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Citations for Millipore Sigma :
5051 - 5100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... DNase (Millipore Sigma Cat# D5025), and Dispase (BD Biosciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail, 1X Sigma Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluted immunoprecipitated protein was precipitated by adding TCA and Tween 20 (Sigma Aldrich, St. Louis, USA) to a final concentration of 10% and 0.5% respectively and incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and 50 nM dexamethasone (Sigma), 0.1 mM 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... ATP was measured using the Firefly Luciferase ATP Assay (Millipore Sigma) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Differential cell counts were determined manually from BALF cytospins stained with modified Giemsa (Sigma Aldrich, #GS500) to identify macrophages ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 mM 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt (Sigma), and 0.1 mM IBMX (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... cells with successful viral integration were selected in 25mL IMDM medium containing 1µg/mL puromycin (Sigma-Aldrich). Selection took place for 48 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... After final washing steps signals were developed using Immobilon Forte Western HRP substrate (Sigma) using G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1x antibiotic/antimycotic solution (Sigma). S-95xCGG and S-16xCGG cells were grown in DMEM medium containing certified tetracycline-free FBS (Biowest) ...
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bioRxiv - Genetics 2024Quote: ... and the nuclei were labeled with 4′,6-diamidino-2-phenylindole (DAPI, Sigma‒Aldrich, D9542). Image capture was performed by a laser scanning confocal microscope (Olympus ...
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bioRxiv - Genetics 2024Quote: ... trypsin (Sigma, T4799) and DNase (Promega ...
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bioRxiv - Genetics 2024Quote: ... larvae were anesthetized in 0.01% MS-222 (Sigma) and mounted in borosilicate glass capillaries using 0.75% low-melt agarose (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... Membrane was incubated for 30 minutes in 1% skim milk for blocking and afterward incubated with primary antibody for over-night (anti-PCNA: Sc65598-santa cruz. anti-Flag:F1804-Sigma Aldrich, anti-H3 ...
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bioRxiv - Genetics 2024Quote: ... This iPS cell line was obtained by reprogramming of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). Patients’ hiPSC cell lines were obtained by reprogramming of skin primary fibroblasts from ...
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bioRxiv - Genetics 2024Quote: ... 13dpf larvae were stained with a 0.2% calcein (Sigma) solution in fish water for 20 minutes followed by 3x 10-minute washes in fish water ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1x antibiotic/antimycotic solution (Sigma). All cells were grown at 37°C in a humidified incubator containing 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... using a combination of oligo(dT)23 and random hexamer primers (Sigma). The resulting cDNA was amplified in an AriaMX cycler (Agilent ...
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bioRxiv - Genetics 2024Quote: ... five gravid adult worms were placed on a coverslip with a dried 2% agarose pad on it and were covered with a layer of Halocarbon oil (Sigma, St Louis, MO) to prevent drying during the process ...
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bioRxiv - Molecular Biology 2024Quote: ... The following day membranes were washed 3-times with TBST for 7 min and incubated with corresponding solutions of anti-mouse (A9044, Sigma; 1:15,000) or anti-rabbit (A9169 ...
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bioRxiv - Molecular Biology 2024Quote: ... or anti-rabbit (A9169, Sigma; 1:20,000) antibodies conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in room temperature for 1 h in 5% skim milk (Sigma) in TBST buffer (Tris-buffered saline [TBS] ...
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bioRxiv - Molecular Biology 2024Quote: ... Actin (A5441, Sigma-Aldrich); and HMGCS1 (A-6 ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
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bioRxiv - Molecular Biology 2024Quote: ... or HA (HA-7, H9658, Sigma-Aldrich) then incubated for 1 hour at 4°C with Dynabeads protein G ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8 (S9640, Sigma-Aldrich) for 2 hours at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... and 100 units/mL of Benzonase nuclease (E1014, Sigma Aldrich) and incubated on ice for 30 minutes ...
