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Citations for Millipore Sigma :
5451 - 5500 of 5771 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... reduced and alkylated according to the manufacturers protocol and digested overnight with trypsin (SERVA, Heidelberg, Germany) followed by 4 h of digestions with chymotrypsin (Sigma-Aldrich, St. Louis, USA) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... for 3 min at 95 °C, and then mixed with 50 μL hybridization buffer H (4x SSC, 4 mg/ml acetylated BSA (Sigma Fluka Aldrich, B8894-1.5mL), 20 mM vanadyl ribonuclease complex) ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% [v/v] Triton X-100) and incubated at 37°C for 3 h either with α-mouse plus and α-rabbit minus for PLA assay (Sigma-Aldrich, Duolink). PLA samples were then washed with MTSB and incubated for 3 h at 37°C with ligase solution ...
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bioRxiv - Neuroscience 2023Quote: Rescue of cholesterol accumulation were performed by adding the drugs to cultures previously incubated overnight with Bodipy-cholesterol and further incubated for 6 h at the following concentrations: Evodiamine (Sigma, stock 1000x in DMSO) 10 µM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were further incubated in medium containing 300 nM tamoxifen for 48 h and then incubated with two-fold dilutions of AdV-C5-EGFP stock virus in DMEM medium (Sigma-Aldrich, cat. no. D6429) supplemented with 7.5% fetal calf serum (Gibco/ Thermo Fisher Scientific ...
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bioRxiv - Genetics 2022Quote: ... 2% normal goat or donkey serum and 0.1% Triton X-100 in PBS (-) at room temperature for 1 h and reacted with mouse anti-parvalbumin monoclonal antibody (Sigma-Aldrich, P3088, 1:4000), goat anti-somatostatin antibody (Santa-Cruz ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: The cells were then incubated at 37°C for 4 h in the presence of 25 μM 5-iodo-2′-deoxyuridine (IdU; Sigma-Aldrich, St. Louis, MO). After washing cells with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... neurospheres were collected or attached to standard culture plates in media containing 5% FBS for 6 h and stained with crystal violet solution (Sigma-Aldrich, 548-62-9).
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bioRxiv - Bioengineering 2024Quote: ... and transfected 24 h with EGFP-fused mouse brain membrane protein IgSF8 (Uniprot G3UYZ1) using X-treme GENE 9 DNA Transfection Reagent (Sigma Aldrich, cat. no. 6365787001). Cells were lysed for 2 h at 4 °C on a rocking platform with 0.5 mL per well of 20 mM HEPES pH 7.5 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... incubated in 2 N HCl (30 ℃, 30 min), blocked (37 ℃, 1 h) in 1% donkey serum albumin (DSA) and 0.25% Triton-100 (Sigma-Aldrich, St. Louis, MO, USA), rinsed three times (each 5 min ...
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bioRxiv - Physiology 2024Quote: ... for 5 min at a speed of 20 mL/h in accordance with our published protocol.10 Fifty µM adenosine diphosphate (ADP) (Sigma-Aldrich, cat no. A2754) was added directly to the perfusing of one channel per slide as positive control ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were incubated for 1 h at RT with BSA blocking buffer supplemented with anti-alpha-tubulin monoclonal antibodies (DM1a (Sigma T-6199, mouse origin), diluted 1:1000 and Yol1/34 (Bio-rad MCA78G ...
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bioRxiv - Cell Biology 2020Quote: ... at pressure range 25 – 30 kPsi with subsequent addition of 0.5 % w/v Triton X-100 (Cavin1 purification) or n-dodecyl β-D-maltoside (DDM) (1.2 mM) (MBP-GBP-CAV1 purification) and 5 mM imidazole (Sigma Aldrich, 792527) followed by centrifugation 35,000x g for 30 min ...
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bioRxiv - Developmental Biology 2021Quote: ... cells from the ATOH1-EGFP line were gently scraped off Transwell membranes and plated on poly-D-lysine (Millipore, A-003-E) and Laminin (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... HEK293T and U-2 OS cells were seeded on to poly-D-lysine (Cat. No. A-003-E, Sigma, St. Louis, USA) pre-coated 24-well plates at 24 h prior to transfection.
