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Citations for Millipore Sigma :
6951 - 7000 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Tryptophan specialized metabolism and ER body-resident myrosinases modulate root microbiota assemblybioRxiv - Plant Biology 2022Quote: ... ethanol and bleach with 12% (w/v) active chlorine and germinated on metal meshes placed on half-strength Murashige and Skoog (MS) basal salts (Sigma-Aldrich), supplemented with 1% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... we provided a buprenorphine solution (0.1 g/L, Temgesic®) mixed in drinking water containing 5% (w/v) D-Glucose (G8769-100ml, Sigma Aldrich) to mask the drug taste ...
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bioRxiv - Microbiology 2024Quote: ... cells were diluted (1:10000) into new microtiter plates with fresh TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK). The plates were sealed with a gas permeable membrane (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa strains with the arabinose-inducible vector and its derivatives were grown in LB with 133 mM L-arabinose (2% w/v) and 300 μg/ml carbenicillin (both from Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... and collected on wet ice in 4°C RIPA lysis buffer (150 mM Tris-HCl, 150 mM NaCl, 0.5% (w/v) sodium deoxycholate (Sigma-Aldrich, D6750), 1% (v/v ...
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bioRxiv - Neuroscience 2024Quote: Oligomycin and antimycin A (inhibitor of mitochondrial complex V and III respectively) were purchased from Sigma-Aldrich (O4876 and A8674 respectively).
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bioRxiv - Bioengineering 2024Quote: TEM grids functionalized with Strep-AuNPs were blocked with a 3 % (w/v) of bovine serum albumin (BSA) solution (Sigma-Aldrich) diluted in PBS for 15 min to reduce non-specific capture ...
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bioRxiv - Bioengineering 2024Quote: ... very low- and low-density lipoprotein [(V)LDL] were separated from the plasma above using a HDL and LDL/VLDL Quantitation Kit (Sigma-Aldrich) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 500 µl of the sample was mixed with 750 µl of 50%(w/v) iodixanol (a mixture of 4 ml of OptiPrepTM gradient (Sigma-Aldrich) and 0.8 ml of diluent [150 mM KCl ...
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bioRxiv - Biochemistry 2024Quote: ... the induction was done either by 1% (w/v) lactose or 0.5 mM isopropyl-β-D-1-thio-galactopyranoside (IPTG, Sigma Aldrich) (Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Percentage survival was determined by manual cell counting of at least 50 cells or more66 after staining with crystal violet staining solution (0.05% w/v crystal violet, 1% formaldehyde, 1% MeOH, in 1x PBS) (Sigma-Aldrich C0775).
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bioRxiv - Neuroscience 2024Quote: Mice aged 3 to 5 months were deeply anesthetized and transcardially perfused with 50mL PBS followed by 50mL 4% (w/v) paraformaldehyde (Sigma-Aldrich) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Single-cell suspensions were obtained after mechanical trituration (10 times with both a p1000 and a p200 pipet) in sterile PBS with 1% bovine serum albumin (fraction V, Sigma-Aldrich). To ensure singlets ...
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bioRxiv - Microbiology 2024Quote: ... Standard protocol was used for DNA extraction with the CTAB lysis buffer containing 2% w/v CTAB (Sigma-Aldrich, Poole, UK), 100 mM Tris–HCl (pH = 8.0 ...
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bioRxiv - Synthetic Biology 2023Quote: ... in which strains were grown in Synthetic Complete (SC) media with 2% (w/v) glucose using appropriate amino acid dropout mixes (Sigma-Aldrich) and/or with the addition of antibiotic nourseothricin (100 mg/L ...
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bioRxiv - Biophysics 2023Quote: ... The remaining solution was removed from the wells and the cells were washed once with 1XTBS followed by incubation with 200μL of 0.2% (w/v) Janus green B (Sigma, Cat. no. 201677), a mitochondrial stain ...
