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Citations for Millipore Sigma :
7251 - 7300 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The protein solution was then concentrated using a 3 kDa cutoff centrifugal filter unit (Millipore, Burlington, MA, USA) and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2020Quote: ... a mica surface was treated for 5 min with 0.01% (v/v) (3-aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich). HS-AFM observations of the 50S subunits from Pyrococcus furiosus or pre-assembled hybrid 50S subunits were performed as follows ...
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bioRxiv - Cell Biology 2020Quote: ... 3 mM MgCl2, 0.002% gelatin, 0.4 mM dNTP mix, 0.06 unit/ml of Taq DNA Polymerase, Sigma Aldrich) and 0.5 μM of each primer ...
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bioRxiv - Cell Biology 2021Quote: ... (3) 30 μL casein wash buffer (30 mM DTT, 1 mg/mL filtered casein (Sigma-Aldrich, C5890-500G) in BRB80 pH 6.8) ...
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bioRxiv - Cell Biology 2021Quote: ... The SDS was sequestered via addition of 13 ml extraction buffer (3% Triton X-100 (Sigma-Aldrich #T8787), 50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... eggs were collected from the oviducts in MEM/polyvinylpyrrolidone media containing 3 mg/ml hyaluronidase (Sigma-Aldrich, #H3506) in MEM for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... for 3 hr to induce mCherry–FokI expression and 1 mg/ml doxycycline hyclate (D9891–G, Sigma–Aldrich) for an additional 3 hr to induce reporter gene transcription ...
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bioRxiv - Cancer Biology 2020Quote: An optimized number of cells (3 × 103 to 10 × 103) were seeded in 24-well plates (Sigma-Aldrich) and allowed to attach ...
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bioRxiv - Biochemistry 2022Quote: ... 0.02% Triton X-100 and blocked for 1 hour in a 3% Bovine Serum Albumin (BSA, Sigma Aldrich), 0.02% Triton X-100 blocking solution ...
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bioRxiv - Developmental Biology 2022Quote: ... This plasmid was transformed to Novagen’s Rosetta™ 2(DE3)pLysS competent cells (Millipore Sigma, Cat#71403-3) for rhFGF10 expression ...
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bioRxiv - Biophysics 2022Quote: ... 0.1 M NaCl final concentration) and repeated ultrafiltration against water in a 3 kDa MWCO spin-unit (Millipore). Size-defined heparan sulfate (HS-b ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Coverslips were then incubated with 200μl of primary antibody solution for 1 h at RT and washed 3 times with PBS before incubation with secondary antibody solution containing 0,1μg/mL DAPI solution (Sigma) for 1 h at RT ...
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bioRxiv - Developmental Biology 2022Quote: ... were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40: Sigma). Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866 ...
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bioRxiv - Cancer Biology 2022Quote: ... 25mM Tris) supplemented with protease and phosphatase inhibitors (Protease inhibitor cocktail, Phosphatase inhibitor cocktail 2/3, Sigma Aldrich) for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... 3 the cell culture media was replaced 1-2 hours before imaging with RPMI media (Sigma-Aldric R8755) supplemented with B27 ...
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bioRxiv - Immunology 2022Quote: ... ~7500 MDA-MB-231 cells were suspended in 50 μl of 3 mg/ml fibrinogen (Sigma Aldrich, MA) and 1.2 U/ml thrombin (Sigma Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... 650 µl of 100% ethanol and 33 µl of 3 M sodium acetate pH 5.2 (Sigma Aldrich, S7899) was added ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissue sections were then treated with 3% H2O2 and nonspecific binding was blocked with 4% BSA (A7906; Sigma) for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... polymyxin B sulfate and 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) (Sigma-Aldrich Corp., St. Louis, MO) were dissolved in water ...
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bioRxiv - Bioengineering 2022Quote: ... The activated membranes were immediately submerged in a solution of 2% bis[3-(trimethoxysilyl)propyl]amine (Sigma-Aldrich) in 1% water and isopropyl alcohol ...
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bioRxiv - Bioengineering 2022Quote: ... slides were stained in Phosphomolybdic Acid / Phosphotungstic Acid mix (Sigma-Aldrich, product number HT15-3 and HT15-2) for 7 minutes and in Aniline Blue Solution (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Silicon chips were functionalized with a 2% (3-aminopropyl) triethoxysilane (APTES, 98%, Sigma-Aldrich, St. Louis, MO, USA) solution to attach complexes to the surface by interactions with free amine groups ...
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bioRxiv - Developmental Biology 2022Quote: ... Adult zebrafish were anesthetized in standard E3 medium containing 0.4% tricaine (ethyl 3-aminobenzoate methanesulfonate salt; Sigma-Aldrich) before ventricular resection as described previously (Xiao et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... the animals were anesthetized using 0.1% Ethyl 3-aminobenzoate methanesulfonate (MS-222, Sigma-Aldrich, St Louis, MO, USA) dissolved in Holtfreter’s solution ...
