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Citations for Millipore Sigma :
6751 - 6800 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-P or Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in 5% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto methanol-preactivated Immobilon-P PVDF membranes (pore size 0.45 μm; Millipore) using a semi-dry transfer cell or a wet transfer tank (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... The sypro orange protein gel stain was purchased from Sigma Aldrich (Merck KGaA, Darmstadt, Germany). The integrated potato culture medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred from the polyacrylamide gel to a PVDF membrane (Merck Millipore, Massachusetts, USA). Membranes were blocked with Rockland blocking buffer (Biomol GmbH ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with endoproteinase Lys-C (Wako Chemicals) and sequencing grade-modified trypsin (Sigma). Protease digestion was stopped by addition of trifluoroacetic acid to 0.5% and precipitates were removed by centrifugation ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... RIPA buffer was not used and the protein concentration was measured with Bradford assay (Millipore).
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bioRxiv - Biophysics 2020Quote: Hen egg white Lyz and bovine β-Lac proteins were both purchased from Sigma-Aldrich and used with no further purification ...
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bioRxiv - Biophysics 2020Quote: ... Protein was concentrated and buffer exchanged on Amicon Ultracel-10K centrifugal filters (Millipore; Darmstadt, Germany), dialyzed exhaustively into 10 mM MOPS ...
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bioRxiv - Cancer Biology 2021Quote: ... 80 μg protein extract from each sample was reduced with 50 mM dithiothreitol (Sigma, #43815) at 95 °C for 10 min and then alkylated with 100 mM iodoacetamide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Equal amount of protein was loaded to the anti-Flag M2 Magnetic beads (M8823, Sigma) and anti-HA Magnetic beads (88836 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by SDS-PAGE (8% acrylamide gel) and transferred to nitrocellulose membranes (Millipore). Membranes were incubated overnight at 4°C with a primary antibody diluted in Tris-buffered saline plus 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2021Quote: ... The dialysed protein was concentrated in a 10 kDa cut off Amicon Ultra-15 (Millipore), quantitated using BCA ...
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bioRxiv - Biophysics 2022Quote: ... The protein samples were concentrated to 30mg/ml using 100-kDa MWCO AmiconUltra concentrators (Millipore) for crystallization trials and further analysis.
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bioRxiv - Cancer Biology 2022Quote: ... and then the proteins were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA, USA).
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bioRxiv - Biochemistry 2022Quote: WT and mutant Streptococcus pyogenes Cas9 proteins were expressed in E.coli Rosetta 2 (DE3) (Novagen) for 16 hours at 18 °C as fusion proteins with an N-terminal His6-MBP-TEV tag ...
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bioRxiv - Pathology 2022Quote: ... Proteins separated via SDS-PAGE gels were blotted onto PVDF membranes (Millipore, Cat. No. ISEQ00010). To reduce non-specific antibody binding and reduce background ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Biochemistry 2022Quote: ... protein samples were subjected to 10% SDS-PAGE and moved to PVDF films (Millipore, America). After the blockade with 5% milk ...
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bioRxiv - Biochemistry 2022Quote: ... Purified protein was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 50 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: ... the eluted proteins of IP were transferred to a 10 kD centrifugal spin filter (Millipore), and followed by washing with 8 M urea 200 μL for three times ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins were separated by 15% SDS-PAGE gel and blotted onto a nitrocellulose membrane (Millipore). The membrane was first stained with Ponceau S to show protein loading before blocking and incubation with first antibodies ...
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bioRxiv - Pathology 2022Quote: ... XO Activity was expressed as units/mg protein using native XO from buttermilk (Merck Millipore) as standard.
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bioRxiv - Pathology 2022Quote: ... 15-30 μg protein per sample were denatured with 5 % vol/vol 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt™ 4–12 % Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Pathology 2022Quote: ... Equal amounts of proteins were incubated with anti-FLAG M2 affinity gel (Sigma Aldrich, A2220) and processed by Western blotting.
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bioRxiv - Genetics 2022Quote: ... 200 μg of protein extract was incubated with 30 μl anti-HA magnetic beads (Sigma) at 4°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein solution was applied to a MonoQ 10/100 column (Sigma-Aldrich, Merck), equilibrated in MonoQ buffer A (40 mM Hepes ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein extract was loaded onto the centrifugal filter CO10 kDa (Merck Millipore, Darmstadt, Germany), and detergent were removed by washing five times with 8M Urea (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were electrophoretically separated using 10% SDS gel and transferred onto nitrocellulose membrane (Millipore, USA). The membranes were blocked with 5% Non-Fat Dry milk in PBST (1X PBS with 0.1%Tween-20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein extracts from tumor tissues were prepared by homogenization in RIPA buffer (Sigma-Aldrich, USA) containing a protease inhibitor cocktail (Roche ...
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bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was then reduced with tris(2-carboxyethyl)phosphine (TCEP, obtained from Sigma), a strong ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... The proteins were then transiently transfected (pBiex-1; Escort IV, Sigma-Aldrich, St. Louis, MO) into Sf9 cells ...
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bioRxiv - Cancer Biology 2022Quote: Total protein extracts were obtained from cell lines and tumour specimens using RIPA (Sigma-Aldrich) buffer and subjected to SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies (listed in Table S4) were pre-conjugated to magnetic protein A/G beads (Millipore). Chromatin immunoprecipitation was carried out overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were stained with Ponceau S (0.1% Ponceau reagent [Sigma, P3504] with 5% acetic acid). Incubation with primary antibodies was conducted overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... to be subsequently treated with Iodacetamide for protein alkylation (Sigma-Aldrich, St. Louis, MO, USA) and ultimately digested with 1.25 µg of recombinant Trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were reduced with 60 µL 40 mM dithiothreitol (DTT; Sigma-Aldrich, Taufkirchen, Germany) in 50 mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... protein extracts were incubated for 45 min with 1%v/v Benzonase Nuclease (SIGMA E1014). For the FASP digestion ...
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bioRxiv - Cell Biology 2021Quote: ... crosslinked to Protein A/G PLUS-Agarose (SantaCruz) using 20 mM dimethyl pimelimidate (Sigma Aldrich) at 4°C for overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... protein was dialyzed into REMSA buffer [10mM HEPES pH 7.3 (Sigma-Aldrich, Cat. No. H3375), 20 mM KCl (Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then electro-transferred onto a polyvinylidene fluoride (PVDF) membrane (0.2 µm; Millipore). After blocked with 5 % skim milk in TBST (50 mM Tris-HCl ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Microbiology 2021Quote: ... After preclearing the cell lysate with protein A/G agarose magnetic beads (#16-663, Millipore) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Chloroquine diphosphate (C6628) and HRP-conjugated Protein G (#18-160) were purchased from Sigma-Aldrich. Torin1 (#4247 ...
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bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... separated protein lysates were transferred onto polyvinylidene difluoride membranes (Immobilon-P; EMD Millipore, Burlington, MA); membranes were blocked in 5% BSA (BSA Fraction V ...
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bioRxiv - Neuroscience 2021Quote: ... and the proteins denatured by adding 200 μL of 8 mol/L urea (Sigma-Aldrich), followed by 15 min sonication ...