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Citations for Millipore Sigma :
7551 - 7600 of 10000+ citations for Anti IL 4I1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... FLAG- TOP3A-RMI1/2 or FLAG-DNA2 were labeled with QDs pre-incubated with a mouse anti-FLAG antibody (Sigma-Aldrich) on ice for 10 minutes prior to injection ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... the cells were incubated for 1 hour at 37°C with 1:300 mouse anti-α-tubulin antibody (Sigma Aldrich, T6199) in PBS + 1 % BSA ...
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bioRxiv - Biophysics 2022Quote: ... cells were permeabilized with 0.3% Triton X-100 in PBS and then incubated with anti-α-actinin mouse monoclonal antibody (1:100, A7732, Sigma-Aldrich) in CAS-Block reagent (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: GMPCPP-stabilized microtubule seeds polymerized from biotinylated and TAMRA-labeled bovine tubulin were attached to the surface of a microchannel using anti-biotin antibodies (Sigma-Aldrich). Microtubules were assembled using 12 μM unlabeled bovine GTP-tubulin for 10 min at 28 °C in polymerization buffer (80 mM K-PIPES ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then incubated with primary antibodies overnight at 4°C: anti-Alpha Tubulin (1:200, DSHB 12G10) and β-catenin (1:10,000 dilution, Sigma ABE208). The membrane was washed three times using PBST ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates collected were run in SDS-PAGE (30 μl loaded) and blotted with the following primary antibodies: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... and then immunoblotted with the following primary antibodies in TBST with 5% milk at 4°C overnight: mouse anti-Miro1 (WH0055288M1, Sigma-Aldrich) at 1:1,000 ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... α-PilC 20 and α-RomY antibodies were used together with goat anti-rabbit immunoglobulin G conjugated with horseradish peroxidase (Sigma) as secondary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... or a concentration of 50 nM for IMAC-purified DARPins) binding to CagI was performed using a mouse-anti-FLAG M2 monoclonal antibody (dilution 1:5000; Sigma, F1804) as primary and a goat-anti-mouse antibody conjugated to an alkaline phosphatase (dilution 1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... was loaded alongside the pulldown samples which were resolved on 10% SDS polyacrylamide gels and subject to western blotting using anti-GST antibody (Sigma G7781). Membranes were developed with an ECL plus Chemiluminescent kit (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... 15 cycles of 30 s on and 30 s off) and immunoprecipitated using an anti-GFP antibody (Sigma-Aldrich; Merck KGaA). The immunoprecipitated and purified DNA was used for quantitative real-time PCR ...
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bioRxiv - Neuroscience 2022Quote: ... brains were first cut into 50 um slices and store at 4C in .1% sodium azide PBS before undergoing NeuN staining procedures using Alexa Fluor 488 Conjugate ABN78A4 Anti-NeuN (rabbit) antibody (Sigma-Aldrich). Slices were washed 3 times for 10 minutes with 1x PBS and pre-incubated in 10% Horse Serum and 0.3% Triton-X-100-PBS with 1% BSA for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... Total NUC/GEN polypeptide abundance was detected by probing duplicate blots with a mouse anti-GFP antibody (#11814460001; Sigma Aldrich (Roche), Darmstadt ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were washed as before and incubated with an HRP-conjugated anti-FLAG antibody (clone M2, Sigma-Aldrich, 1:10.000 dilution) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... was used as a substrate for in situ hybridization and immunolabeling was carried out with primary antibodies anti-GABA (1:500, Sigma–Aldrich), anti-GFP (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... The membranes were subjected to the following primary antibodies overnight at 4°C according to the experiment: mouse monoclonal anti-β-tubulin III (1:5000; Sigma) or mouse monoclonal anti-actin (1:4000 ...
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bioRxiv - Molecular Biology 2022Quote: ... The membrane was washed and subjected to Anti-DIG-AP antibody by using DIG Wash and Block Buffer Set (Sigma, #11585762001) and immonological detection of the membrane was conducted by CDP-Star chemiluminescent substrate included in the northern blotting kit.
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Biochemistry 2022Quote: ... 2.5 μL time points were taken and quenched with 2 mM EDTA then brought to 20 μL final volume for detection using 1:500 dilution anti-H3K4me3 antibody (05-745R, EMD Millipore), 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082) ...
