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Citations for Millipore Sigma :
5101 - 5150 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The ligation product is column-cleaned (Sigma-Aldrich NA1020) and transformed using NEB Turbo Electrocompetent cells (NEB C2986 ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as doxycycline hyclate (Cat#24390-14-5) were obtained from Sigma. DYKDDDDK Epitope Tag (Cat#NBP1-06712 for WB) ...
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bioRxiv - Molecular Biology 2024Quote: ... Potential contamination among subcellular fractions was inspected by the presence of nucleus-specific marker Histone H3 on Western blot with human anti-Histone H3 antibody (ab1791) and mitochondria-specific activity of succinate dehydrogenase using the Succinate dehydrogenase activity colorimetric Assay (Sigma Aldrich), both performed according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... and stained using DAPI (Sigma-Aldrich, St. Louis, MO, USA). Images were obtained using a fluorescence microscope (Leica ...
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bioRxiv - Molecular Biology 2024Quote: ... stained with primary antibody overnight at 411°C (GFP 1:150 catalog no. Sigma #G1544), washed three times with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... anti-VLDLR antibody produced in rabbit (diluted 1:1000, Sigma-Aldrich), and control GAPDH antibody (diluted 1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... /ethidium bromide (Sigma-Aldrich, E1510) (AO/EB ...
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bioRxiv - Molecular Biology 2024Quote: ... cell viability was assessed using acridine orange (Sigma-Aldrich, A8097) /ethidium bromide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... the zona pellucida was removed by a brief incubation in drops of warm Acidic Tyrode’s solution (Sigma-Aldrich), followed by other two M2 washes to neutralize the acid ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by two additional PBS-T washes and then blocking - for 3 h at room temperature or overnight at 4°C - in BSA (Bovine Serum Albumin, Sigma-Aldrich #A2153) 3% in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-O-GlcNAc clone RL2 (abcam #ab2739 and Merck Millipore #MABS157), anti-O-GlcNAc clone HGAC85 (ThermoFisher #HGAC85) ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell lysate was then added to 1 mL of a Ni bead slurry (Ni Sepharose® High Performance; Catalogue #: 17-5268-02; Sigma-Aldrich, St. Louis, MO) and incubated overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... and cultured with medium containing 10 μM ROCK inhibitor Y-27632 (Sigma, Cat# SCM075) for one day ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspension of the microtubule pellet in BRB80 supplemented with 10 µM Taxol (Sigma Aldrich, T7191) (BRB80T).
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bioRxiv - Neuroscience 2024Quote: ... The flow cells were incubated with 20 µg/ml anti-biotin antibody (Sigma Aldrich, B3640) in PBS for 10 min and passivated by 1% Pluronic F-127 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... Goat anti-ChAT (1:200, Millpore) diluted in PBS supplemented with 2% horse serum and 0.1% triton X-100 (Sigma-Aldrich) were applied onto the sections followed by overnight incubation at 4°C in a humidified chamber ...
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bioRxiv - Neuroscience 2024Quote: ... or PVDF Membrane (Millipore). To prevent nonspecific binding ...
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bioRxiv - Neuroscience 2024Quote: ... in PBS for 10 min and passivated by 1% Pluronic F-127 (Sigma Aldrich, P2443) in PBS for at least 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred to PVDF membranes (Immobilon-FL, Millipore, IPFL00010). Membranes were incubated with blocking buffer (Intercept blocking buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then washed in PBS and incubated with the appropriate secondary antibody diluted in PBS containing 2% horse serum and 0.1% triton X-100 (Sigma-Aldrich) with Donkey anti-rabbit Alexa488 (1:200 Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2024Quote: ... Primaries were prepared in 10% normal goat serum and applied as combinations of either: 1) anti-Aβ1-40 rabbit polyclonal (Millipore Sigma #AB5074P, 1:200) and anti-Aβ1-42 12F4 mouse monoclonal (Biolegend #805502 ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.05 % Tween 20 and concentrated in Amicon centrifugal filter 50K (Millipore) to a final volume of 500 uL ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mg/mL Lysozyme (Sigma, L6876-10G), and Pierce Protease Inhibitor Tablets (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... After 72 hours the transfected Q7 cells were harvested with Trypsin (Sigma) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and stained with an anti-Ad hexon antibody (Sigma), whose binding was revealed with a horseradish peroxidase labeled rabbit anti-mouse antibody (Southern Biotechnology) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG (F3165, Monoclonal ANTI-FLAG M2, Millipore Sigma, St. Louis, MO), anti-FRB (ALX-215-065-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... A kanamycin-resistance sequence (Sigma-Aldrich) was centered between a left Ad6 homology arm (bp 29,290 to 29,340 ...
