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Citations for Millipore Sigma :
6351 - 6400 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... before staining in Carmine Alum solution overnight at room temperature (0.2% (w/v) carmine and 10 mM aluminium potassium sulphate (Sigma)) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Conidia from the lesions on the sugar beet leaves were liberated into 30 μL sterile water (0.02% ampicillin) with a pipette tip and transferred to water agar plates (1.5% w/v agar (Sigma-Aldrich) and 0.02% ampicillin) ...
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bioRxiv - Plant Biology 2020Quote: ... Destained material was hydrated with 0.07 M phosphate buffer (pH 9.0) for 30 min and then incubated for 15 min in 0.1% (w/v) aniline blue (Sigma-Aldrich) and 0.005% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then washed 3 times with TBS containing 1% BSA and 0.1% w/v sodium azide (Sigma Aldrich). After staining ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were allowed to adhere to slides coated with 10% Poly-L-lysine solution (0.1% w/v in H2O) (P8920, Sigma) for 1-3h at 37°C in a HERAcell 150i or 160i 5% CO2 incubator (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... 375 μL of methanol as extraction solvent and 25 μL of a 10 % (w/v) solution of canthaxanthin (Sigma) in chloroform as internal control ...
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bioRxiv - Microbiology 2021Quote: ... Microbial cells were detached from soil particles by mixing with sterile 0.2% (w/v) tetrasodium-pyrophosphate decahydrate solution (pH 7.5, Sigma-Aldrich), and then purified by sucrose gradient solution centrifugation as described by (61) ...
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bioRxiv - Synthetic Biology 2020Quote: ... the virus was removed and the cultures overlaid with a viscous solution of 1% (w/v) carboxymethylcellulose (Sigma #C4888) in DMEM-F12 (8% FBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... Seeds were then sown on solid sterile ½ strength MS medium [0.215% (w/v) Murashige‐Skoog (MS) salts (Sigma‐Aldrich), 0.8% agar (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... Culture was washed three times in PBS (300 g, 5 minutes) and parasites isolated from erythrocytes using 0.03% (w/v) saponin (Sigma) on PBS containing protease inhibitors ...
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bioRxiv - Neuroscience 2019Quote: ... were briefly anesthetized with isoflurane and retro-orbitally injected with 50μL of 2.5% w/v of Texas-red conjugated dextran (40 kDa; Sigma-Aldrich), then head-fixed upon a spherical treadmill ...
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bioRxiv - Physiology 2020Quote: ... Slides were then washed twice in warm water for 5 min and stained for 1h at RT with Picro-Sirius Red solution (0.1% (m/v) Sirius Red (Sigma) in saturated aqueous solution of Picric Acid (LabChem)) ...
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bioRxiv - Microbiology 2019Quote: ... and were maintained in Roswell Park Memorial Institute (RPMI) medium supplemented with 0.15% (w/v) sodium bicarbonate (Sigma-Aldrich), 0.1 mM non-essential amino acids ...
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bioRxiv - Microbiology 2019Quote: ... Adherent cells were stained with 200 μL of the crystal violet solution (0.1%, w/v) (Sigma–Aldrich, Munich, Germany) for 30 min at RT ...
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bioRxiv - Genomics 2021Quote: ... with DAPI-containing antifade mounting media (0.3 μg/ml DAPI (SigmaAldrich)/10% w/v Mowiol 4-88(EMD Millipore)/1% w/v DABCO (Sigma-Aldrich)/25% glycerol/0.1 M Tris ...
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bioRxiv - Microbiology 2021Quote: ... and separated from uninfected erythrocytes on a Histodenz cushion made from 48% of a Histodenz stock (27.6% [w/v] Histodenz [Sigma/Alere Technologies ...
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bioRxiv - Genomics 2021Quote: ... 10 mM TAPS pH 8.5 with addition of 10% (w/v) 1,6-hexanediol (Sigma-Aldrich cat. no. 240117-50G) or 10% (v/v ...
