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Citations for Millipore Sigma :
6451 - 6500 of 10000+ citations for QuantiChrom β N Acetylglucosaminidase Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... Half of the media was removed and replaced after 48h supplemented and with 4μM cytosine 1-β-d-arabinofuranoside (Ara-C; Sigma). Neurons were fed by replacing half the volume of spent media with fresh media without serum or Ara-C every week thereafter.
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bioRxiv - Biochemistry 2021Quote: ... 10 mM β-mercaptoethanol and 5% glycerol was mixed gently with 20 µL of 1 M dimethylamine-borane complex (Sigma) per 1 mL of protein solution ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Biophysics 2019Quote: ... glucose oxidase Type VII (product No. G2133), β-mercaptoethanol (product No. 63689), and polyvinylalcohol (PVA, 25,000 MW, prod. No. 184632) were purchased from Sigma Chem ...
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bioRxiv - Cell Biology 2020Quote: ... filled with STORM buffer (25 mM β-mercaptoethylamine [Sigma; 30070], 0.5 mg/ml glucose oxidase [Sigma; G2133], 67 μg/ml catalase [Sigma; C40] ...
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bioRxiv - Bioengineering 2020Quote: ... the culture medium was supplemented with 4 mM β-glycerophosphate and 50 μg/ml ascorbic acid (both from Sigma-Aldrich), starting from day 3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... BglC-CtDoc and BglC-AcDoc was measured using the synthetic substrate p-nitrophenyl-β-D-glucopyranoside (pNPG; Sigma-Aldrich, USA) following the previously described protocol4 with some modifications ...
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bioRxiv - Synthetic Biology 2021Quote: ... Induction of the expression systems was performed using varying concentrations of isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich, I6758).
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bioRxiv - Plant Biology 2021Quote: ... The reaction was started by adding 25 µl of 20 mM 4-Methylumbelliferyl-β-D-glucuronide hydrate (MUG; Sigma-Aldrich) substrate to the samples and a 20 µl aliquot was immediately added to 180 µl stop buffer (0.2 M Na2CO3 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was normalized for all tissues with RIPA buffer and 2x SDS sample buffer containing β-mercaptoethanol (Sigma-Aldrich) was added ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mM imidazole and 0.5 mM β-mercaptoethanol) and resuspended in 300 µl of hypotonic buffer containing 0.5% NP-40 (Sigma-Aldrich) and 200 U Benzonase (Novagen ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the fixed cells were incubated in the staining solution [0.2 mM fluorescein di- β -D-galactopyranoside (FDG, Sigma-Aldrich F2756), 0.2 M citric acid ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies used were chicken anti-GFP (1:10,000, Aves GFP-1010) and mouse anti-β-tubulin (1:5000, Sigma T4026).
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bioRxiv - Biochemistry 2021Quote: Antibodies used for Western Blot include mouse monoclonal anti β-actin (clone AC-15, Sigma, St. Louis, MO, United States) at 0.13 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Partial depletion of cholesterol was achieved by incubating the cells in 4 mM methyl-β-cyclodextrin (MβCD, Sigma Aldrich: 332615) at 37°C for 30 min or in 60 μg/ml saponin (Nacalai Tesque ...
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bioRxiv - Microbiology 2021Quote: ... The enzyme reaction was started by the addition of 0.16 ml of 6 mM p- nitrophenyl β-D-glucuronide (Sigma) to the broken cells and stopped by the addition of 0.4 ml of 1.0 M NaCO3 ...
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bioRxiv - Immunology 2021Quote: Liver was collected and finely minced in digestion media (RPMI, 1% penicillin-streptomycin, 1mM sodium pyruvate, 0.1% β-mercaptoethanol, 25mM HEPES, 150μg/ml DNase I [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2021Quote: ... and the expression was induced by the addition of 1 mM isopropyl-β-D-1-thiogalactopyranoside (IPTG) (Sigma-Aldrich # I6758). After the addition of IPTG ...
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bioRxiv - Cell Biology 2022Quote: ... The mouse monoclonal anti- FLAG (catalog #: F1804) and anti-β-actin (catalog #: A1978) antibodies came from Sigma (St. Louis, MO). The mouse monoclonal anti-Hsp47 antibody (catalog # ...
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bioRxiv - Microbiology 2022Quote: ... samples were washed with 1 ml PBS buffer three times and vacuum-infiltrated for 20 min with 0.1 mg/ml of primary mouse anti-1,3-β-glucan antibody (Biosupplies; Sydney, Australia) for β-1,3-glucan labelling or 0.1 mg/ml mouse MOPC-104E primary antibody (Sigma-Aldrich) for α-1,3-glucan labelling ...
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bioRxiv - Neuroscience 2023Quote: ... Afterwards the samples were incubated with primary antibodies (anti-β-tubulin III produced in rabbit (Sigma, Ireland, T2200, 1:400) and anti-GFAP produced in mouse (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Synthetic Biology 2023Quote: ... 30 μL were diluted into 2 mL LB with 300 μg/mL 3,4-cyclohexenoesculetin-β-D-galactopyranoside (S-gal) (Sigma), 500 μg/mL ferric ammonium citrate (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 mM β-mercaptoethanol) followed by sonication using a soniprep 150 (MSE) probe sonicator or in RIPA buffer (Sigma-Aldrich), R0278 supplemented with 1× SIGMAFAST protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2022Quote: ... 500 mM NaCl and 6 mM β-mercaptoethanol) containing 1 mM phenylmethylsulfonyl fluoride (PMSF) and Protease inhibitor cocktail (Sigma-Aldrich) (20 mg/g of cell pellet) ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to a final concentration of 1% and incubated for 5 minutes at 37°C shaking at 800 rpm and the reaction quenched with 60µl β-mercaptoethanol (Sigma). DMS modified RNA was purified using RNA Clean and Concentrator-5 kit and eluted in 10 ul water ...
