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Citations for Millipore Sigma :
7051 - 7100 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: Proteins were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, IPVH00010) for immunoblotting ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins solubilized into serum-containing media were pulled down by using heparin-sepharose beads (Sigma). Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5) ...
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bioRxiv - Molecular Biology 2024Quote: Protein samples were separated on Bis-Tris gels and transferred to a PVDF membrane (Millipore). For immunoblots showing multiple proteins with similar molecular weight ...
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bioRxiv - Cancer Biology 2024Quote: ... was used to probe target proteins with the BCIP®/NBT Liquid Substrate (Sigma-Aldrich). ImageJ software (National Institutes of Health ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing pure polyhistidine-tagged proteins were pooled and concentrated by centrifugation filtration (EMD Millipore; using appropriate MWCO ...
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bioRxiv - Biochemistry 2024Quote: ... The disulfide bridges in the protein extract were reduced in 10 mM dithiothreitol (DTT, Sigma) for 1 h at 37 °C and shaking at 1000 rpm in a TC ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 30K MWCO to 50-100 µM ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions which contained full-length protein were pooled and concentrated in an Amicon (Millipore) 4 mL centrifugal concentrator 3K MWCO to 100-200 µM ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were stained for microtubule-associated protein 2 (Anti-MAP2, Rabbit; Millipore-Sigma; RRID: AB_5622). Following primary antibody incubation ...
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bioRxiv - Plant Biology 2024Quote: Yeast protein was extracted by homogenizing yeast cells with acid-washed glass beads (Sigma-Aldrich) in homogenizing medium (0.25 M sorbitol ...
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bioRxiv - Pathology 2024Quote: ... Proteins were then transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...
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bioRxiv - Cell Biology 2024Quote: ... The eluted proteins were concentrated to 1 mL with a 30K MWCO concentrator (EMD Millipore) and diluted with 1 mL buffer A ...
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bioRxiv - Neuroscience 2024Quote: ... The resolved proteins on the gel were then transferred to Immobilon-FL PVDF membranes (Millipore) at 100V for 1 hr 12 mins ...
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bioRxiv - Genetics 2024Quote: ... proteins were loaded on an 8% polyacrylamide gel and transferred onto a PVDF membrane (Millipore). Chemiluminescence was induced by Luminata Forte Western HRP Substrate (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... Eluted protein was concentrated to 2 mL by centrifugation using Amicon Ultra centrifugal filters (Millipore) with a 50-kDa molecular weight cut-off (MWCO ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Biophysics 2024Quote: ... Purified proteins were concentrated using Amicon Ultra-15 Ultracell-30K centrifugal filter units (Millipore–Sigma), then centrifuged at 100,000 rpm at 4 °C for 10min using a Beckman TLA-120.2 rotor to remove aggregates ...
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bioRxiv - Molecular Biology 2024Quote: ... whole cell protein lysates were prepared in RIPA buffer freshly supplemented with proteinase inhibitors (Millipore) and phosphatase inhibitors (Millipore) ...
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bioRxiv - Microbiology 2024Quote: ... protein expression was induced by adding 50 μM isopropyl β-D-1-thiogalactopyranoside (IPTG; Sigma) and 1 mg/mL riboflavin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: Coding sequences for M and Enn proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated with addition of Trichloroacetic acid (TCA) 100% (1/5v/v) (Sigma #T6399) and washed with 0.01M HCl/ 90% Acetone before by peptide identification by LC/MS.
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bioRxiv - Neuroscience 2024Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich 68957) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich 22790) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysates were mixed with 30 µL of Protein G Sepharose 4B (Sigma-Aldrich) and rotated at 4°C for 2 h with 2.5 µg of mouse anti-Myc antibody (Calbiochem) ...
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bioRxiv - Molecular Biology 2024Quote: ... Bait-purified prey proteins from plasma were then eluted with 5 mM biotin (Sigma-Aldrich) in ice-cold 1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... The proteins in the gel were transferred onto polyvinylidene difluoride membranes (Merck Millipore, Billerica, MA). After protein transfer ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested overnight at 37°C with mass spectrometry grade trypsin (SOLu-Trypsin, Sigma) in an enzyme:substrate ratio of 1:50 ...
