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Citations for Millipore Sigma :
5751 - 5800 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 18 μL (1:3 ratio of DNA to transfection reagent) of X-tremeGENE™ 9 DNA transfection reagent (MilliPore Sigma). The mixture was incubated for 30 mins at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... pH 8) containing 3 mg/ml lysozyme (Chicken egg white; Fluka) and 1 mg/ml lipase (from Candida cylindracea; Sigma) in an Eppendorf tube ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed cells were stained with the following antibodies (for 3 hrs at 37°C): β-Tubulin (1:100, Sigma-Aldrich), DBC1 (1:100 ...
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bioRxiv - Cell Biology 2019Quote: ... washed 3 times in PBS and then incubated for 30 min with secondary antibodies and DAPI (Sigma, 1:1000 dilution); all antibodies were diluted in PBS + 3% BSA and are as follows ...
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bioRxiv - Biochemistry 2019Quote: ... 50ml fractions were collected and DJ-1/Hsp32-containing fractions (eluted after 150–200 ml) were concentrated using a 3-kDa Amicon Ultra column (Millipore). Concentrated DJ-1/Hsp32 was loaded onto a gel filtration column (Superdex 200 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were grown in M9 minimal media supplemented with glucose containing M9 salts (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl) (Sigma-Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Bioengineering 2020Quote: ... the chips were silanized by immediately adding 0.5 ml of a fresh 1% aqueous solution of 3- (Ethoxydimethylsilyl) propylamine (Sigma, 588857) into the culturing chamber of the chip and incubated for 15 min at RT before rinsing with twice with 1 ml dH2O water ...
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bioRxiv - Physiology 2019Quote: ... cells were kept in 5 mM glucose containing DMEM medium for 3 hours followed by 1 hour in serum free EBSS (Cat. No # E2888, Sigma) prior to collection ...
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bioRxiv - Microbiology 2020Quote: ... parahaemolyticus cells incubated in ASW microcosms at 4°C for 100 days were re-suspended in 50 mM potassium phosphate buffer (pH 7) containing 1 g of 3-mm-sized glass bead (Sigma), vortexed for 25 min ...
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bioRxiv - Neuroscience 2019Quote: ... then blocked for 1 hour in PBS supplemented with 0.5% Triton X-100 and 3% normal Goat Serum (Sigma G9023). Slices were incubated at 4°C in primary antibody (diluted in blocking solution ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 mg/mL X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside, Sigma Aldrich Aldrich, St. Louis, MO), and 150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)11 (Vero passage 3) was kindly provided by CDC and propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... The animals were then subjected to increasing doses of methacholine (0.1, 0.3, 1, 3, 10, 30mg/mL) (a broncho-constrictor; acetyl-β methacholine chloride; Sigma-Aldrich) delivered by an ultrasonic nebuliser ...
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bioRxiv - Genomics 2021Quote: ... Cells were pelleted at 600 g for 3 min and resuspended in 400 μl Wash Buffer 1 (wash buffer with 0.05 % saponin (Sigma Aldrich), protease inhibitor cocktail (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)49 (Vero passage 3) was kindly provided by the CDC and propagated once in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Bioengineering 2021Quote: ... the precursor solution for the Zn0.4Fe2.6O4 nanoparticles was prepared at a con-centration of 0.2 molZn+Fe / L in a 3:1 volume ratio of xylene and acetonitrile (both Sigma-Aldrich). Iron acetylacetonate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and aphidicolin (7.5µM) before cells were treated for 3 hours with nutrients (1 µM insulin plus 0.8 mM L-leucine [Sigma Aldrich catalog numbers I5500 and L8000 ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in the presence of Zombie Red (1/1000) according to the manufacturer’s procedure (Ozyme) then trypsinized and fixed in PBS with formalin 3% (Sigma Aldrich). For F-actin quantification ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected using X-tremeGENE 9 (ratio 3:1 μl/μg DNA) according to the manufacturer’s protocol (Sigma-Aldrich). DNA encoding PAC constructs and SthK-EGFP was used at a 3:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... media (ratio 1:3) for 30-45 min until the digestion was stopped by the addition of FBS (Sigma, Germany) at a 10 % v/v ratio ...
