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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... Cellular proteins were separated by SDS-PAGE and transferred to a PVDF membrane (Millipore IPVH00010) using standard protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were purified from hybridoma culture supernatant using protein G Sepharose 4 Fast Flow (Sigma). Injection of the respective antibody resulted in depletion of >90% of CD4+ or CD8+ T cells for at least 14 days ...
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bioRxiv - Molecular Biology 2020Quote: ... The bound proteins were neutrally eluted with 50 µL of V5 peptide (V7754-4MG, SIGMA) at 1mg/mL in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 stocks were additionally cleared using a 0.45 μM low protein binding filter (Millipore) to remove mucus debris produced by these cells and the medium was exchanged three times for Opti-MEM I (1X ...
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bioRxiv - Immunology 2022Quote: ... The alkylation of the proteins was carried out by adding chloroacetamide (Sigma-Aldrich, Saint-Louis) to a final concentration of 7.5 mM followed by a 20 minute incubation in the dark at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Proteins in supernatants were then precipitated by incubating with 0.61 N trichloroacetic acid (Sigma-Aldrich) plus 1× protease inhibitory cocktail (PIC ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition of protein A/G beads (10 ul suspension; PureProteome, EMD Millipore) for an additional 5 h on an orbital plate ...
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bioRxiv - Immunology 2022Quote: ... Bound proteins were eluted by incubating the beads with 200µg/ml 3xFLAG peptide (Sigma- Aldrich) in IPP150 containing 0.02% NP-40 ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were separated by SDS-PAGE and transferred onto Immobilon-PSQ membrane (0.2 µm; Millipore). Immunoblotting was performed using the following primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA binding proteins including target RNAPs were precipitated by addition of polyethyleneimine (PEI, Sigma-Aldrich) to 0.6% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein was degraded by adding 2 µL 18.2 mg/mL Proteinase K (Millipore Sigma 3115887001) and incubating at 55°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were rinsed in milliQ water and protein transfer was checked by Ponceau staining (Sigma). Transferred membranes were washed once with TBS and three times with TBS-Tween (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were transferred onto a buffered poly-vinilidine di-fluoride (PVDF) membrane (Millipore, Germany). The non-specific staining was blocked by 5% skimmed milk protein (1X TBST;4hr ...
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bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
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bioRxiv - Biochemistry 2022Quote: ... protein was concentrated in an Amicon Ultra-15 30 kDA centrifugal filter units (Millipore #UFC9030) and incubated overnight at 4 °C with His-tagged PreScission protease (purified in house ...
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bioRxiv - Biochemistry 2022Quote: hnRNPDL proteins were detected using different primary antibodies (anti-hnRNPDL antibody, HPA056820 from Sigma-Aldrich or with an anti-GFP monoclonal antibody ...
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bioRxiv - Biochemistry 2022Quote: ... 0.1% TritonX-100) containing protease and protein phosphatase inhibitor cocktails (Sigma Aldrich, Gillingham, Dorset, UK). Protein concentrations were measured with BCA protein assay and samples were boiled for 5 min at 90°C after addition of 5%β-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins bands were immobilized through transfer to PVDF membrane (IPFL00010, Immobilon Transfer Membrane, Millipore, Ireland) for one hour at 110 volts in ice ...
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bioRxiv - Cell Biology 2022Quote: ... the enzymatic activity derived from the fusion protein was detected using NBT/BCIP substrate (Sigma). For competition binding ...
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bioRxiv - Genomics 2022Quote: mECSs harboring a homozygous endogenous NELFB-FKBP12F36V fusion protein were cultured on 0.1% gelatin (Millipore) in PBS+/+ coated tissue-culture grade plates ...
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bioRxiv - Plant Biology 2022Quote: ... Elution of immunoprecipitated proteins was performed using 100 μg/mL c-Myc peptide (Sigma, M2435), on ice for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... After adding 1x protein loading buffer (42526.01, SERVA) containing 5% ß-Mercaptoethanol (M6250, Sigma-Aldrich), protein samples were denatured 5 min at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were concentrated using 3 kDa MWCO Amicon Ultra-0.5 Centrifugal Filter Units (Merck Millipore). Protein concentrations were determined using the Proteins and Labels function on a Thermo Scientific™ NanoDrop™ One Microvolume UV-Vis Spectrophotometer and subsequently confirmed on SDS-PAGE gel upon Coomassie staining and colorimetric imaging on an Amersham ImageQuant 800 CCD Imager.
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies for immunofluorescence included rabbit anti-glial fibrillary acidic protein (GFAP) (EMD Millipore; AB5804), rabbit anti-microtubule associated protein-2 (MAP2 ...
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bioRxiv - Plant Biology 2022Quote: Protein samples were run on SDS-PAGE and transfer onto PVDF membrane (Immobilon-P, Millipore). The membrane was blocked by PVDF blocking reagent (Toyobo ...
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bioRxiv - Microbiology 2023Quote: ... Co-immunoprecipitated proteins were eluted for 1h at 4°C with 3X FLAG peptide (Sigma) in TBS (pH 7.4) ...
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bioRxiv - Physiology 2022Quote: ... the University of Michigan Transgenic Core injected Cas9 protein (Sigma-Aldrich, St. Louis, MO, USA), editing templates containing LoxP sites (IDT Ltd. ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Protein loading quantity was controlled using mouse monoclonal anti-α-tubulin antibodies (Sigma, Cat# T5168).
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ECL Western blotting Analysis System was used to reveal protein bands (Millipore, United States). Densitometry was performed with ImageJ software and standardized against β-actin.
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation of FLAG fusion proteins was performed using an anti-FLAG M2 affinity gel (Sigma) by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein levels were determined by immunoblotting using anti-FLAG clone M2 (Sigma, F3165, 1:3000).
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bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... eluted proteins were then concentrated using amicon ultra-15 centrifugal filters (Cat# UFC901024, Merck Millipore).
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Supernatant was used as input fractions ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatants were transferred into fresh tubes and proteins concentrations were assessed using Bradford assay (Sigma). Sample were then mixed with 4x SDS buffer and boiled for 95°C for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... 40 μg protein per sample was alkylated for 30 min with 20 mM Iodoacetamide (Sigma) and digested with 5% trypsin (w/w ...
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bioRxiv - Plant Biology 2024Quote: ... The DDB2-FLAG fusion protein was detected using the anti-FLAG HRP (Sigma-Aldrich: A8592) at a 1:5,000 (v ...
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bioRxiv - Neuroscience 2024Quote: ... Protein levels were visualized by chemiluminescence using either Immobilon Chemiluminescent HRP substrate ECL (Millipore #WBKLS0500) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher #34095) ...
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated using Amicon 10K and 30K columns (Millipore, Ref. UFC803096; UFC801096) until the final protein concentration reached approximately 1 to 2 mg/mL ...
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Physiology 2024Quote: ... viral media was collected and passed through a 0.45 μM low-protein binding filter (Millipore) and concentrated by low-speed centrifugation (3300 g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins bands were immobilized through transfer to PVDF membrane (IPFL00010, Immobilon Transfer Membrane, Millipore, Ireland) for 90 min at 30 volts in ice ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... All samples were diluted to the same protein concentration using 2X Laemmli buffer (Sigma-Aldrich), boiled for 10 minutes ...