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bioRxiv - Neuroscience 2024Quote: ... Calbindin D-28K (Millipore Cat# AB1778, RRID:AB_2068336), MBP (Abcam Cat# ab40390 ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 µL of 1000x tetracycline hydrochloride (5mg/mL, Sigma-Aldrich T7660-5G) dissolved in ddH2O ...
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bioRxiv - Neuroscience 2024Quote: ... β2 Spectrin (BD Biosciences Cat# 612563, RRID:AB_399854, α-Tubulin (Sigma-Aldrich Cat# T6074, RRID:AB_477582), and β-Actin (Sigma-Aldrich Cat# A1978 ...
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bioRxiv - Neuroscience 2024Quote: ... and Calbindin D-28K (Millipore Cat# AB1778, RRID:AB_2068336, 1:1000). Chicken polyclonal antibodies against GFAP (Abcam Cat# ab134436 ...
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bioRxiv - Neuroscience 2024Quote: ... and were then immediately frozen using the combination of 2-Methylbutane (Millipore Sigma) and liquid nitrogen (for details see Tissue Preparation Guide CG000240 ...
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bioRxiv - Neuroscience 2024Quote: ... with an adhesive base and two 1.5 mm ports (with attached tubing connectors, Grace Bio-Labs press fit tubing connectors, Millipore Sigma, Cat. #GBL460003) on diametrically opposite sides for gas inlet and outlet ...
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bioRxiv - Neuroscience 2024Quote: ... we first intraperitoneally administrated 50 mg Kg-1 4-hydroxytamoxifen (4-OHT; Sigma, Cat# H6278) into TRAP2/Ai9 mouse line ...
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bioRxiv - Developmental Biology 2024Quote: ... Animals were anaesthetised in 0.03% benzocaine (Sigma) before amputation and surgery ...
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bioRxiv - Neuroscience 2024Quote: ... The iL3 was then transferred in a single droplet to a 2% Nematode Growth Media (NGM) agar plate [67] and paralyzed with nicotine (Sigma N3876, 1% in dH2O). The iL3 was then scored for activation based on the presence (activated ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the binding partners of F/M-tagged FOG1 in the EPO+CRISPR NC and EPO+CRISPR HSCB groups of K562 cells were purified by the Anti-FLAG® M2 Magnetic Beads (M8823, Sigma-Aldrich, USA).
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bioRxiv - Neuroscience 2024Quote: ... A perfusion chamber of 20 mm diameter and 2.5 mm height (Grace Bio-Labs CoverWell, Millipore Sigma, Cat. #GBL622301) with an adhesive base and two 1.5 mm ports (with attached tubing connectors ...
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bioRxiv - Molecular Biology 2024Quote: ... and was subcloned into these sites in the pET-23b expression vector (Novagen, Madison, WI, USA). The resulting pET-I–ShaI vector encoded the I-ShaI protein with a six-histidine (His ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 1.0% NP-40) supplemented with 10 μM leupeptin (Sigma-Aldrich) and 1 mM phenylmethanesulfonyl fluoride (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: HEK293T cells were cultured in a DMEM medium (Sigma-Aldrich, D5648), supplemented with 4.6 g/L glucose and 10% fetal calf serum (Gibco ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... The primary antibodies used were mouse monoclonal anti-FLAG M2 (Sigma, F3165), monoclonal mouse anti-HSC70 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... then streaked onto microscopy slides covered with 0.1% low melting agarose (Sigma). Imaging was done using phase-contrast and fluorescent modes on a Leica DM IL LED (Leica ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and 1 mM phenylmethanesulfonyl fluoride (Sigma-Aldrich) and boiled at 98°C for 10 min with SDS sample buffer prior to SDS-PAGE ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and the other half was probed with an anti-ssDNA antibody (MAB3034, Millipore, 1:10000).
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bioRxiv - Molecular Biology 2024Quote: ... and were produced by Sigma Aldrich (for sequences, see Table S2). The qPCR reaction mixtures contained 7-7 nmoles of forward and reverse primers ...
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bioRxiv - Molecular Biology 2024Quote: ... The aggregates were removed from the supernatant using a 0.45 µm pore size 96 well filter plate (Millipore, cat#MSHVN4550). The flow-through was transferred into a new plate ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used: anti-KRT5 (Rabbit, Sigma SAB4501651, 1:200), anti-acetylated α Tubulin (6-11B-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 pmol probe (IDT) and 10 pmol NDP1 primer (Sigma) (see sequences in Table S4 ...