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Citations for Millipore Sigma :
651 - 700 of 945 citations for Ebola Virus Nucleoprotein NP Sudan since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... supernatant containing propagated virus was filtered through an Amicon Ultra 15 (100 kDa) centrifugal filter (Millipore Sigma) at ~4000 rpm for 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... virus was purified using centrifugal filter devices (Centricon Plus-70 and Biomax 100.000, Millipore Corp., Bedford, MA) and stocks with a titre between 3×109 and 3×1010 viral genome equivalents (vge ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa or U2OS cells were transduced with virus for 48 h with 10 μg/ml polybrene (Sigma), then optimized for protein expression via fluorescence sorting or puromycin selection.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Microbiology 2022Quote: ... virus was preincubated for 1 hr at 4 °C with 100 μg/mL soluble H (Sigma H4784), HS (Sigma H7640) ...
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bioRxiv - Systems Biology 2022Quote: ... The virus was collected both 2 days and 3 days later and 0.45 μm syringe filters (Millipore) were used to eliminate cells ...
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bioRxiv - Molecular Biology 2023Quote: ... Virus containing media were harvested 48-64 h later and filtered by 0.45 μm Steriflip filter (Millipore). HepG2 and Huh7 cells were infected with 1 ml virus containing medium with 8 μg/ml polybrene for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Each SARS-CoV-2 virus was isolated after three passages in Vero cells in DMEM (Sigma Aldrich). Virus isolation was confirmed by RT-PCR using E gene-specific primer sets (Extended Data Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... the medium was replaced with virus-containing medium supplemented with 8 µg/mL of polybrene (Sigma-Aldrich). Approximately 24 hours post-viral treatment ...
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bioRxiv - Microbiology 2023Quote: ... Filtered virus-containing medium was mixed 1:1 with fresh HaCaT media and 1:1000 polybrene (Sigma), and used to transduce HaCaT cells overnight [75] ...
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bioRxiv - Immunology 2022Quote: ... The supernatant containing virus particles was collected after 48h and filtered via 0.45 μm filter (Roth/Millipore). Virus was concentrated using 20% sucrose and ultracentrifugation at 24,000 rpm (Beckman SW28 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... 2×106 cells were combined with 300 µL of virus and 0.8 µL/mL polybrene (Millipore, #TR1003G) in the well of a 12-well plate (final volume of 2 mL) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-15-Cas9 cells were infected with pooled CRISPR library virus using polybrene (8 µg/ml, Sigma) using a low multiplicity of infection (MOI = 0.75 ...
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bioRxiv - Microbiology 2023Quote: ... RNA from the supernatant of cell culture infected with the virus was isolated using the Trizol (Sigma) method described by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... NF1-MET cells were infected with 500 μL of 0.45 μm filtered virus with polybrene (EMD Millipore), and 48 hours post infection transduced cells were selected for by 2 μg/mL puromycin (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus was removed and replaced with normal growth medium containing 1 mg/ml puromycin from Sigma. The puromycin selection was used to kill off any uninfected control cells ...
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bioRxiv - Microbiology 2023Quote: ... the virus suspension was replaced with 1mL/well of overlay medium (OM) containing 1% methylcellulose (Sigma, USA) in MM ...
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bioRxiv - Microbiology 2024Quote: ... 100 TCID50 of the virus in Intesticult media supplemented with 500 μM sodium glycochenodeoxycholate (GCDCA; Sigma, G0759) was added to 5-day differentiated HIE monolayers in triplicate on a 96-well plate ...
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bioRxiv - Microbiology 2020Quote: ... The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo) containing 143 mM 2-Mercaptoethanol (Sigma-Aldrich). Western blotting using the elutes from affinity purification and the pre-purified input lysates were performed as described above with the following primary antibodies ...
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bioRxiv - Microbiology 2019Quote: ... The plate was washed three times with wash buffer (PBS/0.1% Tween-20) and the primary antibody (anti-NP mouse monoclonal Ab, Millipore, cat. MAB2851) added in blocking buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... 150 mM KCl, 1% NP-40 [Sigma I8896-50ML], 0.5 mM DTT [Sigma, 646563], 100 µg/ml cycloheximide [Sigma C4859-1ML]). Conjugated beads were then resuspended in low-salt buffer and mixed with 50 µg each of anti-GFP antibodies Htz-GFP-19C8 and Htz-GFP-19F7 (Memorial Sloan-Kettering Monoclonal Antibody Facility ...
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bioRxiv - Cell Biology 2021Quote: Cells treated with SPS either alone or in conjunction with bafilomycin-A1 were collected and subsequently lysed in lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 1% NP-40) supplemented with PhosStop phosphatase inhibitor cocktail (Sigma Aldrich) and protease inhibitor cocktail (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... gel-like NP tissues were carefully collected under aseptic condition and transferred to culture dishes with 0.1% type II collagenase (Sigma-Aldrich, USA) treatment for 4 h at 37°C to digest the tissue completely ...
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bioRxiv - Microbiology 2020Quote: ... and mutants (lpg1-/- lpg5A-/lpg5B-) were lysed in 1% NP-40 in the presence of an protease inhibitor cocktail (Sigma, 11836170001). Equal cell equivalents were separated on a 10% SDS PAGE (Biorad ...
