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Citations for Millipore Sigma :
7101 - 7150 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50μg of lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Microbiology 2021Quote: ... USA) QIAamp 96 DNA QIAcube HT Kit with the following modifications: enzymatic digestion with 50 μg lysozyme (Sigma, St. Louis, MO, USA) and 5U each of lysostaphin and mutanolysin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ShScrambled/α-cyclo and shAbcd/C26:0 neurons were treated every 24 h for 96 h with 5 μM of the selective GSK inhibitor SB216763 (Sigma Aldrich; S3442) dissolved in dimethyl sulfoxide (DMSO) ...
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bioRxiv - Cancer Biology 2021Quote: ... Normal pancreata from the tdTomato progeny and from EGLN1/2/3fl/fl mice and whole tumors from KPC mice were minced and digested with 1 mg/mL Collagenase V (Sigma, C9263-500MG) for 30 minutes at 37°C and 130 rpm/min followed by digestion with TrypLE (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were blocked in a blocking solution including 1% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) in PBS ...
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bioRxiv - Developmental Biology 2021Quote: Live zebrafish embryos and larvae were first anesthetized by bath application of 0.02% w/v solution of 1025 Ethyl-3-aminobenzoate methanesulfonate (Sigma-Aldrich, St. Louis) in filtered fish system water for 1 min ...
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bioRxiv - Cell Biology 2019Quote: ... The desired Young’s modulus (E) of the pre-mixes was adjusted by mixing pre-defined ratios of 40% (w/v) acrylamide monomer (Sigma-Aldrich, A4058) and 2% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 titres were enumerated using plaque assays on Vero E6 cells as previously described [133] using equal parts 2.4% w/v semi-solid colloidal cellulose overlay (Sigma; prepared in ddH2O) and 2X DMEM (Wisent ...
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bioRxiv - Neuroscience 2021Quote: ... the mouse was perfused at 0.6 ml/min with 5 ml of a 0.1% (w/v) fluorescein isothiocyanate (FITC) conjugated albumin (Sigma-Aldrich, cat.no.: A9771-1G) in a 2% (w/v ...
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bioRxiv - Immunology 2019Quote: ... erythrocytes were depleted by sequentially layering 10 ml PBS followed by 8 ml of 6% w/v dextran (high molecular weight, Sigma-Aldrich, in PBS) and gently inverting ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were then diluted in synthetic complete low-fluorescence media (SC-LFM) and either 2% galactose (Acros, cat. 150615000) or 2% (w/v) dextrose (Sigma, cat. G5767). After three more hours of incubation ...
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bioRxiv - Microbiology 2019Quote: ... For this purpose, proteins were blotted semidry (12 V, 20 min) on a PVDF membrane (PSQ membrane, 0.2 µm, Merck Millipore, Burlington, Massachusetts, USA). After incubating the membrane 5 min in 3 % TCA ...
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bioRxiv - Molecular Biology 2021Quote: ... Probes were denatured for 5 min at 95 °C before their addition to the hybridization solution (10% w/v dextran sulfate, Sigma D-6001), 10% v/v deionized formamide (VWR 97062-008) ...
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bioRxiv - Bioengineering 2021Quote: ... The gel compartment was removed after 1 to 5 days of incubation (depending on fusion) by adding 4 w/v% sodium citrate (Sigma Aldrich Inc.) for 15 min as a lyase for alginate (Supplementary figure 5 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then permeabilized for 30 minutes at room temperature in a 0.05% w/v saponin solution dissolved in PBS (Sigma, quillaja bark powder), and then washed 3X with PBS before being incubated with the staining reagents.
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bioRxiv - Cell Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with crystal violet (1% w/v in 20% methanol, Sigma, cat# C0775) for 1 hour ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were surface-sterilized and imbibed for 4 days at 4°C in the dark on 0.7% (w/v) agar plates containing half-strength Murashige and Skoog (MS) media (Sigma-Aldrich; pH 5.7). Seeds were subsequently germinated and grown at 22°C under short-day conditions (8 h light/16 h dark) ...