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bioRxiv - Bioengineering 2022Quote: ... medium was switched to osteogenic differentiation medium (osteogenic control medium with osteogenic supplements 10 mM β-glycerophosphate (G9422, Sigma-Aldrich), 50 µg/ml ascorbic acid-2-phosphate (A8960 ...
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bioRxiv - Molecular Biology 2024Quote: The following monoclonal and polyclonal antibodies were used: anti-β-actin (WB: 1 µg/ml, AC-15/A1978, Sigma-Aldrich), anti-Miro1/2 (WB ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µM β-mercaptoethanol and 1% PenStrep) supplemented fresh with 10 µM SB431542 (Milteny Biotec) and 0.1 µM LDN-193189 (Sigma). 3N neural differentiation medium with SB and LDN was replaced daily for 10 days ...
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bioRxiv - Cancer Biology 2023Quote: ... staining solution (0.3 mg/ml of 5-bromo4-chloro-3indolyl β-D-galactoside, X-Gal Fermentas R0401, 40 mM citric acid, Sigma C-0759 ...
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bioRxiv - Immunology 2023Quote: ... T cell response was determined by cleavage of the chromogenic LacZ substrate chlorophenol red-β-D-galactopyranoside (CPRG, Millipore Sigma) by TCR-induced LacZ and measure by spectrophotometry as presented by absorbance at 595 nm (A595 ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Microbiology 2023Quote: ... Pretomanid (Pa) (RTI) was dissolved in deionized water containing cyclodextrin micelle (CM-2) formulation,10% hydroxypropyl-β-cyclodextrin (HPCD) (Sigma) and 10% lecithin (ICN Pharmaceuticals Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... Expression was controlled by T7 promoter and induced by 1 mM Isopropyl-β-D-thiogalactopyranosid (IPTG) (Sigma Aldrich, Steinheim, Germany) at an OD600 of 0.8-1.0 ...
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bioRxiv - Immunology 2024Quote: ... Antibody for β-actin (Catalog no. A2228) and human α-thrombin (Catalog no. T6884-100N) was purchased from Sigma-Aldrich. All other reagents were bought from Fisher-Scientific except where noted.
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bioRxiv - Cell Biology 2024Quote: ... 0.5 mM/L β-mercaptoethanol and 10 mM nicotinamide and 10 nM exendin-4 (Cat no: E7144 Sigma-Aldrich, USA) until day 28 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was mixed with about 10 time molar excess of guanosine 5′- [β,γ-imido]triphosphate (GMMPPNP, SIGMA G0635) or guanosine 5’-triphosphate (GTP ...
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bioRxiv - Biophysics 2024Quote: ... Gene expression was induced at OD600 ∼0.8 by addition of either 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG; Carl Roth GmbH) or 0.2 µM anhydrotetracycline (Sigma). Cells were grown overnight at 22°C ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were re-probed with the HRP-conjugated primary anti-β-actin antibody for 20 min (Millipore-Sigma, 1:50,000), and the β-actin immunoreactivity was visualized.
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for 1h at room temperature in blocking solution (5% non-fat dried milk and 0.1% Tween 20 in PBS) and then overnight at 4°C with the primary antibody (mouse anti-β-ACTIN, 1:30,000, Sigma; rabbit anti-AKT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and CasX2 expression induced with the addition of 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG, EMD Millipore, Burlington, MA). After 16 hours at 16°C ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The primary antibodies used in immunofluorescence (IF) were rabbit anti-LvIRF antibody (Genecreate) and mouse anti-β-actin antibody (Sigma). The slides were washed with PBS six times and then incubated with diluted second antibodies of anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2023Quote: 5 x 107 cells for each Jurkat cell line were incubated in the presence of 10 μM 5,6-Dichlorobenzimidazole 1-β-D-ribofuranoside (DRB, Sigma) or DMSO vehicle control for 2 h under normal culture conditions ...
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PQS and Pyochelin in Pseudomonas aeruginosa Share Inner Membrane Transporters to Mediate Iron UptakebioRxiv - Microbiology 2023Quote: ... A total of 100 µL of 4 mg/mL 2- nitrophenyl β-D-galactopyranoside (ONPG) (Sigma, St. Louis, MO, USA) was added to the cells ...
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bioRxiv - Developmental Biology 2023Quote: ... pregnant mice were administered vehicle (0.1875% 2-hydroxy-β-cyclodextrin) or 50 µg/mL CORT (Sigma-Aldrich, St. Louis, MO) in distilled drinking water (Figure 1A).
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bioRxiv - Cell Biology 2023Quote: ... α/β-tubulin (final concentration 10 µM) was added to a solution containing MgCl2 and glutamate (Sigma Aldrich, 49621-250G) with a final concentration of 0.88 µM and 0.8 mM ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were developed by chemiluminescence following incubation with secondary HRP-conjugated antibodies (1:2000) and an HRP-conjugated anti-β-actin antibody (1:40.000, Sigma) was used as loading control ...
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bioRxiv - Microbiology 2023Quote: ... and pretreated 1 h at 37°C with either 5 mM Methyl-β-cyclodextrin (MBCD) (Sigma Aldrich, stock in ddH2O) in OptiMEM medium buffered with 25 mM HEPES (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The LRP1 α-chain (515 kDa) and β-chain (85 kDa) were detected using antibodies 8G1 (2μg/mL) (Merck-Millipore) and 5A6 (2μg/mL ...