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Citations for Millipore Sigma :
6801 - 6850 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cell lysates were subjected to SDS-PAGE and analyzed by Western blotting using an anti-HA primary antibody (rabbit; Millipore, #15-902R), an HRP-conjugated goat anti-rabbit secondary antibody (PerkinElmer ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies for immunostaining include rabbit anti-human/mouse PKD-1 (4502371) and mouse anti-mouse α-smooth muscle actin(A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Cancer Biology 2021Quote: ... To the remaining cell lysate was added anti-H3K4me3 antibody-coated Dynabeads M-280 Sheep anti-Rabbit IgG (5 μg antibody per sample, Millipore, 04-745), followed by rotation at 4°C overnight for immunoprecipitation ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated in rabbit anti-Iba1 (1:1000, Wako, Cat.#019-19741) and chicken anti-GFAP antibody (1:2000, Sigma, Cat#AB5541) dissolved in PBST at 4 °C for 24 hours ...
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bioRxiv - Cell Biology 2022Quote: 1 mg of Triton-soluble samples prepared as described in (9) were incubated with 4 μl of Cx43 antibody (Sigma C6219, rabbit) and subsequently immunoprecipitated ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with HRP-conjugated secondary antibody (1:10,000; anti-rabbit, Sigma-Aldrich A0545 or anti-mouse, Sigma-Aldrich A0168) in TBST with 1% milk ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were added for 48 hours at 4°C and included the mouse monoclonal antibody described above (31G10) (5) as well as commercially available cell type-specific markers including: choline acetyltransferase (ChAT; AB143; rabbit polyclonal; Millipore, Burlington, MA); COUP TF1-interacting protein 2 (CTIP2 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The Duolink procedure was conducted according to the manufacturer’s instructions with the Duolink® In Situ Red Starter Kit Mouse/Rabbit (Duolink®, Sigma□Aldrich), with secondary antibodies contained in the kit ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed five times with PBS and incubated 2 h at RT in the dark with FITC-coupled α-rabbit antibodies (1:200 in BSA-PBS, Sigma Aldrich). After washing and drying ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4 °C with primary antibodies (GSX2: rabbit 1:200, Merck-Millipore ABN162; PAX6 mouse 1:80, DSHB) diluted in solution containing 2.5% NGS and 0.02% Triton X-100 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with one or more of the following primary antibodies (rabbit anti-TH, 1:2000, AB152, EMD Millipore; guinea pig anti-VGLUT2, AB2251, EMD Millipore; chicken anti-GFP ...
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bioRxiv - Neuroscience 2020Quote: ... of fixed slice cultures were prepared and stained with antibodies against Iba1 (rabbit, 1:1000; Fujifilm Wako #019-19741) and GFAP (mouse, 1:1000; Sigma-Aldrich #G3893). Slices were incubated in appropriate Alexa Fluor® dye-conjugated secondary antibodies (donkey ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with the following primary antibodies at 4°C overnight: rabbit anti-KCC2 (1:1000, Cat#07-432, Millipore, RRID: AB_310611) and goat anti-ChAT (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: Lysates and elutions were resolved by SDS-PAGE and analyzed by western blot in TBST (Tris-buffered saline, 0.2% Tween 20) using the following primary antibodies: rabbit α-FLAG (Sigma, 1:2500); Strep-HRP (Millipore ...
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bioRxiv - Immunology 2020Quote: ... the primary antibody was discarded and the preparations probed with a secondary antibody (anti-rabbit, conjugated to Fluorescein isothiocyanate, FITC (Sigma-Aldrich, Germany), prepared at a dilution of 1:10000 in PBST for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were fixed with 4% PFA and immunostained with mouse anti-HA (Covance, 1:1000) or rabbit anti-Flag (Sigma, 1:1000), followed by incubation with AlexFluor-647-conjugated anti-human Fc γ and species-specific AlexaFluor-488- or -546-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices with Cav2-Cre injection at MPC and lENT were immunostained by Rabbit anti-Cre antibody (Millipore Sigma, Cat#69050-3) and Donkey anti-Rabbit secondary antibody ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were subjected to blocking in 5% milk in TBST and incubated overnight with respective primary antibodies - rabbit anti-HA (1:1500, Cat. No. H6908, Sigma-Aldrich, USA), rabbit anti-c-fos (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed with PBS and incubated with secondary antibodies: goat anti-rabbit Alexa Fluor 488 1:500 AP132JA4 (Millipore, Etobicoke, CAN) and goat anti-mouse Alexa 546 1:500 AP192SA6 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... sections were stained with primary (1:1000, guinea pig anti-NeuN, Millipore #ABN90P; 1:1000, mouse anti-NeuN, Millipore #MAB377; 1:300, rabbit anti-PCP4, Sigma Aldrich #HPA005792) and secondary antibodies (Alexa Fluor 647 goat anti-guinea pig IgG ...
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bioRxiv - Neuroscience 2019Quote: ... c-Fos counts were blindly performed immunohistochemically for staining of cocaine (10mg/kg; i.p.)-induced rabbit anti-c-Fos (1:10K, EMD Millipore, MA, USA).
