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Citations for Millipore Sigma :
7351 - 7400 of 9891 citations for SUMO2 3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... and color was developed with 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (BCIP/NBT; Sigma). Following color development ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were anesthetized with Ethyl 3-aminobenzoate methanesulfonate salt (MS-222, Tricaine; Sigma-Aldrich Corp., St. Louis, MO). All animal procedures were carried out in accordance with guidelines established by the University of Kentucky Institutional Animal Care and Use Committee and the ARVO statement on the use of animals in research.
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bioRxiv - Cell Biology 2020Quote: DNA was precipitated with 1 volume of isopropanol and 0.1 Volume of 3 M sodium acetate (pH 5.2, Sigma) at −80°C for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... the wells were activated with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (both from Sigma-Aldrich) for 20 minutes ...
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bioRxiv - Biophysics 2021Quote: ... and then subsequently concentrated to 1.5□mL using a 3□kDa molecular weight cutoff centrifugal concentrator (Millipore Sigma). 1.5□mL of a formamide based loading dye was then added to the reaction mixture ...
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bioRxiv - Immunology 2020Quote: ... Total RNA of the macrophages was extracted by the phenol/1-bromo-3-chloropropane method (Sigma-Aldrich, UK) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... SCC1 degradation was induced by addition of 500 µM auxin (indole-3-acetic acid sodium salt, Sigma-Aldrich). Newly synthesized RNA was labelled by addition of 4-Thiouridine (4SU ...
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bioRxiv - Microbiology 2020Quote: ... a 3 μm pore-size 47 mm polycarbonate filter and a 0.2 μm pore-size Sterivex unit (Millipore).
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bioRxiv - Immunology 2021Quote: ... 400 μl of clarified supernatant/ECM scrapings were loaded onto a 3 KDa spin column (Millipore Sigma UFC500396), washed with distilled water ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... was applied for 3 min followed by washing in water and counterstaining with Hematoxylin solution (Sigma-Aldrich, HHS32). Excess of Hematoxylin was removed by immersing slides in 0.25% ammonia water followed by rinsing in water ...
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bioRxiv - Cell Biology 2020Quote: ... 5 mM DTT) and eluted with Buffer A supplemented with 5 mM DTT and 3 mM Desthiobiotin (SIGMA). Peak fractions were combined and further purified via size exclusion chromatography on a Superdex 200 (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: The cantilevers were functionalized with either bovine serum albumin (BSA, 3%, 2 h, 20 °C, Sigma-Aldrich, A7906), Bis-NHS-polyethylen glycol (5 % w/w ...
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bioRxiv - Immunology 2020Quote: ... Organs were cut up into 1-3 mm3 pieces and digested in 1 ug/mL Collagenase A (Sigma) and 0.5 mg/mL Dnase I (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The fast filtration method was applied for intracellular metabolites using a 3-place EZ-FitTM Manifold (Millipore®) connected to a single vacuum that supports simultaneous filtration of three samples ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μM 1-[2-[[(Diphenylmethylene)imino]oxy]ethyl]-1,2,5,6-tetrahydro-3-pyridinecarboxylic acid hydrochloride hydrochloride (NO711) (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... Slides were blocked by incubation in 3% bovine serum albumin (with 0.5% Triton X-100 dissolved in 0.1 M PB, Sigma) for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... media were aspirated and cells viability was determined by addition of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma) at a final concentration of 0.5 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... The pellet was resuspended and incubated for 30 min at 4 °C in buffer B (10 mM Tris pH 7.5, 2 mM MgCl2, 3 mM CaCl2, 0.5% IGEPAL CA-630, 10% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Molecular Biology 2022Quote: Cell growth was monitored by cell counting and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay using MTT cell growth assay kit (#CT02, Millipore). FL or ΔN-Drosha cells (1×105 ...
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bioRxiv - Bioengineering 2022Quote: For HP-β-CD-treatment 3×SNCA hMOs were exposed to 5 μM HP-β-CD (Sigma Aldrich) in cell culture media starting from D10 of differentiation.
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Biochemistry 2022Quote: ... D-glucose N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich, USA ...
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bioRxiv - Cancer Biology 2022Quote: ... following additional 5 min incubation with 100 nM Anionic Oxonol Dye DiBAC4(3) (D8189, Sigma Aldrich, MO, USA). Fluorescence was analysed on a BD FACSCalibur flow cytometer ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were incubated with washing buffer (PBS/0.05% Tween) and blocked with PBS/0.05%Tween/3%BSA (Sigma). Subsequently the wells were washed and the primary anti-Cas9 antibody (Cas9-7A9-3A3 ...
