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Citations for Millipore Sigma :
7451 - 7500 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 3 ×104 THP-1 cells were seeded and differentiated by the addition of 5 ng/ml PMA (Sigma) in complete RPMI media (Himedia ...
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bioRxiv - Biochemistry 2022Quote: ... The final supernatant was concentrated to > 1 mg/ml using a 3 kDa centrifugal filter unit (Millipore Sigma) and frozen in aliquots stored at −80°C ...
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bioRxiv - Bioengineering 2023Quote: ... for 20 min at room temperature and then washed 3 times in Phosphate-buffered saline (PBS, Sigma-Aldrich) solution ...
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bioRxiv - Immunology 2023Quote: ... Regeneration down to immobilized sdab026 were achieved with 3×15 second pulses of 10 mm glycine (Sigma Aldrich) at pH 1,5 ...
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bioRxiv - Bioengineering 2023Quote: Monocytes were activated to macrophages by adding 30ng/mL phorbol-12-myristate-3-acetate (PMA; Sigma-Aldrich: P8139) for 24hr ...
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bioRxiv - Cell Biology 2022Quote: ... Embryos were dechorionated in 3% sodium hypochlorite and immersed in Halocarbon oil 700 (Sigma Aldrich, 9002-83-9). Then the embryos were imaged after stage 15 when the tracheal tree formed at 3 hours intervals ...
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bioRxiv - Biochemistry 2022Quote: Whole FBS (15 mL) was passed through an Amicon Ultra 3 kDa MW cutoff centrifugal filter (#UFC900308, Millipore) and the resulting ∼15 mL that passed through the filter (corresponding to <3 kDa FBS material ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were counterstained after 3 washes of PBS with 0.3 μg/mL 4,6-diamidino-2-phenylindole (Sigma-Aldrich). Images including Z-stacks were acquired on a Leica SP8 gSTED with an objective with x63 magnification ...
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bioRxiv - Cell Biology 2023Quote: ... Desalted siRNA duplexes modified by double-deoxythymidine overhangs at 3’-ends of both strands were synthesized by Sigma. The AllStar siRNA (QIAGEN ...
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bioRxiv - Developmental Biology 2022Quote: ... Filtered medium was concentrated using Amicon Ultra-15 Centrifugal Filter Unit (3 kDa cut-off; Millipore, cat. # UFC900308) at 5,000 g for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM BME) and concentrated to <4 ml using 100,000 kDa MWCO Amicon® Ultra spin concentrators (Millipore). Protein was loaded on a Superdex 200 pg 16/60 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with 1× PBS and blocked in 3% BSA with 0.1% Triton-X 100 (Thermo-Fischer) and 0.1% Tween (Sigma) in PBS for one hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The column was eluted using 6 mL of Strep-start buffer supplemented with 3 mM d-Desthiobiotin (Sigma). The elution was diluted with 20 mL of ion-exchange buffer (25 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Avanti) and 0.08 mol% Atto488 labeled DPPE (1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine labeled with Atto488, Sigma-Aldrich) with an argon stream followed by desiccation for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... Cell viability was determined after 72 hours using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (#M5655, Sigma) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and blocked for 1h with 3% goat serum and 1% BSA in PBS/0.25% Triton X-100 (Sigma). The primary antibodies were incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated for 2 hours with 3 µg/ml 5-bromo-2’-deoxyurine (Sigma-Aldrich, B5002). Cells were then detached using trypsin/EDTA (GIBCO ...
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bioRxiv - Developmental Biology 2023Quote: ... Patch pipettes (3-5 mΩ) were filled with potassium gluconate internal solution (130 mM K-gluconate (Sigma-Aldrich), 11.5 mM Na2-phosphocreatine (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... and blocked for 1 hour at RT in 3% BSA (Applichem) in PBS-Tween buffer (0.01%) (ThermoFisher, Sigma). The membranes were then incubated overnight at 4°C with primary antibodies listed in the Supplemental table 3 with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... The MTT [3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyl-tetrazolium Bromide] dye (M2128) was obtained from Sigma Aldrich. Triple distilled water (Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Samples were prepared in fully deuterated 400 mM SPB for all measurements and contained ∼1 mM 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid (Sigma-Aldrich) as a frequency reference.
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bioRxiv - Cell Biology 2023Quote: ... Cells (or fibers) were then blocked with 3% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, MO, USA) diluted in PBST for 1 hr at room temperature to block non-specific binding sites ...
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bioRxiv - Developmental Biology 2023Quote: ... the L1 larvae were transferred to NGM+auxin plates with 1 mM 3-indoleacetic acid (IAA; Sigma #I2886) and kept in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times and 100 µL per well of SIGMAFAST OPD tablet (#P9187, Sigma-Aldrich, USA) solution was added ...