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bioRxiv - Microbiology 2023Quote: ... The integrity of the BSA was assessed after separation by 10% (w/v) SDS-PAGE and staining using Coomassie blue (ISB1L – Sigma-Aldrich). The resultant gel was imaged using an Azure 600 scanner (Azure Biosystems).
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bioRxiv - Neuroscience 2023Quote: ... Either TA or soleus muscles were surgically exposed and injected with 30-40 μl of 2% (w/v) Evans blue dye solution (Sigma Aldrich) in phosphate buffered saline (PBS ...
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bioRxiv - Systems Biology 2022Quote: ... For urine samples a 1:5 (v/v) dilution was prepared in water prior to a urease digestion at 37°C for 40min with 10U urease (Sigma Aldrich). For each sample ...
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bioRxiv - Cell Biology 2023Quote: ... Rinsed membranes were incubated in primary antibody solution (primary antibody reserved in 5% bovine serum albumin, BSA, Sigma, 0.1% (w/v) NaN3 in TBS ...
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bioRxiv - Immunology 2023Quote: ... Liquid cultures were prepared from bacterial plates before washing in PBS and diluting in 2% (w/v) polyvinylpyrrolidone40 (PVP40, Sigma-Aldrich) for injection as described previously (Benard et al ...
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bioRxiv - Bioengineering 2023Quote: NaH2PO4-crosslinked SF tubular scaffolds (150 layers, 60° winding angle) were immersed in a 1% w/v solution of phosphotungstic acid (Sigma Aldrich) as contrast agent for 3 h ...
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bioRxiv - Neuroscience 2023Quote: ... a retro-orbital injection was performed to deliver 100 µL of solution containing 20 µL of Pluronic F-127 (20% w/v; Sigma Aldrich), 20 µL of DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... and homogenised in 10% (w/v) sterile phosphate-buffered saline (PBS) (containing protease inhibitor cocktail, phosphatase inhibitor cocktails I and II (Sigma, UK), at a final dilution of 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by second round of concentration by loading on an Iodixanl-density gradient media (OptiPrep 60% w/v D1556 – Sigma, below), and centrifuge in a Beckman WX Ultra90 centrifuge at 41,000rpm and 180C for 2.5h ...
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bioRxiv - Microbiology 2023Quote: ... 3.9 mM KH2PO4, 9.3 mM NH4Cl, 0.5 mM MgSO4, 0.5 mM CaCl2, 0.01 mM FeSO4 [EDTA-chelated; Sigma, F0518], 0.2% [w/v] glucose) supplemented with isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Cell Biology 2023Quote: ... zona-less E2.5 embryos were immobilised on gelatine-coated microscope slides by fixation in 1% (w/v) paraformaldehyde (Sigma-Aldrich, #P6148) in PBS ...
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bioRxiv - Cell Biology 2023Quote: Left hind limbs of mice were collected and fixed overnight at room temperature (with agitation) in 10% (w/v) neutral-buffered formalin (Sigma-Aldrich). Limbs were washed with distilled water before decalcification with Formical-2000 (American MasterTech Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µg of cell extract was loaded into SDS PAGE gel (15% w/v) and transferred to PVDF membrane (Millipore Corp.). After transferring proteins to PVDF ...
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bioRxiv - Biophysics 2023Quote: ... The remaining exposed surface was blocked for 5 minutes with 6% Pluronic F-127 (w/v) (Sigma P2443. MW: 12.5 kDa) and 15mg/ml of Bovine Serum Albumin (BSA ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was done for 30 minutes at room temperature in AbDil or TBS-T + 2 w/v% Bovine serum albumin (BSA, Sigma Aldrich) for 2D and in TBS-T + 5 w/v% BSA for 3D ...
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bioRxiv - Genomics 2023Quote: ... The undigested tissue was retrieved from the filter by forceps and placed in a 50 mL Falcon tube containing 12.5mL of prewarmed 1mg/ml Collagenase V (Sigma-Aldrich, C9263) and 40µg/mL DNase I (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... MAs were liberated from the dried cell debris by heating at 90 °C in 40% (w/v) tetrabutylammonium hydroxide (TBAH; Sigma-Aldrich) for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... the wells were washed and 25 μL of 1 mM ifosfamide solution in AIM-V were added with or without ritonavir (1 μM, Sigma-Aldrich). 5μL supernatant solutions were collected after 0 ...