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bioRxiv - Neuroscience 2020Quote: ... animals were mounted by placing them in 3% agarose pads on glass slides and immobilized with 1 mM tetramisole hydrochloride (Sigma). Imaging was performed using Confocal Laser Scanning Confocal microscope (Olympus 1X61 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then solubilized by mixing 3:1 lysate to Li-Cor 4x loading buffer (928-40004) plus 10% Beta-Mercaptoethanol (Sigma). Samples were denatured by boiling for 5 min followed by cooling on ice for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was performed on 12% SDS-PAGE gels using 1-hour semi-dry transfer with 3% bovine serum albumin (BSA, Sigma) in TBS + 0.5% Tween-20 as a blocking agent.
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Tg[elavl3:Gal4;10xUAS:jRGECO1b] at 3 dpf were paralyzed by a 5-min bath application of 1 mg/ml a-bungarotoxin (Sigma, 203980). Larvae were mounted on their side in a field stimulation chamber (Warner ...
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bioRxiv - Microbiology 2020Quote: ... The plasma-treated coverslips were then transferred into 150 mL of 1% (v/v) (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich) in toluene (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) 18:1 PS (DOPS) was purchased from Sigma-Aldrich. N-((6-biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanoamine ...
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bioRxiv - Developmental Biology 2019Quote: ... 1-octen-3-ol and acetophenone) larvae were treated with respective odorants of the highest available purity (>99% from Sigma) Acetic acid (Sigma 2722) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were arrested in G1 as above and 1 h prior to release auxin (indole-3-acetic acid sodium salt; Sigma) was added to a final concentration of 1 mM ...
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bioRxiv - Cell Biology 2019Quote: ... cells were seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips coated with poly-L-lysine (3 min application of 0.1 mM polylysine (Sigma P2636) in borate buffer ...
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bioRxiv - Pathology 2019Quote: ... all drug treatments (1 μM Cysteamine; Sigma, 100 nM Everolimus; RAD001 Selleckchem, 30 mM 3-methyladenine; Sigma, 50 mM Sucrose; Sigma) were added to cell culture medium and incubated with the cells for 24 hrs.
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bioRxiv - Neuroscience 2019Quote: ... the sections were washed (3 × 5 min) in 0.1 M PBS and incubated with goat anti-rabbit Alexa488 (1:500; Sigma-Aldrich) secondary antibody (to label collagen-I ...
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bioRxiv - Molecular Biology 2020Quote: 25 mm round cover glass (Fisher Brand, #12-545-102 25CIR-1) was coated with 3-Triethoxysilypropylamine APES (Millipore, #A3648) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with Small molecule inhibitors PD0325901 (PD, 1 μM, Selleck) and CHIRON99021 (CH, 3 μM, Selleck) and 1000 U/ml LIF (ESGRO, Millipore). Cells were routinely propagated on 0.1% gelatin-coated plastic and replated every 3 days at a split ratio of 1 in 10 following dissociation with Accutase (Gibco ...
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bioRxiv - Genetics 2021Quote: In vitro treatments were carried out with different compounds: 1 μM MG-132 24 hours or 3 hours (Merck Millipore), 20 μM Chloroquine (CQ ...
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Synthetic Biology 2021Quote: ... and M9 minimal media supplemented with glucose (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl (Sigma-Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...
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bioRxiv - Immunology 2019Quote: ... Mouse neutrophil was isolated from the peritoneal cavity 3 hours after injection of 1 ml thioglycollate medium (Sigma-Aldrich, MO). Isolated neutrophils were incubated at 37C for 6 hours and then treated with 10 ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was exchanged for differentiation medium I [supplemented DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 0.25 μM dexamethasone (Dex ...
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bioRxiv - Immunology 2021Quote: ... Cy5- or Cy7-PMPC25-PDPA68 copolymer was dissolved together with the PMPC25-PDPA68 in an organic solution of 3:1 (v/v) methanol:tetrahydrofuran (Sigma-Aldrich) for a final copolymer concentration of 20 mg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... and transcription factor SREBP1a (aa 1-50) were generated by inserting the corresponding cDNA fragments into pGEX4T-3 vector (Sigma). V5-tagged full-length and N-terminal truncation (Δ302 ...
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bioRxiv - Cell Biology 2020Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...