Labshake search
Citations for Millipore Sigma :
5151 - 5200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: Determination of the dNTP pool size in each extract was as follows: 10 pmol template oligo (Sigma), 10 pmol probe (IDT ...
-
bioRxiv - Molecular Biology 2024Quote: ... Excess IR800-streptavidin was washed from the membranes by four times with 0.1% TWEEN-20 (Sigma-Aldrich) in 1× PBS for 10 min/each at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... Supernatants were adjusted to a final concentration of 150 mM NaCl and then incubated overnight with rotation at 4°C with either anti-Flag beads (Sigma, A2220) or with 1-5 μg of the appropriate antibody and then with Protein G Sepharose beads (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 0.5% glutaraldehyde (Sigma, G5882) in PBS for 15 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... 319-005-CL) supplemented with 10% foetal bovine serum (FBS, Wisent, 098150) or in 5% new-born calf serum (NBS, Sigma®, N4637) with 2% FBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Exponentially proliferating cells were grown to confluence and then left at confluence for 48 hours before incubation in differentiation media (DMEM supplemented with 10% FBS, 4 mM L-Glutamine, 100 U/ml penicillin/streptomycin, 1 μM dexamethasone (Sigma, D-2915), 1 μg/ml insulin (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... One milliliter of equilibrated HIS-Select Nickel Affinity Gel (Sigma) was added to the cleared cell lysate and incubated with mild agitation for 1 h at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM Na3VO4 (Sigma, S6508), 50 mM NaF (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 20 µM of MG132 (Sigma, C221) or 20 µg/ml of cycloheximide (Bioshop ...
-
bioRxiv - Molecular Biology 2024Quote: ... Hybond-C (Cytiva) or Amersham™ Protran® (Sigma-Aldrich) membranes were used ...
-
bioRxiv - Molecular Biology 2024Quote: ... The viral supernatant was filtered through 0.45 µm filter and added to the target cells along with 8 µg/ml polybrene (Sigma, H9268). Following one to three infections ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were incubated with GSH beads (Sigma, G4510) at 4°C for 5 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM of UDP-GlcNAc (Sigma, A8625) and 3 or 5 µg of purified His-OGT and mixed with either 3 or 5 µg of GST-FOXK1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 500 μM isobutylmethylxanthine (Sigma, I5879). Two days post-induction ...
-
bioRxiv - Molecular Biology 2024Quote: ... FOXK1 siRNAs (SASI_Hs01_00149056: GAUUGUAUGAUUCUGGGAA) and (SASI_Hs01_00149058: CUCUCUUUGAACCGUUACU) were obtained from Sigma-Aldrich.
-
bioRxiv - Molecular Biology 2024Quote: ... were washed three times with 3 ml of water using Amicon Ultra-4 (3 kDa cut-off, Millipore) (centrifugation at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... 2 mM VRC (Sigma-Aldrich), 0.2 mg/ml RNase-free bovine serum albumin (BSA ...
-
bioRxiv - Genomics 2024Quote: ... cells were transfected with X-tremeGENE HP DNA transfection reagent (Millipore Sigma, St. Louis, MO) using 10 µg of plasmid library and 40 µL reagent in 1000 µL Opti-MEM (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2024Quote: ... Cells were then treated with 2mM Thymidine (Sigma T1895) for 22 hours followed by a wash in 1xPBS and a 6 hour release ...
-
bioRxiv - Genomics 2024Quote: ... 100ng/mL of Nocodazole (Sigma M1404) was added to the media for 4 hours followed by a mitotic shake-off ...
-
bioRxiv - Genomics 2024Quote: ... Mission shRNAs (Sigma-Aldrich) were used for RNA interference ...
-
bioRxiv - Genomics 2024Quote: ... potassium chloride (Scharlau, cat. no. 0401, 2-mercaptoethanol, 14 M (Sigma, cat. no. M3148), 0.5 M ethylene diamine tetra acetic acid (Biobasic Inc. ...
-
bioRxiv - Genomics 2024Quote: ... refrigerated centrifuge equipped with fixed angle rotor (Sigma Model 4-16K), Steriflip vacuum-driven filtration system with 20 µm nylon net filter (Merck Millipore ...