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bioRxiv - Cell Biology 2020Quote: ... Embryonic tissues were ground to a fine powder using a mortar and pestle on dry ice and lysed in RIPA buffer (150M NaCl, 50mM Tris pH8, 5mM EDTA, 1% NP-40 (Sigma, I8896), 0.5% sodium deoxycholate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were treated for 2 h with 100 μM PALA and then lysed in NP40 Lysis Buffer (10 mM HEPES ph 7.4, 142 mM KCL, 5 mM MgCl2, 1 mM EGTA, 0.2% NP-40, and 1X Sigma protease inhibitor cocktail). Lysates were precleared with protein G-sepharose 4B beads (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfected cells were lysed for 20 minutes in pre-chilled Co-Immunoprecipitation buffer (50mM Tris-HCl pH 7.4, 150mM NaCl, 0.53% NP-40) supplemented with 1×protease inhibitor (Roche-Sigma, Cat no. # 11873580001) and 1×phosphatase inhibitor (Thermo Scientific™ ...
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bioRxiv - Microbiology 2021Quote: ... 1% NP-40) in presence of Protease cocktail inhibitor (cOmplete™, EDTA-free Protease Inhibitor Cocktail, Sigma Aldrich, catalogue number 11873580001). Protein lysates were left rotating on-wheel at 4 °C for 15 min and cleared by 15 min of centrifugation at 4°C at 13200 (max speed) ...
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bioRxiv - Immunology 2020Quote: ... as follows: 1mg/ml NP-CGG was mixed 1:1 with freshly prepared 10% Alum (31242, Sigma-Aldrich, St. Louis MO), pH adjusted to 6.5–7.0 ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were incubated in primary antibodies diluted in blocking buffer for 1hr (1:1000 PR8 NP polyclonal, gift from Dr. Adolfo Garcia-Sastre; 1:1000 γ-tubulin DQ-19, #T3195 Sigma; 1:1000 α-tubulin ...
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail, Sigma Millipore) was added to the thawed pellet ...
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bioRxiv - Cell Biology 2019Quote: Whole cell lysate was prepared in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Sigma Fast Protease Inhibitor cocktail) for EndoH digestion ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Sample preparation was done by drop-casting of C-Mn3O4 NP solution on 300-mesh amorphous carbon-coated copper grids (Sigma, USA) and allowed to dry overnight at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were isolated from the post nuclear fraction of the NP-40-lysed cells using magnetic beads loaded with Flag-specific monoclonal antibody (MAb) (Sigma, Mo) as described elsewhere (17) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed twice with PBS and lysed in 200µl RIPA buffer (150 mM sodium chloride, 1.0% NP-40 (Sigma-Aldrich, I8896-50ML), 0.5% sodium deoxycholate ...
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bioRxiv - Neuroscience 2023Quote: ... 100 mM KCl; 1% NP-40; 1 mg/ml sodium heparin; 1 mM DTT; 100 μg/ml cycloheximide [#C4859-1ML, Millipore Sigma] ...
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bioRxiv - Cell Biology 2023Quote: ... Knockdown efficiency and p130Cas over-expression levels were confirmed by Western blotting of cell lysates in radioimmunoprecipitation assay (RIPA) buffer (25 mM Tris-HCl, pH 7.5 [Sigma-Aldrich], 150 mM NaCl [JT Baker], 1% NP-40 [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 mM EDTA, 0.1 mM EGTA, 0.6% NP-40, 1 mM NaVO3, 1:500 Protease Inhibitors Cocktail from Sigma-Aldrich cat# P8340) for 20 minutes on ice in a shaker ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed with high-salt buffer (300nM NaCl, 50mM tris-HCl pH8, 10% glycerol, and 0.2% NP-40) supplemented with protease inhibitors (Sigma-Aldrich, #04693132001) and then were sonicated with a Bioruptor for 5min 30’’ on/off ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was resuspended in 250 μl of Cilia Final Buffer [without trehalose but with 44.1 µl of 10% NP-40 alternative (Millipore Sigma, 492016)] added to a final concentration of 1.5% NP-40] ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... Complementary DNA was reverse transcribed from 1 μg total RNA with Moloney murine leukemia virus reverse transcriptase (Sigma) using dNTP (Promega ...
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bioRxiv - Immunology 2021Quote: ... The swabs were placed into 2ml of virus transport medium (VTM) comprising tissue culture medium 199 (Sigma-Aldrich) supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100 μl of pre-warmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... the cell culture supernatants were pooled to concentrate the virus 20 times using 10,000 MWCO spin columns (Millipore), and aliquots were stored at −70°C [14] ...
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bioRxiv - Bioengineering 2020Quote: ... virus was collected after 120 hours from both cell lysates and media and purified over iodixanol (Optiprep, Sigma).
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bioRxiv - Biochemistry 2020Quote: ... P2 virus was then harvested by pelleting cells by centrifugation at 4000g and decanting into 5% FBS (Sigma), and then stored in the dark at 4°C ...