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Citations for Millipore Sigma :
5201 - 5250 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Cells were arrested in prometaphase by incubating them for 2 – 4 h in 200 ng ml-1 nocodazole (Sigma-Aldrich, 31430-18-9). Mitotic exit was induced by adding flavopiridol (Tocris Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... this culture was incubated for a further 1 h before washing three times and RNA was extracted using Triazole (Sigma Aldrich, England, UK) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... DNA present in the homogenate was digested at 37°C for 1 h with 125 units benzonase (Sigma-Aldrich, St. Louis, MO, USA) before 70 µg of proteins were used for standard Western blotting ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in blocking solution and incubated for 1 h at room temperature (anti-E-cadherin, Cell Signaling Technology, 96743; anti-pan-cytokeratin, Millipore Sigma, C5992 ...
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bioRxiv - Cell Biology 2024Quote: Paraffin sections from the mid-regions of the Swiss rolls underwent Hematoxylin & Eosin (H&E) staining (Harris Hematoxylin, Electron Microscopy Science, 26754-01; Eosin, Sigma HT110116) for standard pathology evaluation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were digested at 24 °C for 1 h in EMMS-Ca2+ supplemented with 5 mg/mL lysing enzyme (Sigma-Aldrich, L3768-1G), 3 mg/mL zymolase (nacalai tesque ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in LB broth to an OD600 of 0.1 and 5 μl used to inoculate 96-well culture plates containing 150 μl LB agar supplemented with CPRG (Chlorophenol red-β-D-galactopyranoside – Sigma) (20 μg/ml) ...
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bioRxiv - Immunology 2021Quote: Fresh resected NSCLC patient tumors were gently dissociated mechanically in RPMI 1640 media containing 10 U/mL Collagenase D (Sigma Aldrich) and 25 ug/mL DNase I grade II (Sigma Aldrich ...
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bioRxiv - Immunology 2021Quote: ... Brains were placed in HBSS (w/ divalent cations Ca2+ and Mg2+) supplemented with actinomycin D (1:1000, 1 mg/mL, Sigma, #A1410), and microglia was isolated with the Papain-based Neural Tissue Dissociation Kit (P ...
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bioRxiv - Developmental Biology 2020Quote: ... The cells were cultured at 37°C overnight and induced by addition of 0.2 mM isopropyl-1-thio-β-d-galactoside (Sigma-Aldrich) for 4 hours at 28°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cultured in 96-well tissue culture plates and after 24 hours were treated with Actinomycin D 100nM (Sigma Aldrich) as positive control ...
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bioRxiv - Neuroscience 2022Quote: ... the cortical tissues were mechanically dissociated with pipette into single cells and were seeded on poly-D-lysine (Sigma Aldrich, 7405) coated T75 culture flask in Astrocytes medium (DMEM/F12 (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibodies goat anti-Olig2 (1:100, R&D AF2418) and rabbit anti-NeuN (1:100, Millipore ABN78) for two days at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting devices were again plasma-cleaned for 2 min right before coating with 25 µg/ml poly-D-lysine (PDL, P6407 Sigma Aldrich). After >30 min ...
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bioRxiv - Neuroscience 2020Quote: ... the cells were immediately transferred into pre-warmed MEM medium and seeded onto glass coverslips coated with a mixture of 100 μg/mL poly-D-lysine (Sigma-Aldrich) and 10 μg/mL laminin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Three days later the cultured neurons were re-suspended gently with 1 ml pipette and replated onto glass coverslips coated with a mixture of 100 μg/ml poly-D-lysine (Sigma-Aldrich) and 10 μg/ml laminin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 2D viability control cultures were seeded in 12-well cell culture plates on wells coated with poly-D-lysine (Sigma-Aldrich) at a density of 288,000 cells/well.
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bioRxiv - Biophysics 2022Quote: ... and incubated at 37 °C for 2 mins before the addition of D-ribulose 1,5-bisphosphate sodium salt hydrate (RuBP, Sigma Aldrich, US) final concentration 0.04 mM ...
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bioRxiv - Developmental Biology 2022Quote: ... and 115% wt/vol) were generated by dissolving D-(-)-fructose (JT Baker) in Milli-Q water with 0.5% α-thioglycerol (Sigma-Aldrich) to prevent browning and 0.02% sodium azide to prevent fungal infection in the higher concentration solutions ...
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bioRxiv - Immunology 2021Quote: ... overnight under gentle agitation at 4°C and eluted with lectin elution buffer containing 1 M Methyl a-D-mannopyranoside (M6882, Millipore Sigma). The resulting solution was dialyzed in PBS and further purified by size exclusion chromatography using Sephacryl S-500 HR resin.
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bioRxiv - Genomics 2020Quote: ... in situ cDNA synthesis reaction was performed by the addition of 75μl RT reagents: 50ng/μl actinomycin D (Sigma-Aldrich, USA), 0.5mM dNTPs (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Gentle pressure was applied to epididymis tissue using forceps and sperm were allowed to swim out during a 45-minute incubation in HTF + 0.75mM methyl-β-cyclodextrin (Millipore MR-070-D, Sigma C4555) at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: Cells were allowed to adhere for 2 days to glass chamber slides (PEZGS0816) coated with poly-D-lysine (Sigma #P-0899). Slides were fixed for 15 minutes with 4% PFA in PBS ...
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bioRxiv - Immunology 2021Quote: ... Perfused lungs were cut into small pieces and digested with 1 mg/mL collagenase D (Sigma-Aldrich, St. Louis, MO, USA) and 100 μg/mL DNAase (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated neurons were plated at a density of 500,000-600,000 cells per 60-mm dish on poly-D-lysine and laminin (Sigma-Aldrich, P6407, 11243217001) pre-coated 18 mm 1.5H coverslips (Marienfeld-Superior ...
