Labshake search
Citations for Millipore Sigma :
701 - 750 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2 μg/ml Puromycin (Sigma–Aldrich). After 7 days of selection ...
-
bioRxiv - Cell Biology 2022Quote: ... Infectious media containing virus was collected 40 hrs later and filtered with a 0.45μm PES membrane filter (Millipore). Live filtered virus was used to transduce cells with polybrene (10μg/ml ...
-
bioRxiv - Microbiology 2022Quote: ... Thiol concentration at the same intervals as cells infected with virus was determined by Ellman’s reagent (5,5’-dithio-bis-2-nitrobenzoic acid, DTNB, Sigma) by reading the optical density at 412 nm in a plate reader (Biotek Cytation 5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the virus-containing supernatant was centrifuged 500 x g for 5min and filtered with 0.45um PES filters (Millipore), followed by ultracentrifugation (25,000rpm for 2h) ...
-
bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
-
bioRxiv - Microbiology 2021Quote: ... E2 (CHIK-102 and CHIK-263) [42] or Zika Virus E as a control (ZV-2, Sigma-Aldrich) (kindly provided by Dr ...
-
bioRxiv - Genetics 2020Quote: ... 10 μL of each virus was diluted in growth media containing polybrene (10 μg/mL; Sigma-Aldrich, USA) then mixed with cells ...
-
bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
-
bioRxiv - Genomics 2021Quote: ... We filtered and concentrated the virus in PBS using Amicon Ultra-15 centrifugation filters (Millipore, Burlington, MA; #UFC905024). The viral titer was measured with the AAVpro(R ...
-
bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
-
bioRxiv - Microbiology 2020Quote: ... The virus was passaged twice in Vero E6 cell line (ATCC) maintained in DMEM High Glucose (Sigma-Aldrich) supplemented with 2% heat-inactivated Fetal Bovine Serum (Thermo Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... Virus-containing supernatant was collected 48-72 hours post-transfection and filtered through 0.45um PES membrane filter (Millipore). For infection ...
-
bioRxiv - Immunology 2020Quote: ... and cells were overlaid with virus growth medium containing Avicel microcrystalline cellulose (final concentration of 1.2%) (Sigma-Aldrich). The plates were then incubated at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
-
bioRxiv - Microbiology 2022Quote: ... a pre-treatment of the virus with 10 μM of porcine trypsin (Millipore-Sigma, cat nr. T0303-1G) for 15 min at 37’C was included ...
-
bioRxiv - Cell Biology 2022Quote: ... were incubated in 1.5 mL virus-containing media supplemented with 0.5 mL fresh media and 0.8 μg/mL polybrene (Millipore Sigma). The 6-well plate was covered with aluminum foil to keep cells in the dark ...
-
bioRxiv - Microbiology 2022Quote: ... A27(-) and H3(-) virus stocks were generated in the absence of isopropyl β-d-thiogalactopyranoside (IPTG; Sigma 16758). Plaque forming units /ml and particle counts - calculated from the optical density at 260 nm44 - were determined for each purified recombinant VACV MV stock.
-
bioRxiv - Cancer Biology 2022Quote: ... filtered (0.45 µm) and CDX cells were infected with 1 mL virus containing 12 µg/mL polybrene (Sigma), followed by selection with Puromycin (Merck ...
-
bioRxiv - Cell Biology 2023Quote: ... Virus containing media was added to ATG3 KO HeLa cells with 8 μg/mL polybrene (Sigma-Aldrich, H9268). After a day ...
-
bioRxiv - Cell Biology 2023Quote: ... the virus-containing media was removed and N2 media with doxycycline (DOX, 2 µg/mL, Sigma, catalog #: D9891) was added ...
-
bioRxiv - Cancer Biology 2022Quote: ... The cells were transduced with lenti-shPGC1α RNA virus with 1:2000 dilution polybrene (Millipore, TR-1003-G) for 24 h and then media was changed ...