-
bioRxiv - Genomics 2024Quote: ... 500,000 pre-stimulated CD34+ cells were suspended in 1 ml viral supernatant and 1 ml prestimulation medium with 5 μg/ml protamine sulfate (Sigma). The cells mixture was added to the RetroNectin coated plate and incubated at 37°C in 5% CO2 humidity for 2 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... duttoni (NIH Rocky Mountain Laboratories, Hamilton, MT) were grown in BSK-H medium with 6% rabbit serum (Sigma Aldrich, St. Louis, MO) until the culture reached log phase (∼4 days) ...
-
bioRxiv - Molecular Biology 2024Quote: ... total RNA was extracted from individual insects using Trizol reagent (Sigma-Aldrich, Burlington, MA).
-
bioRxiv - Molecular Biology 2024Quote: ... and cloning of it into NdeI-XhoI site of expression plasmid pET-22b(+) (Novagen, WI, USA).
-
bioRxiv - Molecular Biology 2024Quote: ... 60% iodixanol (Optiprep; Sigma, D1556, St. Louis, MO) was added 1:1 to the EV/buffer solution and transferred to the bottom of a 5.5 mL ultracentrifugation tube ...
-
bioRxiv - Molecular Biology 2024Quote: ... After blocking in 2 % w/v bovine serum albumin (BSA, Sigma) in PBS at room temperature for 1 hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mM EDTA) supplemented with 2 mM phenylmethylsulfonyl fluoride (PMSF, Sigma) and cOmplete™ ...
-
bioRxiv - Molecular Biology 2024Quote: Total RNA from cells and aortas was extracted using TRIzol Reagent® (Sigma-Aldrich), according to the standard protocol previously described [61] ...
-
bioRxiv - Molecular Biology 2024Quote: ... Treatment with N,N’-hexamethylene bis(acetamide) (Millipore Sigma, Burlington, MA) (HMBA ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The purified protein was concentrated to 0.6 mg/ml using an Amicon centrifugal contentrator (EMD Millipore), flash-frozen in liquid nitrogen and stored at −80°C.
-
bioRxiv - Molecular Biology 2024Quote: ... 7μL β-mercaptoethanol (Sigma, M6250; stock 14.3 M), 100μL LIF (Chemico ...
-
bioRxiv - Molecular Biology 2024Quote: ... and transferred to a 0.45-µm PVDF membrane (Sigma, IPVH00010). Upon transfer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 4μL β-mercaptoethanol (Sigma, M6250; stock 14.3 M), 50μL LIF (Chemico ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS three times and incubated with Hoechst (Sigma 33258) for 10 min at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... Concentration–response curves were obtained in responses to increasing concentrations of acetylcholine (Ach, 10−9 to 10−5☐M; Sigma-Aldrich).
-
bioRxiv - Molecular Biology 2024Quote: ... The cells were seeded on poly-L-ornithine coverslips (Sigma-Aldrich) and cultured in a serum-free medium (Dulbecco’s Modified Eagle Medium/NutrientMixture F-12 [DMEM/F-12] consisting of N1 supplement ...
-
bioRxiv - Molecular Biology 2024Quote: ... glutamine (Sigma), and penicillin/streptomycin/mixture (Gibco) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and Alcian blue 8GX (Sigma, A-5268) with a pH adjusted to 2.5 and filtration after overnight stirring ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
-
bioRxiv - Molecular Biology 2024Quote: ... For liquid chromatography a SeQuant ZIC-pHILIC 5 μm Polymeric column 150 * 2.1 mm (Merck Millipore, Darmstadt, Germany) was used ...
-
bioRxiv - Molecular Biology 2024Quote: ... The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041, Sigma) dissolved in TBST (0.05% tween) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Tubulin (T5168, Sigma-Aldrich); RMND5A (custom ...
-
bioRxiv - Molecular Biology 2024Quote: ... After staining with secondary antibodies (conjugated with either Alexa Fluor 488 or 594; Millipore) and Hoechst 33342 (BD Biosciences) ...
-
bioRxiv - Molecular Biology 2024Quote: ... pH 9 (M5162, Sigma-Aldrich), then resuspended in 20 mM DMP (Dimethyl pimelimidate dihydrochloride ...
-
bioRxiv - Molecular Biology 2024Quote: ... HA (HA-7, H3663 Sigma-Aldrich); FLAG (M2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... to a final concentration of 0.1 U/μl at room temperature for 15 minutes and immunoprecipitated with anti-Flag magnetic beads (Millipore Sigma) at 4°C for 1 hour ...