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bioRxiv - Cancer Biology 2022Quote: ... Transfected cells were grown for 3 days prior to adding hygromycin B (400μg/mL; Millipore Sigma, Catalog #400050) or puromycin (1µg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Aliquots were taken at different time points and kept on ice with 3 units of nuclease-P1 (Sigma). One hour after nuclease-P1 incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... 400μL were mixed with 200μL of a 3 mg/mL collagen in 0.1% acetic acid solution (rat tail type I; Merck Millipore), followed by the addition of 10μL of 1 M NaOH ...
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bioRxiv - Cell Biology 2022Quote: ... was washed with IP-lysis buffer and incubated with 3 µg of mouse anti-Flag antibody (Sigma, M2) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Ten ml of each mix was placed on a silanized with 0.5% 3-aminopropyltrimethoxy-silane (ACROS) and activated with 0.5% glutaraldehyde (Sigma) glass-15mm coverslip and covered with a 13mm coverslip pre-coated with fibronectin (1mg/ml) ...
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bioRxiv - Cell Biology 2022Quote: Cultures were fixed in 4% paraformaldehyde and stained with antibodies against α-tubulin (Millipore MAB1864; 3 μg/mL), MUC5AC (ThermoScientific 45M1 ...
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bioRxiv - Immunology 2022Quote: ... To asses mitochondrial fuel usage OCR was measured subsequent to the addition of the following drugs in different combinations as indicated: 3 μM bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (SML0601, Sigma), 2 μM UK-5099 (PZ0160 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5 μM RA treatments on days 0 and 4 plus 10 nM BIO (6-Bromoindirubin-3’-oxime, Sigma) treatment on day 0 ...
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bioRxiv - Immunology 2022Quote: ... the bacterial pellet was re-dispersed in PBS supplemented with 10 % 1M NaHCO 3 (Cat#S5761, Sigma-Aldrich) with the final OD 600 of 5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Roots were then squashed under coverslips onto slides that had been pre-treated with 3-Aminopropylthiethoxysilane (Sigma-Aldrich) to increase tissue adherence (4) ...
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bioRxiv - Microbiology 2022Quote: ... They were then subjected to bead beating (3 × 45 s) with glass beads (particle size ≤ 106 μm; Sigma) using a MP Biomedicals™ FastPrep-24™ (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peroxidase activity was revealed by incubating slides in 3-amino-9-ethylcarbazole (AEC) (Sigma, St Louis, MO, USA) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... N-(3-aminopropyl)methacrylamide hydrochloride (APMA) and N,N′-bis(acryloyl)cystamine (BACA) were purchased from Sigma-Aldrich. Peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS and 10% 5mg/mL 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent (SIGMA) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... we delivered the Iκ-kinase inhibitor [5-(p-Fluorophenyl)-2-ureido]thiophene-3-carboxamide (TPCA-1; Sigma-Aldrich CAS 507475-17-4 ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were enzymatically dissociated for 20 min at 37°C with papain (3 U/mL, Sigma-Aldrich), diluted in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Physiology 2023Quote: ... Samples were separated on a SeQuant ZIC-pHILIC column (100 3 2.1 mm, 5 mm, polymer, Merck-Millipore) including a ZIC-pHILIC guard column (2.1 mm x 20 mm ...
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bioRxiv - Microbiology 2022Quote: ... female 3–4-week-old BALB/c or C57BL/6 mice were injected intraperitoneally with 10mg azoxymethane (Sigma) per kg mouse weight ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptors were blocked using 6,7-dinitroquinoxaline-2,3-dione (DNQX, Sigma) dissolved in dimethyl sulfoxide and diluted by 1000 in aCSF for 10μM bath application.
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bioRxiv - Neuroscience 2022Quote: ... Slides were blocked by incubation in 3% bovine serum albumin (with 0.5% Triton X-100 dissolved in 0.1 M PB, Sigma) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... or MG132 using a MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-based in vitro toxicology kit (TOX1-1KT, Sigma). 10 µl MTT solution was applied to cells at the end point of the experiment and incubated for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001 ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was concentrated to ∼500 μL using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore MWCO 3 kDa). The concentration of proteins was determined using JASCO V730 UV-vis spectrophotometer at 280 nm ...
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bioRxiv - Plant Biology 2022Quote: ... the same total protein extracts were incubated with Endoglycosidase H at 37 °C for 3 h (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... or 10nM GIP in stimulation buffer (1x Hanks Buffered Saline Solution, 0.1% BSA, 0.1% 3-isobutyl-1-methylxanthine (IBMX) (all Sigma), 0.5mM HEPES (Invitrogen) ...