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bioRxiv - Microbiology 2021Quote: ... we transferred 1/100 volume of overnight culture to fresh LB-Amp media that additionally contained 1% L-arabinose (w/v) (Sigma-Aldrich #A3256) to induce the expression of Lambda Red recombinase ...
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bioRxiv - Plant Biology 2021Quote: ... Plants were grown either on ½ Murashige and Skoog medium (MS) medium (Duchefa Biochemie, Netherlands) with 1% sucrose and 0.6% [w/v] phytagel (Sigma-Aldrich, Inc., Germany) or on standard plant potting soil pretreated with Confidor WG 70 (Bayer Agrar ...
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bioRxiv - Microbiology 2022Quote: ... and blocked for 3 h at 4°C in 60 ml blocking buffer composed of TBST buffer supplemented with 5% (w/v) BSA (Sigma Life Science). During the blocking stage ...
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bioRxiv - Cell Biology 2022Quote: ... The different parasite stages were detected through double-labelling with stage-specific marker primary antibodies or 0.001% w/v Evans blue (Sigma Aldrich, Taufkirchen, Germany) followed by incubation with Alexa Fluor 594-conjugated secondary antibodies (Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl of the sample was mixed with 750 μl of 50%(w/v) iodixanol (made by mixing 4 ml of OptiPrep™ gradient (Sigma-Aldrich) and 0.8 ml of diluent [150 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V using 1 X NuPAGE Running Buffer [50 mL of 20 × 1 L Stock [209.2 g MOPS (Sigma-Aldrich, M1254-1KG), 121.1 g Bis-Tris (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 8% bleach and sterile water, were sown on 1/2 Murashige and Skoog (MS) salts agar (1%, w/v) plates (Sigma-Aldrich, CAD) with 1% sucrose ...
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bioRxiv - Biochemistry 2021Quote: ... and 0.5% w/v of dextran sulfate sodium salt from Leuconostoc spp. with sizes ranging from 6500 to 10000, Sigma-Aldrich in PBS). The substrate was aliquoted and stored at – 80 °C until required ...
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bioRxiv - Biophysics 2019Quote: For transcription reactions σ70RNAP549 holoenzyme was introduced into the chamber at 1 nM in transcription buffer (wash buffer supplemented with 3.5% w/v PEG 8,000 (#81268; Sigma-Aldrich; St. Louis, MO), 1 mg mL-1 BSA ...
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bioRxiv - Cell Biology 2019Quote: ... samples were fixed in 4% paraformaldehyde (w/v) in 0.05M phosphate buffer pH 7.3 for 24 hours and embedded in Paraplast (Sigma Aldrich, São Paulo, Brazil). Sections of 5 μm thickness were placed on silanized slides ...
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bioRxiv - Genomics 2020Quote: ... and tissue was enzymatically digested in a solution (1X HBSS, 20mM HEPES, 30mM sodium bicarbonate, 1% BSA fraction V) containing collagenase type IV (200 U/mL; Sigma C-5138); hyaluronidase Type IS (1mg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... HMPs were incubated with either a single or dual growth factor solution (Figure 1C: G1, G2) in 1 mL of 0.1% (w/v) bovine serum albumin (BSA; Millipore Corporation, Billerica, MA) in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were assayed with a 2-min mix/2-min wait/5-min measurement cycle for three baseline measurements followed by three cycles after each injection of four compounds affecting bioenergetics: 0.5μM oligomycin (Complex V inhibitor; Sigma, St. Louis, MO, USA), 0.7μM carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP ...
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bioRxiv - Neuroscience 2021Quote: ... adding a drop of water to prevent the gel from drying (ibidi chambers and coverslips were poly-lysine coated by incubation with poly-l-lysine solution (0.1% w/v in water (P8920, Sigma-Aldrich, lot: 050M4339) for at least 45 min at RT shaking and dried with pressured air) ...
