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Citations for Millipore Sigma :
7151 - 7200 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... magnetic force was applied by placing the assay plates on a handheld magnetic separation block (V&P Scientific 771HH-H/Millipore 40-285). In all subsequent experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Electrophoresis was run at 120 V for 50 min in 1x tris-glycine-SDS running buffer [25 mM tris base (Sigma, T1503-1KG), 190 mM glycine (Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 0.2% (w/v) fatty acid-free BSA were seeded in poly(ethyleneimine)-coated (1:15,000; Sigma-Aldrich, Cat. no: 03880) XFe96 seahorse plates by centrifugation at 2,000 g for 18 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Mice received an intraplantar injection (unilateral or in both hind paws) of 5 μl λ−carrageenan (primed, 1% w/v, Sigma-Aldrich) to induce transient inflammatory hyperalgesia ...
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bioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized and transcardially perfused with ice-cold PBS (1x) followed by 4 % paraformaldehyde in PBS (PFA, w/v, pH 7.4, Sigma Aldrich, Taufkirchen, Germany). The brains were post fixed in 4 % PFA over night at 4°C and cryoprotected in 30 % sucrose (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... Imaging acquisition was performed in fresh imaging buffer (50 mM Tris-HCl, pH 8.0, 10 mM NaCl, 10% (w/v) glucose (Sigma-Aldrich, cat#G8270), 1.5 mg MEA (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Overnight cultures of the desired bacterial strains were prepared in microtiter plates using 200 µL of TSB supplemented with 1 % (w/v) glucose (Sigma-Aldrich, UK) to facilitate biofilm formation (Croes et al. ...
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bioRxiv - Bioengineering 2024Quote: ... gelatin was dissolved in 10 w/v% sodium carbonate– bicarbonate (CB) buffer (0.25 M, from Sigma Aldrich, pH was adjusted to 9) at 50 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with PBS and permeabilized at RT for 20 minutes using 0.5% w/v saponin (Sigma Aldrich, Cat # 47036), 0.5% v/v Triton X-100 (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: Data in Figure 1b and part of Figure 4a were collected using an aqueous ∼3.5 mM solution of linoleic acid conjugated to 10% w/v BSA purchased from Sigma (cat no. L9530), approximately a 2:1 FA:BSA ratio ...
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bioRxiv - Microbiology 2024Quote: ... we prepared our own fatty acid mixtures by conjugating them to a stock solution of100 mg/mL (10% w/v) BSA in PBS (Sigma A9418-10G). Fatty acids were purchased from Nu-Chek Prep (linoleic acid ...
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bioRxiv - Cell Biology 2023Quote: ... cover slips were washed with ice cold PBS and BMDMs were fixed in 4% (w/v) paraformaldehyde (PFA) (in PBS) (Sigma-Aldrich, P6148) for 15 minutes at RT ...
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bioRxiv - Biophysics 2023Quote: ... σ70RNAP549 holoenzyme was introduced into the chamber at 1 nM in transcription buffer (wash buffer supplemented with 3.5% w/v PEG 8,000 (#81268; Sigma-Aldrich; St. Louis, MO), 1 mg mL−1 BSA ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed three times with DPBS+/+ and then infected with a multiplicity of infection (MOI) 5 in DMEM containing 0.2 % (w/v) Bovine Serum Albumin (BSA; Sigma Aldrich, Taufkirchen, Germany), 2 mM L-glutamine ...
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bioRxiv - Molecular Biology 2023Quote: ... The integrity (DNA fragment size) of extracted DNA was evaluated using a 1% (w/v) agarose gel electrophoresis (Sigma-Aldrich, United Kingdom) in 1⊆ tris-acetate-EDTA (TAE ...
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bioRxiv - Biochemistry 2023Quote: ... dried phosphopeptides from each fraction were re-dissolved in 800 μL cold IAP buffer (50 mM Tris-HCl, 150 mM NaCl, 1% w/v n-Octyl-β-D-glucopyranoside (EMD Millipore), EDTA-free Halt protease inhibitor and phosphatase inhibitor cocktails ...
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bioRxiv - Bioengineering 2023Quote: ... The staining solution was composed of culture medium containing the red dye Erythrosin B (0.1% w/v; Sigma-Aldrich; product number: 200964). The staining solution was left to perfuse or diffuse tissue constructs following gravity ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Microbiology 2023Quote: ... cultures were grown in 4% w/v clarified V8 broth supplemented with 30 µg/ml β-sitosterol (Sigma-Aldrich, St. Louis, Missouri) and incubated in the dark at 22 °C for 4 to 6 weeks ...
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bioRxiv - Neuroscience 2023Quote: ... 40µm coronal brain cryosections were collected at −18°C and stored as free-floating sections in 0.01% w/v sodium azide (Sigma-Aldrich, cat. #S2002) in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... and ABTS substrate solution (water containing 0.54mg/mL w/v 2’2-Azinobis (3-ethylbenzthiazoline Sulfonic Acid) diammonium salt (Sigma-Aldrich, Cat#A-1888), and 0.1M Citric Acid and 0.03% v/v Hydrogen Peroxide (H2O2) ...
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bioRxiv - Physiology 2023Quote: Mice were lightly and briefly (< 5 min) anesthetized with 2% isoflurane and injected with 40% w/v PEG-8000 (Sigma-Aldrich, 89510) in sterile 0.9% NaCl subcutaneously using a 28G insulin syringe ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then gently washed three times with Tris-buffered saline (TBS)-Tween (150 mM NaCl, 50 mM Tris-HCL buffer and 1% w/v Tween20) (TBS; EMD Millipore 524750, Tween20; Sigma-Aldrich) and incubated in Alexa Fluor 680 Donkey anti-goat secondary (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: Zebrafish larvae were anesthetized and immobilized in a lateral orientation in 1.25% (w/v) low gelling temperature agarose (Millipore Sigma, #A9414-25G) dissolved in fish water ...
