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Citations for Millipore Sigma :
5551 - 5600 of 10000+ citations for 6 PHENYL 1 3 DIHYDRO INDOL 2 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... rabbit Anti-Phospho-PAK4/5/6 (pSer474) (Sigma-Aldrich, SAB4503964); rabbit Anti-Phospho-14-3-3 (pSer58 ...
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bioRxiv - Genomics 2024Quote: ... 6) 10 seconds in 5% Eosin Y solution (Sigma 318906), 7 ...
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bioRxiv - Plant Biology 2024Quote: ... ½ MS (Duchefa Biochemie) and 0.5 uM 6-benzyl aminopurine (Sigma), adjusted to pH5.7 using 0.5 M KOH (Sigma).
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bioRxiv - Genomics 2021Quote: ... PTA was carried out by first further lysing the cells after freeze thawing by adding 2 μL a prechilled solution of a 1:1 mixture of 5% Triton X-100 (Sigma-Aldrich) and 20 mg/ml Proteinase K (Promega) ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: After 4 hours cells were washed twice with 1 ml Neurobasal medium and changed subsequently to 2 mL of retinoic acid medium made of 1:1 minimal essential medium (MEM) (Sigma-Aldrich) and nutrient mixture F-12 Ham (Sigma-Aldrich ...
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GFI1 cooperates with IKAROS/IKZF1 to activate gene expression in T-cell acute lymphoblastic leukemiabioRxiv - Biochemistry 2021Quote: ... and viral envelope (pMD2G) constructs at a 4:2:1 ratio with 1 mg/mL polyethylenimine (PEI, linear MD 25,000 Da, Sigma, Cat# 408727). The ratio of total transfected DNA to PEI was 1:3 (1 µg DNA:3 µg PEI) ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody, Sigma in 2% milk Tris – buffered saline ...
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bioRxiv - Neuroscience 2020Quote: ... cells were thereafter maintained in glia-conditioned NM1 (Neurobasal media with 2mM GlutaMax, 1% FBS, 2% B27, 1 x FDU (5mM Uridine (SIGMA F0503), 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003) ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2019Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-DmEb1 (gift from H ...
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bioRxiv - Cell Biology 2021Quote: ... Staining reagents: anti-tubulin (clone DM1A, mouse, 1:1000, Sigma; alternatively, clone YL1/2, rat, 1:500, Millipore Bioscience Research Reagents); anti-Syt (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... or (2) primary anti-dsRed (Sicgen, AB8181-200, 1:200) together with primary rabbit anti-proSP-C (Sigma, AB3786, 1:1000) or primary rabbit anti-NaPi-IIb (provided by C ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed with PBS and incubated in post-infection media (DMEM supplemented with 2% FBS, 1% PSG) containing 1% microcrystalline cellulose (Avicel, Sigma-Aldrich) at 37°C in 5% CO2 for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... and then mixed 1:1 with 500 μl Diluent-C containing 2 μl CellVue Claret Far-Red Fluorescent Membrane Linker (Sigma MINCLARET), labeling mixture that was incubated for 10 min at room temperature in the dark ...
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bioRxiv - Microbiology 2024Quote: The bacterial culture was washed once with 1×PBS and then added to 1 mL of 1/2 TSB medium supplemented with or without 0.4 mM IAA (Sigma-Aldrich, USA) to achieve a final OD600 of 0.05 ...
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bioRxiv - Neuroscience 2023Quote: Plastic sections 1 µm thick from embryonic and adult OT and adult retina mounted on glass slide were stained by incubation 1-2 minutes in 1% toluidine blue (Sigma Aldrich), 1% sodium borate in distilled water ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μL followed by 1 mL of ice-cold buffer (PBS, 2% FBS, 1 mM EDTA) containing protease inhibitors (Sigma, #P1860) was injected into the lung ...
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bioRxiv - Developmental Biology 2023Quote: ... Truncated FBF expression constructs were generated by PCR from full-length FBF-1 or FBF-2 and inserted into pETDuet-1 plasmid (EMD Millipore). Full-length C ...
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bioRxiv - Neuroscience 2023Quote: ... cells were thereafter maintained in glia- conditioned NM1 (Neurobasal media with 2mM GlutaMax, 1% FBS, 2% B27, 1 x FDU (5mM Uridine (SIGMA F0503), 5 mM 5-Fluro-2’-deoxyuridine (SIGMA U3003) ...
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bioRxiv - Cell Biology 2023Quote: ... ‘blocking solution’ made up of PBS supplemented with 2% BSA (A1391, AppliChem) and 1% Triton X-100 (9002-93-1, Sigma-Aldrich) was added to the fixed cells for 30min at RT ...
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bioRxiv - Immunology 2023Quote: ... blood was diluted 1:2 in PBS and peripheral blood mononuclear cells (PBMCs) were isolated using Histopaque (Sigma, Cat# 1077-1) gradient ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Cell Biology 2024Quote: ... dilution 1:10) cell-surface antibodies after a non-specific sites’ saturation step with Gamma Immune (Sigma Aldrich, dilution 1:2). Isotype controls (Miltenyi Biotech) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed with PBS and lysed in an immunoprecipitation buffer reported to partially solubilize keratins (40 mM HEPES, 120 mM sodium chloride, 1 mM EDTA, 1% Triton X-100, 2% Empigen BB [Sigma-Aldrich #30326] ...