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bioRxiv - Neuroscience 2022Quote: ... DRGs from E13.5 embryos were dissected and plated as explants in chambers coated with 10 μg/ml poly-D-ly-sine (Sigma Millipore, catalog #P6407) and 10 μg/ml laminin (Sigma Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Cells from each pup brain were plated onto an individual T75 flask pre-treated with poly-D-lysine (Sigma-Millipore, cat. #P6407), to obtain an enriched astrocyte population ...
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bioRxiv - Neuroscience 2021Quote: ... Cells from each pup brain were plated onto an individual T75 flask pre-treated with poly-D-lysine (Sigma-Millipore, cat. #P6407), to obtain an enriched astrocyte population ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subcultured onto glass coverslips (hand-cut to ∼5 x 5 mm) coated with 0.1 mg/mL Poly-D-Lysine (Millipore-Sigma, #27964-99-4) in 35-mm cell culture dishes (Corning ...
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bioRxiv - Molecular Biology 2021Quote: Extracellular Gus activity was determined to quantify unconventional Cts1 secretion using the specific substrate 4-methylumbelliferyl ß-D galactopyranoside (MUG, Sigma–Aldrich). Cell-free culture supernatants were mixed 1:1 with 2x Gus assay buffer (10 mM sodium phosphate buffer pH 7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... 150K cells were plated in 24-well flat bottom plates on coverslips (12 mm, thickness ~0.1 mm) coated with Poly-D-Lysine (Sigma-Aldrich P7886-50MGP) in Minimum essential medium (MEM) ...
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bioRxiv - Molecular Biology 2020Quote: ... After 24 hours cells were treated with 100 μM 5,6-dichloro-1-bold beta-D-ribofuranosylbenzimidazole (DRB) (287891, Sigma St. Louis, MO) for 3 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryonic day 40 pancreata were diced finely with a razor blade and transferred into a 4°C solution of Collagenase D (Millipore-Sigma, Cat# C9263) at a concentration of 1 mg/ml in HBSS ...
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bioRxiv - Genetics 2020Quote: ... blood glucose was measured using a glucometer from tail tip blood before and multiple times within 2 hours of intraperitoneal injection of 2g/kg D-(+)-Glucose (Sigma Aldrich # G7528).
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bioRxiv - Genomics 2020Quote: ... 3 million CD4+ T cells were incubated in the presence of 5,6-Dichloro-1-β-D-ribofuranosylbenzimidazole (DRB, Sigma-Aldrich, Cat: D1916) at a final concentration of 65μM or Triptolide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... coated with poly-d-lysine (5 mg/50 ml distilled water for 2 hours, PDL; 135 kDa; Sigma-Aldrich, St. Louis, MO) or onto poly-d-lysine-coated BD BioCoatTM (BD Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... Probes were denatured for 5 min at 95 °C before their addition to the hybridization solution (10% w/v dextran sulfate, Sigma D-6001), 10% v/v deionized formamide (VWR 97062-008) ...
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bioRxiv - Bioengineering 2021Quote: ... we added ProxUp and ECGM2 media mixed at 1:1 ratio and containing an additional 5.5 mmol/L of D-Glucose (Sigma Aldrich, Cat# G5767) to all the wells on days 5 ...
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bioRxiv - Biochemistry 2021Quote: ... and GUS activity was measured after 30 minutes of incubation in the presence of 1 mM 4-methylumbelliferyl-β-D-glucuronide (MUG, Sigma Aldrich). Reactions were stopped by the addition of four volumes of 0.2 M Na2CO3 and fluorescence was measured in a Biotek Synergy microplate reader with excitation and emission wavelengths of 365 nm and 455 nm ...
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bioRxiv - Neuroscience 2020Quote: ... 48-well MEA plates (Axion Biosystems #M768-KAP-48) were coated with 50 µg/mL poly-D-lysine (Sigma-Aldrich #P0899-50MG) in borate buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... transferred onto nitrocellulose membranes and incubated overnight at +4 with primary antibodies against PROX1 (goat-anti-human, AF2727, R&D, 1:1000) and vinculin (mouse anti-vinculin, V9131, Sigma, 1:1000). HRP conjugated secondary antibodies (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293T stable cell line for HSPB8-V5 were grown on poly-D-lysine (P7280-5×5MG, Sigma-Aldrich, Saint Louis, MI, USA) coated glass coverslips ...