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bioRxiv - Genomics 2021Quote: ... were captured using 40 μl of protein-A or protein-G Dynabeads (Invitrogen; pre-washed with 0.1% BSA [Fraction V, Sigma), by 4 hrs incubation time at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and then fixed with 4% paraformaldehyde (PFA) for 30-45 minutes and stained with 0.5% (w/v) crystal violet (Sigma) for 20 minutes before being rinsed with tap water and dried at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were fixed overnight with 95% ethanol and after fixation were submerged in Alcian Blue solution (0.03% w/v Alcian Blue (Sigma), 80% ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... at which time 15 larvae were transferred to freshly prepared 1% (w/v) DSS (SIGMA, D8906-10G, MW>500,000) in E3 for a single overnight treatment ...
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bioRxiv - Systems Biology 2021Quote: ... All assays were terminated by fixing and permeabilizing the cells with 20μl of a solution containing 0.5% (w/v) formaldehyde (Sigma-Aldrich), 0.05% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... counted and centrifuged and washed twice in flow buffer −0.5% mass/volume (w/v) bovine serum albumin (BSA) in PBS (both from Sigma). A total of 50 000 cells from each sample were transferred into a sterile polystyrene test tube (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... before staining with Carmine Alum solution at room temperature overnight (0.2% (w/v) carmine and 10 mM aluminium potassium sulphate (Sigma)) ...
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bioRxiv - Cell Biology 2020Quote: ... 7.5 % 10 % acrylamide/ bis-acrylamide; electrophoresis buffer: 25 mM Tris, 250 mM glycine, 0.1 % w/v SDS, pH 8.5. All Sigma-Aldrich) and transferred to a nitrocellulose membrane using a Biorad TurboBlot system (BioRad ...
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bioRxiv - Immunology 2020Quote: ... The plates were incubated sequentially with 500 ng/mL previously described mouse anti-ZIKV mAb ZV-165 and horseradish-peroxidase (HRP)-conjugated goat anti-mouse IgG in PBS supplemented with 0.1% (w/v) saponin (Sigma) and 0.1% bovine serum albumin (BSA) ...
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bioRxiv - Biophysics 2021Quote: Human serum albumin (fraction V) 98% and 5,5’-dithiobis-2-nitrobenzoic acid (DTNB) were purchased from Sigma Aldrich (USA) and used without any prior purification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and permeabilized for 10 min at room temperature in the same buffer containing 0.1% TritonX-100 (W/V, Sigma) and 1 μg/ml Hoechst DNA staining (Thermo Fisher ...
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bioRxiv - Bioengineering 2021Quote: ... and 1H-NMR spectra were recorded on a Varian Unity-300 (300MHz) NMR spectrometer (Varian Inc.) using 3-(trimethylsilyl)propionic acid-d4 sodium salt (0.05 w/v%, Sigma) as an internal standard (Figure S20 and Table S1).
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bioRxiv - Neuroscience 2021Quote: ... A gelling solution was made by combining monomer solution with 0.01% (w/v) 4-hydroxy-TEMPO (4HT, Sigma 176141), 0.2% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... Mechanical dissociation using a gentleMACS Octo dissociator (Miltenyi) was performed followed with enzymatic digestion with collagenase I (0.05% w/v, Sigma), DNase type IV (30 U/ml ...
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bioRxiv - Microbiology 2022Quote: Freshly dissected untreated clampcKO sporozoites were lysed on ice for 30 min in a lysis buffer containing 0.5% w/v NP40 (Igepal CA-630, Sigma) and protease inhibitors ...
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bioRxiv - Microbiology 2022Quote: ... after blocking of the slides for 1 h with HBS buffer (10 mM HEPES pH 7.4, 150 mM NaCl) containing 1 % (w/v) BSA (Sigma), 0.02 % (w/v ...
