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Citations for Millipore Sigma :
951 - 1000 of 10000+ citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed in PBS-T for 40 min and the bound proteins were visualized with anti-FLAG-HRP antibodies (1:10000 in PBS-T for 1h, Sigma-Aldrich, Cat. no: A8592). All steps were performed at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Flat-mounted retinas were incubated overnight at 4°C with antibodies against RNA-binding protein with multiple splicing (RBPMS; EMD Millipore Corp., Burlington, MA) diluted 1:500 in blocking buffer (2% bovine serum albumin ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 3 hours with primary antibodies directed to either AGO proteins (WAKO anti-AGO2 #011-22033, EMD Millipore anti-panAGO #MABE56) or directed to T6B-fusion protein (Cell Signaling anti-FLAG #8146S ...
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bioRxiv - Neuroscience 2020Quote: ... anti-amyloid precursor protein (APP; mouse anti-human monoclonal antibody clone 22c11, dilution 1:10, epitope retrieval time 10 min, MAB348; EMD Millipore, Burlington, MA, USA); anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2022Quote: ... Labelled RNAs in the eluate were then detected by autoradiography and proteins were subjected to WB using an anti-Flag antibody (Sigma-Aldrich F3165; 1:10000).
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bioRxiv - Molecular Biology 2022Quote: ... and expression levels of AGAL-FLAG tagged proteins were assessed by Western blot with mouse monoclonal anti-FLAG antibody (F1804, SIGMA-Aldrich, Prague, Czech Republic). Clones demonstrating similar GLA_FLAG transcript and AGAL-FLAG protein amounts were selected for further analyses.
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bioRxiv - Molecular Biology 2023Quote: ... Histidine tagged PDE4 and GST tagged CP proteins were detected chromogenically using anti-mouse secondary antibodies labelled with alkaline phosphatase (1:30000) (cat#A3562, Millipore Sigma, Burlington, MA, USA) and western blue stabilized substrate for alkaline phosphatase (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Cells were then fixed using 10% formalin and processed for immunofluorescence using an antibody against the HCoV-OC43 N protein (Millipore #MAB9013; diluted 1:500) and a secondary Alexa Fluor 555 conjugated anti-mouse IgG antibody (NEB #4413S ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were normalized to either actin or GAPDH housekeeping proteins with monoclonal antibodies (anti-actin; 1∶1000, Sigma or anti-GAPDH; 1:10,000 Abcam).
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). Following five 5-minute washes at 20°C with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and a primary rat antibody against myelin basic protein (MBP) (1:50 in blocking solution; Cat. No. MAB386, Sigma-Aldrich, St. Louis, MO, USA). The following day ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Bradford protein assay was used to quantify total protein levels (Bradford, Sigma-Aldrich, USA) and to normalize BDNF data.
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total carbonylated proteins were quantified using OxyBlot Protein Oxidation Detection Kit (Millipore, Billerica, MA) following manufacturer’s instructions using 15 μg of total protein detected with rabbit anti-DNP antibodies (Invitrogen ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Bound proteins were captured with Protein G agarose for 4 h (Sigma-Aldrich, #17061801), spun at low speed ...
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bioRxiv - Cell Biology 2023Quote: ... and total (i.e. nonphosphorylated) protein levels were normalized to tubulin protein levels (Sigma-Aldrich, #T6557 ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 μg of protein were added to washed agarose protein A beads (Merck Millipore) and incubated on a rotating wheel for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and visualized using InstantBlue Coomassie Protein Stain or ReadyBlue Protein Gel Stain (Sigma Aldrich).
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bioRxiv - Molecular Biology 2024Quote: ... Protein amounts were measured using the bicinchoninic acid assay (BCA Protein Assay Kit, Novagen) on a NanoDrop ND-1000 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2024Quote: ... The resulting protein mixtures were filtered using a low protein binding filter (Millipore Sigma), and purified through a Source 15Q anion exchange column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were either stained for protein content using ReadyBlue Protein Gel Stain (Sigma-Aldrich) or subjected to in-gel peroxidase activity assays following a modified ferricyanide protocol.80 In-gel detection of peroxidase activity was initiated by incubating gels with a 0.05% 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2021Quote: ... protein standards (Merck Sigma-Aldrich) specified in the Supplementary Material were separated on the same column.
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bioRxiv - Biochemistry 2020Quote: ... Protein A/G beads (Millipore) were then added for overnight incubation at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein G affinity beads (Sigma) were added to the lysate (for anti-D-cys immunoprecipitation only ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0 Protein Detection System (Millipore). Membranes were imaged using a chemiluminescent SuperSignal West Femto Maximum Sensitivity Substrate (34095 ...
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bioRxiv - Cancer Biology 2021Quote: Protein A Dyna-beads (Millipore) were used for immunoprecipitation ...
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bioRxiv - Physiology 2022Quote: ... Protein G Agarose beads (Millipore) and AMPK-IP buffer (50 mmol l-1 NaCl ...
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bioRxiv - Biophysics 2022Quote: ... 1x Protein Inhibitor Cocktail (Sigma), 1 mM PMSF (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: BCA protein assay (Sigma-Aldrich) was performed to measure protein concentrations ...
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bioRxiv - Developmental Biology 2022Quote: ... 5 mM Cas9 protein (Sigma), and 300 mM KCl ...
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bioRxiv - Cancer Biology 2020Quote: ... protein G agarose (60µL, Millipore) was incubated with anti-phosphotyrosine antibodies (12µg 4G10 (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A Sepharose beads (Sigma) were then added and the samples were incubated at 4°C for another hour with rotation ...
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bioRxiv - Plant Biology 2021Quote: ... Protein A-agarose beads (Millipore) and an antibody against H3K9ac (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... or S-protein beads (Millipore) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G sepharose beads (Sigma) were then placed in the mixture for 4 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G agarose beads (Sigma) were activated by immersing in water and incubated in IP buffer (300 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Protein G Magnetic beads (Millipore) were used for pull-down of PPARG-chromatin complex and analysed using PCR after reversal of cross-linking.
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bioRxiv - Immunology 2022Quote: ... and either Ovalbumin protein (Sigma) or mMOGtag protein.
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Myelin Basic Protein (MBP; Millipore) was used as a positive control ...
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bioRxiv - Neuroscience 2022Quote: ... 2.0 Protein Detection System (Millipore). The following antibodies were used ...
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bioRxiv - Physiology 2022Quote: Magnetic protein G beads (Sigma) were used per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... Protein Detection System (EMD Millipore), according to the manufacturer’s instructions ...