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bioRxiv - Neuroscience 2020Quote: ... and the pellet was resuspended in DMEM and 10% FBS and plated in T75 flasks that had been pre-coated with poly-D-lysine (Millipore Sigma) and rinsed with water ...
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bioRxiv - Biochemistry 2020Quote: ... HLCs were exposed to either 5-Aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate (AICAR; 1mM; Sigma-Aldrich, A1393-50MG), O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA ...
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bioRxiv - Neuroscience 2021Quote: ... the electrode surfaces of 6-well Axion plates (Axion Biosystems, CytoView MEA 6) were coated with 10 mg/mL poly-D-lysine (Sigma, P7280) at room temperature overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Equal concentrations of serially diluted lysate (in the range of 0-60 μg/ml) from control and Thap1 cKO genotypes was incubated with 10 mM 4-methylumbelliferyl β-D-glucuronide (MUG, Sigma) in 0.2M Sodium acetate buffer ...
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bioRxiv - Neuroscience 2021Quote: ... one cell per well was manually plated into 96 well plates previously coated with Poly-D-Lysine (Sigma, 10 µg/ml) and Fibronectin (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated cortical neurons from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Neuroscience 2021Quote: ... mechanically dissociated cortices from P1-3 Wistar rats were cultured in poly-D-lysine (PDL)-coated (10 μg/mL; Sigma P7886) tissue culture plates (diameter 10 cm ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM ATP (Sigma Aldrich)] and then incubated with gently sonicated anti-FLAG beads to allow binding for 1 hr at 4°C on a rotator in the presence of oxygen scavenging reagents (5 mg/mL b-D-glucose (Sigma Aldrich), 0.25 mg/mL glucose oxidase (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2020Quote: ... After that, the sample of benzalkonium chloride was prepared by dissolving 10 mg in 550 μL D2O (D, 99.9 %) (Sigma-Aldrich, Norway) and transferred to 5mm LabScape Stream NMR tube ...
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bioRxiv - Biochemistry 2021Quote: ... Bacteria were grown to an OD600 of 0.8 in lysogenic broth (LB (Luria/Miller)) medium (Carl Roth) before the expression was induced with 0.5 mM isopropyl-β-D-thiogalactopyranoside (IPTG, Sigma-Aldrich). Following overnight expression at 18°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated onto collagen-coated plates in Opti-MEM for 3 hours and subjected to respective treatment conditions of either glucose (D-(+)-Glucose (Sigma Aldrich) or erythropoietin (Roche) ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was washed with 40 bed volumes of buffer A and the fusion protein was eluted with buffer A containing 5 mM d-Desthiobiotin (Sigma-Algrich). Peak atEH domain fractions were dialyzed overnight at 4°C against buffer A in the presence of TwinStrep-tagged TEV protease (32 ...
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bioRxiv - Cell Biology 2021Quote: Mature oocytes mounted in oil on a 22 by 40mm coverslip and set up under the microscope were treated with one or two drops of 10 µg/ml cytochalasin-D (Sigma-Aldrich) or 5% 1,6 hexanediol (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... limb buds from E12.5 mouse embryos were dissected and digested in 1 ml 1X HBSS (without magnesium, calcium, or phenol red) containing 1 mg/ml collagenase D (Millipore Sigma) and 50 µg/ml DNase I (Roche ...
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bioRxiv - Bioengineering 2020Quote: Cells were cultured in 75 cm2 flask without coating for Hela and SH-Sy5Y cells or with 10 μg/ml poly-D-Lysine coating (PDL, Sigma-Aldrich) for PC12 cells and maintained at 37 °C in a saturated humidity atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Neuroscience 2022Quote: ... Dissociated cortical cells from E18 Sprague Dawley rat pups were plated on slips or plates coated with poly-D-lysine (Sigma, MO). For live imaging ...
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bioRxiv - Cell Biology 2022Quote: CD15+ neutrophils were seeded at 20,000 cells per well in in HBSS with or without 15 μM Cytochalasin D (Sigma Aldrich, Burlington) black walled 96 well plates (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... a single whole spheroid was transferred just prior to recording into a well of a 6-well MEA with 64 low-impedance platinum microelectrodes (Axion Biosystems, Atlanta, GA USA) previously coated with 100 μg/ml poly-d-lysine (Sigma-Aldrich) and 10 μg/ml Laminin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... The 28.2-mm culture dish (Nest, 801001) with 15-mm glass bottom is pre-coated with poly-D-lysine (10ug/ml, Sigma, 32160801) for 20-24 hours and wash three times with water to remove redundant poly-D-lysine ...
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bioRxiv - Microbiology 2022Quote: ... Purified neurons were counted and plated on 10-mm coverslips pretreated with poly-D-lysine (Sangon Biotech) and laminin (Sigma-Aldrich). About 6,000 neurons were plated on each coverslip ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins transferred to nitrocellulose membranes were probed with either affinity-purified polyclonal rabbit anti-Gli326 or goat polyclonal anti-Gli3 (R&D, AF3690) and mouse anti-vinculin (Sigma, V9264) and visualized with fluorescent secondary antibodies (LI-COR IRDye 800CW ...
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bioRxiv - Immunology 2022Quote: ... 50% of the medium was replaced with growth medium additionally supplemented with 4 μM cytosine-1-β-D-arabinofuranoside (Sigma-Aldrich).