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Citations for Millipore Sigma :
451 - 500 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 0.25% NaDoc and 1% NP-40) supplemented with protease inhibitor 1X (SIGMAFASTTM) and phosphatase inhibitor 1X (Sigma) and kept on ice for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... and then lysed on ice in lysis buffer (10 mM Tris-HCl pH 8.0; 10 mM NaCl; 0.5% (v/v) NP-40 substitute (Sigma); 1% (v/v ...
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bioRxiv - Microbiology 2022Quote: ... lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.25% deoxycholic acid, 1% NP-40, 1 mM EDTA; Millipore) supplemented with 1× protease inhibitor cocktail (Thermo Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... Specimens were prepared by dissolving AEMA powder and lyophilized AuMA NPs in 0.6 mL of D2O (Sigma-Aldrich) at a concentration of 10 and 30 mg/mL ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... 0.1% NP-40) and eluted in 43 µl of a 200 mg/ml dilution of FLAG peptides (Sigma) in 1x TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% NP-40 substitute (Fluka)) and added to 5-7 µL of protein A sepharose beads (Sigma) in a 50% slurry from the vendor that had been washed in IPP150 and conjugated to ∼5-10 µg of affinity-purified or protein A-purified anti-Prp43p ...
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bioRxiv - Immunology 2019Quote: ... Cells were spun at 1,000 g for 5 minutes at 4C and resuspended in NP-40 lysis buffer (10 mM Tris-HCl [pH 7.5], 0.05% NP40 [Sigma] ...
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bioRxiv - Immunology 2019Quote: ... mice were immunised with 25 μg NP-LPS and injected intra-peritoneally with 1 mg Edu (Sigma-Aldrich) and maintained on drinking water containing 500 μg/ml Edu and 1% glucose during 6 days ...
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bioRxiv - Plant Biology 2019Quote: ... 25mM Tris pH 7.5, 1mM EDTA, 150mM NaCl, 0.1% NP-40, 10mM DTT and 1x protease inhibitor cocktail, Sigma). Western blots were incubated overnight with GFP-antibody (Santa Cruz Biotechnology Inc ...
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bioRxiv - Developmental Biology 2020Quote: ... pH 7.4, 10 mM MgCl2, 150 mM KCl, 1% NP-40 [Sigma I8896-50ML], 0.5 mM DTT [Sigma, 646563] ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were lysed for 30 min at 4°C on the wheel with RIPA buffer (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 1% NP-40, 0.5% deoxycholic acid, 0.1% SDS, 2 mM MgCl2; Sigma-Aldrich) supplemented with proteases and phosphatases inhibitors and 50 mM 2- Chloroacetamide to inhibit the de-ubiquitinases ...
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bioRxiv - Bioengineering 2021Quote: ... NPs were purified by centrifugation over a filter (molecular weight cutoff of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg mL−1 ...
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bioRxiv - Bioengineering 2021Quote: ... NPs were purified by centrifugation over a filter (molecular weight cutoff of 10 kDa; Millipore Amicon Ultra-15) followed by resuspension in PBS to a final concentration of 200 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 150mM NaCl, 1% 5mM EDTA pH8, 1% NP-40, 0.5% Na deoxycholate, and 0.1% SDS; all from Sigma). Cells where left on ice during 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... 50,000 – 60,000 cells were lysed on ice by cold lysis buffer containing 0.1% NP-40 (Sigma-Aldrich, 98379), 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Lysis Buffer (150mM NaCl, 50mM Tris-Hcl, pH7.5, 10% Glycerol, 0.5%NP-40, 1mM MgCl2, 1/1000 Benzonase (Sigma), Phosphatase (PPI ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets were then gently resuspended in 50 µl of lysis buffer (48.5 µl resuspension buffer, 0.5 µl 10% NP-40 (Sigma) 0.5 µl 10% Tween-20 (Sigma) ...
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bioRxiv - Bioengineering 2022Quote: Cells were washed once with PBS and lysed in cold RIPA lysis buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1x protease inhibitor cocktail [Sigma]). Lysate was sonicated (Qsonica Q800 Sonicator ...
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bioRxiv - Developmental Biology 2022Quote: ... were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40: Sigma). Transposition was performed directly on nuclei using 25 µl tagmentation reaction mix (Tagment DNA Buffer #15027866 ...
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bioRxiv - Bioengineering 2022Quote: The effective swelling ratio of gelMA and gelMA-Au NP hydrogels comprising 20% w/v gelMA and 10 mM Au NPs was measured by gravimetric analysis.3,47,48 Hydrogels were incubated in Dulbecco’s PBS (DPBS, Sigma-Aldrich) at 37°C for 24 h to reach the equilibrium swelling ...
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bioRxiv - Genetics 2022Quote: ... 0.05% NP-40) + protease inhibitors (Complete Ultra Tablets, Mini Protease Inhibitor Cocktail; Roche #05892791001 and 1mM PMSF; Sigma). Worms were allowed to settle at the bottom of the tube and the lysis buffer was removed until there was 0.5-1ml buffer covering the worm pellet ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... Cells were then lysed with ice-cold lysis buffer (25 mM Tris, pH 7.4, 150 mM NaCl, 0.4% NP-40 (Sigma), 5% glycerol ...
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Control of cortical cytoskeleton-membrane interaction by RhoA regulates peripheral nerve myelinationbioRxiv - Neuroscience 2021Quote: ... 1% NP-40, 1 mM EDTA, 0,5% sodium deoxycholate, 0,1% SDS, 1% protease and phosphatase inhibitor cocktails [Sigma]). Samples were left on ice for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... NPs were purified by ultracentrifugation over a filter (molecular weight cut-off of 10kDa; Millipore Amicon Ultra-15) followed by resuspension in water to a final concentration of 200 mg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... NPs were incubated at 5 mg/mL in a 20 μg/mL Bovine Serum Albumin (BSA; Sigma-Aldrich) solution for 2 hours at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The ability of C-Mn3O4 NPs to prevent H2O2 mediated degradation of sodium containing dye RB (Sigma, USA) was used as an indicator of its H2O2 scavenging activity ...
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bioRxiv - Genetics 2020Quote: ... and suspended in 1 mL of ice-cold protein extraction buffer (50mM HEPES pH 7.5, 150mM NaCl, 0.02% NP-40, 1mM EDTA, 1mM phenylmethylsulfonyl fluoride [PMSF; Sigma, P7626] ...