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Citations for Millipore Sigma :
7501 - 7550 of 7633 citations for Yellow Fever Virus NS1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by standard curve intensity measurements from Coomassie blue-stained bovine serum albumin (A7617, Sigma Aldrich, St. Louis, MO, USA) of known concentrations.
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined by standard curve intensity measurements from Coomassie blue-stained bovine serum albumin (A7617, Sigma Aldrich, St. Louis, MO, USA) of known concentrations.
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bioRxiv - Developmental Biology 2024Quote: ... Puromycin incorporated into the proteins were detected in a Western blot using a mouse-origin anti-puromycin monoclonal antibody (12D10, Sigma MABE343, lot # 3484967) and a goat-anti-mouse HRP-conjugated secondary antibody (Abcam ab6789 ...
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bioRxiv - Biophysics 2024Quote: ... 10% v/v glycerol and 0.03% w/v DDM and 300 mM imidazole) and the eluted protein was concentrated to ∼500 μL using 100 kDa MWCO centrifugal filters (Millipore AmiconTM Ultra, Merck). The concentrated sample was then loaded onto a Superdex 200 10/300 gel filtration column (Cytiva ...
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bioRxiv - Genetics 2024Quote: Snap frozen gastrocnemius or spinalis muscles were homogenised in 4% SDS lysis buffer and assessed for total protein concentration using Direct Detect Assay free cards (Millipore, Cat #DDAC00010-GR). Proteins were separated by SDS-PAGE using stain-free precast midi- criterion gels (Biorad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30-60 µg protein extracts were fractionated using 8-10% SDS-PAGE under reducing conditions and transferred onto Immobilon-FL PVDF (Millipore, cat no# IPFL00010) membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Thirty µg of total protein of each sample were separated on 15% SDS–polyacrylamide gels and transferred to ImmobilonTM PDVF membranes (Millipore Co., Bedford, MA, USA). The membrane was blocked for 1 h in PBS-T with 5% non-fat dried milk at room temperature ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Primary antibodies raised against the following proteins were used in block buffer (5% BSA, 0.1% Tween-20, in PBS) at the indicated dilutions: α-Tubulin (Sigma Aldrich T5168, 1:50,000), FLAG (Agrisera AS15 2871 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The resistant populations were previously selected for high levels of resistance against protein synthesis inhibitors from the aminoglycoside family, gentamicin (GEN; Carl Roth, Germany; Ref. HN09.1) and streptomycin (STR; Sigma-Aldrich, USA; Ref. S6501-5G), or alternatively cell-wall synthesis inhibitors from the β-lactam family ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Cancer Biology 2021Quote: ... protein samples were separated on a sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto Immobilon-P PVDF membrane (EMD Millipore, Billerica, MA, USA). The membrane was incubated for 1 h in blocking buffer (Tris-buffered saline ...
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bioRxiv - Cancer Biology 2021Quote: Mitochondrial mass was assessed by measuring citrate synthase activity from 4 million whole cells normalized to protein input (U/mg) in triplicate from three biological replicates using the Citrate Synthase Assay Kit (CS0720, Sigma-Aldrich, St, Louis, MO).
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bioRxiv - Cancer Biology 2020Quote: HEK293-T cells were transiently transfected with GFP-V5 or GREP1-V5 fusion proteins using OptiMem and Fugene HD (Sigma-Aldrich, St. Louis, MO). 72 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Supernatant equivalent to ~100 mg total protein was incubated with 200 μl (packed) resin of anti-FLAG M2 affinity gel (Sigma, catalog # A2220, lot # SLCH0130) for 2 h on a rotator at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The H2O2 concentrations were determined by a standard curve developed using 0.25 – 2.5 μM and normalized by calculating the amount of protein in the extract using a standard Bradford Assay (Bradford, 1976) with Bradford Reagent (Sigma-Aldrich, cat no. B6916).
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bioRxiv - Systems Biology 2021Quote: ... Cell-free extract preparation for yeast cells was done using YeastBusterTM Protein Extraction Reagent supplemented with 1% of 100x THP solution according to the description (Novagen, San Diego, CA, USA). To a pellet with a wet weight of 0.3 g ...
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bioRxiv - Systems Biology 2020Quote: ... The pooled reference mixture that consisted of an equal amount of protein from each sample was spiked with UPS2 Proteomics Dynamic Range Standard (Sigma-Aldrich, Saint-Louis, MO) and digested with trypsin ...
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bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibody was incubated at room temperature protected from light for 1 hour in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using IRDye® 800CW Goat anti-Mouse IgG secondary antibody at 1:10,000 (LI-COR Lincoln ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was incubated at room temperature for 2 hours in Intercept®(TBS) Protein-Free Blocking Buffer with 0.01% Tween®20 (Sigma, St. Louis, MO) using STAT1 1:1000 (#14994 ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Proteins that passed this quality control test were then labeled directly with NHS-tethered Cy5 dye (Catalog No. GEPA15101, Sigma-Aldrich, St. Louis, MO) on the glutathione beads ...