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bioRxiv - Biochemistry 2024Quote: ... cells were supplemented with high glucose DMEM containing 100 µM 4-HDHA conjugated to 1% fatty acid-free BSA (w/v) (Sigma-Aldrich; A6003). Cells were cultured with 4-HDHA for 24 hours prior to harvest ...
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bioRxiv - Cell Biology 2024Quote: Tissues were dissected using a razor blade and fixed with 4% (m/v) paraformaldehyde (PFA) solution in nuclease-free 1xPBS buffer (pH8.0, Sigma-Aldrich, Cat.# AM9624). Following fixation ...
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bioRxiv - Genomics 2023Quote: ... The tissue was enzymatically digested on a MACSMix rotator (set to 16 rpm speed) at 37°C in pre-warmed RPMI/FBS containing Collagenase V (Sigma-Aldrich, C9263), and DNAse I (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... The gel pieces were dehydrated in 100 % (v/v) ACN and then rehydrated with 30 µL of 3.33 µg × mL-1 trypsin (cat-no. T6567 from Sigma-Aldrich/Merck, www.sigmaaldrich.com) in 25 mM ABC ...
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bioRxiv - Microbiology 2024Quote: ... The spleen cells were separated and fused with SP2/0 myeloma cells three days later by 50% (w/v) PEG4000 (Sigma-Aldrich, USA). PCV4-VLP-ELISA screened the antibodies in supernatants of hybridoma ...
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bioRxiv - Microbiology 2024Quote: The pads are made by mixing the desired combination of growth media and any temperature-stable constituents with 1% w/v agarose powder (Sigma-Aldrich, A9539). The agarose mix is then heated to 80 °C to fully dissolve the agarose powder and cooled to 60 °C before adding temperature-sensitive constituents such as antibiotics ...
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bioRxiv - Microbiology 2024Quote: ... The crystal violet stain incorporated into the biofilm was re-solubilized by adding 900 µL of 1% sodium dodecyl sulfate (SDS) (w/v, Sigma-Aldrich, USA), followed by a 10 min incubation at room temperature in the dark ...
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bioRxiv - Cell Biology 2024Quote: mESC line ES-E14TG2a (ATCC; Cat #CRL-1821) was cultured on plastic dishes precoated with 0.1% (w/v) Gelatin (Sigma; Cat #G2500-100G) in Serum/LIF medium ...
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bioRxiv - Bioengineering 2024Quote: ... hydrogels were fixed in 4% PFA for 45 minutes at room temperature then transferred into a 30% w/v sucrose solution (Sigma Aldrich, #S0389) and left for 24 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by an over night incubation at 37°C in a slide hybridizer machine (ACD HybEZ™ II Hybridization System) with hybridization buffer (10% deionized formamide; 2X SSC; 10% w/v Dextran sulfate, Sigma-Aldrich, D8906 ...
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bioRxiv - Neuroscience 2024Quote: ... at molar ratio of 4:2:2 was prepared by dissolving the lipids to a final concentration of 10 mg/mL in ethanol containing 2.3 % Span 80 w/v (Millipore Sigma cat# S6760). The lipid:RNA volume ratio was 3:1 ...
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bioRxiv - Biochemistry 2024Quote: ... the pellet was resuspended in 100 µL of a 10% (w/v) TCA solution and broken for 10 min with glass beads (Sigma-Aldrich, #G8772) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Then 8 µL samples were spotted on a silicon disk coated with 0.01% (w/v) poly-L-lysine (Merck Millipore, Billerica, MA, USA). Cells were fixed with 2.5% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... and triturated before plating at 250,000 cells/well on poly-L-lysine-treated coverslips (Sigma #P2636) in 24-well plates ...
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bioRxiv - Cell Biology 2020Quote: ... lentiviruses as well as desired shRNA viruses in NPC media containing 10 μM Thiazovivin (Millipore, #S1459) and spinfected (centrifuged for 1 hour at 1000g) ...
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bioRxiv - Microbiology 2021Quote: ... β-actin as well as NP were detected using monoclonal anti-β-actin (Sigma-Aldrich, USA) as well as polyclonal-anti-NP rabbit antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... wells were washed twice with PBS and subsequently coated with 2 µg/ml laminin (Sigma, L2020) in PBS overnight at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR as well as oligonucleotides used for biophysical studies were synthesized either by Sigma-Aldrich (USA). or by GCC Biotech (India) ...
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bioRxiv - Bioengineering 2022Quote: ... and 4 μL of nuclease-free water in each well on the 1.2% agarose gel (Sigma) stained with Ethidium Bromide (1 mg/μL ...
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bioRxiv - Microbiology 2019Quote: ... Medium was then removed from the wells and replaced with medium containing 100µg/ml gentamycin (Sigma) to kill extracellular bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... Each well contained 150 μL culture and covered with 50 μL of mineral oil (Sigma-Aldrich) to avoid evaporation ...
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bioRxiv - Bioengineering 2020Quote: ... wells were blocked with blocking buffer (PBS + 0.05% Tween 20 (PBST) containing 5% skim milk (Sigma)) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The wells were washed with three times with PBS and blocked with 1% BSA (A9647, Sigma) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Streptavidin conjugated with horseradish peroxidase (HRP) was added to each well (1:5,000, Cat S2438, Sigma). After incubation at RT for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... and adenine as well as 0.05 μg/mL of thiamine (#U0750, #H8000, #A9126, #T4625, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... wells were washed five times with 200uL PBS containing 0.1% Tween20 (Sigma-Aldrich, St. Louis, MO). After the final wash ...