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bioRxiv - Genetics 2019Quote: ... transferred to nitrocellulose membranes and probed with the following primary antibodies diluted in 5% (w/v) nonfat-milk in TBS buffer + 0.1% Tween 20: α-Flag (Sigma-Aldrich, F3165), α-ORC2 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were grown in T75 flasks in a humidified atmosphere of 5% CO2 in air at 37°C and removed from the flask surface using incubation with trypsin (0.05% w/v) (Sigma Aldrich, Poole, UK) containing EDTA (0.03% w/v ...
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bioRxiv - Microbiology 2021Quote: ... the phages were also precipitated by setting the suspension to 0.5 M NaCl and 10 % (w/v) polyethylene glycol (PEG) 6000 (Sigma-Aldrich, Taufkirchen, Germany). Precipitated phages were pelleted with 14,000 g for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... Fertilized embryos were injected before first cleavage on agarose pads in a solution of pasteurized (30 minutes 65°C) filtered seawater (PFSW) + 2% w/v Ficoll 400 (Sigma F-9378). Embryos were hand-sorted for fluorescence between second and sixth cleavage cycles ...
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bioRxiv - Plant Biology 2020Quote: ... Fixed plant material was digested at 37 °C in the PINE enzyme mixture consisting of 2 % (w/v) cellulase from Aspergillus niger (Sigma C-1184), 4 % (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 200 μL 1X Annexin V binding buffer + 2 uL of 100 μg/mL propidium iodide (PI) (Sigma-Aldrich, #P4170). Data acquisition was performed on a BD FACS Canto II Flow cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with HBSS before incubating cells with EV isolation media (MesenCult™ MSC Basal medium containing 0.5% (w/v) bovine serum albumin (BSA, Sigma-Aldrich, Australia) for 48 h ...
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bioRxiv - Biophysics 2021Quote: ... The samples were washed with PBS three times and then blocked in PBS with 5% (w/v) bovine serum albumin (BSA, Sigma-Aldrich A3059) overnight at 4°C.
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bioRxiv - Microbiology 2020Quote: Crystal violet stain that had incorporated into the biofilm was re-solubilised upon the addition of 150 μL of 1% sodium dodecyl sulphate (SDS) (w/v) (Sigma-Aldrich, USA).
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bioRxiv - Biophysics 2019Quote: ... embedded fish were submerged in E3 with .008% w/v Tricaine and 50 mM Hepes plus 12 μm Rockout (Sigma Aldrich #555553). For paired control/Rockout trials ...
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bioRxiv - Cell Biology 2021Quote: ... tissue culture plates were precoated for 30 min at 37°C with 0.1% (w/v) porcine gelatin (Sigma, St. Louis, Missouri, US) in 1X PBS (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... and perfused with 40 ml of 0.1 M/L phosphate-buffered saline and 40 ml of 4% (w/v) PFA (Sigma, 30525-89-4) 24h after social interaction ...
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bioRxiv - Biophysics 2021Quote: Mica was freshly cleaved and then pre-treated by depositing 20 μl 0.01% (w/v) 1-5 kDa poly-L-lysine (Sigma-Aldrich, MO, USA), left for 5 mins before washing with 1000 μl filtered milliQ H2O and finally vacuum drying ...
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bioRxiv - Immunology 2022Quote: ... Membranes were blocked in PBS-T or TBS-T complemented with 5 % (w/v) bovine serum albumin (BSA, Sigma-Aldrich, A3294-50G) for up to 3 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were diluted in [3 % Bovine Serum Albumin (BSA) fraction V (Fisher, BP1600-100) in PBST and 0.1 % Sodium Azide NaN3 (Sigma Ultra, s-8032)] as follows ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were then diluted to a final concentration of 1% (w/v) SDC using water and digested for 16 h with trypsin 1:50 (w/w) (Sigma Cat# T6567) at 37°C at 1000 rpm in a Thermomixer-C (Eppendorf) ...
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bioRxiv - Immunology 2022Quote: ... 2 x 106 cells/ml were incubated for 24 h with 200 μg/ml ovalbumin protein (OVA, Grade V, Sigma, St.Louis, MO). Prior to being added to cultures ...