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Citations for Millipore Sigma :
6651 - 6700 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... or adult control mice (n = 6) received an intra-peritoneal (IP) injection of 1.25mg/kg LPS from Escherichia coli 0111:B4 (Sigma), 0.5mg/kg murine Tumour Necrosis Factor Alpha (TNF-ɑ ...
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bioRxiv - Cell Biology 2020Quote: ... 10 % glycerol and bromophenol blue) containing 50 μM phenylmethylsulfonyl fluoride (PMSF) and 50 μM N-ethylmaleimide (all Sigma-Aldrich). For LMα3 ECM processing blots ...
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bioRxiv - Microbiology 2021Quote: ... dissolved in PBS or 0.8 mg/ml fMLP 2.5 ml (N-Formyl-Met-Leu-Phe, F3506, Lot#MKCC5355; Sigma-Aldrich), where 2 mg fMLP was dissolved in 50 μl DMSO and then in 2.5 ml PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Clozapine-N-oxide (NIDA Drug Supply Programme) was dissolved first in 5% DMSO (Dimethyl Sulfoxide, Sigma-Aldrich, Poole, UK) and then in sterile 0.9% NaCl (Henry Schein Ltd ...
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bioRxiv - Plant Biology 2020Quote: The ability of pollen to remove OH• (generated in a Fenton reaction) was analyzed in experiments with spin trap DMPO (5,5-dimethyl-1-pylloline-N-oxide) (Sigma). First H2O2 and spin trap were added to a suspension of pollen ...
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bioRxiv - Neuroscience 2022Quote: ... we stimulated cells O/N with 15 mM LGA before incubating them with 2.5 µM calcein-AM (Sigma-Aldrich) and 0.4 µg/mL propidium iodide (PI ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Surface hydrocarbons were extracted from individual eggs and larvae using 10µl of n-pentane (≥99%, HPLC grade, Sigma-Aldrich) for 2 minutes ...
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bioRxiv - Biophysics 2022Quote: ... Purified Vnucfree solubilized in 0.03% n-DDM was mixed with the lipid stock and membrane scaffold protein MSP1E3D1 (Sigma) at a specific molar ratio of enzyme ...
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bioRxiv - Cell Biology 2022Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... paraformaldehyde (using a stock solution with 0.4 g paraformaldehyde dissolved in 10 ml 0.1 M sucrose with 0.6 µl 10 N sodium hydroxide [all from Sigma-Aldrich]), 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4 ...
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bioRxiv - Microbiology 2022Quote: ... The rest of the supernatant was incubated O/N at 4°C with 8µg (per condition) of anti-NUP98 (rat, N1038 – Sigma). 62.5µl of A/G beads per condition were added and incubated for 2h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The total membranes were solubilized with a buffer containing 1×PBS (pH 7.4) and 0.5% N-lauroylsarcosine sodium (Sigma-Aldrich) for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... but without the use of iodoacetamide for cysteine alkylation due to the treatment of 50 mM N-ethylmaleimide (Sigma) during the sample immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... live hippocampal cultures were incubated with an N-terminal APP antibody (mouse anti-APP 22C11, 1:500; EMD Millipore) diluted in conditioned neuronal media for 15 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 250 mM xylose or 225 mM N-acetylglucosamine) were applied to a TLC Silica gel 60 F254 (Sigma-Aldrich).
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bioRxiv - Microbiology 2024Quote: ... 22 mm × 22 mm #1.5 borosilicate coverslips (DOT Scientific) were placed in a Wash-N-Dry coverslip rack (Sigma) and submerged in ∼80 mL of 1 M NaOH in a 100-mL glass beaker ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were collected and raised at either 28.5 or 24°C in N- Phenylthiourea (PTU, Sigma Aldrich, Cat# P7629)/Volvic source water (0.003% final ...
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bioRxiv - Cell Biology 2024Quote: ... and extracted in 200 μl of lysis buffer (10 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% n-dodecyl-D-maltoside (DDM) (Sigma), and complete protease inhibitors (Roche) ...
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bioRxiv - Plant Biology 2023Quote: ... – N medium was prepared using 0.5x MS basal salt micronutrient solution (M0529-1L, Sigma-Aldrich, Saint Louis, MO, USA) supplemented with 1.5mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... pre-diluted in 80/20 methanol:water and 50µL 0.02 ng/µL Propionyl-L-carnitine-(N-methyl-d3) (Sigma Aldrich 52941). After vortexing for 1 min samples were retuned to −80°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cancer Biology 2024Quote: ... Larvae were maintained in E3 containing 0.2 mM N-phenylthiourea beginning 1 day post fertilization (dpf) (PTU, Sigma Aldrich) to prevent pigment formation during imaging experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... The homogenates were then lysed on ice for 30 min in nucleus lysis buffer (HEPES 50 mM, EDTA 10 mM, N lauryl sarkosyl 0.5%, sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma). DNA was sheared using a Bioruptor pico from Diagenode for 10 cycles (30 sec on ...
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bioRxiv - Neuroscience 2024Quote: ... genetic A and B mixture, LGAB) and hydrochloric acid (1.0 N solution, material number: H9892, HCl) were purchased from Sigma–Aldrich (St ...