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bioRxiv - Physiology 2024Quote: ... The separated proteins were transferred to an Immobilon PVDF-FL membrane (#IPFL00005, Millipore, Billerica, MA) at 100 V ...
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bioRxiv - Pathology 2024Quote: ... Separated proteins were transferred onto polyvinylidene difluoride membranes (Immobilon-P; Merck Millipore, Burlington, MA, USA). The membranes were reacted with the following primary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Target proteins were collected and concentrated using Amicon Ultra-15 10K Centrifugal filters (Sigma-Aldrich), and stored in 20 mM HEPES ...
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bioRxiv - Systems Biology 2024Quote: ... and 0.25 ml 10 X endonuclease-free BugBuster Protein Extraction Reagent (Millipore, Cat. No. 70921) was added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein-containing fractions were concentrated using a 3 kDa cutoff Amicon centrifugal filter (Sigma UFC8003) and loaded onto a Superdex 75 size exclusion column equilibrated with a storage buffer (25 mM Na2HPO4 pH 7.0 ...
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bioRxiv - Genetics 2024Quote: ... Proteins were then separated using 10% SDS-PAGE and transferred to PVDF membranes (Merck Millipore). PVDF membranes were then blocked by 5% BSA ...
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bioRxiv - Neuroscience 2024Quote: Protein samples were mixed 1:1 with 2X Lämmli sample buffer (Sigma-Aldrich, S3401-1VL) and denatured at 95°C with shaking at 400 rpm for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Cell Biology 2020Quote: ... Serum-free DMEM was then discarded and cells were incubated in serum-free DMEM containing 120 μg/mL human HDL (Sigma-Aldrich, L1567) for 6 h ...
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bioRxiv - Immunology 2021Quote: ... fresh living donor biopsies or cells remaining following 10X cell capture for sequencing were resuspended in 90% human serum (Sigma, cat# H4522) and 10% DMSO for cryopreservation and cooled to -80°C in a Mr.Frosty (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for immunostaining: anti-CREST/anti-centromere antibodies (human, Antibodies Inc.; 1:100) anti-Tubulin (mouse monoclonal, Sigma; 1:5000). DNA was stained with 0.5 µg/ml DAPI (Serva ...
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bioRxiv - Bioengineering 2019Quote: ... human skeletal muscle samples were minced and then dissociated into a single cell slurry with Clostridium histolyticum collagenase (Sigma, 630 U/mL) and dispase (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were blocked for 30 minutes with mouse serum and immunostained with directly Cy3-conjugated anti-human HNA (Millipore, MAB1281C3, 1:100). Nuclei were counterstained using DAPI ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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bioRxiv - Bioengineering 2019Quote: ... were maintained as monolayer cultures in flasks pre-coated with 60 μg/mL solution of human placental collagen type IV (Sigma Aldrich, MO) and grown in bronchial epithelial growth medium (BEGM ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we grew PAO1-pvdSi in iron-limited CAA medium (using 100 mg ml−1 human-apo transferrin to bind iron and 20mM NaHCO3 as cofactor, both from Sigma-Aldrich, Switzerland), supplemented with various amounts of purified pyoverdine (pyoverdine concentration in μM ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for the analysis of human synovium with microscopy: rabbit polyclonal anti-CD3 (Sigma-Aldrich Cat# C7930, RRID:AB_259074), anti-CD103 (Clone EP206 ...
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bioRxiv - Bioengineering 2021Quote: ... for 12 days in SSC expansion media with the following additions: 100 ng/mL follicle stimulating hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL luteinizing hormone from human pituitary (LH ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-absorption of mouse anti cathepsin-S with 300 nanograms of active human cathepsin-S (SRP0292, Sigma-Aldrich, St. Louis, MO) did not result in detectable immunolabeling signal.
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Freshly washed erythrocytes were diluted to 1% hematocrit in 1X PBS containing 1% BSA and coincubated for an hour with antibodies against the human glycophorin AB (GYPAB) (1:400, Sigma Aldrich UK), GYPA (Clone E4 ...