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bioRxiv - Neuroscience 2019Quote: ... After incubating overnight in blocking solution containing a primary antibody (polyclonal rabbit anti-tyrosine hydroxylase; 1:1000 dilution; Millipore; catalog no. 657012), sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed in PBS and PBS-TX followed by incubation with the relevant primary antibody (rabbit anti choleratoxin (Sigma-Aldrich UK) or rabbit anti-Mcherry (1:2000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were incubated with guinea pig antibody to D1 receptors (1:400; Alomone, Jerusalem, Israel) and rabbit antibody to D2 receptors (1:500; Millipore, Burlington, MA), washed ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... Blots were incubated with appropriate secondary antibodies (Anti-Rabbit IgG-Peroxidase Sigma-Aldrich A0545 and Anti-Mouse IgG-Peroxidase Sigma-Aldrich A9044) and imaged using GE Amersham Imager 600.
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bioRxiv - Pathology 2021Quote: ... Rabbit polyclonal anti-calnexin (CNX) antibody (Cat# SAB4503258, RRID:AB_10746486) and all other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Developmental Biology 2021Quote: ... To determine the Cdc42-borg4 and Borg4-septin7 interactions the cells were then incubated with primary antibody mixtures Cdc42 (Millipore rabbit polyclonal) and Borg4(Santa Cruz ...
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bioRxiv - Pathology 2021Quote: ... Primaries were prepared in 10% ngs and applied as combinations of either: anti-Aβ1-40 rabbit polyclonal (Millipore Sigma #AB5074P, 1:200) and anti-Aβ1-42 12F4 mouse monoclonal (Biolegend #805502 ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were permeabilized and non-specific binding sites were blocked before incubation with rabbit anti-TH antibody (1:1000, AB152, Millipore Sigma, USA) for 48h ...
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bioRxiv - Cell Biology 2019Quote: PLA was performed according to the manufacturer’s instructions using the Duolink In Situ Orange Kit Goat/Rabbit (Sigma Aldrich, Cat. No. DUO92106). Immunofluorescence was performed as previously described (Arno et al. ...
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bioRxiv - Genomics 2021Quote: ... Cells were immunostained with rabbit antibody against Histone H3 (phospho S10) (1: 5000) to identify mitotic cells and mouse anti-Tubulin (Sigma; 1:500) to assay cell viability and mitotic stage as previously described55 ...
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bioRxiv - Cell Biology 2020Quote: ... The membrane was probed against the endogenous proteins by incubation with primary Rabbit anti-myosin VI (1:500, Atlas-Sigma HPA0354863-100UL) or Mouse anti-RNAPII phospho Ser5 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... Duolink® In Situ PLA Probe Anti-Rabbit MINUS and Anti-Mouse PLUS and Duolink® In Situ Detection Reagents (Sigma-Aldrich) were used ...
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bioRxiv - Neuroscience 2020Quote: Immunostaining for ChAT or TH was performed using the following antibodies: rabbit anti-TH 1:200 (Pel-Freez cat # P4010-0) or goat anti-ChAT 1:25 (Millipore cat # AB144P). Sections were fixed in an acetone/methanol mixture (1:1 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated at 4°C for 48 hr in blocking buffer containing a rabbit anti-c-Fos antibody (1:3000, ABE457, Millipore, Bellerica, MA). After washes in TNT (0.1 M Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies used were rabbit polyclonal against Arl13b (kindly provided by Dr. Tamara Caspary, Emory University) and a mouse monoclonal against acetylated α-tubulin (Sigma-Aldrich). Cells were stained with secondary antibodies along with DAPI diluted in dilution buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein detection was achieved using the following antibodies: anti-Toddler (rabbit, 1:500, provided by the Reversade Lab11) and anti-tubulin (mouse, 1:10000, Sigma-Aldrich T6074).
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: ... Antibody solution consisted of blocking solution with rabbit anti-Iba1-635 (Wako, cat. # 013-26471) at 1:400 and DAPI (Sigma, cat. # D9542) at 10 µg/mL.
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bioRxiv - Microbiology 2021Quote: ... (80) and α-PilC (1:5000 dilution) (81) antibodies were used together with horseradish-conjugated goat anti-rabbit immunoglobulin G (Sigma-Aldrich) or anti-mouse sheep IgG antibody (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: ... and incubated with Cy-3-conjugated goat anti-rabbit IgG as the secondary antibody (1:500, Cat # AP132C, Millipore Sigma, Burlington, MA) at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight followed by goat anti-rabbit secondary antibody conjugated with Cy3 for 1 hr at room temperature (1:500, Millipore, Cat. #AP132C). Since the purpose of this staining was to evaluate the presence of extracellular HMGB1 in the tendon ...
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bioRxiv - Cell Biology 2021Quote: ... tissue sections were washed 3 times with PBS and incubated at room temperature for 2 hrs with Cy3-conjugated goat anti-rabbit IgG antibody (1:500, Millipore, Cat. #AP132C).
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bioRxiv - Genetics 2020Quote: ... using 1:1000 rabbit anti-Bestrophin1 antibody (LAgen Laboratories; Cat# 016-Best1-01) and 1:1000 mouse anti-Actin antibody (Millipore; Cat# MAB1501) as primary antibodies.
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bioRxiv - Neuroscience 2021Quote: ... Cells were permeabilized for 10 minutes in 0.5% Triton X in PBS and incubated for 48 hours at 4°C in rabbit anti-NG2 (AB5320, Merck Millipore; 1:500) and rat anti-MBP (MCA409 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated in PBS-T-milk for 1 h using 1:10000 dilutions of anti-rabbit or anti-mouse HRP-linked secondary antibodies (A0545 or A9044 Sigma antibodies, respectively) as required ...
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...