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bioRxiv - Microbiology 2022Quote: ... M9 minimal media (0.1 mM CaCl2, 2mM MgSO4, 1x [Enco-teknova] trace metals, 1% [w/v] glucose, 1x (Sigma) M9 Salts) ...
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bioRxiv - Molecular Biology 2022Quote: ... a locally produced viral transport medium (REVITAL Healthcare, Kenya) (RVTM), 1X Phosphate Buffered Saline (PBS) (OXOID, Hampshire, England) and 0.9% (w/v) sodium chloride (NaCl) (SIGMA). Five vials (2 high concentration SARS-CoV-2 positive samples ...
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bioRxiv - Cancer Biology 2022Quote: ... and resuspended in a 1:1 mixture of fresh media and reconstitution buffer [2.2% w/v NaHCO3 (Sigma Aldrich) and 4.8% w/v HEPES (Acros ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Bioengineering 2022Quote: ... and 1 % (w/v) alizarin red (pH 4.1) to determine mineral deposition via calcium staining (all from Sigma-Aldrich). Stained sections were imaged using an Aperio ScanScope slide scanner.
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bioRxiv - Bioengineering 2022Quote: ... poured into a sterile 750mL bottle along with 6% w/v PEG-8000 (Sigma-Aldrich, cat. code P2139-500G) and 3% w/v NaCl (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS for 10 min and subsequently blocked in 5% (w/v) BSA (Sigma-Aldrich, St Louis, MO, USA) with 0.03% Triton-X in PBS for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... we filtered cell suspensions through a 100μm cell strainer and added 3ml 0.1% w/v soybean trypsin inhibitor to stop the digest (Sigma# T9128). The suspensions were then filtered through a 40μm cell strainer ...
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bioRxiv - Immunology 2022Quote: ... Tissues were minced into approximately 0.2-0.3 mm3 cubes and enzymatically digested in 0.5mg/mL collagenase V (Sigma, C-9722) solution in 50 mL R3 media (RPMI 1640 with 3% FBS ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (10 μL) were visualized by gel electrophoresis in 1% (w/v) agarose gels (Sigma-Aldrich, Darmstadt, Germany). Sequencing was performed (LGC Genomics GmbH ...
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bioRxiv - Microbiology 2022Quote: ... Fixed bacteria were washed with PBS and a 5 μl suspension was then centrifuged onto coverslips pre-coated with 0.01% (w/v) poly-L-lysine (Sigma) in a 24-well tray (16,000 ×g ...
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bioRxiv - Plant Biology 2023Quote: Seed coat permeability was assessed by staining seeds with 1% (w/v) 2,3,5-triphenyltetrazolium chloride (Sigma-Aldrich; https://www.sigmaaldrich.com) solution at 30 °C in the dark for the time indicated in each figure ...
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bioRxiv - Neuroscience 2022Quote: ... mice were anesthetized and administered fluorescein isothiocyanate (FITC)–dextran (0.1 ml, 2 M MW, 5% w/v, Sigma-Aldrich) via tail veil to label blood vessels ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... followed by blockage with mixture buffer (1% w/v BSA, Sigma-Aldrich, USA; 22.52 mg/ml glycine in PBST). The cells were incubated with mouse monoclonal Cy3-conjugated anti-GFAP (1:400 ...
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bioRxiv - Plant Biology 2022Quote: ... the embryonic structures were allowed to develop further by transferring the explants to half MS medium with 1 % (w/v) sucrose and 0.7 % agar (Sigma) without hormones ...
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bioRxiv - Plant Biology 2022Quote: Individual isolates were cultured on a 1/10 dilution of tryptic soy broth (TSB, Difco) agar (1.5%, m/v) media containing 50 mg/L thiabendazole (Sigma) and incubated for 48 hours at 26°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Experiments were conducted at 3–5 days post-fertilization (dpf) unless otherwise stated and under anesthesia using 0.01% (w/v) tricaine methansulfonate (MS-222, Sigma).