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Citations for Millipore Sigma :
7001 - 7050 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The cleaved protein’s peak fractions were pooled and concentrated using 50kDa cut-off Amicon (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane (#IPVH00010, Millipore).
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was pre-treated with EZview Red Protein A agarose beads (P6486; Sigma) for 45 minutes to reduce the likelihood of nonspecific binding to the agarose ...
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bioRxiv - Cell Biology 2022Quote: Proteins from GL261 and CT2A cells were extracted in RIPA buffer (Sigma, cat# R0278-50mL) containing a protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and the lysates were immunoprecipitated with specific antibody and protein A/G-Sepharose (Sigma-Aldrich) at 4 °C for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein was extracted using radioimmunoprecipitation (RIPA) assay buffer (Cat # R0278, Sigma-Aldrich, St. Louis, MO) containing cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... The expressed protein was purified using Glutathione Sepharose 4B (Sigma-Aldrich Cat# GE17-0756-01) using the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Western blotting of immunoprecipitated proteins was performed as described above using anti-FLAG (F1804, Sigma) or anti-HA (11867423001 ...
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bioRxiv - Biochemistry 2023Quote: ... and protein thereafter eluted with Strep-buffer supplemented with 2.5 mM d-desthiobiotin (Sigma-Aldrich). All purified proteins were concentrated to appropriate concentration using 30 kDa Mw cut-off centrifugal filters (Amicon ...
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bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred to PolyVinyliDene Fluoride 0.45 µm membranes (Immobilon-Fl, Merck Millipore, Darmstadt, Germany). Membranes were saturated 1 hour with a Tris-Buffered Saline blocking solution (TBS blocking solution ...
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bioRxiv - Physiology 2023Quote: ... 15-35 µg protein per sample were denatured with 5 % v/v 2-mercaptoethanol (Sigma) in LDS sample buffer (Thermo ...
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bioRxiv - Zoology 2023Quote: ... 10 μl of supernatant was used for protein quantification using Bradford Reagent (Sigma, B6916-500ML). Whole-body uric acid levels were measured from 5 μl of supernatant at 22°C for 30 min using uric acid assay kit following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was purified using the HiPrep Q Fast Flow 16/10 column (Sigma-Aldrich) followed by the reverse-phase HPLC C4 column ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... Isolated proteins were then segregated by gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore). Membranes were subsequently incubated in primary antibodies (Supplementary Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... Purified proteins were further concentrated and changed to PBS buffer using the Centricon device (Millipore).
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bioRxiv - Neuroscience 2023Quote: Total protein samples were lysed in radioimmunoprecipitation (RIPA) buffer containing 2% protease inhibitor cocktail (Sigma). Protein quantification was completed using the Pierce Bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were separated by 10% SDS-PAGE and transferred to the PVDF membrane (Millipore, USA). The membrane was placed in 5% bovine serum albumin and incubated with the anti-midP0188 pAb (1:500 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... TEV digested protein was concentrated to < 2 mL using an Amicon Ultra-15 concentrator (Millipore) and then purified by size exclusion chromatography ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 1 h incubation with Protein G agarose beads (Millipore, catalog no. 16-266) at 4 °C with constant rotation ...
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bioRxiv - Plant Biology 2023Quote: ... antibody for the OsExo70F3 protein in pGBKT7 and with the anti-GAL4 activation domain (Sigma) antibody for AVR-Pii and ZiF effectors in pGADT7.
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bioRxiv - Neuroscience 2023Quote: ... The eluted proteins were loaded into Amicon® Ultra-15 centrifugal filter unit (Millipore Sigma), washed with Tris buffer ...
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Molecular Biology 2024Quote: ... The dialyzed protein was concentrated using Amicon 10K and 30K columns (Millipore, Ref. UFC803096; UFC801096) until the final protein concentration reached approximately 1 to 2 mg/mL ...
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bioRxiv - Physiology 2023Quote: Protein was measured using Bradford’s method according to manufacturer orientation (Millipore-Sigma, #1103060500, ON-Canada).