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bioRxiv - Microbiology 2023Quote: ... cells were split into two and each resuspended in 250 μL PBS containing 1% w/v bovine serum albumin (BSA, Sigma, 10735078001) with or without the addition of 0.1% w/v saponin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... The glass nanopore was then immersed in a similar buffer with the addition of 50% (w/v) Polyethylene Glycol (PEG) 35 kDa (ultrapure grade, Sigma Aldrich). The notation of the 50% PEG in the text refers to the utilization of 50% (w/v ...
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bioRxiv - Plant Biology 2023Quote: ... and supernatant was mixed with 6 μL loading buffer (750 mM ε-aminocaproic acid and 5% (w/v) pure Coomassie Brilliant Blue G (B0770, Sigma)) and loaded onto a 6-12% gradient polyacrylamide gel ...
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bioRxiv - Plant Biology 2023Quote: ... stratified and stored at 4°C before sowing on sterile room temperature rectangular plates prepared in sterile conditions with solid media consisting of 0.175% w/v Murashige and Skoog Basal Medium (MS) (Sigma-Aldrich, UK), 0.5% w/v sucrose (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Free-hand root cross-sections obtained with sharp razor blades were placed into distilled water and subsequently stained with fresh fluorol yellow 088 (0.01% w/v, in methanol) (Sigma-Aldrich, Merck) at room temperature for 1 h in darkness and rinsed and kept into distilled water before observation ...
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bioRxiv - Plant Biology 2023Quote: ... vapor- sterilized seeds were sown on 1X MS medium (Murashige and Skoog, 1962) containing 0.8% Phytagel [w/v] (Sigma-Aldrich, P8169) and stratified at 4°C for 2-3 days ...
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bioRxiv - Synthetic Biology 2024Quote: ... The nanopipette was then immersed in a 100 mM KCl bath with the addition of 50% (w/v) polyethylene glycol (PEG) 35 kDa (ultrapure grade, Sigma Aldrich). A working electrode consisting of Ag/AgCl wire (0.25 mm diameter ...
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bioRxiv - Neuroscience 2023Quote: ... demyelination was induced in the ventral white matter funiculus of the thoracic spinal cord (between vertebrae T11-12 or T12-13) by the injection of 1.2 µL of 1% (w/v) L-α-Lysophosphatidylcholine (Lysolecithin; Sigma-Aldrich) under general anaesthesia ...
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bioRxiv - Bioengineering 2023Quote: ... % Plu and PluDA stock solutions (named DA0 and DA100, respectively) were prepared in milliQ water and contained 0.2% w/v Irgacure 2959 (Sigma-Aldrich Co.) as photoinitiator ...
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bioRxiv - Bioengineering 2023Quote: ... The high-swelling layer of the scaffold was made with a 15% w/v gelatin in phosphate-buffered saline solution (PBS, Sigma-Aldrich) 1X crosslinked with 92 μl/g of GPTMS (GPTMS-GEL-15) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of the cell suspension was mixed with an equal volume of 0.4% w/v trypan blue solution (T8154-20ML, Sigma-Aldrich, USA), and the cell viability (percentage of unstained cells ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were stained in 150 mM phosphate buffer pH 8.0 containing 0.01% (w/v) aniline blue (Sigma Aldrich- 28631-66-5) at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then incubated for 30 min at 37°C in blocking buffer [0.5% (w/v) bovine serum albumin (BSA, Sigma Cat. #A9647), 10% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... respectively) by dilution in a vehicle solution of 10% w/v 2-hydroxypropyl-!3-cyclodextrin (Sigma-Aldrich, St-Louis, MO, USA) in PBS ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...