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bioRxiv - Biochemistry 2023Quote: ... a 3-30% continuous gradient was prepared using Optiprep density gradient medium of 60% iodixanol solution (Sigma-Aldrich) in a buffer composed of 78mM KCl ...
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bioRxiv - Cancer Biology 2023Quote: ... the input media were centrifuged at 3,200 g for 30 min with 3 kDa MWCO filter units (Millipore); after centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... membranes spotted with different lipid species were blocked with 3% fatty acid free BSA (w/v; Sigma, 7030) in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then blocked in 0.45-μm filtered 3% (w/v) bovine serum albumin (BSA; Sigma-Aldrich A7906) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Ovarian cells were isolated by enzymatic digestion in 3 mL of Dulbecco’s Modified Eagle’s Medium (DMEM) (cat# D6429, Sigma) containing 4 mg collagenase (cat# C5138-100MG ...
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bioRxiv - Genetics 2023Quote: ... at 37 °C on a heating plate with 10µL digestion mix (3% [wt/vol] pectolyase Y-23 [MPBiomedicals], 0.3% [wt/vol] Driselase [Sigma] 0.3% [wt/vol] cellulase [OnozukaR10] [Duchefa] 0.1% sodium azide in 10 mM citrate buffer) ...
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bioRxiv - Genomics 2023Quote: ... Young adult worms were harvested into 100∼200 μL M9 buffer and mixed with equal volume dyes (DiI, 1,1’-dioctadecyl-3,3,3’,3’,-tetramethylindo-carbocyanine perchlorate, Sigma, 468495) at the working concentration (20 μg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the cells were washed 3 times in PBS before addition of 2% Triton X– 100 (Sigma-Aldrich) PBS solution to lyse the cells for enumeration of the intracellular bacteria ...
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bioRxiv - Cell Biology 2023Quote: ... Imidazole was removed by 3 cycles of concentration and dilution using Vivaspin (Millipore Sigma, Cat# GE28-9322-25). TEV enzyme was added at about 1/10 of the target protein mass and incubated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... was washed with the IP lysis buffer and incubated with 3 ug anti-Flag antibody (Sigma, M2, F1804) for 1 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... antisense strand: 5’-GUA UCU CUU CAU AGC CUU A(dT)(dT)-3’) were synthesized by Sigma Aldrich. Early Endosome Antigen 1 (EEA1 ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed in PBS and permeabilised in PBS supplemented with 3% FBS and 0.1% Saponin (Sigma-Aldrich) for 5 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: The following chemicals were used in this study: indole-3-acetic acid (IAA, auxin) (87-51-4) (Millipore); 4-hydroxytamoxifen (4-OHT ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant containing the pseudotyped lentiviral particles was harvested 2 to 3 days after transfection and filtered through 0.45μm CA membranes (Millipore). For transduction ...
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bioRxiv - Developmental Biology 2024Quote: ... Stained zebrafish larvae were anesthetized with 0.016% (m/v) Ethyl 3-aminobenzoate methanesulfonate salt (Tricaine; Sigma-Aldrich, A5040) and embedded in 1.8% low melting point agarose (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and collagenase type 1 was purchased from Sigma-Aldrich. RNA extraction kit (Iran) ...
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bioRxiv - Cell Biology 2024Quote: Treatments for the LC3 assay included 3 hours with mTOR inhibitor Torin1 (Merck-Millipore, final concentration 250 nM), Bafilomycin A1 (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... After 24 h media/inhibitor was aspirated and replaced with 20 μl of 5 mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma) and incubated at 37°C in 5% CO2 for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Bands were stained in 5-bromo-4-chloro-3-indolyl-phosphate and nitro blue tetrazolium solution (Sigma-Aldrich). The membrane image was acquired using a digital steel camera EOS M6 mark II with a lens EF16-35 mm F4L IS USM (Canon Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... slides were cleared in Xylene (2 × 3 min) and coverslipped with DPX mounting medium (Cat# 44581, Sigma-Aldrich). Sections were photographed with an epifluorescence microscope (Model BX51 ...
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bioRxiv - Cell Biology 2024Quote: ... The number of viable cells was quantified using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; M2128, Sigma) assay at 0 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total of 150 µL of 3% 4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (oxazolone; Sigma-Aldrich) dissolved in 100% ethanol (Wako ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was applied to 3 kDa MWCO filters (Amicon Ultra-0.5 Centrifugal Filter; Merck Millipore, Darmstadt, Germany), and centrifuged at 14,000 × g for 45 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... for 10 min at RT and then permeabilized for 3 min with 0.2% Triton-X 100 (Millipore Sigma) Over-extracted cells were treated with 0.4% Triton-X 100 ...
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bioRxiv - Cancer Biology 2024Quote: ... the PDMS stamp is placed on a silanized coverslip pretreated with 2% (3-Trimethoxysil) propyl-methacrylate (Sigma #M6514)) ...