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Citations for Millipore Sigma :
601 - 650 of 1008 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected in lysis buffer (150mM NaCl, 50mM Tris-HCl pH 8, 1mM DTT, 0.5% NP-40, with protease and phosphatase inhibitors (Sigma).After 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... In brief, brain tissues were homogenised in NP40 lysis buffer (1M HEPES, 5M NaCl, 0.1 EDTA, 1% NP-40 (Sigma): pH = 7.6 ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by strong douncing in cell lysis buffer (KCL 85 mM, HEPES 5 mM, NP-40 0.5%, Sodium butyrate 10 mM, EDTA free protease inhibitor cocktail Sigma) following by 5 min centrifugation at 2000g ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 1 ml lysis buffer (5 mM PIPES pH 8, 85 mM KCl, 0.5% NP-40, protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Immunology 2024Quote: Cells were washed with ice-cold PBS and then lysed in 1% NP-40 lysis buffer (50mM Tris-HCI [pH 7.4], 150 mM NaCI, 1% IGEPAL CA-630 [Sigma]) supplemented with Halt Protease and phosphatase inhibitors for 15 min on ice (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... dissociation and lysed with elution buffer (150 mM NaCl - Sigma, 0.1% NP-40 - Sigma, 5 mM EDTA-Sigma, 50 mM HEPES - Sigma pH7.5 and protease inhibitor cocktail-Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 1mM PMSF, 1% Phosphatase inhibitors cocktail 2 Sigma-Aldrich Cat:P5726 ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were resuspended and lysed in 500 µl of ice-cold PBS containing 0.1% NP-40 (Sigma-Aldrich). The whole cell fraction (100 µl ...
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bioRxiv - Cell Biology 2023Quote: ... respectively for the indicated point times and lysed with 100μl extraction buffer (50mM Tris pH=8.0, 150 mM NaCl, 0.5mM EDTA, 1% NP-40, and 1mM DTT, and protease inhibitor cocktail [Sigma-Aldrich]). Cell extracts were sonicated and centrifuged at 4°C for 15 min at 16,000 ×g ...
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bioRxiv - Biochemistry 2023Quote: ... the nuclear lysate was obtained by resuspending the nuclear pellet in PBS containing 0.1% Nonidet P-40 (NP-40) and 1% protease inhibitor (#P8340, Sigma). Both the nuclear and cellular fractions were sonicated for 10 seconds of two cycles each with a holding period of 5seconds to cool down and then separated by SDS-PAGE and immunoblotted for TRF2 and TERF2IP ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested and lysed in NP-40 buffer supplemented with 10 mM N-ethylmaleimide (Sigma, catalog no.: E3876). Flag-immunoprecipitations were performed as described previously ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed for 20 min in 1ml of lysis buffer (50mM Tris, pH=7.4, 150mM NaCl, 1mM EDTA, 0.5% NP 40) supplemented by 1 mM DTT (Sigma-Aldrich) and 1x protease inhibitors (Roche Applied Science) ...
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bioRxiv - Plant Biology 2024Quote: ... anti-FLAG magnetic beads were washed 5 times with cold wash buffer (GTEN buffer plus 0.1 % NP-40 (Sigma) and Roche protease inhibitor (50 ml/ 1 tablet)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 0.5% NP-40, 50 mM NaF, 1 mM NaVO3, 1 mM dithiothreitol, 1 mM phenylmethylsulfonyl fluoride; all from Sigma). Total protein was separated on 10% SDS-PAGE reducing gels or 4%-20% detergent gels (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5 mM EDTA, 0.5% NP-40) and 2X native buffer (50 mM Tris-HCl, 10 mM dithiothreitol (Sigma, D9779), 10% glycerol (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... washed and resuspended in lysis buffer (50 mM Hepes pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.1% NP-40, 1 mM PMSF, 1× yeast protease inhibitor cocktail [Sigma]). Cells were lysed by using a bead beater (Biospec products ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
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bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
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bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...
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bioRxiv - Microbiology 2020Quote: ... Airway Chips infected with influenza virus (MOI = 0.1) were treated with 1 µM oseltamivir acid (Sigma-Aldrich) under flow (60 µl/h ...
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bioRxiv - Cell Biology 2021Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45μm filter (EMD Millipore) and used for transduction of MCF-10A cells in the presence of polybrene (8μg/mL) ...
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bioRxiv - Neuroscience 2021Quote: Each animal (with DREADD virus injection) received CLZ (0.5 mg/kg, Sigma-Aldrich, dissolved with 0.1% DMSO) or vehicle (sterilized saline with 0.1% DMSO ...
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bioRxiv - Immunology 2022Quote: ... the antibody-virus mixture was removed and 100 μl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cancer Biology 2022Quote: ... pLVX-EF1α-mKate2-WPRE-Neo virus supplemented with 10 µg/ml polybrene infection/transfection reagent (Sigma-Aldrich) was incubated with PSCs grown in monolayer for 24 h ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... virus was also concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore). Mice were injected s.c ...
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bioRxiv - Bioengineering 2021Quote: ... cells were transduced with 4 µL library virus/well along with 8 µg/mL Polybrene (Sigma-Aldrich) aiming for an MOI at 0.3-0.4 (Supplementary Methods and Results) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then cultivated with the virus in the presence of 4 μg/ml polybrene (Sigma-Aldrich) for 12 hr.
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with virus (MOI 0.5-1.0) and 5 µg/mL Polybrene (EMD Millipore, TR-1003). Polyclonal cells were selected for 2 weeks in 250 µg/mL G418 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Virus was then removed and cells were overlayed with 3 mL of DMEM with 0.8% methylcellulose (Sigma), 1% FBS ...
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bioRxiv - Physiology 2020Quote: Virus carrying the gene of interest was used to infect cell lines with 10mg/mL polybrene (Millipore). Media was replaced after 24 h and cells were sorted for GFP positivity after 48-72 h post-infection ...
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bioRxiv - Genetics 2019Quote: ... The resulting myoblasts were then infected with a retroviral cocktail containing Myf6-CTAP virus and polybrene (Millipore) for 8 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Suspensions of L-cells (from ATCC) for virus production were maintained in Joklik MEM medium (Sigma-Aldrich) supplemented with 1% L-Glutamine (Gibco.) ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant containing the virus was collected after 72 hours and filtered through a 0.22 micron PES (Millipore) filter ...
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bioRxiv - Genomics 2021Quote: ... Harvested media containing the desired virus were filtered through Millex-HV 0.45-µm PVDF filters (Millipore, SLHV033RS) and further concentrated with 100,000 NMWL Amicon Ultra-15 centrifugal filter units (Amicon ...
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...