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bioRxiv - Cancer Biology 2021Quote: ... Denatured proteins in the supernatant were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (Millipore Corporation, Billerica, MA, USA) membranes ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: A gBlock Gene Fragment coding for DHFR2 – αB7-H3 scFv fusion protein was ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864–3) via the NcoI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: The proteins were visualized by incubating membranes for 30 minutes in BCIP®/NBT Alkaline Phosphatase Substrate (B5655, SIGMAFAST™ BCIP®/NBT, Sigma-Aldrich). The intensities of the detected bands were analyzed with a FluorChem 8800 Imager (Alpha Innotech ...
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bioRxiv - Bioengineering 2022Quote: ... and αCD133-scFv-DHFR2 fusion proteins were ordered from Integrated DNA Technologies (IDT) and cloned into the Novagen pET28a(+) vector (EMD Millipore, Cat: 69864-3) via NcoI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The fractions containing GRP78 protein were combined and concentrated by centrifuging through 30-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore, Billerica, MA, USA). A solution of GRP78 protein was prepared at the concentration of 6 mg·ml-1 in 50 mM NaHCO3 buffer for the binding experiments.
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 3 hours with primary antibodies directed to either AGO proteins (WAKO anti-AGO2 #011-22033, EMD Millipore anti-panAGO #MABE56) or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... followed by donkey anti-rabbit DyLight405 (Jackson Immuno Research 711-475-152) or neuron nuclei protein marker (NeuN, neuronal marker, guinea pig anti-NeuN, EMD Millipore ABN90, ON, Canada) followed by donkey anti-guinea pig (CF405M ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed 3 times with PBST and the protein bands were visualized by enhancing TMB substrate solution (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2022Quote: ... neurons from frozen spinal cord sections were stained by incubation with the specific neuronal marker anti-neuronal nuclei protein (NeuN, 1:500; Merk Millipore cat#MAB377, RRID: AB_11210778) diluted in blocking solution (PBS with 0.1% BSA and 0.2% Triton X100) ...
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bioRxiv - Immunology 2022Quote: ... and then lysed for 15 min with a M-PER Mammalian protein extraction reagent (Pierce Biotechnology, Rockford, IL) containing a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO) and 1 mM p-amidinophenyl methanesulfonylfluoride (PMSF) ...
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bioRxiv - Cancer Biology 2022Quote: ... 300 ug of protein in 600 uL of lysis buffer was used for the GTP pulldown with 20 ul of PAK beads (Sigma, Cat. no. 14-325) incubated for one hour ...
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bioRxiv - Neuroscience 2019Quote: ... 50 µl dynabeads Protein G (ThermoFischer Scientific, Waltham, MA, USA) were blocked using albumin from chicken egg white (Sigma-Aldrich, St. Louis, MO, USA) while rocking for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Biophysics 2020Quote: ... The following globular proteins were used to fit a standard curve of elution profile versus hydrodynamic radius: thyroglobulin 86 Å (Sigma-Aldrich, Cat #T9145) bovine serum albumin–dimer 45.6 Å and monomer 36.2 Å (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... 20µg of protein of each sample was separated on polyacrylamide gels at 160V for 80 minutes and blotted onto PVDF-membranes (Millipore, 80mA/membrane, 90 minutes). After blocking for 2h in Tris-buffered saline with Tween-20 containing 5% nonfat dry milk and/or BSA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The purified BGLF4 was then used for kinase inhibition assays using the C-terminal fragment peptide of retinoblastoma protein (RB) as substrate (Millipore Sigma cta# 12-439). The remaining assay parameters were the same as those applied for PknB except for the following changes ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2021Quote: ... sBHI media controls or Porphyromonas cell-free supernatants (240 µg of total protein) were incubated with 120 µg of human fibrinogen (Sigma-Aldrich, St. Louis, MO). Reaction mixtures were incubated at 37°C under anaerobic conditions or in an atmosphere of 5% CO2 for cell suspension or cell-free supernatants ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the target protein were concentrated to 2 mL using an Amicon Ultra Centrifugal filter (Millipore; 30 kDa molecular weight cut-off (MWCO)) and injected into a gel filtration column (HiLoad 16/60 Superdex 200 ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed or tissue mechanically homogenised in lysis buffer (Extraction buffer, FNN0011) and protein content was quantified by a BCA assay (Sigma-Aldrich, St. Louis, MO). Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Western blots were treated with antibodies against the appropriate proteins followed by a second reaction with anti-mouse or anti-rabbit IgG conjugated to horseradish peroxidase (Sigma, St. Louis, MO, USA). The SuperSignal West Pico and Femto substrate kits (Pierce ...
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Separated proteins were transferred to polyvinylidenedifluoride membranes that were blocked in 5% non-fat milk or 5% BSA (Sigma-Aldrich, St. Louis, MO, USA) in Tris-buffered saline with 0.1% Tween 20 (TBS-T) ...