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bioRxiv - Cell Biology 2022Quote: ... Strains with degradation tags were treated with 250 µM 3-indole acetic acid (Sigma-Alrich, St. Louis, MO) in DMSO and 50 mM potassium phosphate buffer ...
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bioRxiv - Cell Biology 2022Quote: The chemical (2S, 3S)-1,4-Bis-sulfanylbutane-2,3-diol (DTT, CAS No. 3483-12-3) was obtained from Sigma; it is a strong reductant with chemical formula of C4H10O2S2 and MW 154.25g/mol ...
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bioRxiv - Plant Biology 2021Quote: ... The full length coding sequence of AdACS1/2/3 and AdACO3/5 were inserted into pET-32a (Novagen) vector and then transferred into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were blocked for 60 min at room temperature in 3% donkey or goat serum (D9663, Sigma Aldrich) in 1x PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... for 3 minutes and incubated at room temperature for 30 minutes in 1% Alcian Blue 8GX (Sigma-Aldrich) in 3% acetic acid solution ...
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bioRxiv - Neuroscience 2020Quote: Cell viability was evaluated using the 3-(4,5-Dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Sigma-Aldrich), which measures mitochondrial activity in living cells ...
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bioRxiv - Neuroscience 2021Quote: ... and at 3 days in-vitro (DIV) glial proliferation was inhibited with 2 μM cytosine arabinoside (Sigma, C6645).
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bioRxiv - Physiology 2021Quote: ... Proteins were separated by 3–15% SDS-PAGE and transferred to polyvinylidene fluoride-FL (PVDF-FL, Millipore Sigma) membranes ...
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bioRxiv - Microbiology 2020Quote: ... 2020) (Vero passage 3) was kindly provided by CDC and propagated once in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: Mice were fasted for 3 h and injected with Triton WR-1339 (1 mg/g BW, Sigma-Aldrich) via the tail veins ...
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bioRxiv - Neuroscience 2021Quote: Adult mice (3-4 months old) were injected intraperitoneally (i.p.) with 100 mg/kg of tamoxifen (Sigma-Aldrich) dissolved in 10% EtOH in corn oil (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Foam formation was avoided by the addition of 3 % (v/v) antifoam 289 (Sigma-Aldrich; St. Louis, MO). Samples were withdrawn throughout the fermentation process with the purpose of evaluating the biomass and recombinant protein expression.
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bioRxiv - Neuroscience 2020Quote: ... CGNs (3 × 106 cells) and plasmids were suspended in 70 μl of Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) and exposed to decay pulses using a CUY21-edit II pulse generator (BEX ...
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bioRxiv - Immunology 2021Quote: ... Subsequently cells were washed 3 times with PBS before being treated with 2% saponin (250 µL/well) (Sigma) in distilled water and incubated for 15 min at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately1-2 μL of endotoxin-free DNA (1-3 mg/ml) diluted in PBS/0.025% Fast Green (SIGMA) was injected into the lateral ventricles of the forebrain using heat-pulled glass micropipettes (Drummond) ...
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bioRxiv - Cancer Biology 2020Quote: ... Metal-loaded polymers were then washed with L-buffer and concentrated on a 3 kDa Amicon filter (Millipore). Partially reduced antibodies were incubated with metal-loaded polymers for 90 min at RT followed by washing with W-buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...
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bioRxiv - Microbiology 2022Quote: ... LAM was resuspended at 1 mg/mL in sterile nanopure water (Direct-Q 3 UV-R, Millipore Sigma). 60-μL aliquots were stored in low-retention tubes at –80 °C and thawed immediately prior to use ...
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bioRxiv - Microbiology 2022Quote: ... stained with REVERT total protein stain (Licor Biosciences) and blocked with 3% bovine serum albumin (BSA) (Sigma-Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Microbiology 2022Quote: ... 3-OH FAs were quantified by integrating the appropriate peak on the ion chromatogram and comparing the area with an analogous deuterated internal standard (3- hydroxytetradecanoic acid, 2,2,3,4,4-d5; Sigma-Aldrich, France). The internal standard (0.5 mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were then washed in PBTD for 3×10 min and 1mg/ml DAPI (1:250, Sigma-Aldrich) was added and incubated for 10 minutes between the first and second washes ...
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Biophysics 2019Quote: Zebrafish were anaesthetized by immersion in Ethyl 3-aminobenzoate methanesulfonate (0.02% in PBS, pH 7.5, Sigma-Aldrich, A5040) until respiratory movements of the opercula stopped (approximately 5 min) ...
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bioRxiv - Neuroscience 2019Quote: Zebrafish larvae were anesthetized in 0.02% w/v ethyl 3-amino-benzoate methanesulfonic acid (MS-222; Sigma-Aldrich), placed in a glass-bottomed dish ...
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bioRxiv - Neuroscience 2019Quote: Gaboxadol (4,5,6,7-retrahydroisoxazolo[5,4-c]pyridin-3-ol hydrochloride, THIP hydrochloride; Sigma Aldrich, St. Louis, MO, USA, # T101) was dissolved in dH2O at 1 mg/ml ...