4 °C for 3 hours with gentle rocking and then washed six times in cold HENG buffer (50 mM HEPES-KOH ...
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bioRxiv - Cell Biology 2023Quote: ... 4⨉106 cells were seeded into each well of a collagen (type I from calf skin - Millipore Sigma)-coated 12-well plate and grown at 37 ℃ for 24 h in a CO2-jacketed incubator by which they reached 100% confluence ...
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bioRxiv - Cancer Biology 2023Quote: ... EBs were differentiated using a differentiation medium (1 ml/well) initially supplemented with 1µg/ml of Doxycycline (Sigma) for five days ...
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bioRxiv - Neuroscience 2024Quote: ... cells were plated in 48-well plates pre-treated with 50 mg/mL human fibronectin (FC010-10MG, Millipore) and incubated for 24 hr ...
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bioRxiv - Molecular Biology 2024Quote: Transfected cells from 24-well plates were lysed in RIPA buffer containing 1X protease cOmplete inhibitors (#11836145001, Sigma) and quantified using BCA method (BCA Protein Assay ...
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bioRxiv - Molecular Biology 2024Quote: ... extracellular Cryptococcus was washed off and wells were treated with 10 μg/mL calcofluor white (CFW) (Sigma-Aldrich) for 10 min at 37°C to distinguish between phagocytosed and extracellular C ...
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bioRxiv - Cell Biology 2024Quote: ... as well as primary anti-tyrosinated tubulin antibody from mouse (monoclonal TUB-1A2, T9028 Sigma, 1/5000 dilution) along with mouse anti-GAPDH antibody (monoclonal 60004-1-Ig ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Each well had one fish placed in 450 μl of fish water without or with PTZ (Sigma-Aldrich). 60 zebrafish larvae were used in total ...
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bioRxiv - Cancer Biology 2024Quote: CDK12-KO 22Rv1 and AAVS1 control cells were seeded into four-well Millicell EZ slide (Millipore, Cat. PEZGS0416) pre-coated with Poly-L-Lysine (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... before being fixed with 1 ml per well of 0.5% crystal violet staining solution (0.5 g crystal violet powder [Milipore Sigma C0775] ...
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bioRxiv - Physiology 2024Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
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bioRxiv - Microbiology 2024Quote: ... the culture medium was removed and switched to either 80 μL/well of Alkaline (RPMI 1640 (Sigma, #R6504) containing 1% FBS and 50 mM HEPES (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% ascorbic acid was added to a sterile saline solution (NaCl, 0.9% w/v) and 6-OHDA HCl powder (6-hydroxydopamine hydrochloride from Sigma or 6-OHDA hydrobromide from Tocris) was then dissolved to produce a final 6-OHDA concentration of 4.44 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... for 3 min and then placed in a solution of 1% w/v Alcian blue in 3% acetic acid (pH 2.5) for 30 min (EMD Millipore, Cat. No. TMS-010-C). Slides were then rinsed in 3% acetic acid and allowed to dry overnight ...
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bioRxiv - Plant Biology 2020Quote: ... 1,000-3,500 primary roots/sample were cut ~0.5 cm from the root tip and placed in a 35 mm-diameter dish containing a 70 μm cell strainer and 4.5 mL enzyme solution (1.25% [w/v] cellulase [ONOZUKA R-10, Yakult], 0.1% Pectolyase [P-3026, Sigma], 0.4 M mannitol, 20 mM MES (pH 5.7), 20 mM KCl ...
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bioRxiv - Bioengineering 2021Quote: ... and mixed with an equal volume of 0.01% (w/v) solution of one or more dyes (all from Sigma Aldrich, except calcofluor white from Megazyme). Cells were imaged with a 40x or 60x objectives on a laser scanning confocal microscope (Carl Zeiss ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were immediately snap-chilled by mixing with 25% w/v of crushed ice and 37% formaldehyde (solution (methanol-stabilised solution; ‘formalin’; Merck/Sigma-Aldrich cat. no. F11635-500ML) was added to a final concentration of 2.2% w/v immediately under constant mixing ...
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bioRxiv - Molecular Biology 2024Quote: ... or IL6 plus SC144 for 96h were stained with 5 uL Annexin V-FITC and 5 μL Propidium iodide (PI, Sigma-Aldrich, St. Louis, MO, USA) and analyzed by flow cytometry (LSR II ...
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bioRxiv - Genomics 2022Quote: ... in a non-stick centrifuge tube, and then 220 μl of freshly prepared PEG 4000 solution (40%, w/v) (Catalog No. 807490, Sigma-Aldrich Biotech, St. Louis, MO) was added to mediate transfection in the dark ...
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bioRxiv - Plant Biology 2023Quote: ... the samples were trimmed down to a 3-5 mm region around the area of interest and embedded in 5% (w/v) agarose (A9539; Sigma-Aldrich, St. Louis, MO, USA) in deionized water ...
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bioRxiv - Cancer Biology 2023Quote: ... while cells migrated to the lower surface of the inserts were stained with 0.5% Crystal Violet solution (50% Crystal Violet 1% Sigma V-5265, 35% ethanol in water). Four images of each insert were acquired at EVOS microscope and analyzed with the ImageJ Software to estimate the occupied area and calculate migration rate.