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Neuroscience 2022Quote: ... the cells were incubated with the corresponding primary antibodies diluted in blocking solution: anti-tyrosine hydroxylase (TH) (Millipore, #MAB318; 1:2000) or anti-NeuN (Millipore ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Antibodies used for immunoblotting in this study include rabbit polyclonal anti-GFP (ab290, Abcam, 1:2,500 or Sigma, G1544, 1:1000), mouse monoclonal anti-GFP (JL8 ...
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bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: ... and incubated overnight in the same solution at 4°C with a 6000 X dilution of anti-HA-peroxidase antibodies (Sigma-Aldrich) in 0.5 % milk in TBS-Tween 0.1 % to reveal HA-tagged NIN proteins following chemiluminescence revelation using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... For staining the following primary antibodies were used: For labeling of nerve fibers mouse anti-beta-Tubulin III (1:200, Sigma, T8578); for pre-synaptic terminal staining rabbit anti-Synapsin ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 and incubated overnight at 4°C followed by an secondary anti-rabbit HRP conjugated antibody (12-348, Merck Millipore, Germany) diluted 1:5000 in PBST and incubated for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated in blocking solution (5% normal donkey serum and 0.3% Triton X-100 in PBS) for 30 minutes and then incubated in rabbit anti-TH polyclonal antibody (Millipore, Cat: AB152) diluted 1:1000 in 0.3% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... for 1 h and incubated overnight at 4°C with the specific primary antibody: mouse anti-NeuN 1:200 (Millipore, MAB377), rabbit anti-GFAP 1:1000 (Dako ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.25% Triton X-100 for 30 min at RT and subsequently incubated in 0.1% Triton X-100 and 1% NS in PB with the following primary antibodies: mouse monoclonal anti-NeuN (1:1000; Millipore, #MAB377), anti-SMI32 (specific for non-phosphorylated neurofilament H ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 and 10% foetal calf serum in PBS] containing primary antibodies including: mouse anti-NeuN (1:200; Millipore, #MAB337); mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... iBECs were cultured in Transwell inserts with 3.0 µm pores and exposed to FITC-conjugated 150 kDa dextran (0.5 mg/ml) or anti-TDP-43 antibody (1 µM, Sigma, Cat#T1705) and 10 µl of phospholipid-shelled microbubbles with octafluoropropane gas core prepared in-house as described in (40) ...
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bioRxiv - Neuroscience 2024Quote: ... for one hour before overnight incubation at 4℃ with one of the following primary antibodies: anti-NG2 (1:200; Millipore AB5320), anti-Laminin (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed five times with five-minute incubations in PBST and then incubated overnight in primary antibody solution containing anti-acetylated alpha-tubulin (1:500; Sigma, T6793) in PBST plus 20% NGS ...
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bioRxiv - Developmental Biology 2024Quote: ... They were blocked in 1% BSA in PBT for 1hr at RT and incubated overnight at 4°C with the monoclonal mouse anti-FLAG M2 antibody (Sigma, F1804) diluted 1:500 in 1% BSA/PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP ...
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bioRxiv - Immunology 2024Quote: ... Viral nucleoprotein (NP) was detected by ELISA as described using a 1/1,000 dilution of the anti-NP antibody (Millipore cat. #MAB8257) and a 1/2,000 dilution of the horseradish peroxidase-labeled goat anti-mouse IgG (SeraCare cat ...
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bioRxiv - Microbiology 2024Quote: ... and treated with 16 μM Hoechst 33342 and/or Anti-Human IgG (Fc specific)−FITC antibody (1:100 dilution, Sigma-Aldrich) in iCM ...
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bioRxiv - Microbiology 2024Quote: ... correct production of the protein of interest was checked by Western Blot of HEK293T-transfected cells using an anti-Flag antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... Filters were scanned by densitometry and the relative phosphorylation level of each MAPK member was determined relative to anti α-tubulin antibody T9026 (Sigma) loading control ...
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Genetics 2023Quote: ... were washed with 1% BSA in PBS and pre-loaded with Anti-RNA polymerase II antibody (Millipore,05-623, Lot 3506610). Antibody-conjugated beads were washed twice with 1% BSA and incubated with sheared chromatin in dilution buffer (1% Triton X -100 ...