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bioRxiv - Cell Biology 2024Quote: ... FLAG-proteins were eluted with 50 µL 500 ng/mL 3xFLAG peptide (Sigma, Cat. # F4799) at RT for 1h and supplemented with 5x SDS sample buffer (250 mM Tris-HCl pH 6.8 ...
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bioRxiv - Physiology 2024Quote: ... viral media was collected and passed through a 0.45 μM low-protein binding filter (Millipore) and concentrated by low-speed centrifugation (3300 g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... beads were incubated directly with 4X protein loading buffer (Li-Cor) with 2-Mercaptoethanol (Sigma). For digital PCR ...
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bioRxiv - Neuroscience 2023Quote: ... The fractions were then pooled based on protein concentration and Amicon centrifugal filters (EMD Millipore) were used to concentrate the samples ...
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bioRxiv - Microbiology 2023Quote: ... and pellets were diluted in 1× BugBuster® (10× Protein Extraction Reagent, Novagen® Merck), 50 mM NaH2PO4 pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and protein was eluted with a cocktail of 100 ug/ml RNase A (Sigma-Aldrich) and 0.1 units/ µL RNase H (Epicenter) ...
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bioRxiv - Cancer Biology 2023Quote: ... All the proteins were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Millipore Corporation, UFC901024).
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bioRxiv - Neuroscience 2023Quote: ... We used primary antibodies for astrocytic marker glial fibrillary acidic protein (GFAP) (chicken, Millipore, AB5541). To quantify relative intensity of staining for GFAP we measured the average signal intensity in the region of interest and normalized it to that of the WT littermate mouse ...
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bioRxiv - Biochemistry 2023Quote: ... Polyubiquitinated proteins were visualized by immunoblotting for ubiquitin (Millipore Sigma 05-994, clone P4D1-A11) and actin was used as loading control (Millipore Sigma MAB1501R ...
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bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were detected by Western blotting using the following primary antibodies against: Flag (M2, Sigma), ERK1/2 (3A7 ...
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bioRxiv - Neuroscience 2023Quote: ... Protein bands were detected with ECL Prime Western blotting Amersham (Sigma Aldrich, cat. no GERPN2232) detection reagent and images acquired with STELLA imaging system ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted by incubation with 25 µL of 100 µg/ml 3xFLAG peptide (Sigma) in 0.05% RapiGest (Waters Corp ...
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bioRxiv - Biophysics 2024Quote: ... the protein was further purified by binding to ANTI-FLAG M2 Magnetic Beads (Sigma Aldrich) and eluted with 3× FLAG Peptide (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 mg of nuclear protein was concentrated in 500ul using a Microcon centrifugal filter (Millipore) and then applied to a Superose 6 size exclusion column (10/300 GL GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoblotting loading samples were prepared after adjusting the protein concentration using 5X SDS (Sigma-Aldrich) and denatured by boiling ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Cancer Biology 2023Quote: Equal amounts of protein or EVs were mixed with reduced (containing 5% β-mercaptoethanol, Sigma) or non-reduced 4 x Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Plant Biology 2023Quote: ... The AGO1 protein was detected by Western blot using a Flag-HRP antibody (A8592, Sigma).
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bioRxiv - Cell Biology 2023Quote: Protein lysates were resolved in SDS-PAGE gels and transferred to PVDF (Millipore, 0.2 µm) membrane ...
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bioRxiv - Microbiology 2023Quote: ... The sample was then added with 75 μl pre-washed Protein A Sepharose (Sigma, #P6649) and incubated at 4 °C for 30 min with rotation ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies against the following proteins were purchased from the indicated vendors: MAP2 (EMD Millipore AB5622); SLC17A6 (Alomone Labs AGC-036-GP) ...
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bioRxiv - Microbiology 2024Quote: ... protein expression was induced by adding 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma) and 1 mg/mL riboflavin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Coding sequences for M and Enn proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site ...