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bioRxiv - Genetics 2024Quote: ... Slides were incubated in Sirius Red (Sigma, #365548 – 0.3g in 100ml of picric acid ...
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bioRxiv - Genetics 2024Quote: ... Other antibodies used were SOX9 (1:1000, Sigma-Aldrich AB5535), TMEM237 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... FLAG (M2, F1804, Sigma-Aldrich); WDR26 (ab85962 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8 (S9640, Sigma-Aldrich) for 2 hours at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... and thyroglobulin were purchased from Sigma Aldrich. VFLIP (a stable ...
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bioRxiv - Molecular Biology 2024Quote: ... Cas9 protein was purchased from Millipore Sigma (A36498). Transfection of sgRNA ...
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bioRxiv - Molecular Biology 2024Quote: ... and the lysate was incubated with anti-Flag (M2) resin (Millipore Sigma). After incubation at 4°C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... The YTHDC1 complex was purified using anti-Flag (M2) resin (Millipore Sigma) in TAP buffer (50 mM Tris-HCl pH 7.9 ...
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bioRxiv - Genetics 2024Quote: ... 1000 U/ml leukemia inhibitory factor (LIF, Millipore), 3 μM Gsk3 inhibitor CT-99021 ...
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bioRxiv - Genetics 2024Quote: mESCs were grown on 0.1% gelatin-coated flasks in serum-containing medium supplemented with 2i and LIF (2iL) (DMEM (Sigma), 15% ESC-grade FBS (Gibco) ...
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bioRxiv - Molecular Biology 2024Quote: ... selection with puromycin (in final concentration 1 μg/ml, Sigma) was initiated and lasted for 72h ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with pre-washed 50 μl of magnetic-streptavidin beads (MyOne C1, Sigma) for 1.5h in cold room rotating ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Molecular Biology 2024Quote: ... or stained for plaques with Thioflavin S (Sigma-Aldrich, 0.1% in 80% ethanol) for 8 min followed by three 2-min washes with 50% ethanol at room temperature ...
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bioRxiv - Genetics 2024Quote: ... the fat bodies of 3rd instar larvae were dissected in Schneider’s medium (Sigma) and placed at the center of the imaging dish in 20Lμl of Schneider’s medium ...
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bioRxiv - Molecular Biology 2024Quote: ... and embedded in 4% low melting agarose (melting temperature ≤65°C; Sigma) dissolved in distilled water ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% Protease Inhibitor Cocktail (P8215, Sigma-Aldrich), and 1× Complete Protease Inhibitor Cocktail (05056489001 ...
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bioRxiv - Molecular Biology 2024Quote: ... slides were washed with stringent buffer (1:1, formamide:4X SSC buffer; Thermo Fisher #AM9342; Sigma-Aldrich #S6639) at 37°C twice for 25 minutes each ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted using Tri-reagent (SIGMA) and reverse transcribed with Superscript II (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Acetonitrile (ACN) gradient chromasolv for HPLC and 2-propanol were purchased from Sigma. Ethyl acetate Scharlau was of HPLC grade and purchased from ICS (Lapeyrouse-Fossat ...
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bioRxiv - Immunology 2024Quote: ... Water from Milli-Q Advantage A10 and MultiScreen-HV 96-well Plates were used (Merck Millipore). Calibration ranges were 3.125 – 200 ng/mL for U and 25 - 500 ng/mL for UH2 and 5-FU (5µg/mL ...
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bioRxiv - Immunology 2024Quote: Cells were lyzed in RIPA buffer supplemented with protease inhibitors (AEBSF 4 mM, Pepstatine A 1 µM and Leupeptin 0.4 mM; Sigma Aldrich) and HIF-hydroxylase inhibitor (DMOG 1 mM ...
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bioRxiv - Developmental Biology 2024Quote: ... and 0.1% DNase (Sigma-Aldrich; DN25)] ...