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bioRxiv - Microbiology 2023Quote: ... Membranes were then gently washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) and incubated in Alexa Fluor 680 Donkey anti-goat secondary (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Two different tracer solutions were prepared by diluting a 0.1% w/v stock solution of 70 kDa or 500 kDa FITC-dextran (both Sigma-Aldrich, FD70S-100MG and FD500S-100MG) 1:1000 in DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were dissected into fragments approximately 9 mm3 in size and placed in glyoxal acidic solution (3% w/v glyoxal [Millipore Sigma 128465], 20% ethanol [Fisher BP2818100], 0.75% acetic acid [Millipore Sigma A6283], 5mM NaOH [Millipore Sigma 72068], in ddH2O) for 15 min at 4C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested 24 hours after transfection and lysed in RIPA buffer (150 mM Tris-HCl [Sigma-Aldrich], 150 mM NaCl [Sigma-Aldrich], 0.5% (w/v) sodium deoxycholate [Merck Life Science BV] ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed twice with PBS and lysed in RIPA buffer (150 mM Tris-HCl [Sigma-Aldrich], 150 mM NaCl [Sigma-Aldrich], 0.5% (w/v) sodium deoxycholate ...
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bioRxiv - Genetics 2020Quote: Pheromone components (bombykol and bombykal, 96% purity, purchased from Nimrod Inc., Changzhou, China) were diluted in hexane (98% purity, Sigma-Aldrich Co., St. Louis, MO, USA) and a dose of 10 µg was used for each trial as previously described [43] ...
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bioRxiv - Biophysics 2020Quote: A polyethylene glycol (PEG) solution (w/v, 6%) was made by dissolving PEG powder (Molecular weight 300,000; Sigma-Aldrich Co Ltd, MO, US; product of USA) in Milli-Q water at 50 °C with magnetic stirring for 8 h (25) ...
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bioRxiv - Biochemistry 2023Quote: Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit FITC (Cat. No. 88-8005-72) was used in combination with Propidium Iodide Solution (Sigma-Aldrich, Cat. No. P-4864-10mL) as described in manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... For immunoprecipitation PNS were incubated with Anti-V5 Agarose Affinity Gel conjugated beads or with Protein-A beads (Sigma, 1:10 w/v swollen in PBS) and anti-HA antibody for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: Sulfolobus acidocaldarius MW001 was grown in Brock medium (Brock et al., 1972) supplemented with 0.1% (w/v) N-Z amine (Sigma-Aldrich®, Merk KGaA, Burlington, MA, USA), 0.2% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 cells were plated in 6-well poly (2-hydroxyethyl methacrylate) (poly-HEMA, Sigma-Aldrich, St. Louis, MO, USA)-coated plates for the indicated amount of time in the figure legends ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24-well plates at concentration of 15,000 cells/well with glass coverslips previously coated with 0.2% Gelatin in sterile water (G1393, Sigma-Aldrich). Both type of cultures ...
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bioRxiv - Developmental Biology 2021Quote: ... and were seeded on 6-well plates and on 13 mm circular glass coverslips (Hecht, cat no. 6.071 724) in 12-well plates coated with 0.1 % porcine gelatin (Sigma-Aldrich) at densities of 6.25 × 104 cells/cm2 for cells to be harvested at days 0 and 1 of differentiation ...
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bioRxiv - Immunology 2021Quote: ... with colorimetric signals developed with 100 μL/well of 3,3ʹ,5,5ʹ-Tetramethylbenzidine (TMB) Liquid Substrate (Cat # T0440, Sigma-Aldrich) and stopped using 50 μL/well of 3M HCl ...
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bioRxiv - Biophysics 2020Quote: ... Cytochalasin D (from Zygosporium masonii) as well as fetal calf serum (FCS) were from Sigma Aldrich (Merck KGaA, Germany). DPPC (1,2-dipalmitoyl-sn-glycero-3-phosphocholine) ...
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bioRxiv - Microbiology 2022Quote: ... the larvae were kept in 6-well plates containing egg water (60 μg/mL sea salts (Sigma-Aldrich; S9883) in demi water ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells (106 cells/well) were treated with 50 ng/ml phorbol-12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) for 48 h to induce differentiation into macrophage-like-cells before infection ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated on glass coverslips coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Neuroscience 2021Quote: ... in 16-well stripes (500,000 cells/well) and re-plated in plastic dish (Costar) coated with 100 μg/ml Poly-L-Lysine (Sigma) and 1 μg/ml Laminin (Sigma) ...
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bioRxiv - Genomics 2019Quote: ... 40 nuclei were sorted per well into eight 96-well plates (total of 768 wells) containing 10.5 µL EB (25 pmol primer i7, 25 pmol primer i5, 200 ng BSA (Sigma). Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter) ...
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bioRxiv - Synthetic Biology 2019Quote: ... we grew the cells in plastic 48-well plates that were pretreated with 50 μg/mL human fibronectin (Millipore) for at least 10 min at 37 °C before cell plating (to improve cell adherence) ...