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bioRxiv - Biochemistry 2022Quote: ... or 1:5000 anti-Rabbit IgG HRP Linked F(ab′)2 (Sigma, NA9340V, Lot. 17065618) was added for 1 hour and membranes were imaged using Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Physiology 2019Quote: ... at 27°C in 2 mL BSA-free ECS with 1 μL 20% Pluronic (Sigma), followed by 20 min in ECS with 0.1% BSA to de-esterify the dyes ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mM EDTA) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease inhibitor cocktail (Sigma). Lysates (20 μg/lane ...
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bioRxiv - Genetics 2019Quote: ... 50 mM NaF supplemented with protease (PMSF) and phosphatase inhibitors (Sigma cocktails 1 and 2), together with glass beads ...
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bioRxiv - Systems Biology 2020Quote: ... A standard stock solution containing 1% 2-methyl pentanoic acid (Sigma-Aldrich St. Louis, MO) was prepared as an internal standard control for the volatile acid extractions.
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bioRxiv - Immunology 2019Quote: ... and finally cleared with a 1:2 solution of benzyl alcohol: benzyl benzoate (BABB; Sigma) before mounting between No ...
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bioRxiv - Cell Biology 2020Quote: ... 25 mM NaF and 2 mM NaH2PO4 containing 1% protease inhibitor cocktail (P8340, Sigma-Aldrich) and 1% phosphatase inhibitor cocktails 2 and 3 (P5726 and P0044 ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial pellet was lysed in 2 mL 1× BugBuster™ protein extraction reagent (Novagen), incubated by slow shaking for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... pH 7.25 with KOH (275 mOsm) and 1-2 mg/mL biocytin (B4261, Sigma-Aldrich). Open tip resistance was 2.5-4 MΩ ...
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bioRxiv - Cancer Biology 2020Quote: ... CALCRL MISSION shRNA (shCALCRL#1, Sigma-Aldrich, Cat# TRCN0000356798; shCALCRL#2, Sigma-Aldrich, Cat# TRCN0000356736), E2F1 MISSION shRNA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Marrow stromal cells were cultured in the presence of ascorbate-2-phosphate (1 mM) (Sigma). Colony forming units-fibroblastoid (Cfu-f ...
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bioRxiv - Systems Biology 2020Quote: ... 1% penicillin-streptomycin glutamine (Wisent #450-202-EL) and 2 µg/mL puromycin (Sigma #P8833).
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bioRxiv - Microbiology 2020Quote: ... cholerae were spotted on 1-2% agarose pads of M9 supplemented with 0.5% glucose (Sigma) and incubated at 25°C throughout the experiment ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then transferred in a Benzyl Benzoate/Benzyl Alcohol solution (Sigma Aldrich, 2:1) and stored in dark at 4°C until imaging.
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bioRxiv - Microbiology 2021Quote: ... non-pretreated and pretreated RAW264.7 cells were treated with 2-DG (1 mM, Sigma-Aldrich), sodium dichloroacetate (10 µM ...
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bioRxiv - Microbiology 2021Quote: Rheological measurements of 1% MC+2% LB solution and 18.7% (w/w) mucin (Sigma, USA) in 2% LB solution were carried out on a rheometer (DHR-2 ...
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bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
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bioRxiv - Cancer Biology 2021Quote: ... RIPA buffer was supplemented with Phosphatase Inhibitor Cocktail 2 solution (diluted 1/100, SIGMA-Aldrich). A 24-well plate was layered with 20 μl of the 2.2 mg/ml unlabeled type I collagen polymerization mix as described above ...
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bioRxiv - Biochemistry 2022Quote: ... L-1- Tosylamido-2-phenylethyl chloromethyl ketone (TPCK) treated Trypsin (Sigma-Aldrich; cat. no. T1426) was added to the βarr1 phospho-peptide mixture at a ratio of 1:25 and 1:50 (w/w ...
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bioRxiv - Bioengineering 2022Quote: ... Anesthetic agarose was prepared by mixing 2% agarose in 1% chloretone (Sigma-Aldrich Cat# 112054) dissolved in 1× Instant Ocean supplemented with 1:5000 linalool ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were counterstained with 1 ug/ml 4,6-diamidino-2-pheenylindole (DAPI, Sigma cat #D9542) and mounted with Prolong Gold (Thermo Fisher Scientific cat# P36930) ...
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bioRxiv - Bioengineering 2022Quote: ... at 4°C for 2 h and blocked with 1% bovine serum albumin (Sigma-Aldrich), 3% donkey serum (Millipore) ...
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bioRxiv - Genetics 2019Quote: ... 0.1% 2-mercaptoethanol) supplemented with a protease inhibitor cocktail (1 tablet per 50 ml, Sigma). Total KCC4 antibody used was previously described (Adragna ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Immunology 2019Quote: ... MTT (1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan) and paraformaldehyde were obtained from Sigma Aldrich Co (St Louis ...
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bioRxiv - Molecular Biology 2020Quote: ... For FLAG ChIP-seq experiments 1-2 μl of FLAG M2 antibody (Sigma-Aldrich, #F3165) were used per IP and 4-5 IPs (equivalent to 10-12 million cells ...