-
bioRxiv - Pathology 2023Quote: ... Stock virus was produced after three serial passages in Vero E6 cells using Dulbecco’s Modified Eagle’s Medium (DMEM; Sigma) supplemented with 2 % fetal bovine serum ...
-
bioRxiv - Microbiology 2023Quote: ... Virus-plasma mixture were aspirated and 2ml of 1% methylcellulose (combination of 2% methylcellulose (Sigma, Cat#435244-250G) and equal parts of 2X MEM (Gibco ...
-
bioRxiv - Microbiology 2023Quote: ... The virus suspension was immediately by passed through a Sephadex G-10 (Sigma-Aldrich, St. Louis, MO, USA) filter column via centrifugation (5 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... of p150 from rubella virus strain RVi/Brooklyn.NY.USA/98/1B CRS (GenBank JN635282; UniProt G3M8F4) was cloned into the pET24a plasmid (Novagen) with a C-terminal hexahistidine purification (His6 ...
-
bioRxiv - Immunology 2023Quote: ... virus-infected or HBSS-injected larvae were exposed to either dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO), DMSO-solubilized ramipril (Cayman Chemical Company ...
-
bioRxiv - Neuroscience 2024Quote: ... Virus was concentrated to a volume of 0.25 mL in a 100KD cutoff Amicon centrifugal filter (Millipore UFC910096) pretreated with a series of DPBS+ Pluronic F68 (Fisher 24-040-032 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The cell pellets were suspended in 500 µl of IP buffer (20 mM HEPES-KOH, pH 7.6, 125 mM potassium acetate, 1 mM EDTA, 20% glycerol, 1mM DTT, 1% NP-40, 1x Sigma protease inhibitor cocktail). One ml of acid-washed glass beads was added to each tube and the cells were lysed by bead beating for 8 minutes with incubation on ice for 3 minutes after each minute ...
-
bioRxiv - Neuroscience 2021Quote: ... The medium was replaced every 2-3 days until day 10 when each NP was individually plated for allowing neurite outgrowth on surfaces previously coated with 100 μg/mL poly-L-ornithine (Sigma Aldrich, P3655) and 20 μg/mL laminin (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: Cell lysates for immunoblot analysis were prepared from cells after functional analysis using NP-40 lysis buffer (1% Igepal, 150mM NaCl, 50mM Tris-HCl pH7.6) supplemented with 1× protease inhibitor cocktail (Sigma-Aldrich, cat #11836170001). Protein concentration was measured using a Coomassie (Bradford ...
-
bioRxiv - Microbiology 2020Quote: ... PBS with 1% (v/v) NP-40) supplemented with protease inhibitor cocktail (PIC; cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma-Aldrich). Cells were lysed using the VK05 lysis kit (Bertin ...
-
bioRxiv - Biochemistry 2021Quote: ... 10% glycerol, 1.5mM MgCl2, 0.5mM CaCl2, 0.5% NP-40, 1x SigmaFast protease inhibitor [Sigma], 1x PhosSTOP [Sigma], and 10units/mL DNaseI [NEB]) by rotating for 1 hour at 4°C ...
-
bioRxiv - Microbiology 2019Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: The supernatant (of Lipodisq NPs containing DMPC and DOX) was concentrated prior to size exclusion chromatography using a Vivaspin column (MWCO 100 kDa, Millipore (Burlington, MA)) and then applied to a 5 ml HiTrap column (GE Healthcare ...
-
bioRxiv - Plant Biology 2020Quote: ... After pre-washing the magnetic beads (50 mM Tris-HCl pH7.5, 500 mM NaCl, 5% glycerol, 0.05% NP-40, 1 mM MgCl2, 0.5 mM PMSF, 1X Sigma protease inhibitor, 10 mM DTT), the Co-IP sample (crude extract + αMyc antibody ...
