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Citations for Millipore Sigma :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression in E.coli was stimulated by IPTG (Isopropyl β-D-1-thiogalactopyranoside, Sigma-Aldrich) at 1 mM at 16°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Mediator complex in the nuclear extract was affinity-purified by anti-FLAG M2 Affinity gel (Sigma-Aldrich, A2220) and washed extensively by BC100 buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... digested with lysozyme at a concentration of 10 µg/mL (Sigma-Aldrich) for 30 min and ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were selected with Phleomycin (Sigma-Aldrich / Merck) at 2.5 μg/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... and alcohol dehydrogenase (150 kDa) (Sigma-Aldrich). rFhENO (100 µg ...
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were grown in the presence and absence of 1 μg/ml tetracycline (Sigma-Aldrich / Merck) for RNAi induction ...
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bioRxiv - Genetics 2024Quote: ... supplement with 10% Fetal Bovine Serum (Sigma Aldrich, F7524) and 1% penicillin/streptomycin (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Nuclei were stained with 1:10.000 dilution of DAPI 0.2 mg/mL (#D9542, Sigma-Aldrich) in 2x SSC for 3 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µM of 4-NQO (Sigma-Aldrich) was added to cell culture media for 4 hr before cell harvest.
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bioRxiv - Molecular Biology 2024Quote: FLAG-Rhino inducible OSCs were cultured in Shields and Sang M3 Insect Medium (Sigma) supplemented with 10% fly extract ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was resuspended in 6 µl of deionized formamide (#F9037, Sigma) and dissolved for 15 min at 37°C with agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of probe was precipitated using 1/10 3M NaAc (#S2889, Sigma-Aldrich), sheared salmon sperm DNA (#AM9680 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated onto poly-L-lysine (#P4832, Sigma)-coated 22×22 mm coverslips (#0101050 ...
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bioRxiv - Molecular Biology 2024Quote: ... and CD9 (Millipore, Darmstadt, Germany), then visualized with SuperSignal Extended Duration Substrate (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... complemented with 1% polybrene (Millipore, Billerica, MA).
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bioRxiv - Molecular Biology 2024Quote: ... and anti-beta-actin (A2228, Sigma-Aldrich). After washing ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using a 0.45-μm filter (Millex-AA; Merck Millipore, Darmstadt, Germany). The filtered viral particles were centrifuged at 8,000× g for 35 min at 4°C ...
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bioRxiv - Genomics 2024Quote: ... the RNA samples were extracted with phenol/chloroform (Sigma). The sample was centrifuged at 4°C and 7000 x g for 25 min ...
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bioRxiv - Genomics 2024Quote: ... Fertilized embryos were collected from the plugged females at 1.5 dpc (2-cell stage) by flushing M2 (Millipore) through the infundibulum and out the uterine horn using a blunt needle ...
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bioRxiv - Genomics 2024Quote: ... followed by 5IU HCG (Sigma Aldrich) 46-48 h later ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Genomics 2024Quote: ... coated with poly-L-lysine (Sigma). Embryos were imaged with a Zeiss LSM800 ...
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bioRxiv - Genomics 2024Quote: ... the embryos (8-16 cell stage) were washed through M2 and then placed in drops of Tyrode’s solution (Sigma). After about 30 sec ...
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bioRxiv - Genomics 2024Quote: ... at MOI of 20 (Stock: 3.5e10 TU/ml) along with polybrene (4ug/ml) (Sigma) for 24h before being replaced with a fresh medium ...
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bioRxiv - Genomics 2024Quote: ... All drops of KSOM were covered with mineral oil (Sigma). On the morning of the aggregation ...
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bioRxiv - Genomics 2024Quote: ... For poly I:C (Sigma P1530), we prepared a working stock at 5 mg/ml in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-G3BP1 (WH0010146M1, Sigma), anti-DCP1B (13233 ...
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bioRxiv - Molecular Biology 2024Quote: ... and protease inhibitor (Sigma P8340) at pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... we used the following secondary antibodies: anti-rabbit IgG Cy3-conjugated (Sigma-Aldrich C2306), anti-mouse IgG Cy3-conjugated (Sigma-Aldrich C2181) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-PMP70 (SAB4200181, Sigma), AP-1 (610385 ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were permeabilized for 20 minutes with 0.1% Triton X-100 (Sigma-Aldrich)/1xPBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and the supernatant was subjected to IP with 10 µL of packed anti-FLAG M2 magnetic beads (Sigma-Aldrich M8823) per mg proteins for 2 hours at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG Cy3-conjugated (Sigma-Aldrich C2181), anti-rabbit IgG Cy5 conjugated (Invitrogen A10523) ...
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bioRxiv - Molecular Biology 2024Quote: ... and also supplemented with penicillin/streptomycin (P/S) (Sigma-Aldrich, Extended Data Fig. 2b). Cells were maintained in 100-mm cell culture dishes (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... and 750 μL of buffer 1 (containing an undisclosed (somewhere between 50-70% (w/w)) amount of Guanidinium Hydrochloride (EMD Millipore, DE) dissolved in ultra-pure H2O ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% FBS (Sigma-Aldrich) and 1% P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... Phase separation was initiated by adding 200 µL (0.2× volumes) of 100% chloroform (Sigma-Aldrich) into 1-mL TRIzol-dissolved cell mixture ...
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bioRxiv - Genomics 2024Quote: ... 0.15 mM Monothioglycerol (Sigma, M6145-25ML), 1000 U/mL LIF (Cell Guidance Systems ...
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bioRxiv - Molecular Biology 2024Quote: ... adults were mounted on 2% agarose pads with 2 μL of 0.1% tetramisole (Sigma) in M9 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.1 μg of anti-Flag antibody (M2; Sigma‒Aldrich) was added to each tube ...
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bioRxiv - Molecular Biology 2024Quote: ... and one drop of antifoam 204 (Sigma). The cells were incubated at 23°C and 10 mL of culture was collected at various time points.
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bioRxiv - Molecular Biology 2024Quote: ... Fixed embryos were washed in 1 mL Stellaris Wash Buffer A (LGC Biosearch SMF-WA1-60) with 10% formamide (Sigma F9037) for 5 minutes and hybridized with 100 μL of Stellaris Hybridization Buffer (LGC Biosearch SMF-HB1-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... were prepared in sterile dimethyl sulfoxide (DMSO) (Sigma-Aldrich). For metabolic labeling ...
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bioRxiv - Molecular Biology 2024Quote: ... 2b–f 3b,c and 4b,c and Extended Data Fig. 1b,c, 2a,c,d, 3a and 4d,e; Sigma-Aldrich, Extended Data Fig. 2b), and also supplemented with penicillin/streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with Tween 20 (Sigma-Aldrich, MA, USA), EDTA (Carl Roth ...
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bioRxiv - Molecular Biology 2024Quote: ... amount dissolved in isopropanol (Sigma-Aldrich, MA, USA)) was added to each sample which was mixed ≥10x until a homogenous solution was reached ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1x anti-protease cocktail inhibitors (Sigma, P8340). Lysates were centrifuged for 20 minutes at 14,000 rpm at 4 °C to pellet insoluble material and chromatin ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg/ml insulin (Sigma, I5500) and 500 μM isobutylmethylxanthine (Sigma ...