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bioRxiv - Molecular Biology 2024Quote: Synechocystis cultures (50 mL) grown under different conditions were supplemented with 10 mM N-ethyl-maleimide (NEM, Sigma-Aldrich) and further cultivated for 1 h to block free cysteine residues ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Immunology 2024Quote: ... Ifngr1flox/flox male mice (n=4/group) were daily administrated intraperitoneally with tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) solution (20 mg/mL stock solution dissolved in corn oil (Aladdin ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The reconstituted visual pigments were extracted using 1% n-dodecyl b–D-maltoside (DDM) (Sigma-Aldrich, Saint Louis, MO) in 10 ml of extraction buffer (pH 6.7 ...
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bioRxiv - Bioengineering 2022Quote: ... PLL labeled with fluorescein 5-isothiocyanate (PLLFITC) and 5(6)-Carboxytetramethylrhodamine N-succinimidyl ester (Rhod) were purchased from Sigma.
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bioRxiv - Microbiology 2023Quote: ... Pelleted cells were resuspended in 1X PBS with 100 mM N-ethylmaleimide (NEM) (Cat#128-53-0, Millipore Sigma) for 15 minutes at room temperature27 ...
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bioRxiv - Physiology 2023Quote: ... Second derivatization was with 35 µL N,O-Bis(trimethylsilyl)trifluoroacetamide (15238- 10x.1ml, Sigma-Aldrich, St. Louis MO) on thermo-shaker at 37°C for 40 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by addition of 4 μl (× n samples) of monoclonal anti-Flag M2 antibodies produced in mouse (Sigma-Aldrich). The suspension was moved to 4 °C and rotated for >3 h to conjugate antibodies to magnetic beads ...
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bioRxiv - Biochemistry 2023Quote: Purified LceB was dialyzed O/N at 4 °C with the addition of 1:200 mg/mg LceB:thrombin (Sigma) to remove the N-terminal 6His-tag ...
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bioRxiv - Microbiology 2023Quote: ... 1x106 ATCC46645-H2A:mRFP and ATCC46645-H2A:mRFP Bir1OE-N conidia were inoculated in 100 mL of RPMI + 0.5 ug/mL Voriconazole (Sigma-Aldrich) and incubated at 50 RPM for 6 hrs at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Neuroblastoma cell line SK-N-AS was maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The supernatants were then mixed with freshly prepared Griess solution consisting of 0.1% N-1-naphthylethylenediamine dihydrochloride (Sigma, #N9125) and 1% sulfanilamide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: PAW-induced outer membrane permeability was measured based on fluorescent dye N-phenyl-1-naphthylamine (NPN; Sigma-Aldrich, Australia) uptake [22] ...
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bioRxiv - Plant Biology 2023Quote: ... Spores were grown on solid 0.5× Gamborg B-5 media for 5 days and dispensed in 6-well plates containing 4 mL of liquid 0.5× Gamborg B-5 plus supplements: 0.1% N-Z amino A (Sigma #C7290) 0.03% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... and microforged glass capillaries (25 to 30µm inner diameter) filled with mineral oil (ref. n°M5904, Merck/Sigma-Alrich). 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx. ...
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Post-stroke rapamycin treatment improves post-recanalization cerebral blood flow and outcome in ratsbioRxiv - Neuroscience 2023Quote: Animals were randomized with the sealed envelope method to receive either rapamycin (Sigma Aldrich, 250 μg/kg, n=9) or vehicle (<5% ethanol in saline ...
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bioRxiv - Biophysics 2023Quote: ... a solution of 50 μM αS and 1 μM N-protein in 20 mM Tris buffer (Sigma-Aldrich, UK), pH = 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3 mM aniline dimer solution was prepared by dissolving 5.5 mg of N-phenyl-p-phenylenediamine (Sigma-Aldrich, 241393) in 10 mL of Tyrode’s solution ~20 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... consisting of PBS supplemented with 1mM of the amino-reactive MA-NHS (Methacrylic Acid N-Hydroxysuccinimide Ester, Sigma-Aldrich). Specimens were incubated for 1-1.5 hours on gentle rocking at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... The DNA corresponding to the fxn gene was amplified using KOD hot start master mix (Cat. N° 71842 Millipore) and FwDdFXNOE and RevDdFXNOE as primers (Table 1) ...
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bioRxiv - Genetics 2023Quote: ... 10-200 mg tissue was digested in 1 ml 3 N hydrochloric acid (Fisher)/10% trichloroacetic acid (Millipore Sigma) at 65 °C for two days ...
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bioRxiv - Biochemistry 2023Quote: ... the protein (with N-terminal His6 tag) was concentrated using a centrifugal filter (Amicon Ultra, MWCO 30 kDa, Sigma) to 8.11 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used for immunoblotting included anti-SARS-CoV-2 N (SinoBiological 40143-R001) and GAPDH (Millipore-Sigma G8795) monoclonal antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and lysed in NP-40 buffer supplemented with 10 mM N-ethylmaleimide (Sigma, catalog no.: E3876). Flag-immunoprecipitations were performed as described previously ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended the cell pellet in 150 μl ice cold 650ug/mL N-Ethylmaleimide (NEM; Sigma-Aldrich, St. Louis, MO) in PBS solution ...