-
bioRxiv - Microbiology 2021Quote: ... 1 mM EDTA, 0.5% NP-40, 1 tablet of Roche cOmplete Protease inhibitor Cocktail, and 1 tablet of Millipore Sigma PhosStop (4906845001). The lysate was sonicated 2 times for 5 seconds at setting 2 using a Fisher Scientific Sonic Dismembrator Model 100 with 1 min on ice in between ...
-
bioRxiv - Genomics 2021Quote: ... then the cells were lysed with Hi-C lysis buffer (10mM Tris-HCl pH8.0 10mM NaCl, 0.2% NP-40) with 1X of protease inhibitors (Sigma complete protease inhibitor cocktail) and the nuclei were recovered ...
-
bioRxiv - Microbiology 2020Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
-
bioRxiv - Cell Biology 2022Quote: ... refer to the publication from Susnik et al.32 Similar synthesis protocol was adapted for 59 nm SiO2-RhoB NP using a RhoB isothiocyanate (RBITC) dye (CAS 36877-69-7, Sigma-Aldrich, USA). For 119 nm SiO2-Cy5 NP synthesis ...
-
bioRxiv - Physiology 2022Quote: ... Cells were lysed with NP-40 lysis buffer and the cell lysates were immunoprecipitated with anti-Flag agarose (Sigma, St. Louis, MO). His-tagged TRPV2 and His-tagged TRPV2 (Y335F ...
-
bioRxiv - Cell Biology 2023Quote: ... and the remaining pellet was resuspended in lysis buffer (10 mM HEPES pH 7, 1 mM EDTA, 1% NP-40, 500 mM NaCl, Benzonase [Millipore] and PIC) and sonicated at 10-20% amplitude for three times ...
-
bioRxiv - Biochemistry 2023Quote: ... the NPs were purified by ultracentrifugation over a filter (molecular weight cutoff 10 kDa; Millipore Amicon Ultra-15, EMD Millipore, Burlington, MA) followed by re-suspending in Phosphate-Buffered Saline (PBS ...
-
bioRxiv - Bioengineering 2023Quote: Commercially available mesoporous silica nanoparticles (NPs) with a diameter of 500 nm and a pore size of 2 nm were used (Sigma-Aldrich, #805890). NPs were weighed and placed in glass scintillation vials with 1 mL of phosphate-buffered saline (PBS ...
-
bioRxiv - Cancer Biology 2022Quote: Protein extracts from cultured cells were prepared by using catenin lysis buffer [1%(v/v) Triton X-100 and 1%(v/v) NP-40 (Sigma-Aldrich, USA) in deionized phosphate-buffered saline (PBS)] supplemented with 1:7 proteases inhibitors cocktail (Roche Diagnostics GmBH ...
-
bioRxiv - Biochemistry 2023Quote: ... the cell pellet was re-suspended in ice cold PBS containing 0.1% Nonidet P-40 (NP-40) and 1% protease inhibitors (#P8340, Sigma-Aldrich, MI, USA). The samples were then centrifuged for 10 min at 10,000 rpm and the nuclear fraction was obtained as pellet ...
-
bioRxiv - Neuroscience 2023Quote: ... A subset of cells was rinsed twice with phosphate buffered saline and incubated with lysis buffer (1M Tris HCl [pH 7.5], NaCl, NP-40, and protease inhibitor cocktail [Sigma Aldrich, Catalog #P8340]) for 20 min at 4°C on a platform shaker ...
-
bioRxiv - Cell Biology 2023Quote: ... buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich], 1% sodium deoxycholate [Sigma-Aldrich] ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were lysed with NP-40 lysis buffer and lysates and bead fractions were collected and treated for 10 min with Benzonase (Sigma, E1014-25KU) to break up DNA/RNA ...
-
An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... Target cells were then transduced overnight with the filtered virus in the presence of 8 μg/mL polybrene (Millipore).
-
bioRxiv - Genetics 2021Quote: ... The medium was replaced 24 h later and the virus particles were collected and filtered through 0.45 μm filters (Millipore), 24 or 48 h later.