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bioRxiv - Systems Biology 2020Quote: ... rabbit anti-PDGFRα (1:400, gift from Prof Stallcup); goat anti-PDGFRα (1:200, R&D Systems; mouse anti-APC (CC1; 1:300, Millipore); rabbit anti-GFAP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... 1×105 cells were plated on coverslips that were pre-coated with 5 μg/ml poly-D-lysine (Sigma-Aldrich, Rehovot, Israel) in a 24-well plate containing 1.5 ml Neurobasal-A medium (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended and plated on 24-well plates containing cover slips coated with 10 μg/ml poly-D-lysine (Sigma-Aldrich, Germany) at a density of 0.8×105/ml in RPMI medium (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Each sample was diluted 1/10 with McIlvaine citrate-phosphate buffer pH 4.5 and activity assayed with 4-methylumbelliferyl-2-acetamido-2-deoxy-β-D-gluco-pyranoside (Sigma, Dorset, UK) in a plate reader.
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bioRxiv - Microbiology 2020Quote: The fluorescent substrate MUC was applied for the Cts1 liquid assay (4-methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside hydrate; M5639 Sigma-Aldrich) (Koepke et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... cell pellets were re-suspended in 1 mL cold D-PBS followed by the addition of 1 μL of aniline (Sigma-Aldrich 242284) and 10 μL biocytin hydrazide (Biotium 90060) ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293 cells transfected with either V2RWT or V2RT360A were seeded at a density of 0.2 million per well in a 24-well plate precoated with 0.01% poly-D-Lysine (Sigma-Aldrich; cat. no. P0899). After 24hr ...
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bioRxiv - Cell Biology 2022Quote: ... in a 24-well plate were coated with 4 mL per plate or 500 μL per well of 50 μg/ml poly-D-lysine (PDL, Sigma, catalog #: P6407) at 4°C overnight ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse macrophages J774A.1 cell line [NIHS (JCRB) 9108] were cultured in Dulbecco’s Modified Eagle Media (DMEM) (Sigma Aldrich #D-5030, USA) with 10% fetal calf serum and penicillin and streptomycin (Sigma Aldrich #F-7524 ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... 0.25% Triton-X 100) and 4-Methylumbelliferyl β-D-glucopyranoside stock solution (30 mM; Sigma-Aldrich, M3633-1G, stock solution in DMF) was diluted 3-fold in the GCase activity buffer ...
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bioRxiv - Cell Biology 2021Quote: ... We then centrifuged the homogenate at 210 g for 10 min at room temperature and resuspended the pellet in complete medium supplemented with 10 μM of cytosine- β-D-arabinofuranoside (Sigma-Aldrich) to prevent fibroblast growth ...
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bioRxiv - Genetics 2022Quote: ... cell pellets were re-suspended in 1 mL cold D-PBS followed by the addition of 1 μL aniline (Sigma-Aldrich 242284) and 10 μL biocytin hydrazide (Biotium 90060) ...
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bioRxiv - Cell Biology 2022Quote: ... 150 mM NaCl, 2 mM MgSO4, 0.2% n-dodecyl-β-D-maltoside [Nacalai Tesque, 14239-54] with protease inhibitor [Sigma-Aldrich, P8340]). After 20 min of incubation on ice ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL of cell lysate was added to 45 μL of 10 mM 4-methylumbelliferyl-β-D-glucuronide solution (MUG, M-9130; Sigma-Aldrich). The reaction was stopped after 1 hour incubation at 37°C by adding 220 μL of 0.2 M Na2CO3 ...
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bioRxiv - Developmental Biology 2022Quote: ... The following chemicals were prepared and used according to the manufacturers’ guidelines: 2-deoxy-D-glucose (2-DG, Sigma, cat. no. D8375), Na-iodoacetate (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... calcein (C, >95%), D-cycloserine, fluorescein (F, 95%), fluorescein-5-isothiocyanate (FITC, 90%) and TRITON® X-100 were purchased from Sigma-Aldrich. Ethanol (for UV spectroscopy ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated overnight at 4 °C with anti-hACE2 antibody (1:1000; R&D AF933) and anti-beta actin (1:2000; CST 4970) in 5% BSA (Sigma-Aldrich A9647) in TBST ...