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Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biophysics 2020Quote: ... The purified protein was incubated and for 1h at 37°C with bovine thrombin (Sigma) in a 1000:1 molar ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (16-663; Millipore) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... fractions containing pure protein were concentrated using Amicon Ultra 10 kDa MWCO filters (Millipore Sigma), dialyzed into PBS ...
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bioRxiv - Biochemistry 2020Quote: N-protein was dyed by adding (3:1) Atto 488 NHS ester (Millipore Sigma 41698) to purified protein and incubating mix at 30°C for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... was used for blotting and proteins were stained using the following antibodies: (Sigma-Aldrich AV46863), TMEM41B (Sigma-Aldrich HPA014946) ...
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bioRxiv - Biophysics 2020Quote: ... Protein was eluted with Myosin Buffer supplemented with 100 μg/mL Flag peptide (Sigma-Aldrich). The eluent was buffer-exchanged to Storage Buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The amount of total protein in the samples were quantified using the Bradford assay (Sigma). An equal concentration of protein samples was mixed with 2X Laemmli buffer and the samples were boiled for 10 min at 100° C ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein-antibody complex was visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Molecular Biology 2022Quote: ... and the ATAD5-RLC proteins were eluted with 100 µg/mL FLAG-peptide (Sigma Aldrich) in the same buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... protein was transferred from the 20% polyacrylamide gels to PVDF Immobilon-P membranes (Millipore Sigma) and blocked with 5% (w/v ...
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bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
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bioRxiv - Immunology 2022Quote: ... After that 50 µl of 1:5 dilution Protein A colloidal gold conjugate (Sigma, USA), which is used as an indicator was added to the test hole and was allowed to soak for 2 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were transferred into new tubes and protein concentration were determined by BCA assay (Sigma). Approx ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto a nitrocellulose membrane (Sigma-Aldrich). After incubation with the appropriated primary ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was concentrated to 1 mg/ml using a 50,000 MWCO Amicon Concentrator (Millipore), flash frozen and stored at -80°C.
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bioRxiv - Biochemistry 2022Quote: ... proteins separated by SDS-PAGE were transferred to a PVDF membrane (Immobilon-P, Merck Millipore) in a Mini Trans-Blot (Bio-Rad ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... the proteins on the gel were transferred to Immobilon PVDF membranes (EMD Millipore, Billerica, MA) at 400 mA for 2.5 hrs ...
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bioRxiv - Physiology 2022Quote: Protein samples were solubilized in 1% NP-40 lysis buffer with 4% protease inhibitors (Sigma) and centrifuged for 14,000 g for 5 minutes ...
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bioRxiv - Neuroscience 2019Quote: Antibodies used were: rat anti-myelin basic protein (MBP, 1:200, MAB386, Millipore, MA, USA), rabbit anti-Olig2 (1:200 ...
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bioRxiv - Cancer Biology 2019Quote: ... The DTT and IAA treated proteins were then dialyzed against 50 mM ammonium bicarbonate (Sigma) for 24h at 4C and with the dialysis buffer changed three times ...
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bioRxiv - Plant Biology 2020Quote: ... Solubilized proteins were incubated with 15-20 µl of Anti-FLAG M2 Affinity Gel (Sigma) for 2 h with gentle rotation at 4°C ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Microbiology 2019Quote: Protein samples were separated by SDS-PAGE and blotted on PVDF (polyvinylidenfluoride) membranes (Merck Millipore). Membranes were blocked for 1h with PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Firefly luciferase activity was normalized to protein content using Bicinchoninic Acid Kit from Sigma-Aldrich® and measured with using a luminometer (Centro XS3 LB960 ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2019Quote: ... incubated with mouse α-anti-HA clone 12CA5 conjugated with protein G beads (Sigma-Aldrich) for 16 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The proteins were removed using Amicon Ultra 3K 0.5 ml centrifugal filters (Millipore, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... buffer exchanged and protein dialysis was performed using Amicon Ultra-4 centrifugal concentrators (UFC801008, Millipore,) with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were transferred into methanol pre-activated polyvinylidene fluoride (PVDF) membranes (Immobilon-P, Merck-Millipore). After transference ...
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bioRxiv - Immunology 2019Quote: ... Protein aliquots were reduced with 5 mM tris-2-carboxymethyl phosphine (TCEP) buffer (Sigma Aldrich) and incubated for 1 hour at 60°C to reduce disulfide bonds ...
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bioRxiv - Plant Biology 2020Quote: Sample proteins were separated by SDS-PAGE and electrotransferred onto an Immobilon-P membrane (Millipore) for immunoblot detection ...
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bioRxiv - Immunology 2019Quote: ... Eluted proteins were concentrated using Amicon Ultra 0.5ml Centrifugal Filter tubes NMWL 30 KDa (Millipore) and quantified by NanoDrop Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein were separated by 8% SDS-PAGE and transferred to a polyvinylidene fluoride membrane (Millipore). Then ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were separated by SDS-PAGE and transferred onto a nitrocellulose filter membrane (Millipore, USA). The membranes were incubated with primary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and trypsin (porcine pancreas, Type IX-S, 13000-20000 BAEE units/mg protein, Sigma-Aldrich) stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES ...
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bioRxiv - Synthetic Biology 2020Quote: ... These pellets were resuspended and lysed in 100 µL of Bugbuster protein extraction reagent (Millipore) containing 5 U mL−1 Benzonase nuclease (Millipore ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were transferred from gels to Immobilon-P polyvinylidene difluoride 0.45 μm membranes (Millipore, USA) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins samples were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore) for 16 h at 100 mA ...
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bioRxiv - Molecular Biology 2019Quote: RNA-renatured proteins (~40 μg) were supplemented with 1 μg anti-NF-H (N4142, Sigma), 1 μg anti-Tau (T9450 ...
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bioRxiv - Systems Biology 2019Quote: ... Expression of the CARD-CC proteins was determined with anti-Flag antibody (F-3165, Sigma). Expression of the E-tagged PKC clones with anti-E-tag antibody (ab66152 ...
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bioRxiv - Biophysics 2020Quote: ... We collect the supernatant and precipitate proteins by adding 300 mg/mL ammonium sulfate (Sigma). We stir the solution for 30 minutes at room temperature and centrifuge again at 12,000 rpm for 0.5 hour ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluted proteins were precipitated with the addition of 0.25x volume of 100% trichloric acid (Sigma). Protein pellets were washed five times with cold Acetone (Merck ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) with the mouse anti-Ago2 antibody (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was then concentrated to 1 mg/ml using Amicon device 30K (Merck Millipore) and stored at -80°C.
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bioRxiv - Molecular Biology 2020Quote: ... coli ProQ protein or its N-terminal domain (NTD) were cloned into pET15b vector (Novagen) using BamHI restriction site (Supplemental Table S2) ...
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bioRxiv - Biophysics 2019Quote: ... followed by on-matrix cleavage of the protein using 50 units of bovine thrombin (Sigma) per litre of culture at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220, Sigma Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-Sepharose (P3391) and PRMT5:MEP50 active complex (SRP0145) were purchased from Sigma Aldrich. Protein co-immunoprecipitation was performed using ANTI-FLAG® M2 Affinity Gel (A2220 ...
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bioRxiv - Molecular Biology 2021Quote: ... The following commercially available antibodies were used to detect targeted proteins: anti-SPF45 (Sigma-Aldrich), anti-SF3b155 (MBL Life Science) ...